The Plant-Based Extender Supplemented with MitoQ as a Mitochondrial-Targeted Antioxidant Enhances Buck Sperm Quality during Liquid Storage
الموضوعات :M. Hatami 1 , R. Masoudi 2 , N. Asadzadeh 3 , H. Javaheri Barfourooshi 4
1 - Department of Animal Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
2 - Animal Science Research Institute of Iran (ASRI), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
3 - Animal Science Research Institute of Iran (ASRI), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
4 - Animal Science Research Institute of Iran (ASRI), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
الکلمات المفتاحية: buck, cooling, mitochondrial activity, MitoQ, sperm,
ملخص المقالة :
One of the problems with the refrigerated storage of buck semen is the short shelf life. Reactive oxygen species (ROS) generated by sperm cells are a major obstacle to successful cooled semen storage. In this regard, the mitochondria are the most sensitive organelles to cooling damage in sperm. Consequently, this study was aimed to determine the impacts of Mito-quinol (MitoQ(, a mitochondrial-targeted antioxidant, in a soy lecithin containing extender on the quality of goat semen during cooling and storage at 4 °C. Semen samples from Markhoz goats were collected and pooled. Then, samples were diluted in the extender and divided into five equal aliquots and supplemented with 0 (control), 1, 10, 100, and 1000 nM of MitoQ and stored at 4 °C for 60 h. Finally, total motility (TM) and progressive motility (PM), viability, membrane functionality, mitochondrial activity potential (MAP), and MDA concentration (lipid peroxidation) were measured at 0, 30, and 60 h of cooling period. According to the current results, higher (P≤0.05) rates of TM, PM, viability, membrane functionality, and MAP, as well as lower (P≤0.05) MDA concentration were observed in MQ10 and MQ100 groups than groups of MQ0, MQ1, and MQ1000 at 30 and 60 h cooling storage. However, MitoQ had no effect (P>0.05) on the mentioned parameters at 0 h storage. Consequently, MitoQ can reduce oxidative stress by regulating mitochondrial function during cooling of the buck sperm and could be an effective additive in the cooling medium to protect sperm quality.
Alcay S., Cakmak S., Cakmak I., Mulkpinar E., Gokce E., Ustuner B., Sen H. and Nur Z. (2019). Successful cryopreservation of honey bee drone spermatozoa with royal jelly supplemented extenders. Cryobiology. 87, 28-31.
Alipour-Jenaghard P., Daghigh-Kia H. and Masoudi R. (2023a). Preservation of the quality and fertility potential of post-thawed rooster sperm using MitoQ. Theriogenology. 208, 165-170.
Alipour-Jenaghard P., Daghigh-Kia H., Masoudi R., Moghaddam G. and Qasemi-Panahi B. (2023b). Mitochondria-targeted antioxidant “MitoQ” improves rooster’s cooled sperm quality indicators and reproductive performance. Theriogenology. 197, 26-30.
Arjun V., Kumar P., Dutt R., Kumar A., Bala R., Verma N., Jerome A., Virmani M., Patil C.S. and Bhardwaj S. (2022). Effect of mitochondria-targeted antioxidant on the regulation of the mitochondrial function of sperm during cryopreservation. Andrologia. 54, 14431-14439.
Asadzadeh N., Abdollahi Z., Esmaeilkhanian S. and Masoudi R. (2021). Fertility and flow cytometry evaluations of ram frozen semen in plant-based extender supplemented with Mito-TEMPO. Anim. Reprod. Sci. 233, 106-836.
Barfourooshi H.J., Asadzadeh N. and Masoudi R. (2023). The mitochondria-targeted antioxidant “MitoQ” preserves quality and reproductive performance of ram spermatozoa cryopreserved in soybean lecithin-based extender. Theriogenology. 208, 71-76.
Borges-Silva J.C., Silva M.R., Marinho D.B., Nogueira E., Sampaio D.C., Oliveira L.O.F., Abreu U.G.P., Mourao G.B. and Sartori R. (2016). Cooled semen for fixed-time artificial insemination in beef cattle. Reprod. Fertil. Dev. 28, 1004-1008.
Chelucci S., Pasciu V., Succu S., Addis D., Leoni G.G., Manca M.E., Naitana S. and Berlinguer F. (2015). Soybean lecithin–based extender preserves spermatozoa membrane integrity and fertilizing potential during goat semen cryopreservation. Theriogenology. 83, 1064-1074.
Escada-Rebelo S., Cristo M.I., Ramalho-Santos J. and Amaral S. (2022). Mitochondria-targeted compounds to assess and improve human sperm function. Antioxid. Redox Signal. 37, 451-480.
Esterbauer H. and Cheeseman K.H. (1990). Determination of aldehydic lipid peroxidation products: malonaldehyde and 4-hydroxynonenal. Methods Enzymol. 186, 407-421.
Fadl A.M., Abdelnaby E.A. and El-Sherbiny H.R. (2022). INRA82 extender enhances semen quality in ram under cooled and cryopreserved stages. Asian. Pac. J. Reprod. 11, 100-104.
Gualtieri R., Kalthur G., Barbato V., Di Nardo M., Adiga S.K. and Talevi R. (2021). Mitochondrial dysfunction and oxidative stress caused by cryopreservation in reproductive cells. Antioxidants. 10, 337-347.
Hatami M., Qasemi-panahi B., Daghigh Kia H., Moghaddam G. and Janmohammadi H. (2023). Egg yolk plasma enriched with β-carotene through the diet of laying hens and adding it to the extender improves the quality of frozen semen in Arabic stallions. Reprod. Domest. Anim. 58, 630-636.
Heidari M., Qasemi-Panahi B., Moghaddam G., Daghigh-Kia H. and Masoudi R. (2022). L-carnitine improves quality parame-ters and epigenetic patterns of buck’s frozen-thawed se-men. Anim. Reprod. Sci. 247, 107092-107099.
Ibrahim A.A., Karam H.M., Shaaban E.A., Safar M.M. and El-Yamany M.F. (2019). MitoQ ameliorates testicular damage induced by gamma irradiation in rats: modulation of mitochondrial apoptosis and steroidogenesis. Life. Sci. 232, 116-655.
Khodaei-Motlagh M., Masoudi R., Karimi-Sabet M.J. and Hatefi A. (2022). Supplementation of sperm cooling medium with Zinc and Zinc oxide nanoparticles preserves rooster sperm quality and fertility potential. Theriogenology. 183, 36-40
Kumar P., Wang M., Isachenko E., Rahimi G., Mallmann P., Wang W., Brandenstein M. and Isachenko V. (2021). Unraveling subcellular and ultrastructural changes during vitrification of human spermatozoa: Effect of a mitochondria-targeted antioxidant and a permeable cryoprotectant. Front. Cell Dev. Biol. 9, 672-862.
Layek S.S., Mohanty T.K., Kumaresan A. and Parks J.E. (2016). Cryopreservation of bull semen: Evolution from egg yolk based to soybean based extenders. Anim. Reprod. Sci.172, 1-9.
Leboeuf B., Delgadillo J.A., Manfredi E., Piacere A., Clément V., Martin P., Pellicer M., Boué P. and De Cremoux R. (2008). Management of goat reproduction and insemination for genetic improvement in France. Reprod. Domest. Anim. 43, 379-385.
Masoudi R., Asadzadeh N. and Sharafi M. (2020). The mitochondria-targeted antioxidant Mito-TEMPO conserves rooster’s cooled semen quality and fertility potential. Theriogenology. 156, 236-241.
Murphy C., Fahey A.G., Shafat A. and Fair S. (2013). Reducing sperm concentration is critical to limiting the oxidative stress challenge in liquid bull semen. J. Dairy Sci. 96, 4447-4454.
Nadri T., Towhidi A., Zeinoaldini S., Martínez-Pastor F., Mousavi M., Noei R., Tar M. and Sangcheshmeh A.M. (2019). Lecithin nanoparticles enhance the cryosurvival of caprine sperm. Theriogenology. 133, 38-44.
Peris-Frau P., Soler A.J., Iniesta-Cuerda M., Martín-Maestro A., Sánchez-Ajofrín I., Medina-Chávez D.A., Fernández-Santos M.R., García-Álvarez O., Maroto-Morales A. and Montoro V. (2020). Sperm cryodamage in ruminants: understanding the molecular changes induced by the cryopreservation process to optimize sperm quality. Int. J. Mol. Sci. 21, 27-81.
Revell S.G. and Mrode R.A. (1994). An osmotic resistance test for bovine semen. Anim. Reprod. Sci. 36, 77-86.
Rezaei A., Bahmani H.R., Mafakheri S., Farshad A., Nazari P. and Masoudi R. (2023). Protective effects of different doses of MitoQ separately and combined with trehalose on oxidative stress and sperm function of cryopreserved Markhoz goat semen. Cryobiology. 110, 36-43.
Ross M.F., Kelso G.F., Blaikie F.H., James A.M., Cocheme H.M., Filipovska A., Da Ros T., Hurd T.R., Smith R.A.J. and Murphy M.P. (2005). Lipophilic triphenylphosphonium cations as tools in mitochondrial bioenergetics and free radical biology. Biochemistry (Moscow). 70, 222-230.
Ross M.F., Prime T.A., Abakumova I., James A.M., Porteous C.M., Smith R.A.J. and Murphy M.P. (2008). Rapid and extensive uptake and activation of hydrophobic triphenylphosphonium cations within cells. Biochem. J. 411, 633-645.
Salmani H., Towhidi A., Zhandi M., Bahreini M. and Sharafi M. (2014). In vitro assessment of soybean lecithin and egg yolk based diluents for cryopreservation of goat semen. Cryobiology. 68, 276-280.
SAS Institute. (2003). SAS®/STAT Software, Release 9.1. SAS Institute, Inc., Cary, NC. USA.
Sun L., Fan W., Wu C., Zhang S., Dai J. and Zhang D. (2020). Effect of substituting different concentrations of soybean lecithin and egg yolk in tris-based extender on goat semen cryopreservation. Cryobiology. 92, 146-150.
Thun R., Hurtado M. and Janett F. (2002). Comparison of Biociphos-Plus® and TRIS-egg yolk extender for cryopreservation of bull semen. Theriogenology. 57, 1087-1094.
Tiwari S., Mohanty T.K., Bhakat M., Kumar N., Baithalu R.K., Nath S., Yadav H.P. and Dewry R.K. (2021). Comparative evidence support better antioxidant efficacy of mitochondrial-targeted (Mitoquinone) than cytosolic (Resveratrol) antioxidant in improving in-vitro sperm functions of cryopreserved buffalo (Bubalus bubalis) semen. Cryobiology. 101, 125-134.
Zhang Z., Liu Z., Zhang S., Lai C., Ma D. and Huang C. (2019). Effect of inclusion level of corn germ meal on the digestible and metabolizable energy and evaluation of ileal AA digestibility of corn germ meal fed to growing pigs. J. Anim. Sci. 97, 768-778.
