بررسی اثرات ژنوتوکسیک آرسنیک بر ماهی کلمه (Rutilus caspicus) با استفاده از آزمون های میکرونوکلئوس و کامت

الموضوعات :

مهسا دانشیان ¹ (کارشناس ارشد بوم‌شناسی آبزیان، دانشکده شیلات و محیط زیست، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، ایران.)
حدیثه کشیری ² (استادیار، دانشکده شیلات و محیط زیست، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، ایران. *(مسوول مکاتبات))
سید علی اکبر هدایتی ³ (دانشیار، دانشکده شیلات و محیط زیست، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، ایران.)
کاوه خسرویانی ⁴ (کارشناس ارشد شیلات، دانشکده منابع طبیعی کرج، دانشگاه تهران، ایران.)

تاريخ الإرسال : 08 الثلاثاء , ربيع الثاني, 1439 تاريخ التأكيد : 23 الأربعاء , شعبان, 1439 تاريخ الإصدار : 09 الأربعاء , رمضان, 1442

الکلمات المفتاحية: میکرونوکلئوس, ژنوتوکسیک, کامت, Rutilus caspicus, آرسنیک,

ملخص المقالة :

زمینه و هدف: آرسنیک به عنوان یک آلاینده می تواند منجر به بروز مشکلات جدی در آبزیان و انسان گردد. در پژوهش حاضر با توجه به ارزش اقتصادی و بوم شناختی ماهی کلمه دریای خزر (Rutilus caspicus)، به ارزیابی اثرات ژنوتوکسیک آرسنیک بر این گونه با استفاده از آزمون های کامت و میکرونوکلئوس در سال 1395 پرداخته شده است.روش بررسی: ابتدا ماهیان در معرض غلظت های 3، 6 و 9 میلی گرم در لیتر آرسنیک قرار گرفتند. یک تیمار شاهد نیز که فاقد آرسنیک بود، درنظر گرفته شد. نمونه برداری طی روزهای 3 و 7 مواجهه انجام گرفت. به منظور انجام تست میکرونوکلئوس، خون گیری طی دو نوبت انجام و فراوانی میکرونوکلئوس ها تعیین شد. برای انجام آزمون کامت از بافت آبشش ماهیان نمونه برداری و پارامترهای آسیب DNA ارزیابی شد.یافته ها: مواجهه با آرسنیک باعث القای بروز میکرونوکلئوس ها در اریتروسیت ها و آسیب به DNA در بافت آبشش گردید. به نحوی که فراوانی میکرونوکلئوس ها و مقادیر پارامترهای آسیب در تیمارهای مواجهه یافته با آرسنیک در مقایسه با شاهد به طور معنی داری افزایش یافت (05/0≥P). میزان آسیب وارد شده وابسته به زمان و غلظت مواجهه بود. بالاترین میزان پارامترهای آسیب شامل Tail Length، Tail Moment، %Tail DNA و همچنین فراوانی میکرونوکلئوس ها به ترتیب 55/1±1/101، 64/1±11/57، 09/3±45/59 و 58/0±26/2 بود که در تیمار مواجهه یافته با 9 میلی گرم در لیتر آرسنیک مشاهده شد.بحث و نتیجه گیری: نتایج پژوهش حاضر حاکی از آن است که غلظت های تحت کشنده آرسنیک می تواند باعث بروز میکرونوکلئوس ها و القای آسیب DNA در ماهی کلمه شود. لذا، می توان بیان داشت که آرسنیک دارای اثرات ژنوتوکسیک بر ماهی کلمه است.

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