The Effects of Dietary Inclusion of Arginine and Rice Hull on Growth Performance, Immune Responses, Intestinal Morphology, Tibia Bone Mineral Content and Intestinal Microbial Population of Broiler Chickens
الموضوعات :M. Abbasabadi 1 , S.M. Hosseini 2 , H. Naeimipour 3 , F. Izadi Yazdanabadi 4
1 - Department of Animal Science, Faculty of Agriculture, University of Birjand, Birjand, Iran
2 - Department of Animal Science, Faculty of Agriculture, University of Birjand, Birjand, Iran
3 - Department of Animal Science, Faculty of Agriculture, University of Birjand, Birjand, Iran
4 - Department of Animal Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
الکلمات المفتاحية: arginine, body weight, broiler chickens, fiber, gastrointestinal tract,
ملخص المقالة :
Arginine and fibers may have positive effects on gastrointestinal tract and indirectly affect immune re-sponses, growth performance, intestinal morphology and microbial population. This study was conducted to evaluate the effects of dietary inclusion of arginine and rice hull on growth performance, immune re-sponses, intestinal morphology and intestinal microbial population of broiler chickens. Three hundred and sixty male broiler chickens were randomly allocated into nine dietary treatments with four replications of 10 broiler chickens based on a factorial arrangement (3×3) in a completely randomized design. Experimen-tal treatments were including basal diets supplemented with arginine (90, 100, and 110% recommended levels), and fiber (0.00, 2.50, and 5.00%). Growth performance, immune responses, intestinal morphology, tibia bone mineral content and intestinal microbial population of broiler chickens were measured. The ef-fects of dietary inclusion of rice hull, arginine, and their interactions were significant on average daily gain (P<0.05) and feed conversion ratio (FCR) (P<0.05), so that highest gain and the lowest feed conversion ratio were observed in broiler chickens fed with highest levels of arginine (110.00%) and fiber (5.00%). Dietary supplementation of arginine progressively increased immune responses (P<0.05), while inclusion of rice hull increased villus height and crypt depth (P<0.05) and decreased the concentrations of calcium (P<0.05), phosphorous (P<0.05) and magnesium (P<0.05). The inclusion of arginine (P<0.05), and fiber (P<0.05) in higher levels increased Lactobacilli population (P<0.05). In total, higher levels of fiber (5.00%) and arginine (110.00%) improved the growth performance and are suggested in diet of broiler chickens for improving growth performance.
Abazari A., Navidshad B., Mirzaei Aghjehgheshlagh F. and Nikbin S. (2016). The effect of rice husk as an insoluble dietary fiber source on intestinal morphology and Lactobacilli and Escherichia coli populations in broilers. Iranian J. Vet. Med. 10, 217-24.
Aguzey H.A., Gao Z., Haohao W., Guilan C., Zhengmin W., Junhong C. and Li N.Z. (2020). The role of arginine in disease prevention, gut microbiota modulation, growth performance and the immune system of broiler chicken: A review. Ann. Anim. Sci. 20, 325-341.
Allen P.C. and Fetterer R.H. (2002). Recent advances in biology and immunobiology of Eimeria species and in diagnosis and control of infection with these coccidian parasites of poultry. Clin. Microbiol. Rev. 15, 58-65.
Aviagen. (2014). Ross 308: Broiler Nutrition Specification. Aviagen Ltd., Newbridge, UK.
D’Amato J.L. and Humphrey B.D. (2010). Dietary arginine levels alter markers of arginine utilization in peripheral blood mononuclear cells and thymocytes in young broiler chickens1. Poult. Sci. 89, 938-947.
David L.A., Maurice C.F., Carmody R.N., Gootenberg D.B., Button J.E., Wolfe B.E., Ling A.V., Devlin A.S., Varma Y. and Fischbach M.A. (2014). Diet rapidly and reproducibly alters the human gut microbiome. Nature. 505, 559-563.
Davila D., Brief S., Simon J., Hammer R.E., Brinster R. and Kelley K. (1987). Role of growth hormone in regulating T Dependent immune events in aged, nude, and transgenic rodents. J. Neurosci. Res. 18, 108-116.
Debon S.J. and Tester R.F. (2001). In vitro binding of calcium, iron and zinc by non-starch polysaccharides. Food Chem. 73, 401-110.
Emadi M., Jahanshiri F., Kaveh K., Hair-Bejo M., Ideris A. and Alimon A.R. (2011). Nutrition and immunity: the effects of the combination of arginine and tryptophan on growth performance, serum parameters and immune response in broiler chickens challenged with infectious bursal disease vaccine. Avian Pathol. 40, 63-72.
Ghamari Monavvar H., Moghaddam G. and Ebrahimi M. (2020). A review on the effect of arginine on growth performance, meat quality, intestine morphology, and immune system of broiler chickens. Iranian J. Appl. Anim. Sci. 10(4), 587-594.
Guo Y., Shi B., Yan S., Xu Y., Li J. and Li T. (2015). Effects of arginine on cytokines and nitric oxide synthesis in broilers. J. Anim. Plant Sci. 25, 366-371.
Hartini S. and Purwaningsih P. (2018). Effects of adding insoluble non-starch polysaccharides and exogenous enzymes to a commercial broiler diet on the growth performance and carcass weight of broiler chickens. Pakistan J. Nutr. 16(4), 227-235.
Hartini S., Rahardjo D.D. and Purwaningsih P. (2019). The Effects of rice hull inclusion and enzyme supplementation on the growth performance, digestive traits, dry matter and phosphorus content of intestinal digesta and feces of broiler chickens. Int. J. Poult. Sci. 18, 21-27.
He S., Yin Q., Xiong Y., Liu D. and Hu H. (2020). Effects of dietary fumaric acid on the growth performance, immune response, relative weight and antioxidant status of immune organs in broilers exposed to chronic heat stress. Czech J. Anim. Sci. 65, 104-113.
Hu C., Li F., Duan Y., Yin Y. and Kong X. (2019). Dietary supplementation with leucine or in combination with arginine decreases body fat weight and alters gut microbiota composition in finishing pigs. Front. Microbiol. 10, 1767-1777.
Jiménez-Moreno E., de Coca-Sinova A., González-Alvarado J.M. and Mateos G.G. (2016). Inclusion of insoluble fiber sources in mash or pellet diets for young broilers. 1. Effects on growth performance and water intake. Poult. Sci. 95, 41-52.
Li P., Yin Y.L., Li D., Kim S.W. and Wu G. (2007). Amino acids and immune function. Br. J. Nutr. 98, 237-352.
Maghsoudi A., Vaziri E., Feizabadi M. and Mehri M. (2020). Fifty years of sheep red blood cells to monitor humoral immunity in poultry: a scientometric evaluation. Poult. Sci. 99(10), 4758-4768.
Montagne L., Pluske J.R. and Hampson D.J. (2003). A review of interactions between dietary fibre and the intestinal mucosa, and their consequences on digestive health in young non-ruminant animals. Anim. Feed Sci. Technol. 108, 95-117.
Moyo S., Jaja I.F., Mopipi K., Masika P. and Muchenje V. (2021). Effect of graded levels of Imbrasia belina meal on blood lipid profile, bone morphometric and mineral content of broiler chickens. Anim. Feed Sci. Technol. 271, 114736-114746.
Nakhon S., Numthuam S., Charoensook R., Tartrakoon W., Incharoen P. and Incharoen T. (2019). Growth performance, meat quality, and bone-breaking strength in broilers fed dietary rice hull silicon. Anim. Nutr. 5(2), 152-155.
Perez-Carbajal C., Caldwell D., Farnell M., Stringfellow K., Pohl S., Casco G., Pro-Martinez A. and Ruiz-Feria C.A. (2010). Immune response of broiler chickens fed different levels of arginine and vitamin E to a coccidiosis vaccine and Eimeria challenge. Poult. Sci. 89, 1870-1877.
Ravindran V. (2016). Feed-induced specific ileal endogenous amino acid losses: Measurement and significance in the protein nutrition of monogastric animals. Anim. Feed Sci. Technol. 221, 304-313.
Ren W., Chen S., Yin J., Duan J., Li T., Liu G., Feng Z., Tan B., Yin Y. and Wu G. (2014). Dietary arginine supplementation of mice alters the microbial population and activates intestinal innate immunity. J. Nutr. 144, 988-995.
Rezaei M., Karimi Torshizi M.A.M. and Rouzbehan Y. (2011). The influence of different levels of micronized insoluble fiber on broiler performance and litter moisture. Poult. Sci. 90, 2008-2012.
Sabour S., Tabeidian S.A. and Sadeghi G. (2019). Dietary organic acid and fiber sources affect performance, intestinal morphology, immune responses and gut microflora in broilers. Anim. Nutr. 5, 156-162.
Sadeghi A., Toghyani M. and Gheisari A. (2015). Effect of various fiber types and choice feeding of fiber on performance, gut development, humoral immunity, and fiber preference in broiler chickens. Poult Sci. 94, 2734-43.
Teirlynck E., Bjerrum L., Eeckhaut V., Huygebaert G., Pasmans F., Haesebrouck F., Dewulf J., Ducatelle R. and Van Immerseel F. (2009). The cereal type in feed influences gut wall morphology and intestinal immune cell infiltration in broiler chickens. Br. J. Nutr. 102, 1453-1461.
Tremaroli V. and Bäckhed F. (2012). Functional interactions between the gut microbiota and host metabolism. Nature. 489, 242-249.
Van der Wielen P.W., Biesterveld S., Lipman L.J. and van Knapen F. (2001). Inhibition of a glucose-limited sequencing fed-batch culture of Salmonella enterica serovar enteritidis by volatile fatty acids representative of the ceca of broiler chickens. Appl. Environ. Microbiol. 67, 1979-1982.
Wils-Plotz E.L. and Dilger R.N. (2013). Combined dietary effects of supplemental threonine and purified fiber on growth performance and intestinal health of young chicks. Poult. Sci. 92, 726-734.
Yazdanabadi F.I., Mohebalian H., Moghaddam G., Abbasabadi M., Sarir H., Vashan S.J.H. and Haghparast A. (2020a). Influence of Eimeria spp. infection and dietary inclusion of arginine on intestine histological parameters, serum amino acid profile and ileal amino acids digestibility in broiler chicks. Vet. Parasitol. 286, 109241-109251.
Yazdanabadi F.I., Moghaddam G.H., Nematollahi A., Daghighkia H. and Sarir H. (2020b). Effect of arginine supplementation on growth performance, lipid profile, and inflammatory responses of broiler chicks challenged with coccidiosis. Prevent. Vet. Med. 180, 105031-105039.
Zhang B., Lv Z., Li H., Guo S., Liu D. and Guo Y. (2017). Dietary l-arginine inhibits intestinal Clostridium perfringens colonisation and attenuates intestinal mucosal injury in broiler chickens. Br. J. Nutr. 118, 321-332.
Zhang B., Lv Z., Li Z., Wang W., Li G. and Guo Y. (2018) Die-tary L-arginine supplementation alleviates the intestinal injury and modulates the gut microbiota in broiler chickens chal-lenged by Clostridium perfringens. Front. Microbiol. 9, 1716-1725.
