Effect of in ovo Injection of Calcium Carbonate Nanoparticles on Bone Post Hatched Characteristics and Broiler Chicken Performance
الموضوعات :ج. سالاری 1 , ح.ر. همتی متین 2 , ک. غفاری 3 , ه. حاجتی 4
1 - Department of Animal Science, Faculty of Agriculture, Bu Ali Sina University, Hamedan, Iran
2 - Department of Animal Science, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran
3 - Department of Animal Veterinary, Ferdowsi University of Mashhad, Mashhad, Iran
4 - Department of Animal Science, Faculty of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
الکلمات المفتاحية: broiler performance, calcium carbonate nanoparticles, <i>in ovo</i>,
ملخص المقالة :
This experiment was conducted to evaluate the effect of in ovo injection (IOI) of calcium carbonate nano-particles (CCN) on post-hatch bone and blood parameters and performance of broiler chickens. Fertile eggs (n=400) were distributed into 4 groups of 100 eggs. On the first day of incubation, 2 groups were injected with 0.5 mL of either 100 or 200 ppm CCN/egg dissolved in normal saline and two groups were used as sham control (injected with 0.5 mL normal saline) or un-injected control. The hatched chicks from each group were randomly assigned to 4 replications of 15 chickens and reared under standard condition. The results demonstrated that body weight, spleen weight, and bone Ca concentration of hatched chickens was higher by IOI of 200 ppm/egg CCN (P<0.05). The highest bursa weight was obtained by IOI of 200 ppm/egg CCN (P<0.05). The IOI of CCN at levels of 100 or 200 ppm/egg was significantly increased plasma alkaline phosphatase activity and bone Cu concentration of hatched chickens (P<0.05). Moreover, IOI had no significant effects on performance at 1 to 21 days of age (P<0.05). These preliminary results suggest that growth and development of bone may accelerate through IOI of CCN at levels of 100 or 200 ppm/egg on 1-d of incubation.
Bhanja S.K., Mandal A.B. and Johari T.S. (2004). Standardization of injection site, needle length, embryonic age and concentration of amino acids for in ovo injection in broiler breeder eggs. Indian J. Poult. Sci. 39, 105-111.
Blake G.M. and Fogelman I. (2002). Methods and clinical issues in bone densitometry and quantitative ultrasonometry. Pp. 1573-1585 in Principles of Bone Biology. J.P. Bilezikian, L.G. Raisz and G.A. Rodan, Eds. Academic Press, San Diego, California.
Bourrin S., Ammann P., Bonjour J.P. and Rizzoli R. (2002). Recovery of proximal tibia bone mineral density and strength, but not cancellous bone architecture, after long-term bisphosphonate or selective estrogen receptor modulator therapy in aged rats. Bone. 30(1), 195-200.
Chen H.S., Chang J.H. and Wu J.S. (2008). Calcium bioavailability of nanonized pearl powder for adults. J. Food Sci. 73, 246-251.
Douroumis D. and Fahr A. (2006). Nano- and micro-particulate formulations of poorly water-soluble drugs by using a novel optimized technique. European J. Pharm. Biopharm. 63, 173-175.
Dziedzic-Gocławska A. (1995). Histology. PZWL, Poland.
Gralak M.A., Piastowska A.W., Leontowicz H., Leontowicz M., Antczak A., Kulasek G., Szara T. and Narojek T. (2004). Effect of dietary protein level and sources on bone mineralization and structure in rats. Biofactors. 22, 25-28.
Grodzik M., Sawosz F., Sawosz E., Hotowy A., Wierzbicki M., Kutwin M., Jaworski S. and Chwalibog A. (2013). Nano-nutrition of chicken embryos. The effect of in ovo administration of diamond nanoparticles and L-glutamine on molecular responses in chicken embryo pectoral muscles. Int. J. Mol. Sci. 14, 23033-23044.
Hamburger V. and Hamilton H.L. (1951). Series of normal stage in the development of the chick embryo. J. Morphol. 88, 49-92.
Kim Y.S., Kim J.S., Cho H.S., Rha D.S., Kim J.M., Park J.D., Choi B.S., Lim R., Chang H.K., Chung Y.H., Kwon I.H., Jeong J., Han B.S. and Yu I.J. (2008). Twenty-eight-day oral toxicity, genotoxicity, and gender-related tissue distribution of silver nanoparticles in Sprague-Dawley rats. Inhal. Toxicol. 20, 575-583.
Libby P. and Aikawa M. (2002). Vitamin C, collagen, and cracks in the plaque. Circu. 105, 1396-1398.
Merisko-Liversidge E.M. and Liversidge G.G. (2008). Drug nanoparticles: for- mulating poorly water-soluble compounds. Toxicol. Pathol. 36, 43-48.
NRC. (1994). Nutrient Requirements of Poultry, 9th Rev. Ed. National Academy Press, Washington, DC., USA.
Nir I. and Levanon M. (1993). Research note: effect of posthatch holding time on performance and on residual yolk and liver composition. Poult. Sci. 72, 1994-1997.
Nordin B.E.C., Gurr M.I., McIntosh G.H., Schaafsma G., Miller G.D., Groziak S.M., Sieber R. and Goulding A. (1997). Dietary calcium in health. Bull. Int. Dairy Fed. 322, 36-40.
Orban J.I., Adeola O. and Stroshine R. (1999). Microbial phytase in finisher diets of White Pekin ducks: effect on growth performance, plasma phosphorus concentration, and leg bone characteristics. Poult. Sci. 78, 366-377.
Park H.S., Ahn J. and Kwak H.S. (2008). Effect of nano-calcium-enriched milk on calcium metabolism in ovariectomized rats. J. Med. Food. 11, 454-459.
Pearson A.M. and Dutson T.R. (1992). Inedible meat by-products. Adv. Food Res. 8, 416-432.
Petracci M. and Cavani C. (2012). Muscle growth and poultry meat quality issues. Nutrients. 4, 1-12.
Salary J., Sahebi-Ala F., Kalantar M. and Hemati Matin H.R. (2014). In ovo injection of vitamin E on post-hatch immunological parameters and broiler chicken performance. Asian Pac. J. Trop. Biomed. 4(2), 733-736.
SAS Institute. (2008). SAS®/STAT Software, Release 9.2. SAS Institute, Inc., Cary, NC. USA.
Sawosz F., Pineda L., Hotowy A., Hyttel P., Sawosz E., Szmidt M., Niemiec T. and Chwalibog A. (2012). Nano-nutrition of chicken embryos. The effect of silver nanoparticles and glutamine on molecular responses, and the morphology of pectoral muscle. Baltic J. Comp. Clin. Syst. Biol. 2, 29-45.
Uni Z. and Ferket P.R. (2004). Methods for early nutrition and their potential. World’s Poult. Sci. J. 60, 101-111.
Uni Z., Ferket P.R., Tako E. and Kedar O. (2005). In ovo feeding improves energy status of late-term chicken embryos. Poult. Sci. 8, 764-770.
Zielinska M., Sawosz E., Grodzik M., Balcerak M., Wierzbicki M., Skomial J., Sawosz F. and Chwalibog A. (2012). Effect of taurine and gold nanoparticles on the morphological and molecular characteristics of muscle development during chicken embryogenesis. Arch. Anim. Nutr. 66, 1-13.
Zielinska M., Sawosz E., Grodzik M., Wierzbicki M., Gromadka M., Hotowy A., Sawosz F., Lozicki A. and Chwalibog A. (2011). Effect of heparan sulfate and gold nanoparticles on muscle development during embryogenesis. Int. J. Nanomed. 6, 3163-3172.
