Allele Frequency of c.486A>G Polymorphism of the AA-NAT Gene in Iranian Indigenous and Exotic Sheep Populations
الموضوعات :
S. Emanizadeh
1
(Department of Animal Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran)
H. Alnajm
2
(Department of Animal Production Techniques, Al-Musaib Technical College, Al-Furat Al-Awsat Technical University, Babylon, Iraq)
A. Javanmard
3
(Department of Animal Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran)
J. Shoja
4
(Department of Animal Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran)
A. Rafat
5
(Department of Animal Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran)
الکلمات المفتاحية: PCR-RFLP, melatonin, <i>AA-NAT</i> gene, estrus non-breeding season,
ملخص المقالة :
Typically, lambing percentage is classified as a composite trait and is crucial to profitability in domestic sheep farming. In breeding programs, out-of-season reproduction of sheep is an important tool because seasonal reproduction limits productivity and flexibility. Understanding the complexities of genetic aspects of the none- seasonal reproduction has received significant critical attention in the literature. In this puzzle, the arylalkylamine-N-acetyltransferase (AA-NAT) is a rate-limiting enzyme of the melatonin synthesis pathway and is highlighted as a candidate gene that is responsible for melatonin synthesis and is thus directly associated with out-of-season reproduction in sheep. With this scenario research, we aimed to examine the allele frequency of the polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) polymorphism in the AA-NAT gene in 11 native and exotic sheep populations. A total of 220 blood samples were taken from 11 breeds of sheep, including the exotic breeds Romanov (ROMV) and [Awassi (AWAS), Arabi (ARAB), Naaimi (NAIM) Iraqi native sheep] and [Ghezel (GHZL), Makui (MAKU)], Kurdi (KURD), Baluchi (BALCH), Afshari (AFSR) Iranian native sheep] and two Afshari-Booroola and Romanov-Ghezel F1 cross). Here, we describe the Smal-RFLP genotypes and allele frequency patterns of the (c.486A>G) casual single nucleotide polymorphism (SNP) in the exon of AA-NAT within and between the examined sheep breeds. In addition, PCR sequencing methods were used to double-check A/G and confirmation of PCR-RFLP results. This mutation changed the Arg > Gly structure from helix to helix-effective in improving non-seasonal reproduction. Interestingly, the observed variation of G allele ranged from 0.1 to 0.43 in all study breeds. ROMV is a candidate for non-seasonal sheep breeds and its cross expresses the highest G allele frequency among other breeds studied. The frequency of the AA-NAT genotype was significantly different between breeds in this study. Therefore, the exotic 486A>G mutation created a useful mirror to identify genomics aspects of seasonal/non-seasonal reproduction in sheep.
Aboul-Ela M.B. and Chemineau P. (1990). Seasonality of reproductive activity in native sheep and goat breeds and their crosses with introduced breeds. Pp. 47 in Small Rumin. Res. Dev. Cairo, Egypt.
Addin G.N., Mohammed T.R. and Al-Anbari N.N. (2016). Polymorphism of AA-NAT gene and its relationship with the productive and reproductive performance in Turkish Awassi ewes. Anbar J. Agric. Sci. 14, 246-255.
Asadi-Fozi M., Bradford H.L. and Notter D.R. (2020). Direct and correlated responses to selection for autumn lambing in sheep. Genet. Sel. Evol. 52, 56-65.
Bartlewski P.M., Baby T.E. and Giffin J.L. (2011). Reproductive cycles in sheep. Anim. Reprod. Sci. 124, 259-268.
Bister J.L., Noel B., Perrad B., Mandiki S.N.M., Mbayahaga J. and Paquay R. (1999). Control of ovarian follicle activity in the ewe. Domest. Anim. Endocrinol. 17, 315-328.
Bozhilova M., Dimitrova I. and Petrov N. (2019). Investigation of polymorphisms on ABCG2 and AA-NAT genes in different sheep breeds in Bulgaria. Bulgarian J. Agric. Sci. 25, 49-53.
Cameron J.J., Stinson D.A., Gaetz R. and Balchen S. (2010). Acceptance is in the eye of the beholder: Self-esteem and motivated perceptions of acceptance from the opposite sex. J. Pers. Soc. Psychol. 99, 513-521.
Chattoraj A., Liu T.C., Zhang L.S., Huang Z.P. and Borjigin j. (2009). Melatonin formation in mammals: In vivo perspectives. Rev. Endocr. Metab. Disord. 10, 237-243.
Cipolla-Neto J. and Amaral F.G.D. (2018). Melatonin as a hormone: New physiological and clinical insights. Endocr. Rev. 39, 990-1028.
Coon S.L., Mazuruk K., Bernard M., Roseboom P.H., Klein D.C., Rodriguez I.R. (1996). The human serotonin N-acetyltransferase (EC 2.3.1.87) gene (AANAT): Structure, chromosomal localization, and tissue expression. Genomics. 15, 76-84.
Ding-ping B., Cheng-jiang Y. and Yu-lin C. (2012). Association between AA-NAT gene polymorphism and reproductive performance in sheep Electron. J. Biotechnol. 15, 7-17.
Dupre S.M., Burt D.W., Talbot R., Downing A., Mouzaki D., Waddington D., Malpaux B., Davis J.R., Lincoln G.A. and Loudon A.S. (2008). Identification of melatonin-regulated genes in the ovine pituitary pars tuberalis, a target site for seasonal hormone control. Endocrinology. 149, 5527-5539.
Fathy H.A., Gouda E.M., Gafer J.A., Galal M.K. and Nowier A.M. (2018). Genetic polymorphism in melatonin receptor 1A and arylalkylamine N-acetyltransferase and its impact on seasonal reproduction in Egyptian sheep breeds. Arch. Anim. Breed. 61, 505-516.
Foulkes N.S., Whitmore D. and Sassone-Corsi P. (1997). Rhythmic transcription: The molecular basis of circadian melatonin synthesis. J. Cell. Biol. 89, 487-494.
Gates P.J., Henningsson T., Tengroth G. and Forsberg M. (1998). Effects of melatonin, progestagens, and the ram on out-of- season reproduction in Swedish Landrace Finewool sheep. Acta Vet. 39, 499-510.
Giantsis I.A., Laliotis G.P., Stoupa O. and Avdi M. (2016). Polymorphism of the melatonin receptor 1A (MNTR1A) gene and association with seasonality of reproductive activity in a local Greek sheep breed. J. Biol. Res. 9, 23-29.
Gündoan M., Acar D. and Yeni D. (2003). Reproductive seasonality in sheep. Acta Agric. Scandinavica B. 53, 175-179.
Hafez E.S.E. (2013). Reproduction in Farm Animals. Lea and Febiger, Philadelphia, Pennsylvania.
Klein D.C. (2006). Evolution of the vertebrate pineal gland: The AANAT hypothesis. Chronobiol. Int. 5, 20-23.
Lewis R.M., Notter D.R., Hogue D.E and Magee B.H. (1996). Ewe fertility in the STAR accelerated lambing system. J. Anim. Sci. 74, 1511-1522.
Notter D.R., Cockett N.E. and Hadfield T.S. (2003). Evaluation of melatonin receptor 1A as a candidate gene influencing repro-duction in an autumn lambing sheep flock. J. Anim. Sci. 81, 912-917.
Oner Y., Orman A., Üstüner H. and Yilmaz A. (2014). Investigation of polymorphisms on ABCG2, AA-NAT and FABP3 genes in the Kıvırcık sheep reared in three different provinces of Turkey. Kafkas Univ. Vet. Fak. Derg. 20, 649-654.
Pereira D.S., Pedrazzoli M., Koike Bdel V., Louzada F.M., Benedito-Silva A.A., Lopez R.A. and Tufik S. (2007). The G619A Aa-nat gene polymorphism does not contribute to sleep time variation in the Brazilian population. Behav. Genet. 37, 637-648.
Rahawy M.A. and AL-Timimi I.H. (2016). Analysis of polymorphism of melatonin receptor type 1A (MTNR1A) gene, in Iraqi Local sheep using PCR-RFLP technique. Iraqi J. Biotechnol. 15, 85-95.
SAS Institute. (2002). SAS®/STAT Software, Release 9.1. SAS Institute, Inc., Cary, NC. USA.
Sharma R., Ahlawat S. and Tantia M.S. (2015). Novel polymorphism of AA-NAT gene in Indian goat breeds differing in reproductive traits. Iranian J. Vet. Res. 16, 377-380.
Soria V., Martínez-Amorós E., Escaramís G., Valero J., Crespo J.M., Gutiérrez-Zotes A., Bayés M., Martorell L., Vilella E., Estivill X., Menchón J.M., Gratacòs M. and Urretavizcaya M. (2010). Resequencing and association analysis of arylalkylamine N-acetyltransferase (AANAT) gene and its contribution to major depression susceptibility. J. Pineal Res. 49, 35-44.
Wang G.Y., Lee C.G.L. and Lee E.J.D. (2004). Genetic variability of arylalkylamine-N-acetyl-transferase (AA-NAT) gene and human sleep/wake pattern. Chronobiol. Int. 21, 229-237.
Yeh F.C., Yang R.C., Boyle T.B., Ye Z.H. and Mao J.X. (1997). POPGENE, the User-Friendly Shareware for Population Genetic Analysis. Molecular Biology and Biotechnology Centre, University of Alberta, Alberta, Canada.
Ying G.W., Lee C.G.L. and Lee E.J.D. (2004). A naturally occurring-263G/C variant of the human AA-NAT gene and overnight melatonin production. Mol. Genet. Metab. 81, 65-69.
Younis L.S. and Hatif S.A. (2017). AA-NAT melatonin gene polymorphism in ewes lambing out of season. Online J. Vet. Res. 21, 118-125.
