Study of DGAT1 Gene Polymorphisms with Carcass Traits in Iranian Zel and Lori-Bakhtiari Sheep Breeds
محورهای موضوعی : Camel
م. صادقی
1
(Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran)
م. مخبر
2
(Department of Animal Science, Faculty of Agriculture, Urmia University, Urmia, Iran)
م. مرادی-شهر بابک
3
(Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran)
و. سلطانی
4
(Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran)
م. بهروزلک
5
(Department of Animal Science, Faculty of Agriculture, Payame Noor University, Tehran, Iran)
کلید واژه: Iran, Sheep, PCR-SSCP, Zel, <i>DGAT1</i> gene, Lori-Bakhtiari,
چکیده مقاله :
Diacylglycerol acyltransferase1 (DGAT1) plays an important role in the metabolism of triglycerides which catalyze the final step of triglyceride synthesis in animals. The objective of this study was to investigate the single-nucleotide polymorphisms (SNPs) in 5'UTR, exon-1, and exon-2 of DGAT1 in two Iranian indigenous sheep breeds. A total of 309 animals including fat-tailed Lori-Bakhtiari (n=152) and thin-tailed Zel (n=157) were used in this study. The genotypic patterns were detected by polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP). Five SSCP patterns were detected for 5'UTR and exon-1 fragment by PCR-SSCP and subsequently confirmed by sequencing PCR products. The sequencing results revealed that there are three novel polymorphisms in 5'UTR and exon-1fragment of DGAT1 at the studied breeds. Out of the detected polymorphisms only A277G substitution in exon-1 of DGAT1 leads to the changes in amino acids (p.Arg26Gly). There was significant correlation (p < 0.05) between fat-tail weight (FTW) and back-fat thickness (BFT) and the observed genotypes in Lori-Bakhtiari breed; therefore, animals with G5 pattern had higher FTW and BFT compared to G1 pattern. The G1 and G5 genotypic patterns or haplotypes were different at their position 101 of 5'UTR region. No significant relationship (p < 0.05) was found between the detected genotypes of 5'UTR and exon-1 fragment of DGAT1 in Zel breed and carcass traits. These results revealed that detected DGAT1 novel SNPs had significant effects on carcass traits and they can be used as a marker for these traits.
دی آسیل گلیسرول آسیل ترانسفراز1 (DGAT1) نقش مهمی در متابولیسم تریگلیسریدها دارد و مرحله پایانی مسیر سنتز تریگلیسرید در حیوانات را کاتالیز میکند. هدف مطالعه حاضر بررسی چندشکلیهای تک نوکلئوتیدی (SNPs) در نواحی 5'UTR، اگزون 1 و اگزون 2 در دو نژاد گوسفند بومی ایران بود. به این منظور، از تعداد 309 حیوان شامل گوسفند دنبه دار لری-بختیاری (n=152) و گوسفند بدون دنبه زل (n=157) استفاده شد. الگوهای ژنوتیپی با استفاده از تکنیک PCR-SSCP شناسایی شد. پنج الگوی ژنوتیپی در نواحی 5'UTR و اگزون 1 شناسایی شد. وجود تفاوت در الگوهای مختلف با استفاده از تکنیک توالییابی تأیید گردید. نتایج توالییابی حاکی از وجود سه پلیمورفیسم در نواحی 5'UTR و اگزون 1 نژادهای مورد بررسی بود. از میان نواحی دارای چندشکلی، تنها جایگزینی مربوط به موقعیت 277 در اگزون 1 منجر به تغییر اسید آمینه آرژنین به گلایسین شد (p.Arg26Gly). ارتباط معنیداری (P<0.05) بین ژنوتیپ‌های شناسایی شده در نژاد لری-بختیاری و وزن دنبه (FTW) و ضخامت چربی پشت (BFT) وجود داشت و حیوانات با الگوی ژنوتیپی G5 عملکرد بالاتری نسبت به الگوی ژنوتیپیG1 داشتند. این دو الگوی ژنوتیپی یا هاپلوتیپ تنها در موقعیت 101 ناحیه 5'UTR تفاوت دارند. هیچ ارتباط معنیداری (05/0P<) بین الگوهای ژنوتیپی مشاهده شده (قطعه 5'UTR و اگزون 1) و صفات لاشه نژاد زل مشاهده نشد. این نتایج نشان داد که SNPهای جدید شناسایی شده DGAT1 اثر معنیداری بر روی صفات لاشه دارند و میتوانند به عنوان نشانگر برای این صفات استفاده شوند.
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