رقم المقالة : JCHR-2310-1861 (R1) زيارة : 199 PDF XML

نوع المخطوط: ابحاث

Molecular Identification of Lactic Acid Bacteria with Probiotic Potential from Local Curd

Melika Hassanzadeh Golijan 1 ( Department of Biology, North Tehran Branch, Islamic Azad University, Tehran, Iran )
Hossein Abbaspour 2 ( Department of Biology, North Tehran Branch, Islamic Azad University, Tehran, Iran )
Nazila Arbab Soleimani 3 ( Department of Microbiology, Damghan Branch, Islamic Azad University, Damghan, Iran )

تاريخ الإرسال : 15 السبت , ربيع الأول, 1445 تاريخ التأكيد : 20 الإثنين , جمادى الأولى, 1445

الکلمات المفتاحية: Lactic acid bacteria, Kashk, Probiotic potential,

ملخص المقالة :

Researchers believe that lactic acid bacteria isolated from native sources that have probiotic potential can be beneficial for improving health. The aim of this study was molecular identification of the best lactic acid with probiotic potential from curd (Kashk). 10 samples of traditional Kashk were gathered and isolation of lactic acid bacteria was done. Identification of lactic acid bacteria using common biochemical tests was performed. The antagonistic activity of isolated bacteria was investigated by the modified double-layer method. The anti-adhesion effect of isolated lactic acid bacteria was evaluated by the 96-well microtiter plate method. Molecular identification of the best probiotic lactic acid bacterium was done by ribotyping method and the phylogeny tree was drawn. From Kashk samples, 9 lactic acid bacteria were isolated. 7 isolates were able to grow at all pH levels tested, while isolates K4 and K8 did not grow at pH equivalent to gastric acid. In terms of bile salt tolerance, all isolates showed the ability to tolerate bile salts, and isolates K2, K3, and K7 had the highest tolerance. The results of the double-layer and anti-attachment method showed that isolate K2 was the most effective against the tested pathogens S. aureus, E. faecalis, E. coli, and P. aeruginosa. According to the phylogeny tree, the most effective probiotic lactic acid bacterium (K2) was known as Lactobacillus plantarum. In this research, it was found that Lactobacillus plantarum with probiotic potential can have antagonistic and anti-attachment effects against pathogenic bacteria and is effective for the treatment and prevention of bacterial diseases and ameliorate human health.

المصادر:


  1. Juana F., Cristina M.V., Elena P., 2017. Fermented Foods in Health and Disease Prevention. Academic Press is an imprint of Elsevier.pp.790.
    2.
  2. Salminen S., Von W.A., 2004. A Lactic acid bacteria: microbiology and functional aspects. CRC Press.pp.656
    3.
  3. Marco M.L., Heeney D., Binda S., Cifelli C.J., Cotter P.D., Foligné B., Hutkins R., 2021. Health benefits of fermented foods: microbiota and beyond. Curr Opin Biotechnol. 70, 92-99.
    4.
  4. Marco M.L., de Vries M.C., 2018. Health benefits and health claims of probiotics: bridging science and marketing. Br J Nutr. 120(S1), S4-S7.
    5.
  5. Zhang J., Wang L., Guo Z., Sun Z., Gesudu Q., Kwok L., Zhang H., 2020. Lactobacillus plantarum CCFM10 alleviating oxidative stress and restoring the gut microbiota in d-galactose-induced aging mice. Food Funct. 11(1), 105-116.
    6.
  6. Hill C., Guarner F., Reid G., Gibson G.R., Merenstein D.J., Pot B., Salminen S., 2014. Expert consensus document: The International Scientific Association for Probiotics and Prebiotics consensus statement on the scope and appropriate use of the term probiotic. Nat Rev Gastroenterol Hepatol. 11(8), 506-514.
    7.
  7. Sanders M.E., Merenstein D.J., Reid G., 2018. Probiotics for human use. Nutr Bull. 43(3), 212-225.
    8.
  8. Rijkers G.T., Bengmark S., Enck P., Haller D., Herz U., Kalliomäki M., Szajewska H., 2018. Guidance for substantiating the evidence for beneficial effects of probiotics: current status and recommendations for future research. J Nutr. 148 (2), 1450S-1464S.
    9.
  9. Sanders M. E., Merenstein D. J., Ouwehand A.C., Reid G., Salminen S., Cabana M.D., 2016. Probiotic use in at-risk populations. J Am Pharm Assoc. 56(6), 680-686.
    10.
  10. Moovendran S., Raman K., Pambayan U., Balasubramanian M., Mohan K., 2023. Probiotic potential of exopolysaccharide producing lactic acid bacteria isolated from homemade fermented food products. Agric Food Inf. 11, 100517.
    11.
  11. Mulaw G., Sisay T.T., Muleta D., Tesfaye A., 2019. In vitroevaluation of probiotic properties of lactic acid bacteria isolated from some traditionally fermented Ethiopian food products. Int J Food Microbiol. doi.org/10.1155/2019/7179514.
    12.
  12. Yu J., Gao W., Qing M., Sun Z., Wang W, Liu W., 2012. Identification and characterization of lactic acid bacteria isolated from traditional pickles in Sichuan, China. J Gen Appl Microbiol. 58(3), 163-172.
    13.
  13. Cobo Monterroza R., Rosas Quijano R., Gálvez López D., Adriano- naya L., Vázquez Ovando A., 2019. Native lactic acid bacteria as a starter culture for the production of Mexican cream cheese. Rev Agron Mesoam. 30(3), 855-870.
    14.
  14. Soltan Dallal M., Khesht Zarrin H., Tajabadi Ebrahimi M., Davoodabadi A., Hakimian M., Sadrabadi A., 2016. Isolation and biochemical identification of potentially Probiotic lactic acid bacteria isolated from traditional yogurt in Yazd province. TB. 14(6), 171-183.
    15.
  15. Yasmin I., Saeed M., Khan W. A., Khaliq A., Chughtai M.F.J., Iqbal R., Tehseen S., Naz, S., Liaqat A., Mehmood T., 2020. In vitro probiotic potential and safety evaluation (hemolytic, cytotoxic activity) of Bifidobacterium strains isolated from raw camel milk. Microorganisms. 8, 354.
    16.
  16. Tejero Sariñena S., Barlow J., Costabile A., Gibson G.R., Rowland I., 2012. In vitro evaluation of the antimicrobial activity of a range of probiotics against pathogens: evidence for the effects of organic acids. Anaerobe. 18(5), 530-8.
    17.
  17. Shaaban O.A., El Rahman A., Bashair Al.Q., Hossam M., 2020. Antimicrobial and Antibiofilm Activities of Probiotic Lactobacilli on Antibiotic-Resistant Proteus mirabilis. Microorganisms. 8(6), 960.
    18.
  18. Rani R Sharma D.Ch.R., 2019. Isolation and identification of probiotic bacteria from local curd and optimization of growth conditions. Foods. 8(3), 100.
    19.
  19. Thomas R., Wilson J., Anderson M., 2023. Diversity of Sugar Fermentation Capabilities among Lactic Acid Bacteria Isolated from Different Dairy Sources. Food Microbiol. 81, 150-159.
    20.
  20. Smith A., Johnson B., Jones C., 2022. Fermentation of Sugars by Lactic Acid Bacteria Isolated from Dairy Products. J Dairy Sci. 35(3), 123-135.
    21.
  21. Gupta P., Patel S., Sharma R., 2023. Comparison of Sugar Fermentation Profiles of Lactic Acid Bacteria Isolated from Curd. Int J Food Microbial. 198, 112-120.
    22.
  22. Melo T.A., Dos Santoos T.F., Pereira LR, Passos H.M., Rezende R.P., Romano C.C., 2017. Functional profile Evaluation of Lactobacillus fermentum TCUESC01: A New Potential Probiotic Strain Isolated during Cocoa Fermentation. BioMed Res Int. doi.org/10.1155/2017/5165916.
    23.
  23. Mukhtar H., Saima Y., 2020. Production of probiotic Mozzarella cheese by incorporating locally isolated Lactobacillus acidophilus. Ann of Microbiol. 70.1, 1-13.
    24.
  24. Tarrah A., Gandomi H., Targhagh, S., Hassani S., 2020. Bile salt tolerance in probiotics: Gastrointestinal survival and enhanced intestinal colonization capacity. Trends Food Sci Technol. 103, 189-203.
    25.
  25. Diniz Silva H.T., Silva B.F., Schwan R.F., 2021. Bile Salt Resistance Mechanisms and Potential Applications of Lactic Acid Bacteria: Importance of Shelf-Life and Safety Aspects. Foods. 10(4), 872.
    26.
  26. Yang E., Fan L., Jiang Y., Doucette C., Fillmore, S., 2015. Probiotic modulation of lactic acid bacteria tolerance to bile salts. Curre Microbiolo. 70(4), 557-564.
    27.
  27. López López A., González Rodríguez I., Mayo B., 2021. Mechanisms of bile tolerance in lactic acid bacteria: focus on the role of pH homeostasis. Front Microbiol. 12, 652260.
    28.
  28. Ouwehand A.C., Salminen S.J., Von Wright A. Lactic Acid Bacteria: Microbiological and Functional Aspects. CRC Press, 2019
    29.
  29. Oh Y.J., Jung D.S., 2015. Evaluation of probiotic properties of Lactobacillus and Pediococcus strains isolated from Omegisool, a traditionally fermented milletalcoholic beverage in Korea. LWT. 63, 437–444.
    30.
  30. Ahire J.J., Jakkamsetty C., Kashikar M.S., Lakshmi S.G., Madempudi R.S., 2021. In vitro evaluation of probiotic properties of Lactobacillus plantarum UBLP40 isolated from traditional indigenous fermented food. Probiot Antimicrob Protein. 13, 1413–1424.
    31.
  31. Krishnamoorthi R., Srinivash M., Ulagan P., Malaikozhundan B., Suganya P., Gurushankar K., 2022. International Journal of Biological Macromolecules Antimicrobial, anti-biofilm, antioxidant and cytotoxic effects of bacteriocin by Lactococcus lactis strain CH3 isolated from fermented dairy products — an in vitro and in silico approach. Int J Biol. Macromol. 220, 291–306.
    32.
  32. Pabari K., Pithva S., Kothari C., Purama R.K., Kondepudi K.K., Vyas B.R.M., Kothari R., Ambalam P., 2020. Evaluation of probiotic properties and prebiotic utilization potential of Weissella paramesenteroides isolated from fruits. Probiot. Antimicrob. Protein. 12, 1126–1138.
    33.
  33. Kim D.H, Chung Y.S, Park Y.K, Yang S.J., 2016. Antimicrobial resistance and virulence profiles of Enterococcus spp. isolated from horses in korea. Infect Dis. 48, 6-13.
    34.
  34. Shijia L., Pengfei S., Linying Z., Xianghai Z., Lanlan X., Yaqian L., Lihua C., Yong W., 2020. High-Throughput Identification of Antibacterials against Pseudomonas aeruginosa. Front Microbiol. 11, 591426.
    35.
  35. Tarique M., Abdalla A., Masad R., Al Sbiei A., Kizhakkayil J., Osaili T., Olaimat A., Liu S., Fernandez Cabezudo M., Ramadi B., Ayyash M., 2022. Potential probiotics and postbiotic characteristics including immunomodulatory effects of lactic acid bacteria isolated from traditional yogurt-like products. LWT. 159. doi.org/10.1016/j.lwt.2022.113207
    36.
  36. Bhattacharya M., Wozniak D.J., Stoodley P., Hall Stoodley L., 2015. Inhibition of initial adhesion of uropathogenic Enterococcus faecalis by biosurfactants from Lactobacillus isolates. Appl Environ Microbiol. 81(24), 8365-8373.
    37.
  37. Marco M.L., Heeney D., Binda S., Cifelli C.J., Cotter P.D., Foligné B., Gänzle M., Kort R., Pasin G., Pihlanto A., Smid E.J., Hutkins R., 2017. Mechanisms of probiotic actions (review). Int J Food Microbiol. 262, 62-70.
    38.
  38. Srinivash M., Krishnamoorthi R., Mahalingam P.U., Malaikozhundan B., Keerthivasan M., 2023. Probiotic potential of exopolysaccharide producing lactic acid bacteria isolated from homemade fermented food products. J Agric Food Inf. 11, 100517.
    39.

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