Preliminary phytochemical screening for antioxidant activity and content of phenols and flavonoids of 18 species of plants native to western Ecuador
الموضوعات :
Shirley Moncayo
1
(Laboratory of Biotechnology, Faculty of Natural Sciences, University of Guayaquil, Av. Juan Tanca Marengo, 090601, Guayaquil, Ecuador)
Xavier Cornejo
2
(Herbarium GUAY, Department of Botany, University of Guayaquil, Av. Juan Tanca Marengo, 090601, Guayaquil, Ecuador)
Jhon Castillo
3
(Laboratory of Biotechnology, Faculty of Natural Sciences, University of Guayaquil, Av. Juan Tanca Marengo, 090601, Guayaquil, Ecuador)
Viviana Valdez
4
(Laboratory of Biotechnology, Faculty of Natural Sciences, University of Guayaquil, Av. Juan Tanca Marengo, 090601, Guayaquil, Ecuador)
الکلمات المفتاحية: Ecuadorian plants, Flavonoids, phenols, DPPH•,
ملخص المقالة :
The phytochemical screening, total phenolics and flavonoids content and antioxidant activity of plants native to western Ecuador were investigated to provide the basic information for further studies towards the discovery of new compounds. The species studied were Adenostemma platyphyllum Cass, Castilla elastica subsp . gummifera (Miq.) C.C. Berg., Cochlospermum vitifolium (Willd.) Spreng., Ectozoma pavonii Miers, Erythrochiton giganteus Kaastra & A.H. Gentry, Erythroxylum patens Ruiz ex O.E.Schulz, Ficus brevibracteata W.C. Burger, Ficus tonduzii Standl., Grias ecuadorica Cornejo & S.A. Mori, Handroanthus billbergii subsp . ampla (Bureau K. Schum.) S.O. Grose, Morisonia americana L., Operculina codonantha (Benth.) Hallier f., Passiflora macrophylla Spruce ex Mast., Podandrogyne jamesonii (Briq.) Cochrane, Pradosia montana T.D. Penn., Tecoma castaneifolia (D.Don) Melch, Urera baccifera (L.) Gaudich. ex Wedd., and Xanthosoma sagittifolium (L.) Schott.The highest percentage of inhibition of DPPH• was found in E. patens (92.41%). Most of the extracts evaluated exhibited antioxidant activity.
Abdel-Hameed, E.-S.S., 2009. Total phenolic contents and free radical scavenging activity of certain Egyptian Ficus species leaf samples. Food Chem. 114(4), 1271-1277.
Abdel-Lateif, K.S., Eldeab, H.A., Maghrabi, I., 2016. The plant natural products: their antioxidants, free radical scavengers, DNA protection and antimicrobial activities. J. Bioprocess. Biotech. 6(9), 1-7.
Abubakar, I.B., Lim, K.-H., Loh, H.-S., 2015. Alkaloid extracts of Ficus species and palm oil-derived tocotrienols synergistically inhibit proliferation of human cancer cells. Nat. Prod. Res. 29(22), 2137-2140.
Aguilar-Guadarrama, A., Rios, M.Y., 2018. Flavonoids, sterols and lignans from Cochlospermum vitifolium and their relationship with its liver activity. Molecules 23(8), 1-8.
Aguirre, Z., Kvist, L. P., Sánchez, O., 2006. Bosques secos en Ecuador y su diversidad, in: M. Moraes R., B.Ø., L. P. Kvist, F. Borchsenius & H. Balslev (Ed.), Botánica Económica de los Andes Centrales. Universidad Mayor de San Andrés, La Paz, pp. 162-187.
Ayoub, L., Hassan, F., Hamid, S., Abdelhamid, Z., Souad, A., 2019. Phytochemical screening, antioxidant activity and inhibitory potential of Ficus carica and Olea europaea leaves. Bioinformation 15(3), 226-232.
Babbar, N., Oberoi, H.S., Uppal, D.S., Patil, R.T., 2011. Total phenolic content and antioxidant capacity of extracts obtained from six important fruit residues. Food Res. Int. 44(1), 391-396.
Baj, T., Kukula-Koch, W., Świątek, Ł., Zielińska-Pisklak, M., Adamska-Szewczyk, A., Szymczyk, D., Rajtar, B.,Polz-Dacewicz, M., Skalicka-Woźniak, K., 2017. Chemical profile, antioxidant activity and cytotoxic effect of extract from leaves of Erythrochiton brasiliensis Nees & Mart. from different regions of Europe. Open Chem. J. 15(1), 380-388.
Benvenutti, R.C., Dalla Vecchia, C.A., Locateli, G., Serpa, P.Z., Lutinski, J.A., Junior, S.A.R., Corralo, V., Gutiérrez, M.V., Vilegas, W., Somensi, L.B., 2020. Gastroprotective activity of hydroalcoholic extract of the leaves of Urera baccifera in rodents. J. Ethnopharmacol. 250, 1-45.
Berg, C.C., Corner, E.J.H., Jarrett, F.M., 2006. Moraceae genera other than Ficus. Flora Malesiana 17(1),1-152.
Borkataky, M., Kakoty, B., Saikia, L., 2013. Influence of total phenolic content and total flavonoid content on the DPPH radical scavenging activity of Eclipta alba (L.) Hassk. Int. J. Pharm. Pharm. Sci. 5(1), 224-327.
Bors, W., Heller, W., Michel, M., Saran, M., 1990. Flavonoids as antioxidants: determination of radical-scavenging efficiencies. Methods Enzymol. 186, 343-355.
Brand-Williams, W., Cuvelier, M.-E., Berset, C., 1995. Use of a free radical method to evaluate antioxidant activity. Food Sci. Technol. 28(1), 25-30.
Brauchli, P., Schindler, O., Reichstein, T., 1961. Die Cardenolide vonCastilla elastica CERV. Glykoside und aglykone, 222. Mitteilung. Helv. Chim. Acta 44(4), 904-919.
Choudhury, S., Datta, S., Talukdar, A., Choudhury, M., 2011. Phytochemistry of the Family Bignoniaceae - A review. Assam Univ J. Sci. Technol: Biol. Environ. Sci. 7(1), 145-150.
Cochrane, T.S., 1997. Podandrogyne hispidula, P. jamesonii, and P. mathewsii (Capparidaceae), three valid species from Ecuador and Peru. Novon 7(4), 353-354.
Cornejo, X., Mori, S.A., 2012. Grias angustipetala and G. ecuadorica, two new species of Lecythidaceae from western Ecuador. Brittonia 64(3), 318-324.
Costa, E.V.S., Brígido, H.P.C., Coelho-Ferreira, M.R., Brandão, G.C., Dolabela, M.F., 2017. Antileishmanial activity of Handroanthus serratifolius (Vahl) S. Grose (Bignoniaceae). Evid. Based Complement. Alternat. Med. 8074275, 1-6.
Costa, M.T., da Silva Goulart, A., de Jesus Soares, J., Salgueiro, A.C.F., da Rosa, H.S., Denardin, E.L.G., Paim, C.S., Puntel, R.L., Folmer, V., 2020. Antioxidant and toxicological potential of the Golden trumpet hydroalcoholic stem bark extract. Res. Soc. Dev. 9(4), 1-22.
De la Torre, L., Navarrete, H., Muriel, P., Macía, M., Balslev, H., 2008. Enciclopedia de las Plantas Útiles del Ecuador, 1 ed. Herbario QCA de la Escuela de Ciencias Biológicas de la Pontificia Universidad Católica del Ecuador & Herbario AAU del Departamento de Ciencias Biológicas de la Universidad de Aarhus, Quito, Ecuador.
De la Torre, L., Cerón, C.E., Balslev, H., Borchsenius, F., 2012. A biodiversity informatics approach to ethnobotany: Meta-analysis of plant use patterns in Ecuador. Ecol. Soc. 17(1), 1-17.
Derakhshan, Z., Ferrante, M., Tadi, M., Ansari, F., Heydari, A., Hosseini, M.S., Conti, G.O., Sadrabad, E.K., 2018. Antioxidant activity and total phenolic content of ethanolic extract of pomegranate peels, juice and seeds Food Chem. Toxicol. 114, 108-111.
Díaz, L., 2009. Interacciones moleculares entre plantas y microorganismos: saponinas como defensas químicas de las plantas y su tolerancia a los microorganismos. Una revisión. RET Rev. Estud. Transdisc. 1(2), 32-55.
Dos Santos, C., Antunes, P.S., Santos, F.L.P.d., Rocha, A.d.O.B., Pita, J.C.L.R., Xavier, A.L., Macêdo, C.L., Jacob, K.C., de Oliveira, N.A., de Medeiros, A.A.N., 2016. Assessment of Pradosia huberi effects on the reproductive system of male rats. Exp. Biol. Med. 241(5), 519-526.
Doyle, B., Svobodny, G., Batallas, R., Fernández,D., 2012. Medical ethnobotany of the amazonian Kichwa community of San José De Payamino, Ecuador: preliminary results from an undergraduate-level field course. In: International Symposium on Medicinal Plants and Natural Products 2012. Acta Hortic. 18,103-108.
Dring, J., Kite, G., Nash, R., Reynolds, R., 1995. Chemicals in aroids: a survey, including new results for polyhydroxy alkaloids and alkylresorcinols. Bot. J. Linn. Soc. 117(1), 1-12.
Eliaser, E., Hui Ho, J., Rukayadi, Y., Lian Ee, G.C., Abdull Razis, A.F., 2018. Phytochemical constituents and biological activities of Melicope lunu-ankenda. Molecules 23(10), 1-22.
Farag, R.S., Abdel-Latif, M.S., Abd El Baky, H.H., Tawfeek, L.S., 2020. Phytochemical screening and antioxidant activity of some medicinal plants’ crude juices. Biotechnol. Rep. (Amst) 28 (e00536), 1-7.
Fauzan, A., Praseptiangga, D., Hartanto, R., Pujiasmanto, B., 2018. Characterization of the chemical composition of Adenostemma lavenia (L.) Kuntze and Adenostemma platyphyllum Cass. Paper presented at the OP Conference Series: Earth and Environmental Science, 102 (012029), 1-9.
García, E., Medina, M., 2006. Composición química, metabolitos secundarios, valor nutritivo y aceptabilidad relativa de diez árboles forrajeros. Zootecnia Trop. 24(3), 233-250.
Gimeno Creus, E., 2004. Compuestos fenólicos. Un análisis de sus beneficios para la salud. Offarm 23(6), 80-84.
Gindri, A., De Souza, L., Cruz, R., Boligon, A., Machado, M., Athayde, M., 2014. Genotoxic evaluation, secondary metabolites and antioxidant capacity of leaves and roots of Urera baccifera Gaudich (Urticaceae). Nat. Prod. Res. 28(23), 2214-2216.
González, J., Ayala, A., Gutiérrez, E., 2006. Determinación de fenoles totales y taninos condensados en especies arbóreas con potencial forrajero de la Región de Tierra Caliente Michoacán, México. Livest. Res. Rural. Dev. 18(11), 152. http://www.lrrd.org/lrrd18/11/guti18152.htm
Gonzalez, K., Gonzalez, J.A., González, J., Prieto, S., 2005. Género Erythroxylum: análisis de la información científica. Acta farm. Bonaer. 24(2), 284-290.
Gonzalez, E., Gómez, M., 2012. Terpene compounds in nature: a review of their potential antioxidant activity. Curr. Med. Chem. 19(31), 5319-5341.
Goupy, P., Hugues, M., Boivin, P., Amiot, M.J., 1999. Antioxidant composition and activity of barley (Hordeum vulgare) and malt extracts and of isolated phenolic compounds. J. Sci. Food Agric. 79(12), 1625-1634.
Guamán-Ortiz, L.M., Romero-Benavides, J.C., Suarez, A.I., Torres-Aguilar, S., Castillo-Veintimilla, P., Samaniego-Romero, J., Ortiz-Diaz, K., Bailon-Moscoso, N., 2020. Cytotoxic property of Grias neuberthii extract on human colon cancer cells: A crucial role of autophagy. Evid. Based Complement. Alternat. Med. 2020(565306), 1-11.
Harborne, A., 1998. Phytochemical Methods. A Guide to Modern Techniques of Plant Analysis, 3 ed. Springer Science & Business Media, London, UK.
Harling, G., Andersson, L., 1986-1998. Flora of Ecuador vols. 25-60. Department of Systematic Botany, University of Göteborg & Section for Botany, Riksmuseeum, Stockholm.
Hopia, A., Heinonen, M., 1999. Antioxidant activity of flavonol aglycones and their glycosides in methyl linoleate. J. Am. Oil Chem. Soc. 76(1), 139-144.
Ibarra, E., Pacheco, M., García, R., San Miguel, R., Ramírez , G., Soto, R.M., 2011. Actividad antioxidante de alcaloides de Erythrina americana Miller. Rev. Fitotec. Mex. 34(4), 241-246.
Jerzykiewicz, J., 2007. Alkaloids of Solanaceae (nightshade plants). Postepy Biochem. 53(3), 280-286.
Ku, Y.G., Kim, H.C., Bae, J.H., Kang, B.S., Nemirovski, A., Barasch, D., Gorinstein, S., 2019. Antioxidant capacities and polyphenols in autumn-growing cultivar of Chinese cabbage (Brassica rapa L. ssp. pekinensis cv. Bulam Plus). Eur. Food Res. Technol. 245(9), 1871-1879.
Kumar, S., Pandey, A., 2013. Chemistry and biological activities of flavonoids: an overview. Sci. World J. 2013(162750), 1-16.
Lobo, V., Patil, A., Phatak, A., Chandra, N., 2010. Free radicals, antioxidants and functional foods: Impact on human health. Pharmacogn. Rev. 4(8), 118-126.
Lorz, L.R., Yoo, B.C., Kim, M.-Y., Cho, J.Y., 2019. Anti-wrinkling and anti-melanogenic effect of Pradosia mutisii methanol extract. Int. J. Mol. Sci. 20(5), 1-18.
Ludwiczuk, A., Skalicka-Woźniak, K., Georgiev, M., 2017. Terpenoids, in: Simone, B., Rupika, D. (Eds.), Pharmacognosy. Fundamentals, Applications and Strategies. Elsevier, pp. 233-266.
Markert, A., Steffan, N., Ploss, K., Hellwig, S., Steiner, U., Drewke, C., Li, S.-M., Boland, W., Leistner, E., 2008. Biosynthesis and accumulation of ergoline alkaloids in a mutualistic association between Ipomoea asarifolia (Convolvulaceae) and a clavicipitalean fungus. Plant Physiol. 147(1), 296-305.
Mascarenhas, M.E., Mandrekar, C. R., Marathe, P. B., & Morais, L. J., 2017. Phytochemical screening of selected species from Convolvulaceae. Int. J. Curr. Pharm. Res. 9(6), 94-97.
Murillo, E., Lombo, O., Tique, M., Méndez, J.J., 2007. Potencial antioxidante de Bauhinia kalbreyeri Harms (FABACEAE). Inf. Tecnol. 18(6), 65-74.
Mustafa, R., Hamid, A.A., Mohamed, S., Bakar, F.A., 2010. Total phenolic compounds, flavonoids, and radical scavenging activity of 21 selected tropical plants. J. Food Sci. 75(1), C28-C35.
Mwamatope, B., Tembo, D., Chikowe, I., Kampira, E., Nyirenda, C., 2020. Total phenolic contents and antioxidant activity of Senna singueana, Melia azedarach, Moringa oleifera and Lannea discolor herbal plants. Sci. African 9(e00481), 1-7.
Niaz, K., Khan, F., 2020. Analysis of polyphenolics, in: Nabavi, S. F., Saeedi, M., Nabavi, S. M., Sanches A. (Eds.), Recent Advances in Natural Products Analysis. Elsevier, pp. 39-197.
Nishanthini, A., Mohan, V.R., 2012. Antioxidant activites of Xanthosoma sagittifolium Schott using various in vitro assay models. Asian Pac. J. Trop. Biomed 2(3), S1701-S1706.
Ortíz, L., Chaves, G., 2017. Composición fitoquímica del extracto de raíz de Ichthyothere terminalis de dos regiones geográficas de Colombia. Rev. Colomb. Quim. 46(3),11-16.
Pagare, S., Bhatia, M., Tripathi, N., Pagare, S., Bansal, Y.K., 2015. Secondary metabolites of plants and their role: Overview. Curr. Trends Biotechnol. Pharm. 9 (3), 293-304.
Pękal, A., Pyrzynska, K., 2014. Evaluation of aluminium complexation reaction for flavonoid content assay. Food Anal. Methods 7(9), 1776-1782.
Pichersky, E., Gang, D., 2000. Genetics and biochemistry of secondary metabolites in plants: an evolutionary perspective. Trends Plant Sci. 5(10), 439-445.
Quiñones, M., Miguel, M., Aleixandre, A., 2012. Los polifenoles, compuestos de origen natural con efectos saludables sobre el sistema cardiovascular. Nutr. Hosp. 27(1), 76-89.
Ramakrishna, A., Ravishankar, G.A., 2011. Influence of abiotic stress signals on secondary metabolites in plants. Plant Signal Behav. 6(11), 1720-31.
Ramawat, K.G., Dass, S., Mathur, M., 2009. The Chemical Diversity of Bioactive Molecules and Therapeutic Potential of Medicinal Plants, in: Ramawat, K. (Ed.), Herbal Drugs: Ethnomedicine to Modern Medicine. Springer, Berlin, Heidelberg, (Chapter 2), pp. 7-32.
Ramírez-Ortíz, M.E., Rodríguez-Carmona, O.Y., Hernández-Rodríguez, O.S., Chel-Guerrero, L.A., Aguilar-Méndez, M.Á., 2016. Estudio de la actividad hipoglucemiante y antioxidante de tronadora, wereque y raíz de nopal, in: Ortiz, M.E.R. (Ed.), Alimentos Funcionales de Hoy. OmniaScience Monographs, Barcelona, España, pp. 143-180.
Ranjitham, P., Jamuna, S., Padmavathi, K., Paulsamy, S., 2014. In vitro antioxidant properties of the traditional medicinal plant species, Ehretia microphylla Lam. and Erythroxylon monogynum Roxb. Res. J. Pharm. Biol. Chem. Sci. 5(1), 247-252.
Restrepo, D.A., Saenz, E., Jara-Muñoz, O.A., Calixto-Botía, I.F., Rodríguez-Suárez, S., Zuleta, P., Chavez, B.G., Sanchez, J.A., D’Auria, J.C., 2019. Erythroxylum in focus: An interdisciplinary review of an overlooked genus. Molecules 24(20), 3788.
Rivas, K.E., Muñoz, D.L., Balcázar Morales, N., 2015. Actividad antioxidante, contenido fenólico total y citotoxicidad de extractos polares obtenidos de plantas antidiabéticas colombianas. Rev. Cuba. de Plantas Medicinales 20(3), 277-289.
Roginsky, V., Lissi, E.A., 2005. Review of methods to determine chain-breaking antioxidant activity in food. Food Chem. 92(2), 235-254.
Rondón, M., Moncayo, S., Cornejo, X., Plaza, C., 2018a. Fenoles totales, contenido de flavonoides y actividad antioxidante de los extractos etanólicos de plantas ecuatorianas. Rev. Fac. Farm. 60(2), 3-13.
Rondón, M., Moncayo, S., Cornejo, X., Santos, J., Villalta D., Siguencia, R., Duche, J., 2018b. Preliminary phytochemical screening, total phenolic content and antibacterial activity of thirteen native species from Guayas province Ecuador. J. King Saud Univ. Sci. 30(4), 500-505.
Roy, A., McDonald, P., Timmermann, B.N., Gupta, M., Chaguturu, R., 2019. Bioactivity Profiling of plant biodiversity of panama by high throughput screening. Nat. Prod. Commun. 14(1), 71-74.
Salgado, J., Palacio, A., Valero, N., 2020. Actividad antioxidante e influencia del periodo climático sobre el contenido de polifenoles totales en Merremia aegyptia. Biotecnol. sector agropecuario agroind. 18(2), 82-93.
Sengul, M., Yildiz, H., Gungor, N., Cetin, B., Eser, Z., Ercisli, S., 2009. Total phenolic content, antioxidant and antimicrobial activities of some medicinal plants. Pak. J. Pharm. Sci. 22(1), 102-106.
Shaikh, J.R., Patil, M., 2020. Qualitative tests for preliminary phytochemical screening: An overview. Int. J. Chem. Stud. 8(2), 603-608.
Singleton, V.L., Orthofer, R., Lamuela-Raventós, R.M., 1999. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin Ciocalteu reagent. Methods Enzymol. 299, 152-178.
Sirisha, N., Sreenivasulu, M., Sangeeta, K., Chetty, C.M., 2010. Antioxidant properties of Ficus species-a review. Int. J. Pharm.Tech. Res. 2(4), 2174-2182.
Souza, J.S.d.S.d., 2018. Caracterização nutricional, fitoquímica e biológica da Taioba (Xanthosoma sagittifolium (L.) Shott). Master, Universidade Federal do Paraná, Curitiba, Brasil.
Subedi, L., Timalsena, S., Duwadi, P., Thapa, R., Paudel, A., Parajuli, K., 2014. Antioxidant activity and phenol and flavonoid contents of eight medicinal plants from Western Nepal. J. Tradit. Chin. Med. 34(5), 584-590.
Tripathi, I.P., Mishra, C., 2015. Phytochemical screening of some medicinal plants of Chitrakoot region. Indian J. Appl. Res. 5(12), 56-60.
Vidhya, R., Fleming, A.T., 2015. Cytotoxicity analysis of crude leaf extracts from Tecoma castanifolia (D. Don) Melch on Brine Shrimp and MCF-7 Cell Line. Int. J. Sci. Res. 4(12), 1405-1408.
Vuolo, M.M., Lima, V.S., Junior, M.R.M., 2019. Phenolic Compounds: Structure, Classification, and Antioxidant Power, in: Campos, M.R.S. (Ed.), Bioactive Compounds. Elsevier, pp. 33-50.
Wagner, K.-H., Elmadfa, I., 2003. Biological relevance of terpenoids. Overview focusing on mono-di and tetraterpenes. Ann. Nutr. Metab 47(3-4), 95-106.
Witharana, E., Wijetunga, W., Sathanandan, P., Wijesinghe, S., Wickramaratne, L., 2014. Cardiac toxicity with nodal bradycardia due to Panama rubber (Castilla elastica: Moraceae) poisoning. Ceylon Med. J. 59(3), 97-98.
Zhishen, J., Mengcheng, T., Jianming, W., 1999. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem. 64(4), 555-559.
