Effect of the Addition of Insulin Like Growth Factor-I (IGF-I) on Cryopreservation of Hariana Bull Semen
الموضوعات :G.V. Singh 1 , A. Kumar 2 , V. Sachan 3 , J.K. Agrawal 4 , A. Saxena 5
1 - College of Veterinary Science and Animal Husbandry, U.P. Pt. Deen Dayal Upadhyay Veterinary and Animal Science University, Mathura, India
2 - Animal Husbandry, U.P. Pt. Deen Dayal Upadhyay Veterinary and Animal Science University, Mathura, India
3 - Animal Husbandry, U.P. Pt. Deen Dayal Upadhyay Veterinary and Animal Science University, Mathura, India
4 - College of Veterinary Science and Animal Husbandry, U.P. Pt. Deen Dayal Upadhyay Veterinary and Animal Science University, Mathura, India
5 - Animal Husbandry, U.P. Pt. Deen Dayal Upadhyay Veterinary and Animal Science University, Mathura, India
الکلمات المفتاحية: cryopreservation, IGF-1, Hariana bull, post-thaw, pre-freeze,
ملخص المقالة :
This experiment was designed to study the effect of insulin like growth factor-I (IGF-I) on cryopreservation of Hariana bull semen. For this purpose, semen ejaculates were collected from four Hariana bulls using artificial vagina at biweekly interval. The semen samples which possess more than 70% initial progressive motility and above 500 million/mL spermatozoa concentration was subsequently subjected to processing for experiment. Semen samples were extended in egg yolk tris glycerol (EYTG) extender and split into four groups. IGF-I was added at 50 ng/80 × 106 spermatozoa, 100 ng/80 × 106 spermatozoa and 150 ng/80 × 106 spermatozoa in the group II, III, IV respectively and group I as control (without IGF-I). Semen samples were evaluated at pre-freeze and post-thaw stage for percent individual progressive motility, percent live spermatozoa, percent hypo-osmotic swelling (HOS) positive spermatozoa, percent spermatozoa with intact acrosome. A significant (p < 0.05) higher individual progressive motility, viability, HOS response and acrosomal integrity was observed at pre-freeze and post-thaw stage by using 150 ng/ml IGF-I. Concentration of 150 ng/mL IGF-I was found to be more beneficial in cryopreservation of Hariana bull spermatozoa as evidenced by post-thaw seminal parameter. It was concluded that IGF-I can be added to extender for improving cryosurvial of Hariana bull spermatozoa.
Baumber J., Ball B.A. and Linfor J.J. (2005). Assessment of the cryopreservation of equine spermatozoa in the presence of enzyme scavengers and antioxidants. American J. Vet. Res. 66, 772-779.
Bloom E. (1950). A one minute live-dead sperm stain by means of eosin-nigrosin. Fertil. Steril. 1, 176-177.
El-Sisy G.A., El-Nattat W.S. and El-Sheshtawy R.I. (2007). Buffalo semen quality, antioxidants and peroxidation during chilling and cryopreservation. J. Dairy Sci. 39, 157-165.
Hancock J.L. (1951). A staining technique for the study of temperature shock in semen. Nature. 169, 323-326.
Henricks D.M., Kouba A.J., Lackey B., Boone W.R. and Gray S.L. (1998). Insulin-like growth factor-1 and alpha-2 macroglobulin in seminal plasma correlation with semen quality. Hum Reprod. 11, 746-750.
Jeyendran R.S., VanderVen H.H., Perez-Pelaez M., Craboand B.G. and Zaneveld L.J.D. (1984). The concept of fructose utilization by bull sperm and its relation to fertility. J. Dairy Sci. 39, 157-163.
Kumar P., Suman., Pawaria S., Dalal J., Bhardwaj S., Patila S., Jerome A. and Sharma R.K. (2019). Serum and seminal plasma IGF-I associations with semen variables and effect of IGF-I supplementation on semen freezing capacity in buffalo bulls. Anim. Reprod. Sci. 204, 101-110.
Lechniak D., Kedzierski A. and Stanislawski D. (2002). The use of HOS test to evaluate membrane functionality of boar sperm capacitated in vitro.Reprod. Domest. Anim. 37, 378-379.
Lejeune H., Chuzel F., Thomas T., Avallet O., Habert R., Durand P. and Saez J. (1996). Paracrine -regulation of leydig cells. Anim. Endocrinol. Paris. 57, 55-63.
Recio-Pinto E., Rechler M.M. and Ishii D.N. (1996). Effects of insulin, insulin-like growth factor-II, and nerve growth factor on neurite formation and survival in cultured sympathetic and sensory neurons. J. Neurosci. 6, 1211-1219.
Sang-Min S., Suhee K., Jin-Gi H. and Yong-Jun K. (2014). IGF-I improves mitochondrial membrane potential during hypothermic storage of canine spermatozoa. J. Vet. Med. Sci. 76, 1065-1072.
Sauerweina H., Breier B.H., Gallaher B.W., Gotz C., Küfner G., Montag T., Vickers M. and Schallenberger E. (2000). Growth hormone treatment of breeding bulls used for artificial insemination improves fertilization rates. Domest. Anim. Endocrinol. 18, 145-158.
Schlingmann K., Michaut M.A., McElwee J.L., Wolff C.A., Travis A.J. and Turner R.M. (2007). Calmodulin and CaMKII in the sperm principal piece: Evidence for a motility-related calcium/calmodulin pathway. J. Androl. 28, 706-716.
Selvaraju S., Binsila B., Krishnan K., Archana S.S. and Ravindra J.P. (2016). IGF-I stablizes sperm membrane proteins to reduce cryoinjury and maintain post-thaw sperm motility in buffalo (Bubalus bubalis) spermatozoa. Cryobiology. 73(1), 55-62.
Selvaraju S., Reddy I.J., Nandi S., Rao S.B. and Ravindra J.P. (2009). Influence of IGF-I on buffalo (Bubalus bubalis) spermatozoa motility, membrane integrity, lipid peroxidation and fructose uptake in vitro. Anim. Reprod. Sci. 113, 60-70.
Sortino M.F. and Canonico P.L. (1996). Neuroprotective effects of insulin-like growth factor-I in immortalized hypothalamic cells. Endocrinology. 137, 1418-1425.
SPSS Inc. (2011). Statistical Package for Social Sciences Study. SPSS for Windows, Version 20. Chicago SPSS Inc., USA.
Stewart C.E. and Rotwein P. (1996). Growth, differentiation and survival: Multiple physiological functions for insulin-like growth factors. Physiol. Rev. 76, 1005-1026.
Vickers M.H., Casey P.J., Champion Z.J., Gravance C.G. and Breier B.H. (1999). Insulin-like growth factor-1 (IGF-1) and IGF-I receptor in human testis: an immune-histochemical study. Fertil. Steril. 49, 666-669.
Vishwanath R. and Shannon P. (1997). Do sperm cells age? A review of the physiological changes in sperm during storage at ambient temperature. Reprod. Fertil. Dev. 9, 321-332.
Wainer R., Merlet F., Bailly M., Lombroso R., Camus E. and Bisson J.P. (1996). Prognostic sperm factors in intra-uterine insemination with partner’s sperm. Contracept. Fertil. Sex. 24, 897-903
Watson P.F. (1975). Use of giemsa srain to detect changes in acrosomes of frozen ram spermatozoa. Vet. Record. 97, 12-15.
