سویه جدید ردوترولا موسیلاژینوزا تولیدکننده بیوسورفکتانت سوفورولیپید به عنوان یک عامل مؤثر در استخراج میکروبی نفت
الموضوعات :
زهرا گنجی
1
(دکتری، گروه میکروبیولوژی، دانشکده علوم زیستی، واحد فلاورجان، دانشگاه آزاد اسلامی، اصفهان، ایران.)
کیوان بهشتی مآل
2
(دانشیار، گروه میکروبیولوژی، دانشکده علوم زیستی، واحد فلاورجان، دانشگاه آزاد اسلامی، اصفهان، ایران)
احمدرضا مساح
3
(استاد، گروه شیمی، دانشکده علوم، واحد شهرضا، دانشگاه آزاد اسلامی، شهرضا، اصفهان، ایران.)
زرین دخت امامی کرونی
4
(استادیار، گروه میکروبیولوژی، دانشکده علوم زیستی، واحد فلاورجان، دانشگاه آزاد اسلامی، اصفهان، ایران.)
تاريخ الإرسال : 05 السبت , شعبان, 1444
تاريخ التأكيد : 10 الثلاثاء , ذو القعدة, 1444
تاريخ الإصدار : 01 الجمعة , شوال, 1444
الکلمات المفتاحية:
استخراج میکروبی نفت,
کشش سطحی,
شاخص امولسیون,
پراکنش روغن,
ردوترولا موسیلاژینوزا,
بیوسورفکتانت,
سوفورولیپید,
ملخص المقالة :
هدف: بیوسورفکتانت‌‌ها کاربردهای گسترده‌‌ای در میکروبیولوژی مواد غذایی و نفت دارند. هدف این تحقیق بررسی تولید بیوسورفکتانت پایدار در حرارت و شوری بالا از مخمرهای جداسازی شده از خاک آلوده به نفت بود.مواد و روش‌‌ها: از محیط کشت بوشنل هاس برای غربال‌گری مخمرهای مولد بیوسورفکتانت استفاده شد. وجود بیوسورفکتانت با استفاده از آزمون‌‌های پراکنش روغن و کاهش کشش سطحی ارزیابی شد.یافته‌ها: بهترین سویه تولیدکننده بیوسورفکتانت ردوترولا موسیلاژینوزا GBMEIAUF1 نام‌‌گذاری شد و توالی ژن 5.8s-rDNA آن در بانک ژنی NCBI، تحت شماره دسترسی CBS11162 ثبت گردید. نتایج کروماتوگرافی لایه نازک و طیف‌سنجی مادون قرمز تبدیل فوریه تأیید کرد که بیوسورفکتانت استخراج شده سوفورولیپیدی با فعالیت سطحی قابل توجهی بود. سوفورولیپید خالص شده کشش سطحی آب را از mN/m 72 تا mN/m 1/29 کاهش داد. بیشترین شاخص امولسیون کنندگی، E24%، بیوسورفکتانت استخراج شده 53% به دست آمد و 71/63 تا 09/58 درصد از فعالیت اولیه خود را در دمای 80 تا 120 درجه سانتیگراد حفظ کرد. این بیوسورفکتانت همچنین به ترتیب 67/82 و 41/89 درصد از فعالیت اولیه خود را درpH 5/10، و 12، 66/59 درصد از فعالیت اولیه خود را در در شرایط شوری 10 درصد حفظ کرد.نتیجه‌‌گیری: این تحقیق اولین گزارش تولید سوفورولیپید توسط مخمر ردوترولا موسیلاژینوزا است. با توجه به پایداری حرارتی و تحمل pH و شوری بالا، سوفورولیپید تولید شده توسط ردوترولا موسیلاژینوزا می‌‌تواند به عنوان یک عامل امولسیون‌کننده مؤثر اکیداً برای کاربرد در برنامه‌‌های استخراج افزایش یافته میکروبی نفت و همچنین صنایع غذایی توصیه شود.
المصادر:
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Akbari E, Beheshti‐Maal K, Rasekh B, Emami‐Karvani Z & Omidi M. Isolation and Identification of Current Biosurfactant‐Producing Microbacterium maritypicum ABR5 as a Candidate for Oily Sludge Recovery. J Surfact Deterg. 2020; 23: 137-44.
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Mukherjee S, Das P & Sen R. Towards commercial production of microbial surfactants. Trends Biotechnol. 2006; 24: 509-15.
Astuti DI, Purwasena IA, Putri RE, Amaniyah M & Sugai Y. Screening and characterization of biosurfactant produced by Pseudoxanthomonas G3 and its applicability for enhanced oil recovery. J Petrol Explor Prod Technol. 2019; 9: 2279-89.
Alvarez VM, Jurelevicius D, Marques JM, de Souza PM, de Araújo LV, Barros TG, de Souza RO, Freire DM & Seldin L. Bacillus amyloliquefaciens TSBSO 3.8, a biosurfactant-producing strain with biotechnological potential for microbial enhanced oil recovery. Colloids Surf B. 2015; 136: 14-21.
Amani H, Sarrafzadeh MH, Haghighi M & Mehrnia MR. Comparative study of biosurfactant producing bacteria in MEOR applications. J Pet Sci Eng. 2010; 75: 209-14.
He C, Dong W, Li J, Li Y, Huang C & Ma Y. Characterization of rhamnolipid biosurfactants produced by recombinant Pseudomonas aeruginosa strain DAB with removal of crude oil. Biotechnol Lett. 2017; 39: 1381-8.
Sabati H & Motamedi H. Ecofriendly demulsification of water in oil emulsions by an efficient biodemulsifier producing bacterium isolated from oil contaminated environment. Biotechnol Lett. 2018; 40: 1037-48.
Lima TM, Procópio LC, Brandão FD, Carvalho AM, Tótola MR & Borges AC. Simultaneous phenanthrene and cadmium removal from contaminated soil by a ligand/biosurfactant solution. Biodegradation. 2011; 22: 1007-15.
Camargo FP, Araujo ACV, Moraes EM de & et al. A comparison between cactophilic yeast communities isolated from Cereus hildmannianus and Praecereus euchlorus necrotic Fungal Biol. 2016;120: 1175–83.
Konishi M, Maruoka N, Furuta Y, Morita T, Fukuoka T, Imura T & Kitamoto D. Biosurfactant-producing yeasts widely inhabit various vegetables and fruits. Biosci Biotechnol Biochem. 2014; 78: 516-23.
Mnif I & Ghribi D. Review lipopeptides biosurfactants: mean classes and new insights for industrial, biomedical, and environmental applications. Pep Sci. 2015; 104: 129-47.
Mousavi F, Beheshti-Maal K & Massah AR. Production of sophorolipid from an identified current yeast, Lachancea thermotolerans BBMCZ7FA20, isolated from honey bee. Curr Microbiol. 2015; 71: 303-10.
Mohawesh O, Janssen M, Maaitah O & et al. Assessment the effect of homogenized soil on soil hydraulic properties and soil water transport. Eurasian Soil Sci. 2017; 50: 1077–85.
Mousavi F, Beheshti-Maal K & Massah AR. Isolation of yeasts from bee bread of honey bees, Apis mellifera and evaluation of its ability to produce sophorolipid biosurfactant. Iranian J Med Microbiol. 2014; 8: 44–53.
Salehizadeh H & Mohammadizad S. Microbial enhanced oil recovery using biosurfactant produced by Alcaligenes faecalis. Iranian J Biotechnol. 2009; 7: 216-23.
Sen S, Borah SN, Bora A & Deka S. Production, characterization, and antifungal activity of a biosurfactant produced by Rhodotorula babjevae Microb Cell Fact. 2017; 16: 95.
Harju S, Fedosyuk H & Peterson KR. Rapid isolation of yeast genomic DNA: Bust n'Grab. BMC Biotechnol. 2004; 4: 8.
Akbari E, Beheshti-Maal K & Nayeri H. A novel halo-alkalo-tolerant bacterium, Marinobacter alkaliphilus ABN-IAUF-1, isolated from Persian Gulf suitable for alkaline lipase production. Int J Environ Sci Technol. 2018; 15: 1767-76.
Chen J, Song X, Zhang H & Qu Y. Production, structure elucidation and anticancer properties of sophorolipid from Wickerhamiella domercqiae. Enz Microb Technol. 2006; 39: 501-6.
Kurtzman CP, Price NP, Ray KJ & Kuo TM. Production of sophorolipid biosurfactants by multiple species of the Starmerella (Candida) bombicola yeast clade. FEMS Microbiol Lett. 2010; 311: 140-6.
Thaniyavarn J, Chianguthai T, Sangvanich P, Roongsawang N, Washio K, Morikawa M & Thaniyavarn S. Production of sophorolipid biosurfactant by Pichia anomala. Biosci Biotechnol Biochem. 2008; 72: 2061-8.
Fontes GC, Fonseca Amaral PF, Nele M & Zarur Coelho MA. Factorial design to optimize biosurfactant production by Yarrowia lipolytica. J Biomed Biotechnol. 2010; 23: 1-8.
Luna JM, Rufino RD, Sarubbo LA, Rodrigues LR, Teixeira JA & de Campos-Takaki GM. Evaluation antimicrobial and antiadhesive properties of the biosurfactant Lunasan produced by Candida sphaerica UCP 0995. Curr Microbiol. 2011; 62: 1527-34.
Almeida DG, Soares da Silva RD, Luna JM, Rufino RD, Santos VA & Sarubbo LA. Response surface methodology for optimizing the production of biosurfactant by Candida tropicalis on industrial waste substrates. Front Microbiol. 2017; 8: 157.
Camargo FP, Menezes AJ, Tonello PS, Dos Santos AC & Duarte IC. Characterization of biosurfactant from yeast using residual soybean oil under acidic conditions and their use in metal removal processes. FEMS Microbiol Lett. 2018; 365(10): fny098.
Song J, Rezaee S, Guo W, Hernandez B, Puerto M, Vargas FM, Hirasaki GJ & Biswal SL. Evaluating physicochemical properties of crude oil as indicators of low-salinity–induced wettability alteration in carbonate minerals. Sci Rep. 2020; 10:1-6.
Mahmoud M, Elkatatny S & Abdelgawad KZ. Using high- and low-salinity seawater injection to maintain the oil reservoir pressure without damage. J Petrol Explor Prod Technol. 2017; 7: 589-96.
_||_
Das MD. Application of biosurfactant produced by an adaptive strain of tropicalis MTCC230 in microbial enhanced oil recovery (MEOR) and removal of motor oil from contaminated sand and water. J Pet Sci Eng. 2018; 170: 40-8.
Thavasi R, Jayalakshmi S & Banat IM. Application of biosurfactant produced from peanut oil cake by Lactobacillus delbrueckii in biodegradation of crude oil. Biores Technol. 2011; 102: 3366-72.
Akbari E, Beheshti‐Maal K, Rasekh B, Emami‐Karvani Z & Omidi M. Isolation and Identification of Current Biosurfactant‐Producing Microbacterium maritypicum ABR5 as a Candidate for Oily Sludge Recovery. J Surfact Deterg. 2020; 23: 137-44.
Geetha SJ, Banat IM & Joshi SJ. Biosurfactants: Production and potential applications in microbial enhanced oil recovery (MEOR). Biocatal Agric Biotechnol. 2018; 14: 23-32.
Mukherjee S, Das P & Sen R. Towards commercial production of microbial surfactants. Trends Biotechnol. 2006; 24: 509-15.
Astuti DI, Purwasena IA, Putri RE, Amaniyah M & Sugai Y. Screening and characterization of biosurfactant produced by Pseudoxanthomonas G3 and its applicability for enhanced oil recovery. J Petrol Explor Prod Technol. 2019; 9: 2279-89.
Alvarez VM, Jurelevicius D, Marques JM, de Souza PM, de Araújo LV, Barros TG, de Souza RO, Freire DM & Seldin L. Bacillus amyloliquefaciens TSBSO 3.8, a biosurfactant-producing strain with biotechnological potential for microbial enhanced oil recovery. Colloids Surf B. 2015; 136: 14-21.
Amani H, Sarrafzadeh MH, Haghighi M & Mehrnia MR. Comparative study of biosurfactant producing bacteria in MEOR applications. J Pet Sci Eng. 2010; 75: 209-14.
He C, Dong W, Li J, Li Y, Huang C & Ma Y. Characterization of rhamnolipid biosurfactants produced by recombinant Pseudomonas aeruginosa strain DAB with removal of crude oil. Biotechnol Lett. 2017; 39: 1381-8.
Sabati H & Motamedi H. Ecofriendly demulsification of water in oil emulsions by an efficient biodemulsifier producing bacterium isolated from oil contaminated environment. Biotechnol Lett. 2018; 40: 1037-48.
Lima TM, Procópio LC, Brandão FD, Carvalho AM, Tótola MR & Borges AC. Simultaneous phenanthrene and cadmium removal from contaminated soil by a ligand/biosurfactant solution. Biodegradation. 2011; 22: 1007-15.
Camargo FP, Araujo ACV, Moraes EM de & et al. A comparison between cactophilic yeast communities isolated from Cereus hildmannianus and Praecereus euchlorus necrotic Fungal Biol. 2016;120: 1175–83.
Konishi M, Maruoka N, Furuta Y, Morita T, Fukuoka T, Imura T & Kitamoto D. Biosurfactant-producing yeasts widely inhabit various vegetables and fruits. Biosci Biotechnol Biochem. 2014; 78: 516-23.
Mnif I & Ghribi D. Review lipopeptides biosurfactants: mean classes and new insights for industrial, biomedical, and environmental applications. Pep Sci. 2015; 104: 129-47.
Mousavi F, Beheshti-Maal K & Massah AR. Production of sophorolipid from an identified current yeast, Lachancea thermotolerans BBMCZ7FA20, isolated from honey bee. Curr Microbiol. 2015; 71: 303-10.
Mohawesh O, Janssen M, Maaitah O & et al. Assessment the effect of homogenized soil on soil hydraulic properties and soil water transport. Eurasian Soil Sci. 2017; 50: 1077–85.
Mousavi F, Beheshti-Maal K & Massah AR. Isolation of yeasts from bee bread of honey bees, Apis mellifera and evaluation of its ability to produce sophorolipid biosurfactant. Iranian J Med Microbiol. 2014; 8: 44–53.
Salehizadeh H & Mohammadizad S. Microbial enhanced oil recovery using biosurfactant produced by Alcaligenes faecalis. Iranian J Biotechnol. 2009; 7: 216-23.
Sen S, Borah SN, Bora A & Deka S. Production, characterization, and antifungal activity of a biosurfactant produced by Rhodotorula babjevae Microb Cell Fact. 2017; 16: 95.
Harju S, Fedosyuk H & Peterson KR. Rapid isolation of yeast genomic DNA: Bust n'Grab. BMC Biotechnol. 2004; 4: 8.
Akbari E, Beheshti-Maal K & Nayeri H. A novel halo-alkalo-tolerant bacterium, Marinobacter alkaliphilus ABN-IAUF-1, isolated from Persian Gulf suitable for alkaline lipase production. Int J Environ Sci Technol. 2018; 15: 1767-76.
Chen J, Song X, Zhang H & Qu Y. Production, structure elucidation and anticancer properties of sophorolipid from Wickerhamiella domercqiae. Enz Microb Technol. 2006; 39: 501-6.
Kurtzman CP, Price NP, Ray KJ & Kuo TM. Production of sophorolipid biosurfactants by multiple species of the Starmerella (Candida) bombicola yeast clade. FEMS Microbiol Lett. 2010; 311: 140-6.
Thaniyavarn J, Chianguthai T, Sangvanich P, Roongsawang N, Washio K, Morikawa M & Thaniyavarn S. Production of sophorolipid biosurfactant by Pichia anomala. Biosci Biotechnol Biochem. 2008; 72: 2061-8.
Fontes GC, Fonseca Amaral PF, Nele M & Zarur Coelho MA. Factorial design to optimize biosurfactant production by Yarrowia lipolytica. J Biomed Biotechnol. 2010; 23: 1-8.
Luna JM, Rufino RD, Sarubbo LA, Rodrigues LR, Teixeira JA & de Campos-Takaki GM. Evaluation antimicrobial and antiadhesive properties of the biosurfactant Lunasan produced by Candida sphaerica UCP 0995. Curr Microbiol. 2011; 62: 1527-34.
Almeida DG, Soares da Silva RD, Luna JM, Rufino RD, Santos VA & Sarubbo LA. Response surface methodology for optimizing the production of biosurfactant by Candida tropicalis on industrial waste substrates. Front Microbiol. 2017; 8: 157.
Camargo FP, Menezes AJ, Tonello PS, Dos Santos AC & Duarte IC. Characterization of biosurfactant from yeast using residual soybean oil under acidic conditions and their use in metal removal processes. FEMS Microbiol Lett. 2018; 365(10): fny098.
Song J, Rezaee S, Guo W, Hernandez B, Puerto M, Vargas FM, Hirasaki GJ & Biswal SL. Evaluating physicochemical properties of crude oil as indicators of low-salinity–induced wettability alteration in carbonate minerals. Sci Rep. 2020; 10:1-6.
Mahmoud M, Elkatatny S & Abdelgawad KZ. Using high- and low-salinity seawater injection to maintain the oil reservoir pressure without damage. J Petrol Explor Prod Technol. 2017; 7: 589-96.
