Administration of Different Levels of Arginine and Lysine Coupled with Copper for Change the Copper Concentration of Milk in the Lactating Zandi’s Ewes
الموضوعات :
ا. پردل
1
(Department of Animal Physiology, Faculty of Life Science and Biotechnology, Shahid Beheshti University, Tehran, Iran)
ه. خزعلی
2
(Department of Animal Physiology, Faculty of Life Science and Biotechnology, Shahid Beheshti University, Tehran, Iran)
ح. رکنی
3
(Department of Education, Institute of Applied Scientific Education of Jihad-e-Agriculture, Tehran, Iran)
ع. حسینی
4
(Department of Animal Physiology, Faculty of Life Science and Biotechnology, Shahid Beheshti University, Tehran, Iran)
الکلمات المفتاحية: Copper, Lysine, arginine, ewe milk, lactation stage,
ملخص المقالة :
Copper (Cu) plays an essential role in the human and animal body system. One of the most important sources of Cu is milk. The purpose of this study was to determine the effect of different doses of injection of inorganic Cu and organic Cu as Cu-arginine and Cu-lysine on Cu concentrations of milk in the lactating Zandi’s ewes. This experimental research involved 54 lactating Zandi’s ewes collected from the department of State Organization Agriculture of Tehran province, Iran. They were allocated into three main groups included: group 1) which received inorganic form of Cu as copper chloride, groups 2 and 3) which received organic Cu as Cu-arginine or Cu-lysine (25, 50 and 100 mg kg-1 of body weight (BW)) in the chelate form, respectively. The milk samples were taken 30 min before and 6 hours after intravenous injection of Cu preparations. The Cu concentration in milk was measured using the flame atomic absorption spectrometric method. There was no significant difference among the groups in milk’s Cu concentration before administration of either inorganic or organic Cu. Data analysis showed that the administration of inorganic Cu caused a significant increase (P<0.01) of Cu concentration in the milk in a dose dependent manner. Furthermore, the administration of Cu-arginine or Cu-lysine in chelate form caused a significant increase (P<0.01) in milk Cu concentration compared to the inorganic Cu. In conclusion, administration of different forms of Cu influenced the Cu concentration in the milk. The organic forms of Cu led to a significant increase in milk Cu concentration in a dose dependent manner compared to the inorganic form of Cu.
AFRC. (1995). Energy and Protein Requirements of Ruminants. CAB International, Wallingford, UK.
Ashmead H.D. (2012). Amino Acid Chelation in Human and Animal Nutrition. CRC Press, New York.
Fantuz F., Ferraro S., Todini L., Mariani P., Piloni R. and Salimei E. (2013). Essential trace elements in milk and blood serum of lactating donkeys as affected by lactation stage and dietary supplementation with trace elements. Animal. 7, 1893-1899.
Felix T.L., Weiss W.P., Fluharty F.L. and Loerch S.C. (2012). Effects of copper supplementation on feedlot performance, carcass characteristics, and rumen sulfur metabolism of growing cattle fed diets containing 60% dried distillers grains. J. Anim. Sci. 90, 2710-2716.
Freestone D., Cater M.A., Ackland M.L., Paterson D., Howard D.L., Jonge M.D. and Michalczyk A. (2014). Copper and lactational hormones influence the CTR1 copper transporter in PMC42-LA mammary epithelial cell culture models. J. Nutr. Biochem. 25, 377-87.
Hackbart K.S., Ferreira R.M., Dietsche A.A., Socha M.T., Shaver R.D., Wiltbank M.C. and Fricke P.M. (2010). Effect of dietary organic zinc, manganese, copper, and cobalt supplementation on milk production, follicular growth, embryo quality, and tissue mineral concentrations in dairy cows. J. Anim. Sci. 88, 3856-3870.
Hatfield P.G., Swenson C.K., Kott R.W., Ansotegui R.P., Roth N.J. and Robinson B.L. (2001). Zinc and copper status in ewes supplemented with sulfate- and amino acid-complexed forms of zinc and copper. J. Anim. Sci. 79, 261-266.
Kelleher S.L. and Lonnerdal B. (2006). Mammary gland copper transport is stimulated by prolactin through alterations in CTR1 and ATP7A localization. Am. J. Physiol. Regul. Integr. Comp. Physiol. 291, 1181-1191.
Kirchgessner M. and Weigand M. (1982). Optimal zinc requirements of lactating dairy cows based on various dose-response relationships. Arch. Tierenahr. 32, 569-578.
Lee J., Prohaska J.R., Dagenais S.L., Glover T.W. and Thiele D.J. (2000). Isolation of a murine copper transporter gene, tissue specific expression and functional complementation of a yeast copper transport mutant. Gene. 254, 87-96.
Lee J., Prohaska J.R. and Thiele D.J. (2001). Essential role for mammalian copper transporter CTR1 in copper homeostasis and embryonic development. Proc. Natl. Acad. Sci. USA. 98, 6842-6847.
Leone A. and Mercer F.B.J. (1999). Copper Transport and Its Disorders: Molecular and Cellular Aspects. Kluwer Academic, Plenum Publishers, New York.
Lönnerdal B. (2007). Trace element transport in the mammary gland. Ann. Rev. Nutr. 27, 165-177.
Michalczyk A.A., Reiger J., Allen K.J., Mercer J.F. and Ackland M.L. (2000). Defective localization of the Wilson disease protein (ATP7B) in the mammary gland of the toxic milk mouse and the effects of copper supplementation. Biochem. J. 352, 565-571.
Miller W.J. (1975). New concepts and developments in metabolism and homeostasis of inorganic elements in dairy cattle: A review. J. Dairy. Sci. 58, 1549-1560.
Moreno-Rojas R., Amaro-Lopez M.A. and Zurera-Cosano G. (1993). Micronutrients in natural cow, ewe and goat milk. Int. J. Food Sci. Nutr. 44, 37-46.
Nemec L.M., Richards J.D., Atwell C.A., Diaz D.E., Zanton G.I. and Gressley T.F. (2012). Immune responses in lactating Holstein cows supplemented with Cu, Mn, and Zn as sulfates or methionine hydroxy analogue chelates. J. Dairy. Sci. 95, 4568-4577.
Nockels C.F., DeBonis J. and Torrent J. (1993). Stress induction affects copper and zinc balance in calves fed organic and inorganic copper and zinc sources. J. Anim. Sci. 71, 2539-2545.
Olkowski A.A., Gooneratne S.R. and Christensen D.A. (1990). Effects of diets of high sulphur content and varied concentrations of copper, molybdenum and thiamine on in vitro phagocytic and candidacidal activity of neutrophils in sheep. Res. Vet. Sci. 48, 82-86.
Pal D.T., Gowda N.K., Prasad C.S., Amarnath R., Bharadwaj U., Suresh Babu G. and Sampath K.T. (2010). Effect of copper- and zinc-methionine supplementation on bioavailability, mineral status and tissue concentrations of copper and zinc in ewes. J. Trace Elem. Med. Biol. 24, 89-94.
Pechova A., Misurova L., Pavlata L. and Dvorak R. (2009). The influence of supplementation of different forms of zinc in goats on the zinc concentration in blood plasma and milk. Biol. Trace Elem. Res. 132, 112-121.
Sobhanirad S., Carlson D. and Bahari Kashani R. (2010). Effect of zinc methionine or zinc sulfate supplementation on milk production and composition of milk in lactating dairy cows. Biol. Trace Elem. Res. 136, 48-54.
SPSS Inc. (2011). Statistical Package for Social Sciences Study. SPSS for Windows, Version 20. Chicago SPSS Inc.
Wang F., Li S.L., Xin J., Wang Y.J., Cao Z.J., Guo F.C. and Wang Y.M. (2012). Effects of methionine hydroxy copper supplementation on lactation performance, nutrient digestibility, and blood biochemical parameters in lactating cows. J. Dairy. Sci. 95, 5813-5820.
