افزایش پاسخ رشد و فعالیت آنزیم آنتی اکسیدانی سینره (Pericallis × hybrida L.)
لی لی فاتح نژاد
1
(
بخش علوم باغبانی، دانشکده کشاورزی، دانشگاه شیراز. شیراز، ایران.
)
ابوالفضل جوکار
2
(
بخش علوم باغبانی، دانشکده کشاورزی، دانشگاه شیراز. شیراز، ایران.
)
الکلمات المفتاحية: فعالیت آنزیم های آنتی اکسیدانی, سینرره, pH بالای خاک, کلروز برگ, Pericallis × hybrida ,
ملخص المقالة :
سینرر (Pericallis hybrida L.) با مانع رشد و کلروز مواجه شده است. اسید هیومیک، سولفات آهن و کودهای کلات آهن برای کشت سینراریا در خاک قلیایی مورد بررسی قرار گرفتند. این پژوهش به صورت آزمایشی فاکتوریل در قالب طرح کاملاً تصادفی با سه تکرار و در هر تکرار 4 نمونه انجام شد. محیط‌های گیاهی با استفاده از اسید هیومیک (0، 0.5، 1 گرم بر کیلوگرم خاک) و کودهای آهن (0، 5 و 10 میلی‌گرم بر کیلوگرم سولفات آهن و 5 و 10 میلی‌گرم بر کیلوگرم کلات آهن) غنی‌سازی شدند. گیاهانی که با خاک حاوی 1 گرم در کیلوگرم اسید هیومیک همراه با 10 میلی گرم در کیلوگرم کلات آهن تیمار شدند، در ارتفاع بوته (86%)، قطر ساقه (100%)، وزن تر ریشه (170%)، دوره گلدهی(166%)، تعداد گل (182%)، تعداد گل آذین (252%)، قطر گل (59%) و میزان کلروفیل کل (300%) بهبود یافتند. کاربرد همزمان 1 گرم بر کیلوگرم اسید هیومیک و 10 میلی گرم بر کیلوگرم کلات آهن، میزان عناصر معدنی پتاسیم، نیتروژن و فسفر را به ترتیب 179، 193 و 675 درصد افزایش داد. ترکیبات 0.5 گرم بر کیلوگرم اسید هیومیک و 10 میلی گرم بر کیلوگرم کلات آهن باعث افزایش آنتوسیانین (131٪)، سطح برگ (140٪)، TSS (332٪) و نشاسته (642٪) شد. کوددهی با 0.5 گرم بر کیلوگرم اسید هیومیک در ترکیب با 5 میلی گرم بر کیلوگرم کلات آهن منجر به بیشترین فعالیت آنزیم های آنتی اکسیدانی SOD (238٪)، POD (324٪) و CAT (667٪) و کاهش نشت یونی (60%) شد. اسید هیومیک به عنوان یک کود زیستی در ترکیب با کلات آهن برای استفاده در تولید گیاهان در خاک هایی با شرایط PH بالا و استرس زا پیشنهاد می شود.
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