Study of Diversity for Yield and Quality Traits in Alfalfa (Medicago sativa L.) and Determination of the Best Population for Cultivation in Dryland Farming in Iran
الموضوعات :Mohsen Farshadfar 1 , Mehdi Kakaei 2 , Yaser Salehabadi 3 , Zahra Baghaeifar 4 , Ali ashraf Jaffari 5
1 - Department of Agriculture, Payame Noor University, Tehran, Iran
2 - Department of Agriculture, payame Noor University, Tehran, Iran
3 - Department of toxicology and pharmacology, faculty of pharmacy, Mazandaran University of Medical Sciences, Sari, Iran
4 - Department of Biology, Payame Noor University, Tehran, Iran
5 - Professor of plant breeding. Reaserch Institute of Forestry and Rangland,
الکلمات المفتاحية: Biodiversity, alfalfa, Forage yield, Nutritional value,
ملخص المقالة :
Alfalfa (Medicago sativa L.) is one of the most important forage crops, the so-called queen of forage plants, duo to its good quality, high digestibility, and its adaptability to different climates. In this study, 51 alfalfa populations were provided from the Research Institute of the Forests and Rangelands Gene Bank, Tehran, Iran. Seeds were sown based on an augmented design using six control genotypes in the agriculture and natural resources research center, Kermanshah, Iran in 2017. In the flowering stage, plants were cut and forage yield and chemical, quantitative and morphological traits were measured in all of 51 entries. Data were analyzed for descriptive statistics, correlation, factor analysis and cluster analysis. The Result of analysis of variance revealed no significant differences among replications (for replicated genotypes) for all traits except shoot height, leaves/stems, calcium, potassium, and total ash. The results of means comparison showed a significant variation between genotypes for the most studied traits. The highest and lowest forage yield with average values of 10089 and 1824 kg/h was obtained in Sharab-Urmia (Es-053) and Torbati1 (Es-032), respectively. The high protein content with average values of 19.46% was obtained in Sirjan1 (Es-026). Forage yield was positively correlated with stem number (r=0.50**), leaf weight (r=0.95**) and shoot weight (r=0.92**). Cluster analysis Ward method classified all alfalfa based on the all traits into four clusters. Populations in cluster 4 had higher overall mean values for both yield and quality traits. According to the Principle component analysis (PCA), the four components, namely the quality, yield, plant height and Mg+Zn components account for 40, 20, 10 and 7% (In total 77%) of data variance, respectively. The 5 top genotypes as FAO 1 (KR-3003), Cody 2 (Es-058), Italy 2 (Es-75), Kazagi2 (KR-615) and Mashhad 2 (Es-067) were recommended for improved breeding synthetic variety.
Agricultural Statistics of the Crop Year, 2019-20. Ministry of Jihad-Agriculture. Volume One: Crop Products.
Amanda, M., Grev, M., Scott Wells, D.N., Catalano, K.L., Martinson, J.M.J. and Craig, C.S., 2020. Stem and leaf forage nutritive value and morphology of reduced lignin alfalfa, Agronomy Journal, 112(1): 406-417.
Annicchiarico, P., Bottazzi, P., Ruozzi, F., Russi, L., and Pecetti, L. 2020. Alfalfa cultivar adaptation to Italian geographic areas is affected crucially by the selection environment and encourages the breeding for specific adaptation. Euphytica, 216: 20-28.
Bouton, J.H. 2012. Breeding alfalfa for persistence. Crop Pasture Sci. 63, 95–106.
Davodi, M., Jafari, A. A., Assadian G. and Ariapour, A. 2011. Assessment of Relationships among yield and quality traits in alfalfa (Medicago sativa) under dryland farming system, Hamadan, Iran. Jour. Rangeland Science. 1(2): 247-254.
El-Hifny, M.Z., B.R. Bakheit, M.S. Hassan, and W.A. Abd El-Rady, 2019. Forage and Seed Yield Variation of alfalfa Cultivars in Response to Planting Date. SVU-International Journal of Agricultural Science. 1(1): 21-33.
Eziz, A., Z. Yan, D. Tian, W. Han, Z. Tang, and J. Fang. 2017. Drought effect on plant biomass allocation: A meta-analysis. Ecol. Evol. 7: 11002-1110.
Farshadfar, M., 2016. Diversity and Relationships of Yield and Quality Traits in Cocksfoot (Dactylis glomerata L.) Genotypes. Jour Rangeland Science 7(3): 210-219.
Guines, F., B. Julier, C. Ecalle, C. Huyghe, 2002. Genetic control of quality traits of alfalfa (Medicago sativa L.). Australian Journal of Agricultural Research, 53: 401–407.
Hakl, J., Mofidian, S.M.A., Fuksa, P., and Jaromir, S., 2019. Estimation of alfalfa yield stability for enabling effective cultivar selection under rainfed conditions. Grass and forage Sci, 74(4): 687-695.
Jafari, A.A., Lak, M., and Assadian, G.2012. Variability and Correlation between the Seed Yield and its Component in Alfalfa (Medicago sativa L.) Populations under Dry Land Farming System, Hamadan, Iran. Jour. Rangeland Science 2 (3): 567-576
Jafari, A., Nosrati Nigeh, M. and Haidari Sharifabadm, H. 2003a. Comparison of yield, morphological and quality traits in 18 ecotypes and varieties of alfalfa (Medicago sativa) grown under irrigated and non-irrigated conditions. Proceeding of the VIIth International Rangelands congress, pages 1403-1405. Durban, South Africa.
Jafari, A., V. Connolly, A. Frolich and E.J. Walsh. 2003b. A note on estimation of quality parameters in perennial ryegrass by near infrared reflectance spectroscopy, Irish journal of agricultural and food research 42: 293-299
Joann, F.S., Lamb, G., Jungm H.J. and Heathcliffe, R., 2012. Harvest Impacts on alfalfa Stem Neutral Detergent Fiber Concentration and Digestibility and Cell Wall Concentration and Composition, Crop Science, 52 (5): 2402- 2412.
Joann, F.S.L., Hans‐Joachim, G.J., and Deborah, A.S., 2014. Environmental Variability and/or Stability of Stem Fiber Content and Digestibility in alfalfa, Crop Sci, 54(6): 2854-2863.
Marita, J.M.; Ralph, J.; Hatfield, R.D.; Guo, D.; Chen, F.; Dixon, R.A. 2020. Structural and compositional modifications in lignin of transgenic alfalfa down-regulated in caffeic acid 3-O-methyl-transferase and caffeoyl coenzyme A 3-O-methyltransferase. Phytochemistry 2003, 62, 53–65.
Martiniello, P., Baviello, G. and lamascese, N., 1994. Phenotypic variability for morphological and yield parameters in woody forage accessions of saltbush (Atriplex halimusl) and tree- medic (Medicago avborea L.). Jour. Genetic and Breeding, 48: 175-181.
Milić, D., D. Karagić, S. Vasiljevic, A. Mikić, B. Miloševic and S. Katić. 2014. Breeding and improvement of quality traits in alfalfa (Medicago sativa ssp. sativa L.). Genetika. 46: (1) 11-18.
Milić, D., S. Katić, A. Miklić, D. Karagić, J. Gvozdanović–Varga, S. Petrovicć and J. Boć anski, 2011. Genetic control of agronomic traits in alfalfa (M. sativa ssp. sativa L.). Euphytica. 182: 25–33.
Moore, J. E. and D. J. Undersander. 2002. Relative forage quality, an alternative to relative feed value and quality index. Proceeding 13th Annual Florida Ruminant Nutrition Symposium, 10–11 January, Florida, USA.
Mousavi, F., Heidari Sharifabadb, H. and Allahdoo, M. 2010. Investigation of genetic diversity in alfalfa ecotypes collection using multivariate analysis based on morphological traits. Plant Ecophysiology. 2: 103-108 (In Persian).
Pecetti, L., Annicchiarico, P., Scotti, C., Paolini, M., Nanni, V., Palmonari, A., 2017. Effects of plant architecture and drought stress level on alfalfa forage quality. Grass and Forage Science, 72(4). 714-722.
Porqueddu, C., Franca, A., G. Lombardi, G., Molle, G., Peratoner, G., Hopkins, A. 2017. Grassland resources for extensive farming systems in marginal lands: major drivers and future scenarios Proceedings of the 19th Symposium of the European Grassland Federation Alghero, Italy.
Radovic, J., Sokolovic, D., and Markovic, J., 2009. Alfalfa-Most important perennial forage legume in animal husbandry. Biotechnol. Anim. Husb, 25: 465–475.
Riday, H. and E.C. Brummer. 2004. Relationships among biomass yield components within and between subspecies of alfalfa. Medicago Genetic Reports, 4: 1-7.
Russell, M., 2001. Alfalfa: After an 8000-year journey, the "Queen of Forages" stands poised to enjoy renewed popularity. Am. Sci, 89: 252–261.
Sayar, M.S., Han, Y., Yolcu, H. and Yucel, H., 2014. Yield and quality traits of some perennial forages as both sole crops and intercropping mixtures under irrigated conditions. Turkish Jour. Field Crops, 19(1): 59-65.
Small, E. and Jomphe, M., 1988. A synopsis of the genus Medicago (Leguminosae). Canadian Jour. Botany, 67: 3260-3294.
Veronesi, F., Brummer, E.C., and Huyghe, C. 2010. Alfalfa. p. 395-437. In Boller, B., Posselt, U.K., and Veronesi, F. (eds.) Handbook of plant breeding: Fodder crops and amenity grasses. Springer, New York, USA.
Vivianne, F., Burnett, J.R. Hirth, K.L. Butler, M.L. Mitchell, S.G.C. and Zhongnan, N., 2018. The sensitivity of Winter-Active alfalfa (Medicago sativa L.) to Different Grazing Regimes. Agronomy, 8: 124.