Association of Bovine Lactoferrin Gene with Mastitis in Frieswal Cattle
الموضوعات :
پ. شارما
1
(Animal Genetics and Breeding, College of Veterinary Science, Nanaji Deshmukh Veterinary Science University, Jabalpur, India)
اس.ان.اس. پارمار
2
(Animal Genetics and Breeding, College of Veterinary Science, Nanaji Deshmukh Veterinary Science University, Jabalpur, India)
ام.اس. تاکور
3
(Animal Genetics and Breeding, College of Veterinary Science, Nanaji Deshmukh Veterinary Science University, Jabalpur, India)
د.اس. نایوریال
4
(Department of Veterinary Medicine, College of Veterinary Science, Anand Agricultural University, Anand, India)
ر. رنجان
5
(Animal Genetics and Breeding, College of Veterinary Science, Nanaji Deshmukh Veterinary Science University, Jabalpur, India)
الکلمات المفتاحية: PCR-RFLP, subclinical mastitis, LTF gene,
ملخص المقالة :
ThirtyFrieswal lactating cows were screened for clinical and subclinical mastitis and subsequently classified into healthy, subclinically affected and clinically affected groups each group comprising of 10 cows. Polymorphism of cow lactoferrin (LTF) gene promoter was determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The results showed that LFT gene promoter was polymorphic among different groups which indicated that its polymorphism was correlated to mastitis infection. The allelic frequency of C and G allele was 0.42 and 0.58 and they were associated with clinical and subclinical mastitis. It was found that LTF gene polymorphism and mastitis are highly associated with each other.
Gonzalo C., Martinez J.R., Carriedo J.A. and Primitivo F.S. (2003). Fossomatic cell-counting on ewe milk: Comparison with direct microscopy and study of variation factors. J. Dairy Sci. 86, 138-145.
Guo-Hua Li,Zhang Y., Dong S.X. and Li N. (2007). Study on the polymorphism of bovine lactoferrin gene and its relationship with mastitis. J. Anim. Biotechnol. 15(1), 67-76.
IDF. (1984). International Dairy Federation. Recommended methods for somatic cell counting in milk. Doc. No. 168, Int. Dairy Fed., Brussels, Belgium.
Jemmali B., Kamoun M., Ksouri M., Rekik B. and Mhirsi S. (2011). PCR-RFLP analysis of genetic polymorphism of the lactoferrin gene in Tunisian imported Holsteins. Way J. Anim. Sci. 2012, 170-173.
John S.W., Weitzner G., Rozen R. and Scriver C.R. (1991). A rapid procedure for extracting genomic DNA from leukocytes. Nucleic Acids Res. 19(2), 408.
Lakshmi B.S., Gupta B.R., Prakash M.G., Sudhakar K. and Sharma S. (2010). Genetic analysis of the production performance of Frieswal cattle. Tamil. J. Vet. Anim. Sci. 6(5), 215-222.
Motwani K.T. (2011). Mastitis in dairy cattle in India. Available at:
Nash D.L., Rogers G.W., Cooper J.B. and Keown J.F. (2000). Heritability of clinical mastitis incidence and relationships with sire transmitting abilities for somatic cell score, uddertypetraits, productive life and protein yield. J. Dairy Sci. 83(10), 2350-2360.
Roger Mellenberger. (2000). California Mastitis Test (CMT). An Invaluable Tool for Managing Mastitis, MichiganStateUniversity.
Schalm O.W. and Norlander D.O. (1957). Experiments and observations leading to the development of California Mastitis Test. J. Am. Vet. Med. Assoc. 130, 199-204.
Seyfert H.M., Tuckoricz A., Interthal H., Koczan D. and Hobom G. (1994). Structure of the bovine lactoferrin encoding gene and its promoter. Gen. 143, 265-269.
Wojdak-Maksymiec K., Kmiec M. and Ziemak J. (2006). Association between bovine lactoferrin gene polymorphism and somatic cell count in milk. Vet. Med. 51(1), 14-20.
Xingping W., Shangzhong X., Xue G., Hongyan R. and Jinbao, Chen (2007). Genetic polymorphism of TLR4 gene and correlation with mastitis in cattle. J. Genet. Genom. 34(5), 406-412.
Zhao C., Gao-ming H.E., Yan-liang W. and Zhao-xia Z. (2008). Polymorphism analysis of the promoter of cow lactoferrin gene with PCR-RFLP and its correlation with subclinical mastitis. Acta. Agri. Slov. 92(2), 185-187.
