The Calpastatin Gene Polymorphism Study and Its Effect on Early Body Weight Traits in Zandi Sheep
محورهای موضوعی : Camel
ن. پیرویسی
1
(Department of Animal Science, Karaj Branch, Islamic Azad University, Karaj, Iran)
ع. نوشری
2
(Department of Animal Science, Karaj Branch, Islamic Azad University, Karaj, Iran)
ب. همتی
3
(Department of Animal Science, Karaj Branch, Islamic Azad University, Karaj, Iran)
کلید واژه: Zandi sheep, body weight traits, MspI, PCR-RFLP method, polymorphism of calpastatin gene,
چکیده مقاله :
The goal of this study was investigation of the calpastatin gene polymorphism and its effect on early body weight traits in Zandi sheep by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) method. Calpastatin is the main inhibitors of calpain-calpastatin enzyme system that play an important role in regulating the protein regeneration process. Genomic DNA was extracted by whole blood samples from 124 randomly selected Zandi breed sheep using salting out method. Birth weight, weight on 150 days old and daily weight gain from birth to 150 days old were recorded. The exon and intron 1 of ovine calpastatin gene were amplified to produce a 622 bp fragment. The PCR products were digested with MspI restriction enzyme and electrophoresised on agarose gel. Results showed 80.6% and 19.4% allele frequencies for A and B alleles and 65.32%, 4.04% and 30.64% genotype frequencies for AA, BB and AB genotypes, respectively. X2 and G2 tests showed the Hardy-Weinberg equilibrium for studied locus. The effect of calpastatin genotype on growth traits was insignificant, but genotype BB had the best performance among all the three traits, which indicates the better performance of normal allele (B) than mutant allele (A) for these traits.
هدف از این تحقیق بررسی چندشکلی ژن کالپاستاتین و تأثیر آن روی صفات وزن اولیه بدن در گوسفند زندی با استفاده از تکنیک PCR-RFLP بود. کالپاستاتین ممانعت کننده اصلی سیستم آنزیمی کالپاین-کالپاستاتین بوده و نقش مهمی در تنظیم مسیر پروتئین سازی بر عهده دارد. DNA ژنومی با استفاده از نمونه‌های خون کامل 124 رأس گوسفند زندی انتخاب شده به صورت تصادفی و با استفاده از روش استخراج نمکی تهیه گردید. همچنین وزن تولد، وزن 150 روزگی و افزایش وزن روزانه تولد تا سن 150 روزگی ثبت گردید. تکثیر ناحیه 622 جفت بازی اگزون و اینترون 1 ژن کالپاستاتین انجام شد. محصول PCR با استفاده از آنزیم برشی MSP I هضم و روی ژل آگارز الکتروفورز گردید. نتایج دو آلل A و B به ترتیب با فراوانی‌های 80.6 و 19.4 و سه ژنوتیپ AA، BB و AB به ترتیب با فراوانی‌های 65.32، 4.04 و 30.64 را نشان داد. آزمونهای X2 و G2 برقراری تعادل هاردی-واینبرگ در جایگاه مورد مطالعه را نشان داد. اثر ژنوتیپ کالپاستاتین روی صفات رشد غیرمعنیدار بود اما ژنوتیپ BB بهترین عملکرد را در هر سه صفت نشان داد که نشان دهنده عملکرد بهتر آلل نرمال (B) نسبت به آلل موتان (A) برای این صفات است.
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