Effect of milk thistle on the immune system, intestinal related variables, appearance and mortality of broilers contaminated with Aflatoxin B1
الموضوعات : مجله گیاهان داروییامید فانی مکی 1 , نظر افضلی 2 , آرش امیدی 3
1 - گروه علوم دامی، دانشکده کشاورزی، دانشگاه بیرجند، بیرجند، ایران
2 - گروه علوم دامی، دانشکده کشاورزی، دانشگاه بیرجند، بیرجند، ایران
3 - گروه مدیریت بهداشت دام، دانشکده دامپزشکی، دانشگاه شیراز، شیراز، ایران
الکلمات المفتاحية: immune system, broilers, Aflatoxin, Silybum marianum, Intestinal - related variables,
ملخص المقالة :
Background & Aim: Aflatoxin (AF) is the most important fungus that contaminates food and feed and can enter the food chain of humans. The aim of this study was to investigate the ability of thistle seeds (silybum marianum L.) to reduce the adverse effects of aflatoxin B1 (AFB1) on the immune system, variables related to the intestine, appearance and mortality of the broiler chickens.Experimental: In this experiment, a total of 216 male Ross 308 broiler chickens in a completely randomized factorial design 3 × 3 with nine treatments, four replications, and six chickens unit were grown on the ground for 35 days. Treatments was included three levels of AFB1 (0, 250, and 500 ppb) and three levels of milk thistle seed (0, 0.5 and 1%). Influenza and Newcastle diseases virus (IDV & NDV) titers in two steps (25 and 34 days), variables related to the intestine at the end of the experiment along with the weekly appearance and mortality of broiler chickens were evaluated.Results & Discussion: Analysis of IDV and NDV results in the day 34 showed a reduction in titers of them in birds receiving diets contaminated with 500 ppb AFB1 (p ≤ 0.05). Also, the length of the ileum and the total intestine at the end of the experimental period (day 35) indicated decrease in the group of birds received diets containing 500 ppb AFB1 (p ≤ 0.05). Chicks receiving diet containing 500 ppb of AFB1 had the most of feather abnormalities, aggressive behavior and minimal body size compared with other treatments. Mortality due to AFB1 consumption in various experimental treatments was not significant.Recommended applications-industries: Probably phitosomes of milk thistle seeds (MTS) reduce the mortality rate, nervous and aggressive behavior and increase the immunity of broilers against IDV & NDV in AFB1 exposure time.
Fani makki, O., Afzali, N., Omidi, A., Allahresani, A. and Frouzanmehr, M. 2013a. The Effect of Different Levels of Aflatoxin B1 on Intestinal Length, Blood Parameters and Immune System in Broiler Chickens. Journal of Veterinary Microbiology., 9(1): 73-79.
Fani makki, O., Afzali, N., Omidi, A. and Shibak, A. 2013b. Comparison of Aflatoxin B1 production by Aspergillus flavus and Aspergillus parasiticus under various conditions of temperature, light and pH. Armaghane-danesh Journal (YUMSJ)., 18(3), 210-218.
Fraschini, F., Demartini, G. and Esposti, D. 2002. Pharmacology of silymarin. Clinical Drug Investigation., 22(1): 51-65.
Giambrone, J. J., Diener, U. L., Davis, N. D., Panangala, V. S. and Hoerr, F. J. 1985 a. Effects of purified aflatoxin on broiler chickens. Poultry Science., 64(5): 852-858
Giambrone, J. J., Diener, U. L., Davis, N. D., Panangala, V. S. and Hoerr, F. J. 1985 b. Effects of aflatoxin on young turkeys and broiler chickens. Poultry Science., 64: 1678–1684.
Kalorey, D. R., Kurkure, N. V., Ramgaonkar, J. S., Sakhare, P. S., Warke, S. and Nigot, N. K. 2005. Effect of polyherbal feed supplement “Growell “during induced aflatoxicosis, ochratoxicosis and combined mycotoxicoses in broilers. Asian-Australasian Journal of Animal Science., 18: 375-383.
Kana, J. R., Teguia, A. and Tchoumboue, J. 2010. Effect of dietary plant charcoal from Canariumschweinfurthii Engl. and maize cob on aflatoxin B1toxicosis in broiler chickens . Advanced Animal Bioscience., 1: 462–463.
Kim, B. R., Kim, D. H., Park, R. K., Kwon, K. B., Ryu, D. G., Kim, Y. C., Kim, N.Y., Jeong, S., Kang, B. K. and Kim, K. S. 2000. Effect of an extract of the root of Scutellariabaicalensis and its flavonoids on
aflatoxin B1 oxidizing cytochrome P450 enzymes. Planta Medica., 67: 396–399.
Kuilman, M. E. M., Maas, R. F. M., Woutersen-van Nijnanten, F. M. and Fink-Gremmels, J. 2000. Inhibition of aflatoxin M1 production by bovine hepatocytes after intervention with oltipraz. Veterinary Quarterly., 22: 30–35.
Ledoux, D., Rottinghaus, G., Bermudez, A. and Alonso-Debolt, M. 1999. Efficacy of a hydrated sodium calcium aluminosilicate to ameliorate the toxic effects of aflatoxin in broiler chicks. Poultry Science., 78: 204–210.
Lee, S. E., Campbell, B. C., Molyneux, R. J., Hsegawa, S. and LeeH. S. 2001. Inhibitory effects of naturally occurring compounds on aflatoxin B1 biotransformation. Journal of Agricultural and Food Chemistry., 49: 5171–5177.
Miazzo, R., Rosa, C. A., Carvalho, E. D. Q., Magnoli, C., Chiacchiera, S. M., Palacio, G., Saenz, M., Kikot, A., Basaldella, E. and Dalcero, A. 2000. Efficacy of synthetic zeolite to reduce the toxicity of aflatoxin in broiler chicks. Poultry Science., 79(1): 1-6.
Miazzo, R., Peralta, M. F., Magnoli, C., Salvano, M., Ferrero, S., Chiacchiera, S. M., Carvalho, E. C., Rosa, C. A. and Dalcero, A. 2005. Efficacy of sodium bentonite as a detoxifier of broiler feed contaminated with aflatoxin and fumunisin. Poultry Science., 84: 1-8.
SAS Institute. 2001. SAS User’s Guide: Statistics, Version 6. SAS Institute, Inc., Cary, NC.
Tedesco, D., Steidler, S., Galletti, S., Tameni, M., Sonzogni, O., and Ravarotto, L. 2004. Efficacy of silymarin phospholipid complex in reducing the toxicity of aflatoxin B1 in broiler chicks. Poultry Science., 83(11): 1839-1843.
Yunus, A. W., Ghareeb, K., Abd-El-Fattah, A. A. M., Twaruzek, M. and Böhm, B. 2011. Gross intestinal adaptations in relation to broiler performance during a chronic aflatoxin exposure. Poultry Science., 90(8), 1683-1689.
Zahid, R. and Durrani, F.R. 2007. Biochemical, hematological, immunological and growth promotant role of feed added Milk Thistle (Silybum marianum) in broiler chicks. M.Sc (Hons) thesis submitted to NWFP Agric. Univ. Peshawar, Pakistan.
