Genotyping of Lactoferrin and CXCR1 Genes in Guilan Native Cows and Its Association with Milk Somatic Cell Score
الموضوعات :
S.H. Hosseini Moghaddam
1
(Department of Animal Science, Faculty of Agricultural Science, University of Guilan, Rasht, Iran)
M. Ayatollahi
2
(Department of Animal Science, Faculty of Agricultural Science, University of Guilan, Rasht, Iran)
O. Ahadzadeh
3
(Department of Animal Science, Faculty of Agricultural Science, University of Guilan, Rasht, Iran)
S.Z. Mirhoseini
4
(Department of Animal Science, Faculty of Agricultural Science, University of Guilan, Rasht, Iran)
R. Khataminejad
5
(Department of Animal Science, Faculty of Agricultural Science, University of Guilan, Rasht, Iran)
H. Alaei
6
(Department of Genomics, North Region Branch of Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREEO), Rasht, Iran)
الکلمات المفتاحية: PCR-RFLP, <i>CXCR1</i> gene, lactoferrin gene, milk somatic cells, native cow,
ملخص المقالة :
Lactoferrin and CXCR1 genes are involved in immune responses related to mastitis infection. In this study, the polymorphism and association of lactoferrin (LF) and CXCR1 genes with milk somatic cell counts, as an indicator for mastitis detection, were investigated in Guilan native cow (Taleshi breed) using DNA blood samples of 100 cows from three different geographical zones (west, center, and east of Guilan province). The LF gene with a 301 bp fragment and CXCR1 with a fragment of 311 bp were amplified through PCR by using their specific primers. Then LF polymerase chain reaction (PCR) product was digested by EcoRI enzyme due to a single nucleotide polymorphism (SNP) at the related position (T>C) in intron 6 of LF and CXCR1 PCR product by BaeGI enzyme due to G > C SNP at position +735. Two alleles and three genotypes were observed for both genes in the studied populations. The observed genotypic frequencies of AA, AB, and BB were 52, 39, and 9% for LF and 67, 12, and 20% for CXCR1 locus, respectively. Three genotypes of LF locus were under Hardy-Weinberg equilibrium (P˃0.05) but it was not for CXCR1 locus. The mean of somatic cell counts was 138 × 103/mL, much lower than the reported data of pure-bred and crossbred cattle. Although there was no significant association (p <0.05%) between LF genotypes and somatic cell score (SCS), there was a tendency for association (p <0.1). The CXCR1c.+735 genotype had a significant association (p <0.05) with SCS. Sampling from different regions did not show a significant effect on SCS. The fix effects including lactation month, age, and lactation number had also no significant effect on SCS of the studied native cow.
Beecher C., Daly M., Ross R.P., Flynn J., McCarthy T.V. and Giblin L. (2012). Characterization of the bovine innate immune response in milk somatic cells following intramammary infection with Streptococcus dysgalactiae subspecies dysgalactiae. J. Dairy Sci. 95, 5720-5729.
Chaneton L., Tirante L., Maito J., Chaves J. and Bussmann L.E. (2008). Relationship between milk lactoferrin and etiological agent in the mastitic bovine mammary gland. J. Dairy Sci. 91, 1865-1873.
Chegini A., Ghavi Hossein-Zadeh N., Hosseini Moghaddam S.H. and Shadparvar A.A. (2018). Genetic correlation estimates between milk production traits, mastitis and different measures of somatic cells in Holstein cows. Anim. Prod. Sci. 59, 1031-1038.
Daly M., Ross P., Giblin L. and Buckley F. (2006). Polymorphisms within the lactoferrin gene promoter in various cattle breeds. Anim. Biotechnol. 17, 33-42.
De Haas E., Ouweltjes W., ten Napel J., Windig J.J. and de Jong G. (2008). Alternative somatic cell count traits as mastitis indicators for genetic selection. J. Dairy Sci. 91, 2501-2511.
Galvao K.N., Pighetti G.M., Cheong S.H., Nydam D.V. and Gilbert R.O. (2011). Association between interleukin-8 receptor-alpha (CXCR1) polymorphism and disease incidence, production, reproduction, and survival in Holstein cows. J. Dairy Sci. 94, 2083-2091.
Green M.J., Green L.E., Schukken Y.H., Bradley A.J., Peeler E.J., Barkema H.W., De Haas Y., Collis V.J. and Medley G.F. (2004). Somatic cell count distributions during lactation predict clinical mastitis. J. Dairy Sci. 87, 1256-1264.
Hagiwara S., Kawai K., Anri A. and Nagahata H. (2003). Lactoferrin concentrations in milk from normal and subclinical mastitic cows. J. Vet. Med. Sci.65, 319-323.
Hemati Doust V., Rahimi-Mianji G. and Farhadi A. (2013). Association between bovine lactoferrin gene variant and somatic cell count in milk based on Ecor1 restriction site. Iranian J. Vet. Res. 15, 62-65.
Hirvonen J., Eklund K. and Teppo A.M. (1999). Acute phase response in dairy cows with experimentally induced mastitis. Acta Vet. Scandinavica. 40, 35-46.
Leyva-Baca I., Schenkel F., Martin J. and Karrow N.A. (2008). Polymorphisms in the 5 ' upstream region of the CXCR1 chemokine receptor gene, and their association with somatic cell score in Holstein cattle in Canada. J. Dairy Sci. 91, 407- 417.
Miller S.A., Dykes D.D. and polesky H.F. (1988). A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Res. 16, 1215-1222.
O'Brien B., Berry D., Kelly P., Meaney W.J. and O'Callaghan E.J. (2009). A study of the somatic cell count (SCC) of Irish milk from herd management and environmental perspectives. Project Number 5399, Teagasc.
O’Halloran F., Berry D.P., Bahar B., Howard D.J., Sweeney T. and Giblin L. (2010). Polymorphisms in the bovine lactoferrin promoter are associated with reproductive performance and somatic cell count. J. Dairy Sci. 93, 1253-1259.
Pawlik A., Sender G. and Korwin-Kossakowska A. (2009). Bovine lactoferrin gene polymorphism and expression in relation to mastitis resistance-a review. Anim. Sci. Pap. Rep. 27, 263-271.
Pfaffl M.W., Wittmann S.L., Meyer H.H.D. and Bruckmaier R.M. (2003). Gene expression of immunologically important factors in blood cells, milk cells and mammary tissue of cows. J. Dairy Sci. 86, 538-545.
Rekik B., Ajili N., Hani H.B., BenGara A. and Rouissi H. (2008). Effect of somatic cell count on milk and protein yields and female fertility in Tunisian Holsteins cows. Livest. Sci. 106, 309-317.
SAS Institute. (2004). SAS®/STAT Software, Release 9.4. SAS Institute, Inc., Cary, NC. USA.
Sender G., Pawlik A., Korwin-Kossakowska A., Galal Abdel Hameid K., Sobczyńska M., Oprzadek J. and Prusak B. (2010). Association of the bovine lactoferrin polymorphism with occurrence of mastitis. Milchwissenschaft. 65, 242-245.
Seyfert H.M., Tuckoricz A., Interthal H., Koczan D. and Hobom G. (1994). Structure of the bovine lactoferrin-encoding gene and its promoter. Gene. 143, 265-269.
Shem M.N., Mosha F.A., Machangu R., Kambarage D. and Fujihara T. (2002). Bovine mastitis in Zebu and crossbred cattle under the extensive management system in Tanzania. Asian-australasian J. Anim. Sci. 15, 751-756.
Swanson K.M., Stelwagen K., Dobson J. and Henderson H.V. (2009). Transcriptome profiling of Streptococcus uberis induced mastitis reveals fundamental differences between immune gene expression in the mammary gland and in a primary cell culture model. J. Dairy Sci. 92, 117-129.
Tančin V. (2013). Somatic cell counts in milk of dairy cows under practical conditions. Slovak J. Anim. Sci. 46, 31-34.
Tavakolian J. (2000). An introduction to genetic resources of native farm animals in Iran. Animal Science Genetic Research Institute Press, Tehran, Iran.
Ward P.P., Paz E. and Conneely O.M. (2005). Multifunctional roles of lactoferrin: A critical overview. Cell. Mol. Life Sci. 62, 2540-2548.
Wojdak Maksymiec K., Kmiec M. and Ziemak J. (2006). Associations between bovine lactoferrin gene polymorphism and somatic cell count in milk. Vet. Med. 51, 14-20.
Youngerman S.M., Saxton A.M. and Pighetti G.M. (2004). Novel single nucleotide polymorphisms and haplotypes within the bovine CXCR2 gene. Immunogenetics. 56, 355-359.
Zhang C.L., Wang Y.Y., Chen H., Fang X.T. and Gu C.W. (2012). The chemokine receptor 1 gene polymorphism and its association with somatic cell score and milk production traits in dairy cattle. Anim. Sci. Pap. Rep. 30, 25-33.
Zhang J.X., Zhang S.F., Wang T.D., Guo X.J. and Hu R.L. (2007). Mammary gland expression of antibacterial peptide genes to inhibit bacterial pathogens causing mastitis. J. Dairy Sci. 90, 5218-5225.
Zhao C.H., He G.M., Wang Y.L. and Zhang Z. (2008). Polymorphism analysis of the promoter of cow Lactoferrin gene with PCR-RFLP and its correlation with subclinical mastitis. Acta Agric. Slovenica. 92, 185-187.
