Hormonal Profile of Ovarian Follicular Fluid and Blood Plasma during Different Stages of Estrous Cycle in Holstein Cattle
الموضوعات :ص. طباطبائی 1 , م. اصغری مقدم 2 , م. مموئی 3 , K. میرزاده 4 , ع. آقایی 5
1 - Department of Animal Science, Ramin Agriculture and Natural Resources University, Mollasani, Ahwaz, Iran
2 - Department of Animal Science, Ramin Agriculture and Natural Resources University, Mollasani, Ahwaz, Iran
3 - Department of Animal Science, Ramin Agriculture and Natural Resources University, Mollasani, Ahwaz, Iran
4 - Department of Animal Science, Ramin Agriculture and Natural Resources University, Mollasani, Ahwaz, Iran
5 - Department of Poultry Science, Ramin Agriculture and Natural Resources University, Mollasani, Ahwaz, Iran
الکلمات المفتاحية: progesterone, bovine, estradiol-17-β, estrous cycle, gonadotropins,
ملخص المقالة :
The aim of this study was to evaluate the concentrations of reproductive hormones in blood plasma and in follicular fluids of different sized follicles during various stages of the estrous cycle in Holstein cattle. Jugular blood samples from 42 adult Holstein cattle were collected immediately after the slaughter. Antral fluids from small (4-5 mm), medium (6-9 mm) and large (10-20 mm) follicles were collected and the stages of estrous cycle were recorded. The gonadotropin and steroid concentrations in blood plasma and steroid levels in follicular fluid were measured. Estradiol-17-ßconcentrations in antral fluids of small follicles in early diestrous were significantly(P<0.05) higher than the concentration of that in the follicular fluids from small antral follicles in other stages of the estrous cycle.Concentrations of estradiol-17-ßin follicular fluids from medium antral follicles in metestrous were significantly (P<0.05) higher than that of those in pro-estrous and estrous phase of the estrous cycle. There were no significant differences in the follicular fluid concentrations of estradiol-17-ßfrom the large follicles among the various stages of estrous cycle (P>0.05). The variation in progesterone concentration within the follicles of various size during different phases of estrous cycle was not significant (P>0.05). The plasma concentrations of FSH, LH and estradiol-17-ßin proestrous and estrous were higher than the other stages of the cycle (P<0.05). The plasma progesterone concentration in late diestrous was higher than in metestrous, proestrous and during estrous (P<0.05).
Ali A., Abdel Razek A., Abdel Ghaffar S. and Glatzel P.S. (2003). Ovarian follicular dynamics in buffalo cows (Bubalus bubalis). Reprod. Domest. Anim. 38, 214-218.
Alvarez P., Spicer L.J., Chase C.C., Payton M.E., Hamilton T.D., Stewart R.E., Hammond A.C., Olson T.A. and Wettemann R.P. (2000). Ovarian and endocrine characteristics during an estrous cycle in Angus, Brahman, and Senepol cows in a subtropical environment.J. Anim. Sci. 78, 1291-1302.
Azawi O.I., Ali A.J. and Noaman U.T. (2009). A study on the ovarian follicular dynamic in Iraqi Northern Buffaloes. Trop. Anim. Health Prod. 41, 79-83.
Baby T.E. and Bartlewski P.M. (2011). Progesterone as the driving regulatory force behind serum follicle-stimulating hormone (FSH) concentrations and antral follicular growth in cyclic ewes. Reprod. Fertil. Dev. 23, 303-310.
Bridges T.S., Davidson T.R., Chamberlain C.S., Geisert R.D. and Spicer L.J. (2002). Spicer changes in follicular fluid steroids, insulin like growth factors (IGF) and IGF-binding protein concentration and proteolytic activity during equine follicular development.J. Anim. Sci. 80, 179-190.
Christensen D.S., Hopwood M.L. and Wiltbank J.N. (1974). Levels of hormones in the serum of cycling beef cows. J. Anim. Sci. 38, 577-583.
Cox R.I., Mattner P.E. and Thorburn G.D. (1971). Changes in ovarian secretion of oestradiol-17-ß around oestrous in sheep. J. Endocrinol. 49, 345-346.
Eissa H.M. (1996). Concentrations of steroids and biochemical constituents in follicular fluid of buffalo cows during different stages of the oestrous cycle. Br. Vet. J. 152, 573-581.
Evans A.C.O. (2003). Characteristics of ovarian follicle development in domestic animals. Reprod. Dom. Anim. 38, 240-246.
Ginther O.J., Beg M.A., Donadeu F.X. and Bergfelt D.R. (2003). Mechanism of follicle deviation in monovular farm species. Anim. Reprod. Sci. 78, 239-257.
Ginther O.J., Bergfelt D.R., Kulick L.J. and Kot K. (2000). Selection of the dominant follicle in cattle: role of estradiol. Biol. Reprod. 83, 383-389.
Glister C., Tannetta D.S., Groome N.P. and Knight P.G. (2001). Interactions between follicle stimulating hormone and growth factors in modulating secretion of steroids and inhibin-related peptides by nonluteinized bovine granulose cells. Biol. Reprod. 65, 1020-1028.
Gordon I. (2003). Laboratory Production of Cattle Embryos. CABI., London, UK.
Henderson K.M., McNeilly A.S. and Swanston I.A. (1982). Gonadotrophin and steroid concentrations in bovine follicular fluid and their relationship to follicle size. J. Reprod. Fertil. 65, 467-473.
Ireland J.J., Coulson P.B. and Murphree R.L. (1979). Follicular development during four stages of the estrous cycle of beef cattle. J. Anim. Sci. 49, 1261-1269.
Kruip T.A. and Dieleman S.J. (1985). Steroid hormone concentrations in the fluid of bovine follicles relative to size, quality and stage of the oestrus cycle. Theriogenology. 24, 395-408.
Maurasse C., Matton P. and Dufour J.J. (1985). Ovarian follicular populations at two stages of an estrous cycle in heifers given high energy diets. J. Anim. Sci. 61, 1194-1200.
MedanM.S., Watanabe G., Sasaki K., Groome N.P., Sharawy S. and Taya K. (2005). Follicular and hormonal dynamics during the estrous cycle in goats. J. Reprod. Dev. 51, 455-463.
Medan M.S., Watanabe G., Sasaki K., Sharawy S., Groome N.P. and Taya K. (2003). Ovarian dynamics and their associations with peripheral concentrations of gonadotropins, ovarian steroids and inhibin during the estrous cycle in goats. Biol. Reprod. 69, 57-63.
Menchaca A. and Rubianes E. (2002). Relation between progesterone concentrations during the early luteal phase and follicular dynamics in goat. Theriogenology. 57, 1411-1419.
Mihm M. and Bleach E.C.L. (2003). Endocrine regulation of ovarian antral follicle development in cattle. Anim. Reprod. Sci. 78, 217-237.
Mondal S., Prakash B.S. and Palta P. (2007).Endocrine aspects of oestrous cycle in buffaloes (Bubalus bubalis): an overview. Asian-australs J. Anim. Sci. 20, 124-131.
Naik B.R., Siva Kumar A.V.N., Bramhaiah K.V., Ravi A. and Praveen Chakravarthi V. (2013). Estrogen and progesterone hormone levels in Punganur cattle. IOSR. J. Agric. Vet. Sci. 2, 50-53.
Nishimoto H., Hamano S., Hill G.A., Miyamoto A. and Tetsuka M. (2009). Classification of bovine follicles based on the concentrations of steroids, glucose and lactate in follicular fluid and the status of accompanying follicles. J. Reprod. Dev. 55, 219-224.
Opara M.N., Okoli C.I., Herbert U. and Adeyemo O. (2006). Ovarian morphology and estradiol-17-ßconcentrations in serum and follicular fluid of slaughtered zebu cattlein Ibadan, Nigeria.Vet. Arhiv. 76, 403-411.
Pang X.S., Wang Z.Y., Zhu T.G., Yin D.Z., Zhang Y.L., Meng L. and Wang F. (2010). Concentrations of progesterone and estradiol in peripheral plasma during the estrous cycle and after ovariectomy in Huanghuai goats of high or poor prolificacy. Asian-australs J. Anim. Sci. 23, 188-196.
Pattison M.L., Chen C.L., Kelley S.T. and Brandt G.W. (1974). Luteinizing hormone and estradiol in peripheral blood of mares during estrous cycle. Biol. Reprod. 11, 245-250.
Rodriguez R.E. and Wise M.E. (1989). Ontogeny of pulsatile secretion of gonadotropin- releasing hormone in the bull calf during infantile and pubertal development. Endocrinology. 124, 248-256.
Rosales Torres A.M., Sanchez A.G. and Aguilar C.G. (2012). Follicular development in domestic ruminants. Trop. Subtrop. Agroeco. 15, 147-160.
Sakhong D., Vongpralub T., Katawatin S. and Sirisathien S. (2011). Ovarian follicular patterns and hormone profile in Thai native cattle (Bos indicus). Thai. J. Vet. Med. 41, 439-447.
Souza C.J.H., Campbell B.K. and Baird D.T. (1997). Follicular dynamics and ovarian steroid secretion in sheep during the follicular and early luteal phases of the estrous cycle. Biol. Reprod. 56, 483-488.
Souza C.J.H., Campbell B.K. and Baird D.T. (1998). Follicular waves and concentrations of steroids and inhibin A in ovarian venous blood during the luteal phase of the oestrous cycle in ewes with an ovarian autotransplant. J. Endocrinol. 156, 563-572.
Sunderland S.J., Crowe M.A., Boland M.P., Roche J.F. and Ireland J.J. (1994). Selection, dominance and atresia of follicles during the oestrous cycle of heifers. J. Reprod. Fertil. 101, 547-555.
Vizcarra J.A., Wettemann R.P., Braden T.D., Turzillo A.M. and Nett T.M. (1997). Effect of gonadotropin releasing hormone (GnRH) pulse frequency on serum and pituitary concentrations of luteinizing hormone and follicle-stimulating hormone, GnRH receptors, and messenger ribonucleic acid for gonadotropin subunits in cows. Endocrinolgy. 138, 594-601.
Wise T. (1987). Biochemical analysis of bovine follicular fluid: Albumin, total protein, lysosomal enzymes, ions, steroids and ascorbic acid content in relation to follicular size, rank, atresia classification and day of estrous cycle. J. Anim. Sci. 64, 1153-1169.
Zieba D.A., Murawski M. and Wierzchos E. (2001). Pattern of follicular development during the oestrous cycle of prolific Olkuska sheep. Arch. Tierz. Dummerstorf. 44, 203-212.
