Effects of Different Preparations of Gonadotropins (oFSH and pFSH) on Superovulatory Response and Embryo Yield in Indigenous Ewes in Bangladesh
الموضوعات :
P.K. Jha
1
(Department of Surgery and Obstetrics, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh|Nepal Agricultural Research Council (NARC), Khumaltar, Lalitpur, Kathmandu, Nepal)
M. Golam Shahi Alam
2
(Department of Surgery and Obstetrics, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh)
M.R.I. Talukder
3
(Department of Surgery and Obstetrics, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh)
F.Y. Bari
4
(Department of Surgery and Obstetrics, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh)
الکلمات المفتاحية: Sheep, corpus luteum, synchronization,
ملخص المقالة :
Two different gonadotropins on superovulatory response and embryo yield in indigenous ewes were studied. Donor ewes (n=33) were synchronized for oestrus using vaginal sponges containing 45 mg flurogestone acetate (FGA) for 12 days. For the superovulation, different types of gonadotropins were used in ewes randomly allocated into group I [n=16; ovine follicle-stimulating hormone (oFSH), 2.2 mg] and group II [n=17; porcine follicle-stimulating hormone (pFSH), 25 mg]. Gonadotropin treatments started on day 10 of FGA treatment for four consecutive days. The superovulatory response and embryo recovery was performed by modified semi-laparoscopic method on day 5/6 following mating. The stages (very early morula, morula, early blastocyst, blastocyst and hatching blastocyst) and grades (grade 1 to 5) of embryos were calculated. The oestrus response rate was 100%, and had significantly earlier onset of oestrus with pFSH than oFSH (23.41±0.50 h vs. 26.15±0.46 h, respectively). The corpus luteum/donor was significantly higher with pFSH than oFSH (14.18±1.63 vs. 10.25±0.89, respectively). The embryos recovered/donor differed insignificantly with pFSH than oFSH (11.82±1.48 vs. 8.81±0.86, respectively). The embryo recovery rate was higher using oFSH than pFSH (85.04±2.93% vs. 82.75±3.31%, respectively). The proportion of morula and grade 1 embryos was differed insignificantly with both oFSH and pFSH (86.52% and 97.16% vs. 84.58% and 90.55%, respectively). In conclusion, both the oFSH and pFSH were equally efficient, giving consistent ovulation rate and embryo yield.
Ağaoğlu A.R., Kaymaz M., Korkmaz Ağaoğlu Ö., Karakaş K., PirYağcı İ. and Taşdemir U. (2012). Effect of different gonadotropin preparation on ovulatory response and embryo yield in Karayaka Ewes. Kafkas Univ. Vet. Fak. Derg. 18, 861-864.
Akoz M., Bulbul B., Ataman, M.B. and Dere S. (2006). Induction of multiple birth in Akkaraman cross-bred sheep synchronized with short duration and different doses of progesterone treatment combined with PMSG outside the breeding season. Bull. Vet. Inst. Pulawy. 50, 97-100.
Baldassarre H., Wang B., Kafidi N., Lazaris A. and Karatzas C.N. (2002). Advances in the production and propagation of transgenic goats using laparoscopic ovum pick-up and in vitro embryo production technologies. Theriogenology. 57, 275-284.
Bari F., Khalid M., Haresign W., Murray A. and Merrell B. (2000). Effect of mating system, flushing procedure, progesterone dose and donor ewe age on the yield and quality of embryos within a MOET program in sheep. Theriogenology, 53, 727-742.
Bari F.Y., Khalid M., Haresign W., Murry A. and Merrell B. (2003). Factors affecting the survival of sheep embryos after transfer within a MOET program. Theriogenology. 59, 1265-1275.
Bari F., Khalid M., Wolf B., Haresign W., Murray A. and Merrell B. (2001). The repeatability of superovulatory response and embryo recovery in sheep. Theriogenology. 56, 147-155.
Berlinguer F., Leoni G., Bogliolo L., Pintus P.P., Rosati I., Ledda S. and Naitana S. (2004). FSH different regimes affect the developmental capacity and cryotolerance of embryos derived from oocytes collected by ovum pick-up in donor sheep. Theriogenology. 61, 1477-1486.
Bettencourt E.M., Bettencourt C.M., Chagas de Silva J., Ferreira P., Manito C.I., Matos C.M., Romão R.J. and Rocha A. (2008). Effect of season and gonadotrophin preparation on superovulatory response and embryo quality in Portuguese Black Merinos. Small Rumin. Res. 74, 134-139.
Blanco M.R., Simonetti L. and Rivera O.E. (2003). Embryo production and progesterone profiles in ewes superovulated with different hormonal treatments. Small Rumin. Res. 47, 183-191.
Bülbül B., Gök K., Kızıl S.H., Kırbaş M., Çamlıdağ A., Satılmış M., Köse M., Seğmenoğlu M.S., Karaşahin T., Akbulut N.K., Kara U., Teke B.E. and Karakozak E. (2009). Comparison of superovulatory response given to two FSH pharmaceuticals containing different LH levels in holstein cows. Pp. 108-109 in Proc. 5th Int. Reprod. Art. Insem. Congr., Elazığ, Turkey.
Cordeiro M.F., Lima-Verde J.B., Lopes-Junior E.S., Teixeira D.I.A., Farias L.N., Salles H.O., Simplicio A.A., Rondina D. and Freitas V.J.F. (2003). Embry o recovery rate in Santa Ines ewes subjected to successive superovulatory treatments with p-FSH. Small Rumin. Res. 49, 19-23.
Cueto M.I., Gibbons A.E., Pereyra-Bonnet F., Silvestre P. and González-Bulnes A. (2010). Effects of season and superovulatory treatment on embryo yields in fine-wool Merinos maintained under field conditions. Reprod. Domest. Anim. 46, 770-775.
D'Alessandro A., Martemucci G., Toteda F., Gambacorta M. and Manchisi A. (1996). Superovulation and embryo production in ewes using a commercial p-FSH. Small Rumin. Res. 19, 255-261.
De Silva J.C., da Costa L.L., Cidadao R. and Silva J.R. (2003). Plasma progesterone profiles, ovulation rate, donor embryo yield and recipient embryo survival in native Saloia sheep in the fall and spring breeding seasons. Theriogenology. 60, 521-532.
Fernie K., McKelvey W.A.C. and Dingwall W.S. (1994). Endogenous FSH concentration in the ewe is not correlated to superovulatory response. Theriogenology. 41, 8-9.
Forcada F., Lozano J.M., Abecia J.A. and Zúñiga O. (2000). Repeated superovulation of high prolificacy Rasa Aragonesa ewes before culling as an inexpensive way to obtain high quality embryos. Livest. Prod. Sci. 66, 263-269.
Freitas V.J.F. and Melo L.M. (2010). In vitro embryo production in small ruminants. R. Bras. Zootec. 39, 409-413.
Gonzalez-Bulnes A., Santiago-Moreno J., Cocero M.J. and Lopez-Sebastian A. (2000a). Effects of FSH commercial preparation and follicular status on follicular growth and superovulatory response in spanish merino ewes. Theriogenology. 54, 1055-1064.
Gonzalez-Bulnes A., Santiago Moreno J., Gomez Brunet A. and Lopez-Sebastian A. (2000b). Relationship between ultrasonographic assessment of the corpus luteum and plasma progesterone concentration during the oestrous cycle in monovular ewes. Reprod. Domest. Anim. 35, 65-68.
Gonzalez-Bulnes A., Garcia-Garcia R.M., Souza C.J.H., Santiago-Moreno J., Lopez-Sebastian A., Cocero M.J. and Baird D.T. (2002). Patterns of follicular growth in superovulated sheep and influence on endocrine and ovarian response. Reprod. Domest. Anim. 37, 357-361.
Larsson B., Gustafsson A., Nasholm A. and Bjurström L. (2007). A programme for oestrus synchronization and embryo transfer in sheep. Reprod. Domest. Anim. 26, 300-308.
Lopes Jr E.S., Maia E.L.M.M., Paula N.R.O., Teixeira D.I.A., Villarroel A.B.S., Rondina D. and Freitas V.J.F. (2006). Effect of age of donor on embryo production in Morada Nova (white variety) ewes participating in a conservation programme in Brazil. Trop. Anim. Health Prod. 38, 555-561.
Mayorga I., Mara L., Sanna D., Stelletta C., Morgante M., Casu S. and Dattena M. (2011). Good quality sheep embryos produced by superovulation treatment without the use of progesterone devices. Theriogenology. 75, 1661-1668.
Naqvi S.M.K., Joshi A., Gulyani R., Kumar D., Kolte A.P., Kumar S., Maurya V.P., Saha S., Mittal J.P. and Singh V.K. (2006). Production of prolific microsheep by embryo transfer into large non-prolific sheep. Vet. Rec. 159, 522-526.
Pampukidou T., Alifakiotis M., Avdi R. and Ivanova I. (2011). Superovulation and embryo transfer in goats by using PMSG or FSH. Agric. Sci. Technol. 3, 94-97.
Roy P.K., Zohara B.F., Azizunnesa A., Paul A.K., Bhuiyan M.M.U. and Bari F.Y. (2014). Oestrus synchronization with Ovuprost® and Prostenol® in the indigenous ewes of Bangladesh. J. Emb. Trans. 29, 149-155.
Sánchez F., Bernal H., del Bosque A.S., González A., Olivares E., Padilla G. and Ledezma R.A. (2013). Superovulation and embryo quality with porcine follicle stimulation hormone (pFSH) in Katahdin hair sheep during breeding season. African J. Agric. Res. 8, 2977-2982.
Schiewe M.C., Howard J.G., Stuart L.S., Goodrowe K.L. and Wildt D.E. (1985). Human menopausal gonadotropin (HMG) for superovulation of sheep. Theriogenology. 23, 227-237.
Simonetti L., Blanco M.R. and Gardon J.C. (2000). Oestrus synchronization in ewes treated with sponges impregnated with different doses of medroxyprogesterone acetate. Small Rumin. Res. 38, 243-247.
SPSS Inc. (2011). Statistical Package for Social Sciences Study. SPSS for Windows, Version 20. Chicago SPSS Inc., USA.
Swelum A.A.A., Al-Owaimer A.N. and Abouheif M.A. (2014). Effects of breed and number of embryos transferred on the efficacy of MOET in sheep. J. Anim. Vet. Adv. 13, 98-103.
Zohara B.F. (2016). Development of multiple ovulation and embryo transfer (MOET) technique in indigenous ewes in Bangladesh, Ph D. Thesis. Bangladesh Agricultural Univ., Mymensingh, Bangladesh.
Zohara B.F., Azizunnesa A., Islam M.F., Alam M.G.S. and Bari F.Y. (2014a). Comparison of estrus synchronization by PGF2α and progestagen sponge with PMSG in indigenous ewes in Bangladesh. J. Vet. 1, 27-37.
Zohara B.F., Islam M.F., Alam M.G.S. and Bari F.Y. (2014b). Effects of cloprostenol and flurogestone acetate sponge on embryo yields and quality in indigenous ewes in Bangladesh. Anim. Prod. Sci. 54, 1605-1608.
Zohara B.F., Islam M.F., Alam M.G.S. and Bari F.Y. (2017). Survival of embryos after transfer within multiple ovulation and embryo transfer (MOET) programme. Small Rumin. Res. 149, 11-15.
