Application of Magnetic Polymer Particles Modified with β–Cyclodextrin for Adsorption of Bovine Serum Albumin
الموضوعات :
Mahshid Nikpour Nezhati
1
(Department of Chemistry, Islamic Azad University Central Tehran branch, Tehran, Iran)
Habibollah Baharvand
2
(Department of Polymer Science, Iran Polymer & PetrochemicalInstitute,Tehran, Iran)
Zahra Ghorbani
3
(Department of Chemistry, Islamic Azad University Central Tehran branch, Tehran, Iran)
الکلمات المفتاحية:
ملخص المقالة :
Magnetic polymer particles which were modified by vinyl groups and subjected topolymerization by vinyl β-Cyclodextrin derivative, has been used as adsorbent for sorption andrelease of bovine serum albumin and the equilibrium and kinetics of the adsorption process werestudied. The absorbability and releasing of this protein through the new polymer has beenmeasured by ultraviolet-visible spectroscopy. Effects of various parameters such as pH, contacttime and capacity for adsorption of bovine serum albumin have been studied in vitro and theoptimum magnitudes were 4, 37.94 mg/g and 5 minutes respectively. The experimental resultsshow that the adsorption of Bovine serum albumin on magnetic particles was affected greatly bythe pH andthe adsorption equilibrium isotherm was fitted well by the Freundlich model. Bovineserum albumin was released from magnetic particles in simulated intestinal fluids. It wasreleased up to about 7 h at high speed and then it released slowly up to 30 h.
