ترکیبات فیتوشیمیایی، فعاليت آنتی‌اکسیدانی و اثرات ضدسرطاني عصاره ریشه و جوانه کینوا (Chenopodium quinoa) بر رده AGS سرطان معده
الموضوعات :
فاطمه شیرعلی
1
,
سیّد ابراهیم سیفتی
2
1 - گروه مدیریت مناطق خشک و بیابانی، دانشکده منابع طبیعی و کویرشناسی، دانشگاه یزد، یزد، ایران
2 - مدیریت مناطق خشک و بیابانی ، منابع طبیعی و کویرشناسی ، دولتی یزد ، ایران
الکلمات المفتاحية: اثرات سیتوتوکسیکی, آزمون سمیت MTT, تیتیکاکا, فلاونوئید, ساپونین,
ملخص المقالة :
به منظور بررسی ترکیبات فیتوشیمیایی، فعالیت آنتی‌اکسیدانی و اثر ضدسرطانی ریشه و جوانه ارقام مولتی‌هد بالک، بلک و تیتیکاکا بر رده سلولی آدنوکارسینوم (AGS) سرطان معده، ارقام در قالب طرح بلوک¬های کامل تصادفی با 4 تکرار در مزرعه مناطق خشك و نيمه‌خشک ایران در دانشگاه یزد کشت شدند. نمونه‌های ریشه، قبل از پرشدن دانه و از عمق 30 سانتی‌متری خاک برداشت شد. از هر رقم، پس از کامل شدن مرحله رشد زایشی و پر شدن دانه، 100 گرم بذر به طور تصادفی جهت تولید جوانه بدون این که پوست‌گیری شود، برداشت گردید. بیشترین میزان ساپونین بر حسب میلی‌گرم بر گرم وزن خشک نمونه، در ریشه بلک (24/4) و سپس ریشه تیتیکاکا (7/1) و کمترین میزان آن (6/0) در جوانه بلک مشاهده شد. محتوای فیتوشیمیایی و آنتی‌اکسیدانی عصاره هیدرواتانولی (80:20) به روش سوکسله ریشه و جوانه هر سه رقم، در غلظت‌هاي 100، 250، 500 و 1000 ميلي‌گرم بر ميليليتر بررسی گردید. بر این اساس، به ترتیب عصاره جوانه ارقام بلک و تیتیکاکا در غلظت 1000 میلی‌گرم بر میلی‌لیتر از نظر محتوای كل تركيبات فنولی (68/172 میلی‌گرم گالیک اسید به گرم ماده خشک)، فلاونوئيدي (17/128 میلی‌گرم کوئرستین به گرم ماده خشک) و ظرفيت آنتی‌اکسیدانی، نسبت به نمونه‌های متناظر در رقم مولتی‌هد بالک و همچنین نمونه ریشه هر سه رقم، برتری معنی‌داری داشتند. کمترین و بیشترین DPPH IC50 به ترتیب در عصاره جوانه بلک (11/1 میلی‌گرم بر میلی‌لیتر) و ریشه مولتی‌هد بالک (15/3) مشاهده شد. كمترين درصد زنده‌ماني سلول‌های رده AGS به ترتيب در غلظت 1000 ميلي‌گرم بر ميليليتر عصاره جوانه بلك (46/33) و تيتيكاكا (11/36) در زمان 72 ساعت بود. نتایج این مطالعه پس از بررسی خصوصیات فیتوشیمیایی و آنتی‌اکسیدانی جوانه در مقایسه با بذر ارقام مورد مطالعه در دو حالت پوست‌گیری شده و دارای پوست، می‌تواند منجر به توسعه و تسهیل دسترسی بیماران مبتلا به سرطان به این گیاه دارویی شود.
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