The Possible Protective Role of Vitamin E on the Induced Silver Nanoparticles Toxicity on Filiform and Circumvallate Tongue Papillae of Albino Rats Histological and Immunohistochemical Study
الموضوعات :
Nada Tarek Zaki
1
(Demonstrator of Oral Biology, Faculty of Dentistry, The British University in Egypt, Cairo, Egypt)
Mahmoud Mohamed Al Ankily
2
(Lecturer of Oral Biology, Faculty of Dentistry, The British University in Egypt, Cairo, Egypt)
Reham Magdy Amin
3
(Professor of Oral Biology, Faculty of Dentistry, Ain Shams University, and The British University in Egypt, Cairo, Egypt)
Ahmed Mahmoud Halawa
4
(Professor of Oral Biology, Faculty of Dentistry, Ain Shams University, Cairo, Egypt)
الکلمات المفتاحية: vitamin E, silver nanoparticles, Nanotoxicity, Filiform, Circumvallate,
ملخص المقالة :
Recently Silver nanoparticles (AgNPs) are widely used as in manufacturing of filters to purify drinking water, as a part of surgical prosthesis, splints, dental alloys and most importantly in the medical field as a bactericidal, fungicidal and as a therapeutic agent. Although these widely usage of AgNPs, can induce toxicity. to evaluate the protective role of vitamin Eon the induced silver nanoparticles toxicity on filiform and circumvallate tongue papillae of albino rats. twenty-eight male albino rats weighing 200-250 grams were divided into; the control and the experimental groups, which is subdivided into, subgroup I receiving AgNPs and subgroup II receiving AgNPs and vitamin E. the experimental period were 28 days, then rats’ tongue was dissected to be stained by H&E and examined immunohistochemically for BCL-2. Histological examination of the epithelium covering both filiform and circumvallate papillae of subgroup I showed few epithelial cells vacuolations and signs of degeneration. Meanwhile the taste buds’ cells of the circumvallate papillae also showed signs of degeneration together with amalgamated, ill-defined serous acini of Von Ebner Salivary Gland (VESG). Subgroup II showed that the histological features of both tongue papillae were nearly comparable to that of the control group. Immunohistochemical examination showed minimum apoptotic changes in subgroup II as compared to subgroup I and nearly comparable to that of the control group. vitamin E showed an apparent protective role against the histological and apoptotic alterations caused by the toxic effect of AgNPs on both tongue papillae.
1. Ali M.A., Rehman I., Iqbal A., Din S., Rao A.Q., Latif A., Samiullah T.R., Azam S., Husnain T., 2014. Nanotechnology, a new frontier in Agriculture. International Journal of Advancements in Life Sciences. 1(3), 129-138.
2. Stankic S., Suman S., Haque F., Vidic J., 2016. Pure and multi metal oxide nanoparticles: synthesis, antibacterial and cytotoxic properties. Journal of Nanobiotechnology. 14(73), 1-20.
3. Uno M., Kurachi M., Wakamatsu N., Doi Y., 2013. Effects of adding silver nanoparticles on the toughening of dental porcelain. The Journal of Prosthetic Dentistry. 109(4), 241–247.
4. Stensberg M.C., Wei Q., McLamore E.S., Porterfield D.M., Wei A., Sepúlveda M.S., 2011. Toxicological studies on silver nanoparticles: challenges and opportunities in assessment, monitoring and imaging. Nanomedicine (London, England). 6(5), 879–898.
5. Sambale F., Wagner S., Stahl F., Khaydarov R.R., Scheper T., Bahnemann D., 2015. Investigations of the toxic effect of silver nanoparticles on mammalian cell lines. Journal of Nanomaterials. 10, 1-9.
6. Silva G.Á.F., Nunes R.A.L., Morale M.G., Boccardo E., Aguayo F., Termini L., 2018. Oxidative stress: therapeutic approaches for cervical cancer treatment. Clinics. 73, 1-10.
7. Lobo V., Patil A., Phatak A., Chandra N., 2010. Free radicals, antioxidants and functional foods: Impact on human health. Pharmacognosy Reviews. 4(8), 118-126.
8. Zhang Z., Shen W., Xue J., Liu Y., Liu Y., Yan P., Liu J., Tang J., 2018. Recent advances in synthetic methods and applications of silver nanostructures. Nanoscale Research Letter. 10, 13-54.
9. El Mahdy M.M., Eldinb T.A.S., Aly H.S., Mohammed F.F., Shaalana M.I., 2014. Evaluation of hepatotoxic and genotoxic potential of silver nanoparticles in albino rats. Experimental and Toxicologic Pathology. 67, 21–29.
10. Bashandy S.A.E., 2006. Beneficial Effect of Combined Administration of Vitamin C and Vitamin E in Amelioration of Chronic Lead Hepatotoxicity. The Egyptian Journal of Hospital Medicine. 23, 371 – 384.
11. Asha Rani P.V.K., Mun G.L.K., Hande M.P., Valiyaveettil S., 2009. Cytotoxicity and genotoxicity of silver nanoparticles in human cells. ACS Nano. 3(2), 279–290.
12. Haase A., Rott S., Mantion A., Graf P., Plend J., Thünemann A.F., Meier W.P., Taubert A., Luch A., Reiser G., 2012. Effects of Silver Nanoparticles on Primary Mixed Neural Cell Cultures: Uptake, Oxidative Stress and Acute Calcium Responses. Toxicological Sciences. 126(2), 457–468.
13. Ahmad J., Alhadlaq H.A., Siddiqui M.A., Saquib Q., Al-Khedhairy A.A., Musarrat J., Ahamed M., 2013. Concentration-dependent induction of reactive oxygen species, cell cycle arrest and apoptosis in human liver cells after nickel nanoparticles exposure. J Environment Toxicology. 30(2), 137-148.
14. Shamel M., Al Ankily M., Bakr M., 2017. Epidermal growth factor restores normal levels of myosin expression in submandibular salivary glands of rats treated with botulinum toxin. Journal of Advanced Medical and Dental Sciences Research. 5(1), 1-6.
15. Loghman A., Iraj S.H., Naghi D.A., Pejman M., 2012. Histopathologic and apoptotic effect of nanosilver in liver of broiler chickens. African Journal of Biotechnology. 11(22), 6207–6211.
16. Liu X., Lee P.Y., Ho C.M., Lui V.C., Chen Y., Che C.M., Tam P.K., Wong K.K., 2010. Silver nanoparticles mediate differential responses in keratinocytes and fibroblasts during skin wound healing. Chem Med Chem. 5, 468-475.
17. Wang J., Sun P., Bao Y., Dou B., Song D., Li Y., 2012. Vitamin E renders protection to PC12 cells against oxidative damage and apoptosis induced by single-walled carbon nanotubes. Toxicology In Vitro. 26, 32–41.
18. Kurutas E.B., 2016. The importance of antioxidants which play the role in cellular response against oxidative/nitrosative stress: current state. Nutrition journal. 15(1), 71.
19. Zaki N., Al Ankily M., Amin R., Halawa A., 2020. Assessment of Silver Nanoparticles Toxicity and The Possible Protective Effect of administration of Vitamin C on Filiform and Circumvallate Tongue Papillae of Albino rats Histological and Immunohistochemically study. Egyptian Dental Journal. 66(1), 293-300.
20. Gopinath P., Gogoi S.K., Sanpui P., Paul A., Chattopadhyay A., Ghosh A.S., 2010. Signaling gene cascade in silver nanoparticle induced apoptosis. Colloids and Surfaces B: Biointerfaces. 77, 240–245.
21. Onoda A., Umezawa M., Ktakeda K., 2017. Developmental Toxicity of Nanoparticles on the Brain. Yakugaku Zasshi. 137(1), 73-78.
22. Teodoro J.S., Silva R., Varela A.T., Duarte F.V., Rolo A.P., Hussain S., Palmeira C.M., 2016. Low-dose, subchronic exposure to silver nanoparticles causes mitochondrial alterations in Sprague–Dawley rats. Nanomedicine. 11(11), 1359-1375.
23. Liu J., Chen H., Chang Q., Liu X., Wu Y., Wei C., Li R., Kwan J. K., Yeung K.L., Xi Z., Lu Z., Yang X., 2014. Application of vitamin E to antagonize SWCNTs-induced exacerbation of allergic asthma. Scientific Report. 4, 42-75.
