Cytotoxic Effect of Hydroalcoholic Extract from Satureja bachtiarica Bunge on Hela Cancer Cells Line
الموضوعات : مجله گیاهان داروییآناهیتا اروند شوشتر 1 , حسین سازگار 2 , عبداله قاسمی پیربلوطی 3
1 - گروه بیولوژی، دانشکده علوم پایه، دانشگاه آزاد اسلامی واحد شهرکرد، شهرکرد، ایران
2 - گروه بیولوژی، دانشکده علوم پایه، دانشگاه آزاد اسلامی واحد شهرکرد، شهرکرد، ایران
3 - گروه گیاهان داروئی، دانشگاه آزاد اسلامی واحد شهرکرد، شهرکرد، ایران
الکلمات المفتاحية: MTT test, Hela, Satureja bachtiarica Bunge,
ملخص المقالة :
Background & Aim:Cervical cancer is the most common female genital cancer and the second most common cancer in women in the world. Since the herbal ingredients have been used for centuries to treat cancer, purpose of the present study was to determine the cytotoxic effect of hydroalcoholic extract from Satureja bachtiarica Bungeon Hela cancer cells line. Experimental: Hela cells line and natural fibroblast cells line were cultured in RPMI 1640 and DMEM Containing fetal bovine serum and antibiotics, respectively. The cells were exposed to different doses of hydroalcoholic extract of Satureja bachtiarica Bunge (0.156, 0.312, 0.625, 1.25, and 2.5 mg/ml) and were incubated for 24, 48 and 72 hr, respectively. After the incubation period, the modified MTT colorimetric test was used in order to determine cell toxicity of the extract. Results: The results of MTT test Showed that hydroalcoholic extract of Satureja bachtiarica Bunge has dose-dependent anti-cancer effect on Hela cancer cells, so that by increasing the concentration of extract and incubation in 72h, the most percentage of cell death was observed (p < 0.05). The extract of plant didn’t show any significant cytotoxicity on natural fibroblast cell line. Therefore, it seems that its compounds will be applied in treating cancer through more researches. Recommended applications/industries: Considering the low side effects of herbal medicines, especially antioxidants including Satureja bachtiarica Bunge can be used to treat cancer with drug therapy.
Abdullaev, F. 2001. Plant-derived agents cancer. Journal of Pharmacology. 15(3): 345-354.
Abdullaev, F.I. 2001. Cancer chemopreventive and tumorical properties of saffron (Crocus sativus L). Mexico city, Mexico: Laboratory of Experimented Oncology, National Institute of Pediatncs. Experimental Biology and Medicine. 227(1): 5-20.
Aggarwal, B.B., Sundaram, C., Malani, N., and Ichikawa, H. 2007. Curcumin: the Indian solid gold. Advances in Experimental Medicine and Biology. 595: 1-75.
Buchanan, R.L., and Shepherd, A.J. 1981. Inhibition of Aspergillus parasiticus by thymol. Journal of Food Sciences. 46: 976–977.
Castellsague, X., Arbyn, M., Bruni, L., Saraiya, M., Bray, F., and Ferlay, J. 2011. World wide burden of cervical cancer in 2008. Annals of Oncology. 22(12): 2675-2686.
DeVita VT Jr, Rosenberg SA. 2012. Two hundred years of cancer research. New England Journal of Medicine. 7; 366(23): 2207-2214.
Dadfar, Sh., Mirlohi, M., and Ghasemi Pirbalouti, A. 2013. Antimicrobial effect of Saturejaba chtiarica essential oil in ground beef contaminated with Pseudomonas aeruginosa during refrigerated period. Journal of Health Research. 1630-1637.
D’Angel, S., Morana, A., Salvatore, A., Zappia, V., and Galletti, P. 2009. Protective effect of polyphenols from Glycyrrhiza glabra against oxidative stress in Caco-2 cells. Journal of Medicinal Food. 12; 1326–1333.
Diaz-Cruz, E.S., Shapiro, C.L., and Brueggemeier, R.W. 2005. Cyclooxygenase inhibitors suppress aromatase expression and activity in breast cancer cells. Journal of Clincal Endocrinology & Metabolism. 90(5): 2563-2570.
Forouzandeh, F., Salimi, S., Naghsh, N., Zamani, N., and Jahani, S. 2014. Evaluation of anti-cancer effect of Peganum harmala L hydroalcoholicextract on human cervical carcinoma epithelial cell line. Journal of Shahrekord University of Medical Sciences. 16(4): 1-8.
Forouzandeh, S., Naghsh, N., Salimi, S., and Jahantigh, D. 2014. Cytotoxic Effect of Boswellia Serrata HydroalcoholicExtract on Human Cervical Carcinoma Epithelial Cell Line. Medical Laboratory Journal. 8(1): 6-12.
Galati, G., and Brien, P. 2004. Toxicity of flavonoids and other dietary phenolics: significance for their chemo preventiveand anticancer properties. free radical biology and medicine. 17(3): 287-303.
Giusepe, G., Abate, R., Liguori, G., Albano, L., and Angelilo, F. 2008. Human papilomavirus and vaccination, knowledge, attitudes, and behavioral intention in adolescents and young women in Italy. British Journal of Cancer. 9(2): 25-29.
Hamta, A., and Ghazaghi, S. 2014. The study of Thymus vulgaris Cytotoxicity effects on breast cancer cell's line. Quarterly Journal of Sabzevar University of Medical Sciences. 21(1): 122-130.
Jamshidi, M., Ahmadi-Ashtiani, H., Rezazadeh, S., Fathiazad, F., Mazandarani, M., and Khaki, A. 2010. Study on Phenolics and Antioxidant Activity of some Selected Plant of Mazandaran Province. Journal of Medicinal Plants. 2 (34): 177-182.
Keramati, K., Mehranpoor, M., Babakhani, A., Vaezi, G., and Habibi, F. 2012. Effect of Flunixin on Ovarian Cancer Induced by DMBA in Female Wistar Rats. Knowledge and Health. 7(1): 44-49.
Keramati, K., Sanaie, K., Babakhani, A., Rakhshan, M., Vaezi, G., and Haeri, A. 2011. Effect of Thymus Vulgaris hydro-alcoholic extraction on DMBA induced prostate cancer in Wistar rat. Research in Medicine. 35 (3): 135-140.
Kris-Etherton, P.M., Hecker, K.D., Bonanome, A., Coval, S.M., Binkoski, A.E., and Hilpert, K.F. 2002. Bioactive compounds in foods: their role in the prevention of cardiovascular disease and cancer. American Journal of Medicine. 113, 71–88.
Kumaran, A., and Karunakaran R.J. 2006. Antioxidant and free radical scavenging activity of an aqueous extract of Coleus aromaticus. Food Chemistry. 97: 109-114.
Moheghi, N., Tavakkol Afshari, J., and Brook, A. 2011. The Cytotoxic Effect of Zingiber officinale in Breast Cancer (MCF7) Cell Line. Journal of Gonabad University of Medical Sciences. 17(4): 28-33.
Mortazavian, M., Ghorbani Hesari, T. 2012. Effect of Hydro-Alcoholic Extracts of Viola Tricolor and its Fractions on Proliferation of Cervix Carcinoma Cells. Iranian Jounal of Obstetrics, Gyncology and Infertility. 15(22): 9-16.
Pokorny, J. 2007. Are Natural Antioxidants etter and Safer Than Synthetic Antioxidant Components. European journal of Lipid Science and Technology. 109:629-642.
Scherer, W.F., Syverton, J.T., and Gey, G.O. 1953. Studies on the propagation in vitro of poliomyelitis viruses. IV. Viral multiplication in a stable strain of human malignant epithelial cells (strain HeLa) derived from an epidermoid carcinoma of the cervix. Journal of Experimental Medicine. 97(5):695-710.
Sefidkon, F., Abbasi, K., Jamzad, Z., and Ahmadi, S. 2007. The effect of distillation methods and stage of plant growth on the essential oil content and composition of Satureja rechingeri Jamzad. Food Chemistry. 100(3): 1054-1058.
Sefidkon, F., and Jamzad, Z. 2005. Chemical composition of the essential oil of three Iranian Satureja species (S. mutica, S. macrantha and S. intermedia). Food Chemistry. 91(1): 1-4.
Sefidkon, F., and Jamzad, Z. 2004. Chemical composition of the essential oil of three Iranian Satureja species (S. mutica, S. macrantha and S. intermedia). Food Chemistry. 91:1–4.
Shukla, Y., and Singh, M. 2007. Cancer preventive properties of ginger: a brief review. Food and Chemical Toxicology. 45: 683-690.
Singh, B., and Lucci, A. 2002. Role of cycloxygenase-2 in breast cancer. Journal of Surgical Research. 108: 173-179.
Vaisy, A., Lotfinejad, Sh., and Zhian, F. 2013. Risk Factors for Cervical Cancer among Women Referred to Health Services Centers of Tehran University of Medical Sciences. Journal of Ardabil University of Medical Science. 13(3): 332- 341.
Wu, BJ, Kathir, K., Witting, P.K., Beck, K., Choy, K., and Li, C. 2006. Antioxidants protect from atherosclerosis by a heme oxygenase-1 pathway that is independent of free radical scavenging. Journal of Experimental Medicine. 203(4): 1117-1127.
