Effects of methanol root extract and fractions of Waltheria Indica (linn) on sexual orientation and sexual behavioral parameters in male wistar rats
الموضوعات : مجله گیاهان دارویی
Sunday Yakubu
1
(Department of Pharmacology and Toxicology, Faculty of Pharmaceutical Sciences, University of Jos, Nigeria;)
Sunday Otimenyin
2
(Department of Pharmacology and Toxicology, Faculty of Pharmaceutical Sciences, University of Jos, Nigeria;)
Samuel Otokpa
3
(Department of Pharmacology and Toxicology, Faculty of Pharmaceutical Sciences, University of Jos, Nigeria;)
الکلمات المفتاحية: Medicinal Plants, fractions, mount, Aphrodisiac, intromission, ejaculation,
ملخص المقالة :
Background & Aim: Waltheria indica is a shrub whose roots are used in many communities of Northern Nigeria to manage several sexual behavioral disorders; however, there is no scientific study to verify this claim. The aim of the study is to determine the effect of methanol root extract of Waltheria indica and its fractions (ethyl acetate, n-butanol, and residual ethanol) on sexual orientation and sexual behavioral parameters in male albino rats.Experimental: Male rats were divided into five groups each containing 6 rats. Group 1 rats were treated with normal saline. Rats in Groups 2, 3 and 4 were treated with 50, 100 and 200 mg/kg crude extracts while rats in Group 5 were treated with Sildenafil 5 mg/kg. Treatment was administered once daily for 7 days. Female rats were artificially brought to oestrus by administering oestradiol 25 μg/kg and progesterone 500 μg/kg 48 and 8 h respectively prior to mating The mating was done in a 1:1 ratio and mating behaviour was observed on days 1 and 7. Similar procedure was repeated, normal saline, 12.5 and 25 mg/kg of the ethyl acetate, n-butanol and mother liquor fractions, respectively and sexual behavioral parameters were observed.Results: Crude extract and fractions of Waltheria indica significantly increased sexual behavioral parameters, with the n-butanol and mother liquor fractions being most active.Recommended applications/industries: The study showed that methanol root extract of Waltheria indica and its fractions have aphrodisiac activity with the n-butanol and residual methanol fractions being more active. These revelations further substantiates the traditional use of Waltheria indica for the management of infertility in male, thus such discovery can serve as a lead for the development of drugs for the management of sexual dysfunctions.
and Mgbere, O.O. 2020. The influence of graded levels of Cyathula prostrata (Linn.) Blume on semen quality characteristics of adult New Zealand white bucks. Translational Animal Science, 4:1134–1139.
Basiru, A. and Olayemi, F.O. 2014. Effects of aqueous extract of Waltheria indica leaves on blood profile of male albino rats. Journal of Medicinal Plant Research, 8(41): 1223-1229.
Chen, X. and Lee, T.J.F. 1995. Ginsenosides induced nitric oxide mediated relaxation of the rabbit carvenosum. British Journal of Pharmacology, 115(1): 15–18.
Corine, G.T., Pham, T.N. and Demougeot, C. 2015. Promise of plant-derived substances as inhibitors of arginase. Mini Reviews in Medicinal Chemistry, 15(10): 798–808.
Drewes, S.E., George, J. and Khan, F. 2003. Recent findings on natural products with erectile-dysfunction activity. Phytochemistry, 62: 1019–1025.
Enema, O.J., Umoh, U.F., Umoh, R.A. et al. 2018. Chemistry and Pharmacology of aphrodisiac plants: A review. Journal of Chemical and Pharmaceutical Research, 10(7): 70-98.
Evans, W. C. 2009. Trease and Evans Pharmacognosy. 16th edition Saunders Elsevier p. 350-450.
Ezebuiro, I., Obiandu, C., Tamuno-Opubo, A., Owhorji, B.I. and Obiandu, A.C. 2022. Improved reproductivefFunctions of male wistar rats administered with leaf extract of Cnidoscolus aconitifolius. Saudi Journal of Biomedical Research, 7(5): 184-189.
Fouche, G., Afolayan, A.J., Wintola, O.A., Thrombi, T.E. and Senabe, J. 2015. Effect of the aqueous extract of the aerial parts of Monsonia angustifolia E. Mey. Ex A. Rich., on the sexual behaviour of male Wistar rats. BMC Complementary and Alternative Medicine, 15: 343.
Gakunga, N.J., Mugisha, K., Owiny, D. and Paul, W. 2014. Effects of crude aqueous leaf extracts of Citropsis articulata and Mystroxylon aethiopicum on sex hormone levels in male albino rats. International Journal of Pharmaceutical Science Invention, 3(1): 5–17.
Gauthaman, K. and Adaikan, P.G. 2008. The hormonal effects of Tribulus terrestris and its role in the management of male erectile dysfunction- an evaluation using primates, rabbits and rats. Phytomedicine, 15: 44–54.
Gauthaman, K., Adaikan, P.G. and Prasda, R. N.V. 2002. Aphrodisiac properties of Tribulus terrestis extract (protodioscin) in normal and castrated rats. Life Science, 71: 1385–1396.
Ghosh, D., Mondal, S. and Ramakrishna, K. 2019. Acute and sub-acute (30-day) toxicity studies of Aegialitis rotundifolia Roxb., leaves extract in Wistar rats: safety assessment of a rare mangrove traditionally utilized as pain antidote. Clinical Phytoscience, 5(13): 1-16.
Gotep, J.G., Sabo, S.Y., Makama, S., Uguru, M.O., Okwuasaba F.K. 2021. Aphrodisiac activity of ethanol extract and fractions of Fadogia cienkowskii Schweinf. Rubiaceae roots in albino rats. Journal of Medicinal Plants Research, 15(2): 86-95.
Graham, J.G., Quinn, M.L., Fabricant, D.S. and Farnsworth, N.R. 2000. Plants used against cancer an extension of the work of Jonathan Hartwell. Journal of Ethnopharmacology, 73: 347–377.
Hernandez-Gonzalez M (2000) Prepubertal genital grooming: Penile erection in relation to sexual behavior of rats. Physiology and Behavior, 71(1-2): 51-56.
Hossain, M.A., Al-Hdhrami, S.S., Weli, A.M., Al-Riyami, Q. and Al-Sabahi, J.N. 2014. Isolation, fractionation and identification of chemical constituents from the leaves crude extracts of Mentha piperita L grown in Sultanate of Oman. Asian Pacific Journal of Tropical Biomedicine, 4(Suppl 1): S368-S372.
Jansen, O., Angenot, L and Tits, M. et al. 2010. In vitro antiplasmodial activity of ethnobotanically selected plants from Burkina Faso. Journal of Ethnopharmacology, 130: 143–150.
Kim, H.J., Woo, D.S., Lee, G and Kim, J.J. 1998. The relaxation effects of ginseng saponin in rabbit corporal smooth muscle, is it a nitric oxide donor. British Journal of Urology, 82: 744–748.
Kotta, S., Ansari, S.H. and Ali, J. 2013. Exploring scientifically proven herbal aphrodisiacs. Pharmacognosy Reviews, 7: 1-13.
Lorke, D. 1983. A new approach to practical acute toxicity testing. Archives of Toxicology 54(4):275–287.
Msiska, T., Mwakikunga, A., Tembo, D. and Lampiao, F. 2020. A phytochemical analysis and in vivo effects of an herbal aphrodisiac Newtonia hildebrandtii on male wistar rat reproductive system. Pharmacognosy Research, 12(3): 243-249.
Musa, Y., Musah, M., Yerima, H. and Erena, N.B. 2016. Study of Phytochemical and Antimicrobial Activities of Waltheria indica. Journal of Advance Research in Applied Science, 3(4): 30-39.
Njoga, U.J., Jaja, I.F., Onwuka, O.S et al. 2022. Reproductive effects of medicinal plant (Azadirachta indica) used as forage and for ethnoveterinary practices: New insights from animal models. Challenge, 13(40): 1-18.
Ofeimun, J.O. and Ayinde, B.A. 2017. Preliminary investigation of the aphrodisiac potential of the methanol extract and fractions of Rhaphiostylis beninensis Planch ex Benth (Icacinaceae) root on male rats. Journal of Science and Practice of Pharmacy, 4(1): 182-188.
Oigbochie, E.V., Omage, K. and Odiase, E.D. 2019. Aqueous root extract of Chrysophyllum albidum caused dose and duration dependent increases in some reproductive hormones and spermatogenic arrest in the testes of male wistar rats. Clinical Phytoscience, 5(3): 1-8.
Olajuyigbe, O.O., Babalola, A.E. and Afolayan, A.J. 2011. Antibacterial and phytochemical screening of crude ethanolic extracts of Waltheria indica Linn. African Journal of Microbiology Research, 5(22): 3760–3764.
Oluranti, A.C., Michael, U.O., Jane, U.C. and Ayembe, N.A. 2012. Ethno botanical studies of medicinal plants used in the management of Peptic ulcer disease in Sokoto State, North Western Nigeria. International Research Journal of Pharmacy and Pharmacology, 2(9): 225-230.
Padashetty, S.A. and Mishra, S.H. 2007. Aphrodisiac studies of Tricholepis glaberrima with supportive action from antioxidant enzymes. Pharmaceutical Biology, 45: 580-586.
Padhy, R.., Patro, S.K., Jena, M. and Dash, S.K. 2017. Toxicity evaluation of methanolic rhizome extracts obtained from Drynaria quercifolia (Linn.) J. Smith in experimental animals. The Saudi Journal of Life Sciences, 2(7): 255-258.
Pattij, T., De-Jong, T.R., Uitterdijk, A. et al. 2005. Individual differences in male rat ejaculatory behaviour: searching for models to study ejaculation disorders. European Journal of Neuroscience, 22(3): 724–734.
Ratnasooriya, W.D., Dharmasiri, M.G. 2000. Effects of Terminalia catappa seeds on sexual behavior and fertility of male rats. Asian Journal of Andrology, 2: 213–219.
Rowland. D.L. 1998. Penile sensitivity in men: a composite of recent findings. Urology, 52(6): 1101–1105.
Sanders, S.A., Graham, C.A., Bass, J.L and, Bancroft, J. 2001. A prospective study of the effects of oral contraceptives on sexuality and well-being and their relationship to discontinuation. Contraception, 64(1): 51-58.
Sharma, V., Thakur, M., Chauhan, N.S. and Dixit, V.K. 2010. Effects of petroleum ether extract of Anacyclus pyrethrum DC on sexual behaviour in male rats. Journal of Chinese Integrated Medicine, 8: 767–773.
Singh, R., Ali, A., Gupta, G., Semwal, A. and Jeyabalan, G. 2013. Some medicinal plants with aphrodisiac potential: A current status. Acute Disease, 2: 179 188.
Tajuddin, A.S., Latif, A. and Qasmi, I.A. 2004. Effect of 50% ethanolic extract of Syzygium aromaticum (L) Merr. & Perry.(Clove) on sexual behaviour of normal male rats. BMC Complementary & Alternative Medicine, 4: 17–24.
Tang, X., Olatunji, O.J., Zhou, Y. and Hou, X. 2017. In vitro and in vivo aphrodisiac properties of the seed extract from Allium tuberosum on corpus cavernosum smooth muscle relaxation and sexual behavior parameters in male Wistar rats. BMC Complementary and Alternative Medicine, 17: 510.
Yakubu, M.T, Akanji, M.A. and Oladiji, A.T. 2005. Aphrodisiac potentials of aqueous extract of Fadogia agrestis (Schweinf. Ex Heirn) stem in male albino rats. Asian Journal of Andrology, 7(4): 399–404.
Yakubu, M.T. and Afolayan, A.J. 2009. Effect of aqueous extract of Bulbine natalensis (Baker) stem on the sexual behaviour of male rats. International Journal of Andrology, 32: 629–636.
Yakubu, M.T., Awotunde, O.S., Ajiboye, T.O., Oladiji, A.T. and Akanji, M.A. 2011. Pro-sexual effects of aqueous extracts of Massularia acuminata root in male Wistar rats. Andrologia, 43: 334–340.
Zongo, F., Ribuot, C., Boumendjel, A. and Guissou, I. 2013. Botany, traditional uses, phytochemistry and pharmacology of Waltheria indica L. (syn.Waltheria americana): A review. Journal of Ethnopharmacology, 148: 14–26.
