Association between DGAT1 Genotype and Breeding Value of Milk Production Traits in Iranian Holstein Bulls
الموضوعات :م. حسین پور مشهدی 1 , م. نصیری 2 , ن. امام جمعه کاشان 3 , ر. واعظ ترشیزی 4
1 - Department of Animal Science, Mashhad Branch, Islamic Azad University, Mashhad, Iran
2 - Department of Animal Science, Ferdowsi University of Mashhad, Mashhad, Iran
3 - Department of Animal Science, Science and Research Branch, Islamic Azad University, Tehran, Iran
4 - Department of Animal Science, Tarbiat Modares University, Tehran, Iran
الکلمات المفتاحية: breeding value, DGAT1 gene, Holstein bulls, milk production traits,
ملخص المقالة :
The aim of this study was to test the genotypes effect of DGAT1 gene on breeding value in Iranian Holstein milk production traits. For this purpose, 103 semen samples of registered bulls were collected and then genotyped for partial region of DGAT1 gene. The studied traits were milk yield (MY), fat yield (FY), protein yield (PY), fat percentage (FP) and protein percentage (PP). First lactation records of 43044 dairy cattle from 2000 to 2007 were used. The PCR-RFLP method was used for genotyping DGAT1 gene. The Hardy Weinberg equilibrium for allele and genotype frequencies was analyzed with Chi-square test using PoP-Gen software. Genetic parameters of studied traits were estimated by REML method under animal model with DFREML software. The breeding value of individual was predicted by BLUP method. Different genotype effect of 97 of 103 proven bulls on breeding value of milk production traits were investigated by GLM procedure of SAS (9.1) software.The mean of breeding value of proven bulls with different genotype were compare with Duncan test. The observed genotype frequency results showed KK (0.592), KA (0.408) and AA (zero) and the allele frequency obtained was K (0.7961) and A (0.2039). The KK genotype was confirmed by sequencing method and the K allele, with long of 411 bp, was registered in gene bank of NCBI with accession number EU077528. Estimated heritability of studied traits were 0.35 (0.02), 0.33 (0.02), 0.31 (0.017), 0.28 (0.02) and 0.27 (0.016) for MY, FY, PY, FP and PP, respectively. The average breeding values of MY, FY, PY, FP and PP were estimated as: 180.2 (28.8) kg, 3.7 (1.26) kg, 2.3 (1.06) kg, -0.036 (0.014) % and -0.03 (0.01) %, respectively. The average breeding value of MY for KA and KK genotypes were 288.8 and 109.6 kg, respectively and the difference was significant (P<0.01). The average breeding values of FY in bulls for KK and KA genotypes were 5.6 kg and 0.91 kg, respectively (P>0.05). The average breeding values of PY for KA and KK genotypes were 0.025 and 5.5 kg, respectively (P<0.05). Differences between means of breeding values for FP and PP were significant (P<0.05), these values for genotype KK and KA were -0.009% and -0.067% for fat and -0.016% and -0.059% for protein, respectively.
Boichard D., Grohs C., Bourgeois F., Cerqueira F., Faugeras R., Neau A., Rupp R., Amigues Y., Boscher M.Y. and Levézil H. (2003). Detection of genes influencing economic traits in three French dairy cattle breeds. Genet. Sel. Evol. 35, 77-101.
Boom R., Sol C.J.A., Salimans M.M.M., Jansen C.L., Wertheimvandillen P.M.E. and Vandernoordaa J. (1990). Rapid and simple method for purification of nucleic-acids. J. Clin. Microbiol. 28(3), 495-503.
Citek J., Rehout V., Hradecka E., Vecerek L. and Panicke L. (2004). The breeding values of German Holstien sires and the DGAT1 polymorphism. Arch. Tierz. 50, 125-135.
Coppieters W., Riquet J., Arranz J.J., Berzi P., Cambisano N., Grisart B., Karim L., Marcq F., Moreau L., Nezer C., Simon P., Vanmanshoven P., Wagenaar D. and Georges M. (1998). A QTL with major effect on milk yield and composition maps to bovine chromosome 14. Mamm. Genome. 9(7), 540-544.
Dadpasand M., Miraei ashtiani S.R., Moradi Shahrebabak M., Vaez Torshizi R. (2008). Impact of conformation traits on functional longevity of Holstein cattle of Iran assessed by a Weibull proportional hazards model. Livest. Sci. 118(3), 204-211.
Farnir F., Grisart B., Coppieters W., Riquet J., Berzi P., Cambisano N., Karim L., Mni M., Moisio S., Simon P., Wagenaar D., Vilkki J. and Georges M. (2002). Simultaneous mining of linkage and linkage disequilibrium to fine map quantitative trait loci in outbred half-sib pedigrees revisiting the location of a quantitative trait locus with major effect on milk production on bovine chromosome 14. Genetics. 161(1), 275-287.
Grisart B., Coppieters W., Farnir F., Karim L., Ford C., Berzi P., Cambisano N., Mni N., Reid S., Simon P., Spelman R., Georges M. and Snell R. (2001). Positional candidate cloning of a QTL in dairy cattle: identification of a missense mutation in the bovine and composition. Genome. Res. 12, 222-231.
Grisart B., Farnir F., Karim L., Cambisano N., Kim J.J., Kvasz A., Mni N., Simon P., Frere J.M., Coppieters W. and Georges M. (2004). Genetic and functional confirmation of the causality of the DGAT1 K232A quantative trait nucleotide in affecting milk yield and composition. Proc.Nation.Acad. Sci. 101(8), 2398-2403.
Heyen D.W., Weller J.I., Ron M., Band M., Beever J.E., Feldmesser E., Da Y., Wiggans G.R., VanRaden P.M. and Lewin H.A. (1999). A genome scan for QTL influencing milk production and health traits in dairy cattle. Physiol. Genom. 1(3), 165-175.
Kaupe B., Brandt H., Prinzenberg E.M. and Erhardt G. (2007). Joint analysis of the influence of CYP11B1 and DGAT1 genetic variation on milk production, somatic cell score, conformation, reproduction and productive lifespan in German Holstein cattle. J. Anim. Sci. 85, 11-21.
Kühn C., Thaller G., Winter A., Bininda Emonds O., Kaupe B., Erhart G., Bennewitz J., Schwerin M. and Fries R. (2004). Evidence for multiple alleles at the DGAT1 locus better explains a quantitative trait locus with major effect on milk fat content in cattle. Genetics. 50,1873-1881.
Meyer K. (2000). DFREML. Program to estimate variance component for individual animal models by restriction maximum likelihood (REML). Ver 3.1. User notes. Anim. Genet. Breed. Unit Univ. New England, Armdle.
Sanger F., Nicklen S. and Coulson A.R. (1977). DNA sequencing with chain terminating inhibitors. Proc. Nat. Acad. Sci. 74, 5463-5467.
Spelman R.G., Ford C.A., McElhinney P., Gregory G.C. and Snell R.G. (2002). Characterization of the DGAT1 gene in the New Zealand dairy population. J. Dairy Sci. 85, 3514-3517.
Thaller G., Krämer W., Winter A., Kaupe B., Erhardt G. and Fries R. (2003). Effect of DGAT1 variants on milk production traits in German cattle breeds. J. Anim. Sci. 81, 1911-1918.
Winter A., Alzinger A. and Fries R. (2004). Assessment of the gene content of the chromosomal regions flanking bovine DGAT1. Genomics. 83, 172-180.
Winter A., Krämer W., Werner F.A.O., Kollers S., Kata S., Durstewitz G., Buitkamp J., Womack J.E., Thaller G. and Fries R. (2002). Association of lysine-232 / alanine polymorphism in a bovine gene encoding acyl-CoA: diacylglycerol acyltransferase (DGAT1) with variation at a quantitive trait locus for milk fat content. Proc. Nation. Acad. Sci. 99(14), 9300-9305.
