مطالعه تاثيراکسین و جیبرلین بر رشد، محتوای کلروفیلی، کاروتنوئیدی و ترپنوئیدی کل در گیاه افوربیا تریگونا (Euphorbia trigona Mill.)
الموضوعات :
حکیمه رضایی
1
(گروه زیست شناسی گیاهی ، دانشکده علوم پایه، واحد گرگان، دانشگاه آزاد اسلامی، گرگان، ایران، )
آرین ساطعی
2
(گروه زیست‌شناسی، واحد گرگان، دانشگاه آزاد اسلامی، گرگان، ایران،)
طاهره سادات آقاجانزاده
3
(گروه علوم گیاهی، دانشکده علوم، دانشگاه مازندران، بابلسر، ایران، )
مهدی عبادی
4
(رییس مرکز تحقیقات آنالیز ترکیبات شیمیایی)
الکلمات المفتاحية: افوربیا تریگونا, اکسین, ایندول بوتیریک اسید, بیس 2-اتیل¬هگزیل فتالات, ترپنوئید, ژیبرلین, کاروتنوئید, کلروفیل, گازکروماتوگرافی-اسپکترومتری¬جرمی, نفتالن استیک اسید,
ملخص المقالة :
این پژوهش به‌منظور مطالعه تاثيراکسین و جیبرلین بر گیاه افوربیا تریگونا در قالب طرح بلوك‌هاي كامل تصادفي در سال 1399 در شرایط گلخانه‌ای به اجرا درآمد. پیش تيمارهایی یک دقیقه‌ای شامل تنظيم‌كننده‌هاي اكسيني نفتالن¬استیک¬اسید، ایندول¬بوتیریک¬اسید و ایندول استیک‌اسید با غللظت ۵۰۰ میلی‌گرم در لیتر و تيمارهایی شامل هورمون‌های ژیبرلین، ایندول استیک¬اسید، ایندول¬بوتیریک¬اسید و نفتالن¬استیک¬اسید در غلظت ۲۵۰ میلی‌گرم در لیتر در مقایسه با هم و با گیاهان شاهد به کار گرفته شدند. وزن تر و خشک و درصد وزن خشک ساقه و ریشه، محتوای کلی رنگیزه‌های فتوسنتزی و مقدار ترپنوئید‌های کل اندازه گیری شد. وزن تر و خشک و درصد وزن خشک ساقه نسبت به تیمارها و پیش تیمارهای به کار رفته حساسیتی نشان نداد و تاثیرات معنی‌دار نبود. تیمار نفتالن ¬استیک¬ اسید بیشترین اثر مثبت را در افزایش وزن تر و خشک ریشه داشت هر چند درصد وزن خشک ریشه تحت تاثیرات معنی‌دار تیماری قرار نگرفت. بیشترین افزایش معنی‌دار در محتوای ترپنوئیدی در تیمار نفتالن¬استیک ¬اسید و پیش تیمار نفتالن¬استیک اسید و ایندول¬بوتیریک¬اسید مشاهده شد. تیمار ژیبرلین بیشترین اثرات مثبت را بر محتوای هر یک از کلروفیل‌های a و b، مجموع آنها و نیز محتوای کاروتنوئیدی داشت. از نظر تاثیر بر ترکیب آنتن فتوسنتزی نیز بیشترین تاثیرات مثبت بر نسبت کلروفیل کل به کاروتنوئید در تیمارهای ژیبرلین، نفتالن¬استیک¬اسید، ایندول¬استیک¬اسید و پیش تیمار ایندول¬استیک¬اسید ملاحظه شد. همچنین تیمار ایندول¬استیک¬اسید افزایش معنی‌داری را در نسبت کلروفیل a به b نشان داد. همبستگی بین پارامترهای رشد با یکدیگر و نیز همبستگی محتوای کلروفیل‌ها با یکدیگر و با کاروتنوئیدها مثبت و معن یدار بود. با اینحال همبستگی محتوای ترپنوئیدی با هیچیک از موارد دیگر معنی دار نبود. از سوی دیگر و از نقطه نظر کاربردی، بر اساس اندازه‌گیری‌های کروماتوگرافی گازی-طیف‌سنجی جرمی، تیمار نفتالین-استیک-اسید در افزایش شدید محتوای ترکیب دارویی ارزشمند بیس-2-اتیل هگزیل فتالات نسبت به شاهد موثر بود.
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