In vivo Testis Transfection Efficiently Produced Transfected Sperm Cells in Ram but not Rooster
الموضوعات :
A. Ebnali
1
(Transgenesis Center of Excellence, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran)
A. Dehghani Poudeh
2
(Transgenesis Center of Excellence, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran)
A. Pirestani
3
(Transgenesis Center of Excellence, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran|Department of Animal Science, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran)
M. Forouzanfar
4
(Department of Biology, Islamic Azad University, Marvdasht Branch, Marvdasht, Iran)
S. Eghbalsaied
5
(Young Researchers and Elite Club, Isfahan (Khorasgan) branch, Islamic Azad University, Isfahan, Iran)
الکلمات المفتاحية: Sheep, chicken, sperm-mediated gene transfer, testis-mediated gene transfer, transgenesis,
ملخص المقالة :
We report a method for gene transfer via in vivo testis-mediated gene transfer (TMGT) in sheep. A non-viral vector, pDB2, which carried an enhanced green fluorescent protein (EGFP) transgene under control of a human cytomegalovirus (CMV) promoter, was mixed with the TransIT transfection reagent. The lipoplex mixture was injected intra-testicularly or into the cauda epididymis of 12 and 5 rams, respectively, as well as the intra-testis of 5 mature roosters. Each injected rooster was crossed with four virgin hens and their hatched chicks were assessed for the presence of the EGFP transgene. After 60 days, both rams and roosters were investigated for the transgene. Unlike roosters, polymerase chain reaction (PCR) analysis of sperm cells which were collected from the epididymis and seminiferous revealed that more than 50% of sperm samples from the intratesticular rete-injected group were PCR-positive for the EGFP transgene. Transgene uptake was also observed in seminiferous tubules and epididymis of the intratesticular rete and the cauda epididymis groups, respectively. In conclusion, the combined approach of TMGT and lipofection can lead to ovine sperm transfection. This approach has potential to be combined with the clustered regularly interspaced short palindromic repeat (CRISPR)-Cas technology and used for on-farm gene editing of sheep species.
Amini H.R., Pakdel A., Shahr-Babak H.M. and Eghbalsaied S. (2017). Developing a puncture-free in ovo chicken transfection strategy based on bypassing albumen nucleases. Theriogenology. 91, 90-97.
Barkhojasteh M., Abdolhay H.A., Gorjipoor E., Amini H., Salahi M.M. and Eghbalsaied S. (2020). Robust DNase activity of the ooplasm can act as a gametic transfection barrier in rainbow trout. Theriogenology. 142, 62-66.
Burrows W. and Quinn J. (1935). A method of obtaining spermatozoa from the domestic fowl. Poult. Sci. 14, 251-253.
Cardoso F. and Queiroz G. (1988). Duration of the cycle of the seminiferous epithelium and daily sperm production of Brazilian hairy rams. Anim. Reprod. Sci. 17, 77-84.
Chan A.W., Luetjens C.M., Dominko T., Ramalho-Santos J., Simerly C.R., Hewitson L. and Schatten G. (2000). Foreign DNA transmission by ICSI: Injection of spermatozoa bound with exogenous DNA results in embryonic GFP expression and live rhesus monkey births. Mol. Hum. Reprod. 6, 26-33.
Chaparian S., Abdulahnejad A., Rashidi F., Toghyani M., Gheisari A. and Eghbalsaied S. (2016). Is passive transmission of non-viral vectors through artificial insemination of sperm-DNA mixtures sufficient for chicken transgenesis? J. Reprod. Dev. 62, 265-270.
Deviche P., Hurley L.L. and Fokidis H.B. (2011). Hormones and Reproduction of Vertebrates. Elsevier, Amsterdam, Netherlands.
Eghbalsaied S., Ghaedi K., Hosseini S., Tanhaie S., Forouzanfar M., Hajian M., Mozafari N. and Esfahani M.N. (2009). Selection of the most appropriate medium for assessing motility and DNA uptake of bovine spermatozoa. Yakhteh Med. J. 10, 266-271.
Eghbalsaied S., Ghaedi K., Laible G., Hosseini S.M., Forouzanfar M., Hajian M., Oback F., Nasr-Esfahani M.H. and Oback B. (2013). Exposure to DNA is insufficient for in vitro transgenesis of live bovine sperm and embryos. Reproduction. 145, 97-108.
Eghbalsaied S. and Keus W. (2020). Development of a transposon-based technology for transfection of day 0 chicken embryos. Gene. 730, 144318-144326.
Esponda P. and Esponda P. (2005). Transfection of gametes: A method to generate transgenic animals. Int. J. Morphol. 23, 281-284.
González-Morán M.G., Guerra-Araiza C., Campos M.G. and Camacho-Arroyo I. (2008). Histological and sex steroid hormone receptor changes in testes of immature, mature, and aged chickens. Domest. Anim. Endocrinol. 35, 371-379.
Hasebe M., Soh T., Hattori M. and Fujihara N. (1998). An attempt to produce transgenic chicken mediating sperm cells as vectors. J. Appl. Anim. Res. 14, 143-150.
Hill J. and Dobrinski I. (2005). Male germ cell transplantation in livestock. Reprod. Fertil. Dev. 18, 13-18.
Honaramooz A., Behboodi E., Blash S., Megee S.O. and Dobrinski I. (2003). Germ cell transplantation in goats. Mol. Reprod. Dev. 64, 422-428.
Honaramooz A., Megee S.O. and Dobrinski I. (2002). Germ cell transplantation in pigs. Biol. Reprod. 66, 21-28.
Kim J.H., Jung Ha H.S., Lee H.T. and Chung K.S. (1997). Development of a positive method for male stem cell mediated gene transfer in mouse and pig. Mol. Reprod. Dev. 46, 515-526.
Laible G., Wei J. and Wagner S. (2015). Improving livestock for agriculture–technological progress from random transgenesis to precision genome editing heralds a new era. Biotechnol. J. 10, 109-120.
Lavitrano M., Bacci M.L., Forni M., Lazzereschi D., Di Stefano C., Fioretti D., Giancotti P., Marfé G., Pucci L. and Renzi L. (2002). Efficient production by sperm-mediated gene transfer of human decay accelerating factor (hDAF) transgenic pigs for xenotransplantation. Proc. Natl Acad. Sci. 99, 14230-14235.
Lavitrano M., Camaioni A., Fazio V.M., Dolci S., Farace M.G. and Spadafora C. (1989). Sperm cells as vectors for introducing foreign DNA into eggs: Genetic transformation of mice. Cell. 57, 717-723.
Lee Y.M., Jung J.G., Kim J.N., Park T.S., Kim T.M., Shin S.S., Kang D.K., Lim J.M. and Han J.Y. (2006). A testis-mediated germline chimera production based on transfer of chicken testicular cells directly into heterologous testes. Biol. Reprod. 75, 380-386.
McFarlane G.R., Salvesen H.A., Sternberg A. and Lillico S.G. (2019). On-farm livestock genome editing using cutting edge reproductive technologies. Front. Sustain. Food Syst. 3, 106-115.
McGrew M.J., Sherman A., Ellard F.M., Lillico S.G., Gilhooley H.J., Kingsman A.J., Mitrophanous K.A. and Sang H. (2004). Efficient production of germline transgenic chickens using lentiviral vectors. EMBO Rep. 5, 728-733.
Mostachio G.Q., De Oliveira L.D., Carciofi A.C. and Vicente W.R. (2008). The effects of anesthesia with a combination of intramuscular xylazine–diazepam–ketamine on heart rate, respiratory rate and cloacal temperature in roosters. Vet. Anaesth. Analg. 35, 232-236.
Nolan A., Livingston A. and Waterman A. (1986). The effects of alpha2 adrenoceptor agonists on airway pressure in anaesthetized sheep. J. Vet. Pharmacol. Ther. 9, 157-163.
Raina A., Kumar S., Shrivastava R. and Mitra A. (2015). Testis mediated gene transfer: In vitro transfection in goat testis by electroporation. Gene. 554, 96-100.
Rodriguez-Sosa J.R., Dobson H. and Hahnel A. (2006). Isolation and transplantation of spermatogonia in sheep. Theriogenology. 66, 2091-2103.
Salhab S., Zarkawi M., Wardeh M., Al-Masri M. and Kassem R. (2001). Development of testicular dimensions and size, and their relationship to age, body weight and parental size in growing Awassi ram lambs. Small Rumin. Res. 40, 187-191.
Sato M., Ishikawa A. and Kimura M. (2002). Direct injection of foreign DNA into mouse testis as a possible in vivo gene transfer system via epididymal spermatozoa. Mol. Reprod. Dev. 61, 49-56.
Sato M., Iwase R., Kasai K. and Tada N. (1994). Direct injection of foreign DNA into mouse testis as a possible alternative of sperm mediated gene transfer. Anim. Biotechnol. 5, 19-31.
Schusser B., Collarini E.J., Yi H., Izquierdo S.M., Fesler J., Pedersen D., Klasing K.C., Kaspers B., Harriman W.D. and van de Lavoir M.C. (2013). Immunoglobulin knockout chickens via efficient homologous recombination in primordial germ cells. Proc. Natl. Acad. Sci. 110, 20170-20175.
Wang H. and Cao W.G. (2013). Construction of transgenic mice by testicular efferent duct injection. Tianjin Agric. Sci. 4, 10-12.
Whitelaw C.B.A., Sheets T.P., Lillico S.G. and Telugu B.P. (2016). Engineering large animal models of human disease. J. Pathol. 238, 247-256.
