Effect of Thymus vulgaris and Satureja khuzestanica Ethanolic Extracts on Broiler Chickens’ Performance and Immune Response
الموضوعات :
ح. سوری
1
(Department of Animal Science, University of Ilam, Ilam, Iran)
ع. خطیبجو
2
(Department of Animal Science, University of Ilam, Ilam, Iran)
ک. طاهرپور
3
(Department of Animal Science, University of Ilam, Ilam, Iran)
ا. حسن آبادی
4
(Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran)
ف. فتاح نیا
5
(Department of Animal Science, University of Ilam, Ilam, Iran)
م. عسگری
6
(Department of Animal Science, University of Ilam, Ilam, Iran)
الکلمات المفتاحية: Performance, Broiler, immunity, <i>Saturejakhuzestanica</i> extract, <i>Thymus vulgaris</i> extract,
ملخص المقالة :
An experiment was conducted to investigate the effects of thyme (Thymus vulgaris) and satureja (Satureja khuzestanica) ethanolic extracts on the performance, blood metabolites and immune response of broiler chickens. 300 day-old Ross chicks were assigned to six dietary treatments in a randomized 2 × 3 factorial block design. Each treatment was given to five replicates of ten birds. Variables were T. vulgaris extract (0% or 1%) and S. khuzestanica extract (0%, 1% or 2%) in drinking water. Body weight (BW), feed intake (FI) and feed conversion ratio (FCR) was recorded at the end of the experiment. Serum glucose, total protein (TP), triglycerides (TG), low-density lipoprotein cholesterol (LDL-ch) and high-density lipoprotein cholesterol (HDL-ch) were measured after blood sampling at 42 days of age. Specific IgG and IgM against sheep red blood cells (SRBC) were quantified six days after the injection of SRBC into breast muscle on day 23 and day 30. The plant extracts did not affect BW, FI or FCR, or the relative weights of the cloacal bursa, spleen or thymus gland (P>0.05). S. khuzestanica extract increased TG, total cholesterol and HDL-ch (P<0.05). The plant extracts did not affect the humoral immune response against SRBC (P>0.05). However, 2% S. khuzestanica extract alone, or 1% T. vulgaris extract alone or in combination with 1% S. khuzestanica extract increased the heterophil percentage (H) and heterophil:lymphocyte ratio (H/L) (P>0.05), while it diminished the lymphocyte percentage (L) (P>0.05). Breast meat pH, redness (a*), yellowness (b*) and lightness (L*) were not affected by dietary treatments. However 2% and 1% S. khuzestanica extract respectively decreased thigh meat’s pH 24 h postmortem and its a* and b* values (P<0.05). 1% T. vulgaris extract, and 1% and 2% S. khuzestanica extract, increased pH in breast meat (P<0.05). It was concluded that under these research conditions, low levels of these extracts decreased H and H/L and may be beneficial to broiler chickens’ immunity.
Abdollahi M., Salehnia A., Mortazavi S., Ebrahimi M., Shafiee A., Fouladian F., Keshavarz K., Sorouri S., Khorasani R. and Kazemi A. (2003). Antioxidant, antidiabetic, antihyperlipidemic, reproduction stimulatory properties and safety of essential oil of Satureja Khuzestanica in rat in vivo: a oxicopharmacological study. Med. Sci. Monit. 9, 331-335.
Abdulkarimi R., Daneshyar M. and Aghazadeh A. (2011). Thyme (Thymus vulgaris) extract consumption darkens liver, lowers blood cholesterol, proportional liver and abdominal fat weights in broiler chickens. Italian J. Anim. Sci. 10, 101-105.
Al-Kassie G.A. (2009). Influence of two plant extracts derived from thyme and cinnamon on broiler performance. Pakistan Vet. J. 29, 169-173.
Al-Shami M., Salih M. and Abbas T. (2011). Effects of dietary inclusion of alfalfa (Medicago sativa) leaf meal and xylan enzyme on laying hens’ performance and egg quality. Res. Opin. Anim. Vet. Sci. 2, 14-18.
Ali M.N., Hassan M.S. and El-Ghany F.A. (2007). Effect of strain, type of natural antioxidant and sulphate ion on productive, physiological and hatching performance of native laying hens. Int. J. Poult. Sci. 6, 539-554.
Amanlou M., Dadkhah F., Salehnia A., Farsam H. and Dehpour A.R. (2005). An anti-inflammatory and anti-nociceptive effects of hydroalcoholic extract of Satureja khuzistanica Jamzad extract. J. Pharmal. Pharmacol. Sci. 8, 102-106.
Amresh G., Reddy G., Rao C.V. and Singh P. (2007). Evaluation of anti-inflammatory activity of Cissampelos pareira root in rats. J. Ethnopharmacol. 110, 526-531.
Barbut S. (1997a). Occurrence of pale soft exudative meat in mature turkey hens. Br. Poult. Sci. 38, 74-77.
Barbut S. (1997b). Problem of pale soft exudative meat in broiler chickens. Br. Poult. Sci. 38, 355-358.
Brewer M. (2011). Natural antioxidants: sources, compounds, mechanisms of action, and potential applications. Comp. Rev. Food Sci. Food Safety. 10, 221-247.
Case G.L., He L., Mo H. and Elson C.E. (1995). Induction of geranyl pyrophosphate pyrophosphatase activity by cholesterol-suppressive isoprenoids. Lipids. 30, 357-359.
Cheema M., Qureshi M. and Havenstein G. (2003). A comparison of the immune response of a 2001 commercial broiler with a 1957 randombred broiler strain when fed representative 1957 and 2001 broiler diets. Poult. Sci. 82, 1519-1529.
Chen H., Li D., Chang B.Y., Gong L., Dai J. and Yi G. (2003). Effects of Chinese herbal polysaccharides on the immunity and growth performance of young broilers. Poult. sci. 82, 364-370.
Choe J., Choi Y., Lee S., Shin H., Ryu Y., Hong K. and Kim B. (2008). The relation between glycogen, lactate content and muscle fiber type composition, and their influence on postmortem glycolytic rate and pork quality. Meat Sci. 80, 355-362.
CIE. (1978). International Commission on Illumination, Recommendations on Uniform Colour Spaces, Colour Difference Equations, Psychometric Colour Terms. CIE Publication. Bureau Central de la CIE, Paris, France.
Cook N. and Samman S. (1996). Flavonoids-chemistry, metabolism, cardioprotective effects, and dietary sources. J. Nutr. Biochem. 7, 66-76.
Dahal I. and Farran M. (2011). Effect of dried medicinal crops on the performance and carcase flavour of broilers. Int. J. Poult. Sci. 10, 152-156.
Delhanty J. and Solomon J. (1966). The nature of antibodies to goat erythrocytes in the developing chicken. Immunology. 11, 102-103.
Demir E., Kilinc K., Yildirim Y., Dincer F. and Eseceli H. (2008). Comparative effects of mint, sage, thyme and flavomycin in wheat-based broiler diets. Arch. Zootech. 11, 54-62.
Dorman H. and Deans S. (2000). Antimicrobial agents from plants: antibacterial activity of plant volatile oils. J. Appl. Microbiol. 88, 308-316.
El-Ghousein S.S. and Al-Beitawi N.A. (2009). The effect of feeding of crushed thyme (Thymus valgaris) on growth, blood constituents, gastrointestinal tract and carcass characteristics of broiler chickens. J. Poult. Sci. 46, 100-104.
Ertas O.N., Guler T., Çiftçi M., Dalkilic B. and Simsek U.G. (2005). The effect of an essential oil mix derived from oregano, clove and anise on broiler performance. Int. J. Poult. Sci. 4, 879-884.
Fritz Z., Schleicher A. and Kinal S. (1993). Effect of substituting milfoil, St. Johnswort and lovage for antibiotics on chicken performance and meat quality. Anim. Feed Sci. Technol. 2, 189-195.
Froning G. and Uijttenboogaart T. (1988). Effect of post-mortem electrical stimulation on color, texture, pH and cooking losses of hot and cold deboned chicken broiler breast meat. Poult. Sci. 67, 1536-1544.
Ghasemi R., Zarei M. and Torki M. (2010). Adding medicinal herbs including garlic (Allium sativum) and thyme (Thymusvulgaris) to diet of laying hens and evaluating productive performance and egg quality characteristics. Am. J. Anim. Vet. Sci. 5, 150-151.
Grimble R.F. (1994). Impact of nutrients on cytokine biology in infection. Trans. Royal Soci. Trop. Med. Hyg. 88, 615-619.
Hajhashemi V., Sajjadi S.E. and Zomorodkia M. (2011). Antinociceptive and anti-inflammatory activities of Bunium persicum essential oil, hydroalcoholic and polyphenolic extracts in animal models. Pharmacol. Biol. 49, 146-151.
Hoehler D., Lemme A., Ravindran V., Bryden W. and Rostagno H. (2005) Feed formulation in broiler chickens based on standardized ileal amino acid digestibility. Pp. 78-91 in Proc. 3rd Mid-Atlantic Nutr. Conf. Timonium, Maryland.
Jang I., Ko Y., Kang S. and Lee C. (2007). Effect of a commercial essential oil on growth performance, digestive enzyme activity and intestinal microflora population in broiler chickens. Anim. Feed Sci. Technol. 134, 304-315.
Jeacocke R.E. (1977). Continuous measurement of the pH of beef muscle in intact beef carcasses. J. Food Technol. 12, 375-386.
Kannan G., Heath J., Wabeck C. and Mench J. (1997). Shackling of broilers: effects on stress responses and breast meat quality. Br. Poult. Sci. 38, 323-332.
Khan R., Naz S., Nikousefat Z., Tufarelli V. and Laudadio V. (2012). Thymus vulgaris: alternative to antibiotics in poultry feed. World's Poult. Sci. J. 68, 401-408.
Klasing K.C., Laurin D.E., Peng R.K. and Fry D.M. (1987). Immunologically mediated growth depression in chicks: influence of feed intake, corticosterone and interleukin-1. J. Nutr. 117, 1629-1637.
Kohlert C., Van Rensen I., März R., Schindler G., Graefe E. and Veit M. (2000). Bioavailability and pharmacokinetics of natural volatile terpenes in animals and humans. Planta Med. 66, 495-505.
Lawrie R.A. (1998). The conversion of muscle to meat. Pp. 96-118 in Lawrie’s Meat Science.R.A. Lawrie, Ed. Woodhead Publishing Ltd., Cambridge, England.
Lee K.W., Everts H., Kappert H., Yeom K.H. and Beynen A. (2003a). Dietary carvacrol lowers body weight gain but improves feed conversion in female broiler chickens. J. Appl. Poult. Res. 12, 394-399.
Lee K.W., Everts H., Kappert H., Frehner M., Losa R. and Beynen A. (2003b). Effects of dietary essential oil components on growth performance, digestive enzymes and lipid metabolism in female broiler chickens. Br. Poult. Sci. 44, 450-457.
Lee S.J., Umano K., Shibamoto T. and Lee K.G. (2005). Identification of volatile components in basil (Ocimum basilicum) and thyme leaves (Thymus vulgaris) and their antioxidant properties. Food Chem. 91, 131-137.
Manach C., Regerat F., Texier O., Agullo G., Demigne C. and Remesy C. (1996). Bioavailability, metabolism and physiological impact of 4-oxo-flavonoids. Nutr. Res. 16, 517-544.
Mansoub N.H. (2011). Assessment on effect of thyme on egg quality and blood parameters of laying hens. Ann. Biol. Res. 2, 417-422.
Mikaili P., Sarahroodi S., Hemmati A., Koochak M. and Akbari Z. (2010). A histological study on the effects of aqueous extract of Althea officinalis on epithelial and submucosal mucocilliary system of rat trachea following inhalation of cigarette smoke. Iranian J. Pharmacol. Res. 3, 56-57.
Moghaddam F.M., Farimani M.M., Salahvarzi S. and Amin G. (2007). Chemical constituents of dichloromethane extract of cultivated Satureja khuzistanica. Evid. Based Complement. Alter. Med. 4, 95-98.
Ocak N., Erener G., Burak Ak F., Sungu M., Altop A. and Ozmen A. (2008). Performance of broilers fed diets supplemented with dry peppermint (Mentha piperita) or thyme (Thymus vulgaris) leaves as growth promoter source. Czech J. Anim. Sci. 53, 169-173.
Papinaho P., Fletcher D. and Buhr R. (1995). Effect of electrical stunning amperage and peri-mortem struggle on broiler breast rigor development and meat quality. Poult. Sci. 74, 1533-1539.
Peric L., Miloševic N., Dukic-Stojcic M. and Bjedov S. (2008) Effect of Phytogenic Products on Performance of Broiler Chicken. World Nutrition Forum, Mayrhofen, Austria, Nottingham University Press.
Prasad K. and Kalra J. (1993). Oxygen free radicals and hypercholesterolemic atherosclerosis: effect of vitamin E. Am. Heart J. 125, 958-973.
Qureshi M. and Havenstein G. (1994). A comparison of the immune performance of a 1991 commercial broiler with a 1957 randombred strain when fed “typical” 1957 and 1991 broiler diets. Poult. Sci. 73, 1805-1812.
Rahimi S., Zadeh Z.T., Torshizi M.K., Omidbaigi R. and Rokni H. (2011). Effect of the three herbal extracts on growth performance, immune system, blood factors and intestinal selected bacterial population in broiler chickens. J. Agric. Sci. Technolo. 13, 527-539.
Ross B. (2009). Ross 308 Broiler Production Manual.
Saadat M., Pournourmohammadi S., Donyavi M., Khorasani R., Amin G., Salehnia A.N. and Abdollahi M. (2004). Alteration of rat hepatic glycogen phosphorylase and phosphoenolpyruvate carboxykinase activities by Satureja khuzestanica Jamzad essential oil. J. Pharm. Pharmacol. Sci. 7, 310-314.
SAS Institute. (2001). SAS®/STAT Software, Release 8.1. SAS Institute, Inc., Cary, NC. USA.
Sengül T., Yurtseven S., Cetin M., Kocyigit A. and Sögüt B. (2008). Effect of thyme (T. vulgaris) extracts on fattening performance, some blood parameters, oxidative stress and DNA damage in Japanese quails. J. Anim. Feed Sci. 474, 45-46.
Stef L., Dumitrescu G., Drinceanu D., Stef D., Mot D., Julean C., Tatileanu R. and Corcionivoschi N. (2009). The effect of medicinal plants and plant extracted oils on broiler duodenum morphology and immunological profile. Romanian Biotech. Lett. 14, 4606-4614.
Swatland H. (1993). Paleness, softness and exudation in pork-review. Pp. 273-286 in Pork Quality: Genetic and Metabolic Factors. E. Poulanne and D.I. Demeyer,Eds. CAB International, Wallingford, UK.
Tampieri M.P., Galuppi R., Macchioni F., Carelle M.S., Falcioni L., Cioni P.L. and Morelli I. (2005). The inhibition of Candida albicans by selected essential oils and their major components. Mycopathologia. 159, 339-345.
Toghyani M., Tohidi M., Gheisari A.A. and Tabeidian S.A. (2010). Performance, immunity, serum biochemical and hematological parameters in broiler chicks fed dietary thyme as alternative for an antibiotic growth promoter. African J. Biotechnol. 9, 6819-6825.
Van Miert A. (1995). Pro-inflammatory cytokines in a ruminant model: pathophysiological, pharmacological and therapeutic aspects. Vet. Quarter. 17, 41-50.
Vosmerova P., Chloupek J., Bedanova I., Chloupek P., Kruzikova K., Blahova J. and Vecerek V. (2010). Changes in selected biochemical indices related to transport of broilers to slaughterhouse under different ambient temperatures. Poult. Sci. 89, 2719-2725.
Vosough-Ghanbari S., Rahimi R., Kharabaf S., Zeinali S., Mohammadirad A., Amini S., Yasa N., Salehnia A., Toliat T. and Nikfar S. (2010). Effects of Satureja khuzestanica on serum glucose, lipids and markers of oxidative stress in patients with type 2 diabetes mellitus: a double-blind randomized controlled trial. Evid. Based Complement. Alter. Med. 7, 465-470.
Zamani Moghaddam A., Ghanadi A., Gafarian A. and Shojadoost B. (2007) The effect of satureja hortensis on performance of broiler chickens and NDHI titers.Pp. 87-89 in Proc. 16th European Symp. Poult. Nutr., Strasburg, France.
