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  • بررسی زیست سازگاری داربست PLA پوشش داده شده با لاپونیت بر روی سلول های بنیادی مزانشیمی مغز استخوان انسان

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رقم المقالة : ASCIJ-2103-1239 (R1) زيارة : 406 الصفحة: 101 - 117

نوع المخطوط: ابحاث

بررسی زیست سازگاری داربست PLA پوشش داده شده با لاپونیت بر روی سلول های بنیادی مزانشیمی مغز استخوان انسان

الموضوعات : فصلنامه زیست شناسی جانوری

زهرا عرفا 1 , شیوا ایرانی 2 , علی زمانیان 3 , هادی بخشی 4 , حبیب نیکوکار 5 , به آفرید قلندری 6

1 - گروه زیست شناسی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران
2 - گروه زیست شناسی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران
3 - گروه بیومواد، پژوهشکده فناوری نانو و مواد پیشرفته، پژوهشگاه مواد و انرژی، کرج، ایران
4 - پژوهشکده سیستم‌های پلیمری عامل، موسسه فرانهوفر برای تحقیقات پلیمری کاربردی، پوتسدام، آلمان
5 - مرکز تحقیقات نانو تکنولوژی پزشکی و مهندسی بافت، پژوهشکده علوم تولید مثل یزد، دانشگاه علوم پزشکی و خدمات بهداشتی درمانی شهید صدوقی یزد، یزد، ایران
6 - گروه نانوتکنولوژی پزشکی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران

تاريخ الإرسال : 24 السبت , جمادى الثانية, 1442 تاريخ التأكيد : 16 الخميس , شعبان, 1441 تاريخ الإصدار : 15 الإثنين , محرم, 1443

الکلمات المفتاحية: مهندسی بافت, الکتروریسی, استخوان, پلی‌لاکتیک اسید, لاپونیت,

ملخص المقالة :

مهندسی بافت استخوان رویکردی امیدوارانه جهت توسعه درمان های مناسب جدید برای رفع آسیب های بافت استخوانی است. یکی از اهداف مهم در این رشته، ساخت داربست هایی با تقلید از ماتریکس خارج سلولی است. هدف از این مطالعه تولید داربست پلی لاکتیک اسید/لاپونیت (PLA/LAP) و بررسی رفتار سلول های بنیادی مزانشیمی مغز استخوان انسانی (hBMSCs) بر روی آن بود. ابتدا داربست PLA به روش الکتروریسی ساخته شد و سپس LAP با غلظت 8/0 درصد وزنی (LAP0.8%) بر روی آن پوشش داده شد. مورفولوژی داربست توسط میکروسکوپ الکترونی روبشی (SEM) و طیف سنجی پراش انرژی پرتو ایکس (EDX)، ساختار شیمیایی آن توسط طیف سنجی ATR-FTIR و میزان آبدوستی داربست با آزمون زاویه تماس آب بررسی شد. نهایتاً زیست سازگاری داربست و بقای سلولی توسط تست MTT، بر روی سلول های hBMSC انجام شد. نتایج حاصل از مورفولوژی داربست نشان دهنده پوشش دهی موفق LAP0.8% بر روی داربست PLA بود. همچنین، سطح آبدوستی داربست PLA پس از پوشش دهی با LAP بهبود یافت. زیست سازگاری داربست تا 24 ساعت بعد از کشت سلولی و بقای سلول های hBMSC تا 72 ساعت پس از کشت (001/0 ≥ p ) تایید شد. از نتایج بدست آمده در این تحقیق به نظر می رسد که داربست PLA/LAP0.8%بدلیل حفظ زیست سازگاری و بقای سلولی که بواسطه حضور یون های موجود در نانوذره LAP است، می تواند کاندیدای مناسبی برای برنامه های مهندسی بافت استخوان باشد.

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