Biochemical profiling and anticancer properties of brown seaweed Dictyota dichotoma (Hudson.) J.V.Lamouroux
الموضوعات :Velankanni Arputhaswamy 1 , Vasanthkumar Rajkumar 2 , Nancy Mary Thomas 3 , Vinoth Sathasivam 4 , Arun Muthukrishnan 5 , Gurusaravanan Packiaraj 6
1 - Plant Biotechnology Laboratory, Department of Botany, Bharathiar University, Coimbatore-641 046, Tamil Nadu, India
2 - Plant Biotechnology Laboratory, Department of Botany, Bharathiar University, Coimbatore-641 046, Tamil Nadu, India
3 - Plant Biotechnology Laboratory, Department of Botany, Bharathiar University, Coimbatore-641 046, Tamil Nadu, India
4 - Department of Biotechnology, Sona College of Arts and Science, Salem-636 005, Tamil Nadu, India
5 - Department of Biotechnology, Bharathiar University, Coimbatore-641 046, Tamil Nadu, India
6 - Plant Biotechnology Laboratory, Department of Botany, Bharathiar University, Coimbatore-641 046, Tamil Nadu, India
الکلمات المفتاحية: Anticancer, Antioxidant, Biochemical profiling, Dictyota dichotoma (Hudson.) J.V.Lamouroux, MCF-7 cancer cell,
ملخص المقالة :
In the present study, we have extracted the secondary metabolites from Dictyota dichotoma and evaluated their anticancer properties against Michigan Cancer Foundation-7 (MCF-7) breast cancer cell lines. Different solvent extracts were used to isolate the bioactive molecules form D. dichotoma. Among them, methanol extract showed the highest total phenol content (TPC: 306.31 ± 1.7 GAE mg/g extract), total tannin content (TTC: 291.90 ± 1.2 RE mg/g extract) and total flavonoid content (TFC: 92.89 ± 0.6 GAE mg/g extract). The antioxidant ABTS assay of methanol extract inhibited 74.51 ± 0.10% of free radicals and phosphomolybdenum assay showed 62.36 ± 0.04 mg/g AAE/g extract. Furthermore, bioactive compounds were characterised by gas chromatography-mass spectroscopy (GC-MS), highly performance liquid chromatography (HPLC) and Fourier transmission infrared spectroscopy (FT-IR). The GC-MS results revealed 29 bioactive compounds that are highly potential biological activities. The extracted compound was quantified and confirmed by HPLC and FT-IR. The D. dichotoma methanol extract effectively induced the apoptosis in MCF-7 breast cancer cells and recorded IC50 value of 44.35 ± 8.62 µg/mL.
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