Investigating antitumorigenic effects of Vaccinium arctostaphylos on colorectal cancer cells

الموضوعات : Food and Health

Fatemeh Karami ¹ (Department of Medical Genetics, Applied Biophotonics Research Center, Science and Research Branch, Islamic Azad University, Tehran, Iran)
Mahsa Shahnazari ² (Department of Biotechnology and Plant Breeding, Faculty of Agriculture, Science and Research Branch of Islamic Azad University, Tehran, Iran)
Asa Ebrahimi ³ (Department of Biotechnology and Plant Breeding, Faculty of Agriculture, Science and Research Branch of Islamic Azad University, Tehran, Iran)
Mahmood Khosrowchahli ⁴ (Department of Biotechnology and Plant Breeding, Faculty of Agriculture, Science and Research Branch of Islamic Azad University, Tehran, Iran)

تاريخ الإرسال : 08 الثلاثاء , جمادى الثانية, 1443 تاريخ التأكيد : 29 الإثنين , شوال, 1443 تاريخ الإصدار : 02 الأربعاء , ذو القعدة, 1443

الکلمات المفتاحية: HCT-116 cell line /, Cytotoxicity /, <i>Vaccinium arctostaphylos</i> /, Polyphenol,

ملخص المقالة :

Anticancer and anti-inflammatory effects of American and European Cranberries have been previously shown on different cancer cells. Owing to the limited evidence on growth conditions and anticancer potentials of the Iranian Vaccinium genus, Vaccinium arctostaphylos, it was aimed to investigate its effect on colorectal cancer cells. In this regard, Vacciniumarctostaphylos was cultured in Woody Plant Medium (WPM) following incubation at 27°C in cycles of light and darkness. Callogenesis was induced using growth mediums containing different concentrations of 2,4-Dichlorophenoxyacetic acid (2,4-D), yeast extract, and Kinetin. Total polyphenol and antioxidant activity of prepared extracts from wet and dried callus and air-dried fruit was measured through the Folin-Ciocalteu method and DPPH assay, respectively. Various concentrations (0-500 µgr/ml) of fruit and callus extracts were examined on HCT-116 colorectal cancer cells. MTT assay was employed to determine the cytotoxicity of fruit and callus extracts. Obtained data were analyzed using Graph Pad Prism V7.04. The size and weight of the obtained callus were significantly dependent on the concentrations of 2,4-D, yeast extract, and Kinetin. Dry callus has been found to have the highest amount of polyphenol and antioxidant activity. HCT-116 cell death rate (20.5%) was demonstrated to be the most for dry callus at the concentration of 400 ‫‪µg/ml. However, half-maximal inhibitory concentration (IC50) was not achieved for none of Vaccinium arctostaphylos fruit or callus, Present evidence on cancer cell death can pave the way towards further assessment of anti-inflammatory and cancer cell cytotoxicity of the Iranian Cranberry genus.

المصادر:

1. Miller KD, Siegel RL, Lin CC, Mariotto AB, Kramer JL, Rowland JH, et al. Cancer treatment and survivorship statistics, 2016. CA: A Cancer Journal for Clinicians. 2016;66(4):271-89.


2. Yee NS. Update in systemic and targeted therapies in gastrointestinal oncology. Biomedicines. 2018;6(1):34.


3. Xie Y-H, Chen Y-X, Fang J-Y. Comprehensive review of targeted therapy for colorectal cancer. Signal Transduction and Targeted Therapy. 2020;5(1):22.


4. Papadimitriou N, Bouras E, van den Brandt PA, Muller DC, Papadopoulou A, Heath AK, et al. A prospective diet-wide association study for risk of colorectal cancer in EPIC. Clinical Gastroenterology and Hepatology. 2022;20(4):864-873.e13.


5. Thanikachalam K, Khan G. Colorectal cancer and nutrition. Nutrients. 2019;11(1):164


6. Van Blarigan EL, Zhang S, Ou FS, Venlo A, Ng K, Atreya C, et al. Association of diet quality with survival among people with metastatic colorectal cancer in the cancer and leukemia b and southwest oncology group 80405 trial. JAMA Network Open. 2020;3(10):e2023500.


7. Arora I, Sharma M, Tollefsbol TO. Combinatorial epigenetics impact of polyphenols and phytochemicals in cancer prevention and therapy. International Journal of Molecular Sciences. 2019;20(18):4567.


8. Biersack B. Current state of phenolic and terpenoidal dietary factors and natural products as non-coding RNA/microRNA modulators for improved cancer therapy and prevention. Non-coding RNA Research. 2016;1(1):12-34.


9. Boivin D, Blanchette M, Barrette S, Moghrabi A, Béliveau R. Inhibition of cancer cell proliferation and suppression of TNF-induced activation of NFkappaB by edible berry juice. Anticancer Research. 2007;27(2):937-48.


10. Denis MC, Desjardins Y, Furtos A, Marcil V, Dudonné S, Montoudis A, et al. Prevention of oxidative stress, inflammation and mitochondrial dysfunction in the intestine by different cranberry phenolic fractions. Clinical Science. 2015;128(3):197-212.


11. Kondo M, MacKinnon SL, Craft CC, Matchett MD, Hurta RA, Neto CC. Ursolic acid and its esters: occurrence in cranberries and other Vaccinium fruit and effects on matrix metalloproteinase activity in DU145 prostate tumor cells. Journal of the Science of Food and Agriculture. 2021;30;91(5):789-96.


12. Ferguson PJ, Kurowska EM, Freeman DJ, Chambers AF, Koropatnick J. In vivo inhibition of growth of human tumor lines by flavonoid fractions from cranberry extract. Nutrition and Cancer. 2006;56(1):86-94.


13. Vu KD, Carlettini H, Bouvet J, Côté J, Doyon G, Sylvain J-F, et al. Effect of different cranberry extracts and juices during cranberry juice processing on the antiproliferative activity against two colon cancer cell lines. Food Chemistry. 2012;132(2):959-67.


14. Asgary S, Soltani R, Mirvakili S, Sarrafzadegan N. Evaluation of the effect of Vaccinium arctostaphylos L. fruit extract on serum inflammatory biomarkers in adult hyperlipidemic patients: a randomized double-blind placebo-controlled clinical trial. Research in Pharmaceutical Sciences. 2016;11(4):343-8.


15. Gorbanzadeh E, Zaefizadeh M. Inhibitory effects of vaccinium arctostaphylos extract on prostate cancer cells. Journal of Ardabil University of Medical Sciences. 2017;17(4):487-96.


16. Mahboubi M, Kazempour N, Taghizadeh M. In vitro antimicrobial and antioxidant activity of Vaccinium arctostaphylos extracts. Journal of Biologically Active Products from Nature. 2013;3(4):241-7.


17. Karimian R, Lahouti M, Davarpanah SJ. Effects of different concentrations of 2, 4-d and kinetin on callogenesis of Taxus brevifolia Journal of Applied Biotechnology Reports. 2014;1(4):167-70.


18. Rashmi R, Trivedi MP. Effect of various growth hormone concentration and combination on callus induction, nature of callus and callogenic response of Nerium odorum. Applied Biochemistry and Biotechnology. 2014;172(5):2562-70.


19. Farjaminezhad R, Garoosi G. Improvement and prediction of secondary metabolites production under yeast extract elicitation of Azadirachta indica cell suspension culture using response surface methodology. AMB Express. 2021;11(1):43.


20. Chang C-H, Chiu H-F, Han Y-C, Chen IH, Shen Y-C, Venkatakrishnan K, et al. Photoprotective effects of cranberry juice and its various fractions against blue light-induced impairment in human retinal pigment epithelial cells. Pharmaceutical Biology. 2017;55(1):571-80.


21. Ziemlewska A, Zagórska-Dziok M, Nizioł-Łukaszewska Z. Assessment of cytotoxicity and antioxidant properties of berry leaves as by-products with potential application in cosmetic and pharmaceutical products. Scientific Reports. 2021;11(1):3240.


22. Michalska A, Wojdyło A, Honke J, Ciska E, Andlauer W. Drying-induced physico-chemical changes in cranberry products. Food Chemistry. 2018;240:448-55.


23. Vinson JA, Zubik L, Bose P, Samman N, Proch J. Dried fruits: excellent in vitro and in vivo antioxidants. Journal of the American College of Nutrition. 2005;24(1):44-50.


24. Miletj N, Popovi B, Mitrovj O, Kandic M, Leposavj A. Phenolic compounds and antioxidant capacity of dried and candied fruits commonly consumed in Serbia. Czech Journal of Food Sciences. 2018;32:360-8.


25. Jurikova T, Skrovankova S, Mlcek J, Balla S, Snopek L. Bioactive compounds, antioxidant activity, and biological effects of European cranberry (Vaccinium oxycoccos). Molecules. 2019;24(1):24.


26. Gudžinskaitė I, Stackevičienė E, Liaudanskas M, Zymonė K, Žvikas V, Viškelis J, et al. Variability in the qualitative and quantitative composition and content of phenolic compounds in the fruit of introduced American cranberry (Vaccinium macrocarpon Aiton). Plants. 2020;16;9(10):1379.


27. Jin D, Liu T, Dong W, Zhang Y, Wang S, Xie R, et al. Dietary feeding of freeze-dried whole cranberry inhibits intestinal tumor development in Apc(min/+) mice. 2017;8(58):97787-800.


28. Kraujalytė V, Venskutonis PR, Pukalskas A, Česonienė L, Daubaras R. Antioxidant properties and polyphenolic compositions of fruits from different European cranberrybush (Viburnum opulus) genotypes. Food Chemistry. 2013;141(4):3695-702.


29. Yu H, Kim D-J, Choi H-Y, Kim SM, Rahaman MI, Kim Y-H, et al. Prospective pharmacological methodology for establishing and evaluating anti-cancer drug resistant cell lines. BMC Cancer. 2021;21(1):1049.


30. Lefranc F, Tabanca N, Kiss R. Assessing the anticancer effects associated with food products and/or nutraceuticals using in vitro and in vivo preclinical development-related pharmacological tests. Seminars in Cancer Biology. 2017;46:14-32.