تاثیر قارچ اندوفیت Piriformospora indica بر بهبود شاخص های رشد و فعالیت آنزیم های آنتی اکسیدان در گیاه برنج(Oryza sativa L.) تحت تنش شوری
الموضوعات :
علی اصغر باقری کشتلی
1
(گروه زیست شناسی، واحد رودهن، دانشگاه آزاد اسلامی، رودهن، ایران)
فریبا خسروی نژاد
2
(گروه زیست شناسی، واحد رودهن، دانشگاه آزاد اسلامی، رودهن، ایران)
الکلمات المفتاحية: تنش شوری, Piriformospora indica, آنزیمهای آنتیاکسیدان, برنج ) Oriza sativa (,
ملخص المقالة :
تنش های محیطی از جمله شوری، مهمترین عوامل محدود کننده رشد و عملکرد گیاهان زراعی در مناطق خشک و نیمهخشک هستند. قارچ اندوفیت Piriformospora indica دارای خاصیت تحریک رشد گیاه و افزایش مقاومت آن بهتنشهای محیطی، از جمله شوری، خشکی و بیماریهای گیاهی میباشد. در این پژوهش توان قارچ اندوفیت P. indicaدر بهبود پارامترهای رشد و افزایش مقاومت به تنش شوری گیاه برنج (Oryza sativa L.) با تکیه بر افزایش فعالیتآنزیمهای آنتی اکسیدان مورد مطالعه قرار میگیرد . بدین منظور، آزمایش گلخانه ای در قالب طرح بلوکهای کاملتصادفی با 3 تکرار و با دو تیمار شامل 2 سطح قارچ تلقیح شده و تلقیح نشده )شاهد( و 4 سطح شوری 0 ، 100 ، 200 و300 میلی مولار نمک ) NaCl ( انجام گرفت. نتایج حاصل از این پژوهش نشان دادکه قارچ P. indica سبب افزایشپارامترهائی رشدی مانند بیومس )زیتوده( اندامهای هوایی و ریشه، محتوای نسبی آب، محتوای پرولین و فعالیتهایآنزیم های آنتی اکسیدانی چون گلوتاتیون ردوکتاز، آسکوربات پراکسیداز، کاتالاز و سوپر اکسید دیسموتاز و کاهش تولیدمالون دی آلدئید ) حاصل از پراکسیداسیون لیپیدهای غشائی ( گیاهان تلقیح شده نسبت به گیاهان شاهد در تمام سطوحشوری شد. بنابراین میتوان نتیجه گرفت که قارچ اندوفیت P.indica ب ه لحاظ بهبود سیستم دفاعی آنتیاکسیدانیخطرات ناشی از اثر تنش شوری بر رشد گیاه برنج را تعدیل مینماید.
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