Determination of alkaloids amount from Iranian Papaver bracteatum Lindl. by HPLC
الموضوعات : مجله گیاهان دارویی
زهره کدخدا
1
(دانشگاه آزاد اسلامی واحد شهر قدس، تهران، ایران؛)
سجاد صداقت
2
(دانشگاه آزاد اسلامی واحد شهر قدس، تهران، ایران؛)
شمس علی رضازاده
3
(پژوهشکده گیاهان دارویی جهاد دانشگاهی،کرج، ایران؛)
حسن علی نقدی بادی
4
(پژوهشکده گیاهان دارویی جهاد دانشگاهی،کرج، ایران؛)
رحیم تقی زاد فرید
5
(دانشگاه آزاد اسلامی واحد شهر قدس، تهران، ایران؛)
فرهاد حریری اکبری
6
(دانشگاه آزاد اسلامی واحد علوم و تحقیقات تهران، تهران، ایران؛)
الکلمات المفتاحية: alkaloid, Papaver bracteatum, Codeine, Thebaine,
ملخص المقالة :
Background & Aim: Papaver bracteatum Lindl. (Papaverace) is an important pharmaceutical plant which contains isoqinoline alkaloids. Isoqinoline alkaloids constitute one distinct group of secondary metabolites of the Papaverace family. Codeine and thebaine are important alkaloids of Papaver bracteatum which use widely due to its medicinal properties. Therefore it is necessary to determination the contents of these alkaloids in different parts of Iran. In order to determine the best natural habitat of Papaver bracteatum, containing the highest concentration of thebaine and codein different sample collected from different parts of Iran.Experimental: Using high performance liquid chromatography (HPLC) standard curve with the presence of two major alkaloids (thebaine and codeine) were drawn and alkaloids were extracted from capsule Papaver bracteatum and were determined by HPLC.Results & Discussion: Codeine contents in the sample of dry capsule Zanjan, Plour, Chalus road, Siah Bisheh, and Mazandaran were determined 0.04, 0.04, 0.03, 0.01 and 0.02% respectively. Thebaine content was determined 3.3, 0.61, 0.34, 0.31 and 0.06% respectively. Amounts of codeine for different regions ranged between 0.001 to 0.004% and, 0.06 to 3.3% for thebaine. The accessions collected from Zanjan has highest percentage of codeine and thebaine (3.34%)Industrial/Practical recommendation: The capsules collected from region of Zanjan in Iran have more contents of thebaine and codeine which possess many pharmaceutical applications, therefore it is highly recommended using accessions from the region of Zanjan to extract thebaine and codeine consumed in the pharmaceutical industry.
غفارزادگان، ر.، خدیوپارسی، پ.، خلیقی سیگارودی، ف.، پیرعلی همدانی، م.، کدخدا، ز.، رضازاده، ش.ع. 1389. بهینه سازی روش استخراج ماده اولیه دارویی هیوسین از گیاه بذرالبنج. فصلنامه گیاهان دارویی، 9: 87-95.
قاسمی، ع. 1388. گیاهان دارویی و معطر (شناخت واثرات آنها). انتشارات دانشگاه آزاد اسلامی. 560 صفحه.
Sharghi, N. and Lalezari, I. 1967. Papaver bracteatum lindl. a highly rich source of thebaine. Nature, 213: 1244.
Meshulam, H. and Lavie, D. 1980. The Alkaloidal Constituents of Papaver bracteatum Arya E. Phytochemistry, 19: 2633-2635.
Kuppers, F., Salemink, C., Bastart, M. and Paris, M. 1976. Alkaloids of Papaver bracteatum presence of codeine neopine and alpinine. Phytochemistry, 15: 444 - 445.
Lalezari, I., Nasseri, P. and Asgharian, R. 1974. Papaver bracteatum Lindl. population AryaII. Journal Parmaceutical Scince, 63: 1331.
Rostam, H., Maghssodi, A. and Fawzi, B. 1976. Direct spectrophotometer determination of thebainein arya II population capsule of Papaver bracteatum lindl. Journal of Pharmaceutical Sciences, 67: 32-34.
Schulz, H., Baranska, M., Quilitzsch, R. and Schütze, W. 2004. Determination of alkaloids in capsules, milk and ethanolic extracts of poppy (Papaver somniferum L.) by ATR-FT-IR and FT-Raman spectroscopy. Analyst, 129: 917-920.
Milo, J., Levy, A., Palevitch, D. and ladizinsky, G. 1988. High - performance liquid chromatographic analysis of the alkaloid spectrum i the roots and capsules of the species and hybrids of papaver section oxytona Journal of Chromatography A, 452: 563-570.
