Assessment of secondary metabolites: in vitro antiarthritic and antihemolytic potential of various extracts of garlic

الموضوعات : مجله گیاهان دارویی

سونیا جهری ¹ (Department of Life Sciences, ITM University, Gwalior)
نصیر خان ² (Department of Life Sciences, ITM University, Gwalior)
ساریتا تیواری ³ (Department of Life Sciences, ITM University, Gwalior)
لاویه شاکیاوار ⁴ (Department of Life Sciences, ITM University, Gwalior)

تاريخ الإرسال : 05 الثلاثاء , جمادى الأولى, 1436 تاريخ التأكيد : 08 الثلاثاء , شعبان, 1436 تاريخ الإصدار : 23 الجمعة , جمادى الثانية, 1437

الکلمات المفتاحية: In vitro, FTIR, Allium sativum, Secondary Metabolites, Antiarthritic, Antihemolytic,

ملخص المقالة :

Background & Aim:Allium sativum commonly known as garlic is an herb used over years to prevent from various ailments such as cardiovascular diseases, cognitive disorders and also enhances the immune system. The aim of the present study is to study the in vitro antiarthritic and antihemolytic potential of garlic in aqueous and methanolic extracts. Fourier transform infrared spectroscopy was performed to study the presence of functional groups. Experimental: The study involved detection of primary and secondary metabolites in allium sativum i.e garlic. In vitro antiarthritic and heat induced haemolytic assaywas evaluated so that it could be used against haemolytic diseases such as malaria, swine flu etc. FTIR spectra were performed to detect the presence of functional groups. Results: Natural products due to their immense bioactivity, finds applications against various diseases without having side effects. Recommended applications/industrie: This herb is very easily accessible and is edible it can be used as a pharmaceutical agent. Bioactive constituents can be isolated and explored for further activity.

المصادر:

Brown, JH., Mackey, HK. 1968. Inhibition of heat-induced denaturation of serum proteins by mixtures of non- steroidal anti-inflammatory agents and amino acids. proc soc exp biol med., 128:225-28

Chen, C., Pearson, AM., Gray, IJ. 1992. Effects of synthetic antioxidants (BHA, BHT and PG) on the mutagenicity of IQ-like   compounds. Food Chemistry., 43: 177-183.

Chiang, Y.H., Jen, L.N., Su, H.Y., Lii, C.K., Sheen, L.Y., Liu, C.T. 2006. Effects of garlic oil and two of its major organosulfur compounds, diallyl disulfide and diallyl trisulfide, on intestinal damage in rats injected with endotoxin. Toxicol Appl Pharmacol., 213:46–54.

Dixon, R.A., Xie, D.Y., Sharma, S.B. 2005. Proanthocyanidins- a final frontier in flavanoid research. New Phytol., 165: 9-28.

Eberhardt, T.L., Li, X., Shupe, T.F., Hse, C.Y. 2007. Chinese Tallow ree (Sapium Sebiferum) utilization: Characterization of extractives and cell-wall chemistry. Wood Fiber Sci., 39: 319–324.

Fisher, C.D., Augustine, L.M., Maher, J.M., Nelson, D.M., Slitt, AL., Klaassen, CD., Lehman-McKeeman, L.D., Cherrington, N.J. 2007. Induction of drug-metabolizing enzymes by garlic and allyl sulfide compounds via activation of car and CAR and   Nrf2. Drug Metab Dispos., 35: 995–1000.

Gupta, S.K., Gupta, A., Gupta, A.k., Pakash, D. 2103. In vitro anti-arthritic activity of callicarpa macrophylla flower.  Int. Res. J. Pharm., 4(3).

Hwang, I.G., Woo, K.S., Kim, D.J., Hong, J.T., Hwang, B.Y., Lee, Y.R., Jeong, H.S. 2007. Isolated and identification of an antioxidant substance from heated garlic (Allium sativum L.). Food Sci Biotechnol., 16: 963–966.

Kumar, S., Sharma, U.K., Sharma, A.k., and Pandey, A.K. 2012. Protective efficancy of Solanum xanthocarpum root extracts against free redical damage: Phytochemical analysis and antioxidant effect. Cell. Mol. Biol., 58(1): 174-181.

Lang,  A., Lahav, M., Sakhnini, E., Barshack, I., Fidder, H.H., Avidan, B., Bardan, E., Hershkoviz,  R., Bar-Meir S., Chowers Y.2004. Clin Nutr, Allicin inhibits spontaneous and TNF-alpha induced secretion of proinflammatory cytokines and chemokines from intestinal epithelial cells. 23:1199-1208. 

Murugavel P, Pari L. 2007. Effects of diallyl tetrasulfide on cadmium-induced oxidative damage in the liver of rats. Hum Exp Toxicol., 26: 527–534.

Neil A., Silagy C., 1994. Garlic: its cardiovascular-protective properties. Curr Opin Lipidol., 5: 989S–993S.

Osawa T. Japan: Japan Scientific Societies Press; 1994. Novel natural antioxidants for utilization in food and biological system; pp. 241–51.48

Okoli CO, Akah PA, Onuoha NJ, Okoye TC, Nwoye AC, Nworu CS. 2008. Acanthus montanus: An experimental evaluation of the antimicrobial, anti-inflammatory and immunological properties of a traditional remedy for furuncles. BMC Complement Altern Med. 8: 27.

Park EY., Ki SH., Ko MS., Kim CW., Lee MH., Lee YS., Kim SG.,2005. Garlic oil and DDB, comprised in a pharmaceutical composition for the treatment of patients with viral hepatitis, prevents acute liver injuries potentiated by glutathione deficiency in rats. Chem Biol Interact1., 55: 82–96.

Pari L, Murugavel P, Sitasawad SL, Kumar KS.2007. Cytoprotective and antioxidant role of diallyl tetrasulfide on cadmium induced renal injury: an in vivo and in vitro study. Life Sci., 80: 650–658.

Packer L, G. Rimbach, and F. Virgili. 1999. Antioxidant activity               and         biological properties of a procyanidin-rich extract from pine (Pinusmaritima) bark, pycnogel.             Free RadicBiol Med., 27: 704-24.

Plummer, D.T., 1987. An Introduction to Practical Biochemistry. Mc Graw Hill Education (India) Private Limited. Third Edition.  PP. 157-181

 Rahman, K., 2001.  Historical perspective on garlic and cardiovascular disease. J Nutr., 131: 977S–979S.

Rahman K. 2003. Garlic and aging: new insights into an old remedy. Ageing Res Rev., 2: 39–56.

Sabayan B., Foroughinia F.­­­­­, Chohedry A. 2007. A postulated role of garlic organosulfur compounds in prevention of valproic acid hepatotoxicity. Med Hypotheses., 68: 512-514.                    

Son EW., Mo SJ., Rhee DK., Pyo S.2006. Inhibition of ICAM-1 expression by garlic component, allicin, in gamma-irradiated human vascular endothelial cells via downregulation of the JNK signalling pathway. Int Immunopharmacol. 6: 1788-1795.                                                           

Shahidi F., Wanasundara P.K.J.P.D., 1992. Phenolic antioxidants. Food Sci Nutr., 32:67-103.

Tanaka S., Haruma K., Yoshihara M., Kajiyama G., Kira K., Amagase H., Chayama K. 2006.  Aged garlic extract has potential suppressive effect on colorectal adenomas in humans. J Nutr., 136: 821S–826S.

Venkataraman N.D., W Clement Atlee, T Purushoth Prabhu, , G Surya, R Kannan, I Sheik Nasar,  2013 , Evaluation of In-vitro Anti-Arthritic Potential of Aerial Parts of Ipomoeapes- caprae (L.) R.Brand Estabilishment of Its Mechanism of Action. Research Journal of Pharmaceutical, Biological and Chemical Sciences., 4(2): 1561-65

Walton   N. J. and D. E. Brown. 1999. Chemicals from plants: Perspectives on plant secondary product. London: Imperial College press.