The Study of Morphological Traits and Identification of Self-incompatibility Alleles in Almond Cultivars and Genotypes
محورهای موضوعی : Microbiology
Mousa Rasouli
1
(Department of Horticulture and Landscape, Faculty of Agriculture, Malayer University, P.O. Box 65719-95863 Malayer, Iran)
کلید واژه: Kernel, Prunus dulcis, Incompatibility, AS1II, AmyC5R, S-allele, Specific PCR,
چکیده مقاله :
The evaluation of an almond collection using morphological variables and identification of self-incompatibility genotype is useful for selecting pollinizers and for the design of crossing in almond breeding programs. In this study, important morphological traits and self-incompatibilities in 71 almond cultivars and genotypes were studied. Simple and multiplex specific PCR analyses were used in order to identify self-incompatibility alleles. Based on the results, cultivars and genotypes including ‘Dir Ras–e-Savojbolagh’, ‘D-124’, ‘D-99’, ‘Shahrood 12’, ‘Tuono’, ‘Nonpareil’, ‘Price’, ‘Mirpanj-e-Tehran’, ‘Pakotahe-e- Taleghan’, ‘V-13-34’, ‘V-16-8, ‘V-11-10’, ‘Zarghan 10’, ‘Uromiyeh 68’, ‘Barg dorosht-e-Hamedan’ and ‘Yazd 60’ were late flowering and had the highest quality of nut and kernel characters. The result of the PCR method using combined primers AS1II and AmyC5R showed amplification of ten self-incompatibility alleles (S1, S2, S3, S5, S6, S7, S8, S10, S12,and S unknown allele) and three Sfalleles. Moreover, S1 had the highest frequencies in comparison with other known S-alleles. Also, unknown alleles with different sizes were detected and 58 new bands were found in some cultivars.
Alonso JM, Socias I Company R (2006) Almond S-genotype identification by PCR using specific and non- specific allele primers. Acta Horticulturae. 726, 321-328.
Arteaga N, Socias I Company R (2001) Heritability of fruit and kernel traits in almond. Acta Horticulturae. 591, 269–274.
Baninasab B, Rahemi M (2007) Evaluation of three wild species of almond by the morphological characters. Journal of Central European Agriculture. 7(4), 619-626.
Boskovic R, Tobutt KR, Batlle I, Martínez-Gómez P, Gradziel TM (2003) Stylar rybonucleases in almond: correlation with and prediction of incompatibility genotypes. Plant Breeding. 122(1), 70-76.
Browicz K, Zohary D (1996) The genus Amygdalus L. (Rosaceae): Species relationships, distribution and evolution under domestication. Genetic Resources and Crop Evolution. 43(3), 229-247.
Doyle JJ, and Doyle JL (1987) A rapid isolation procedure for small quantities of fresh leaf tissue. Phytochem bull. 19, 11-15.
Fallah M, Rasouli M, Sharafi Y, Imani A (2014) Study of Compatibility Relationships Among Some Almond Cultivars and Genotypes Using of SAlleles Identification. Journal of Nuts. 5(2), 49-56.
FAO (2012) FAOSTAT database results. http://faostat.Fao.org.faostat. Servlet
Gulcan R (1985) Descriptor list for almond (Amygdalus amygdalus). Revised Edn.), International Board for Plant Genetic Resources, Rome.
Hajilou J, Grigorian V, Mohammadi SA, Nazemieh A, Romero C, Vilanova S, Burgos L (2006) Self- and Cross- (in) compatibility between important apricot Cultivars in northwest Iran. Journal of Hortrticultural Science and Biotechnology. 81(3), 513-517.
Halasz J, Hegedus A, Pedryc A (2006) Review of the molecular background of self-incompatibility in rosaceous fruit trees. International Journal of Horticultural Science. 12(2), 7-18.
Kester DE, Gradiziel TM, Almonds, in: Janick J, Moore JN (1996) (Eds.), Fruit Breeding. Vol. III. Nuts, John Wiley and Sons, New York. pp. 1-97.
Kester DE, Hansche PE, Beres W, Asay RN (1977) Variance components and heritability of nut and kernel traits in almond. Journal-American Society for Horticultural Science (USA). 102, 264–266.
Lopez M, Vargas FJ, Batlle I (2006) Self- (in) compatibility almond genotypes: A review. Euphytica. 150(1-2), 1-16.
Marchese A, Boškovic R, Martinez-Gomez P (2008) The origin of the self-compatible almond ‘Supernovae’. Plant Breeding. 127, 105–107.
Martínez-Gómez P, López M, Alonso JM, Ortega E, Sánchez-Pérez R, Batlle I, Socias i Company R, Dicenta F, Dandekar AM, Gradziel TM (2003) Identification of self-incompatibility alleles in almond and related Prunus species using PCR. Acta Horticulture. 662, 353-357.
Mousavi A, Babadaei R, Fatahi MR, Zamani Z, Dicenta F, Ortega E (2014) Self-incompatibility in the Iranian Almond Cultivar ‘Mamaei’ Using Pollen Tube Growth,Fruit Set and PCR Technique. Journal of Nuts. 5(2), 1-10.
Mousavi A, Fatahi Moghadam MR, Zamani Z, Imani A (2010) Evaluation of quantitative and qualitative characteristics of some almondcultivars and genotypes. Iranian Journal of Horticultural Sciences. 41(2), 119-131. [In Persian].
Mousavi A, Fatahi MR, Zamani Z, Imani A, Dicenta F, Ortega E (2011a) Identification of selfincompatibility genotypes in Iranian almond cultivars. Acta Horticulture. 912, 303-3011.
Mousavi A, Fatahi R, Zamani Z, Imani A, Dicenta F, Ortega E (2014) Genetic variation and frequency of S-alleles in Iranian almond cultivars. Acta Horticulture. 1028, 45-48.
Mousavi A, Fatahi Moghadam MR, Zamani Z, Imani A, Ortega E, Dicenta F (2011b) Identification of self-incompatibility alleles in Iranian almond cultivars and genotypes using PCR. Iranian Journal of Horticultural Sciences. 42(2), 169-183. [In Persian].
Ortega E, Boskovic RI, Sargent DJ, Tobutt KR (2006)Analysis of SRNase alleles of almond (Prunus dulcis): characterization of new sequences, resolution of synonyms and evidence of intragenic recombination. Molecular Genetics and Genomics. 276, 413-426.
Ortega E, Mousavi A, Dicenta F (2011) Cloning and Characterization of Nine New S-RNases from Iranian Almond Cultivars. Acta Horticulture. 912, 593-599.
Ortega E, Sutherland BG, Dicenta F, Boskovic R, Tobutt KR (2005) Determination of incompatibility genotypes in almond using first and second intron consensus primers: detection of new S alleles and correction of reported S genotypes. Plant Breeding. 124, 188-196.
Rahemi AR, Fatahi R, Ebadi A, Taghavi T, Hassani D, Gradziel T, Chaparro J (2010) Genetic variation of S-alleles in wild almonds and their related Prunus species. Australian Journal of Crop Science. 4(8), 648-659.
Sabeti H (1994) Forests, Trees, and Shrubs of Iran, Iran University of Science and Technology Press, Tehran. pp. 400. [In Persian]
Sánchez-Pérez R, Dicenta F, Martínez-Gómez P (2004) Identification of S-alleles in almond using multiplex-PCR. Euphytica. 138, 263-269.
Sánchez-Pérez R, Ortega E, Duval H, Martínez-Gómez P, Dicenta F (2007) Inheritance and relationships of important agronomic traits in almond. Euphytica. 155, 381-391.
Sepahvand E, Khadivi-Khub A, Momenpour A, Fallahi E (2015) Evaluation of an almond collection using morphological variables to choose superior trees. Fruits. 70, 53–59.
Shahmoradi M, Rasouli M, Ghazvini RF, Imani A, Hamidogli Y (2013) Discrimination and preliminary selection of self-compatible progenies among. Journal of Nuts. 3(3), 47-54.
Sheikh-Alian A, Vezvaei A, Ebadi A, Fatahi-Moghadam MR, Sarkhosh A(2010)Determination and identification of self-incompatibility alleles in selective Iranian and foreign almond (Prunus dulcis M.) cultivars by PCR Method. Iranian Journal of Horticultural Sciences. 41(3), 247-252. [In Persian].
Socias I Company R, Alonso JM (2004) Cross-incompatibility of Ferralis and Ferragnes and pollination efficiency for self-compatibility transmission in almond. Euphytica. 135, 333-338.
Spiegel-Roy P, Kochba J (1974) The inheritance of bitter and double kernel characters in the almond. ZPflanzenzuchtg. 71, 319–329.
Tamura M, Ushijima K, Gradziel TM, Dandekar AM (1999) Cloning of genomic DNA sequences encoding almond (Prunus dulcis) S-Rnase genes. Plant Physiology. 120, 1206-1210.
Tamura M, Ushijima MK, Sassa H, Hirano H, Tao RT, Gradziel TM, Dandekar AM (2000) Identification of self-incompatibility genotype of almond by allele–specific PCR analysis. Theoretical and Applied Genetics. 101,344–349.
Valizadeh B, Ershadi A (2009) Identification of self-incompatibility alleles in Iranian almond cultivars by PCR using consensus and allele-specific primers. Journal of Hortrticultural Science and Biotechnology. 84, 285-290.
Wirthensohn M, Rahemi M, Fernández i Martí A (2011) Identification of self-incompatibility genotypes and DNA fingerpriting of some Australian almond cultivars. Acta Horticulture. 912, 561-566.
Wunsch A, Hormoze JI (2004) S-allele identification by PCR analysis in sweet cherry cultivars. Plant Breeding. 123, 1-6.
Yamane H, Tao R, Sugiora A (1999) Identification and cDNA cloning for S-RNases in self-incompatible Japanese plum (Pruns Salisina cv, sordum). Plant Biotechnology. 16(5), 389-396.
Zeinolabedini M, Khayam-Nekoui M, Imani A, Majidian P (2012) Identification of self-compatibility and self-incompatibility genotypes in almond and some Prunus species using molecular markers. Seed and Plant Improvment Journal. 28-1(2), 227-238. [In Persian].
