Anti-inflammatory potential of aqueous extract and ethyl acetate fractions of Munronia pinnata (Wall) Theob. and the isolated compound, Senecrassidiol

الموضوعات :

Swarna Hapuarachchi ¹ , Thusharie Suresh ² , Shiroma Hadunnetthi ³ , Wathuge Thamara Priyanthie Sandun Senarath ⁴ , Chandani Ranasinghe ⁵

1 - Unit of Pharmacology and Pharmaceutics, Department of Ayurveda, Institute of Indigenous Medicine, University of Colombo, Sri Lanka
2 - Department of Biochemistry, Faculty of Medical Sciences, University of Sri Jayewardenepura, Sri Lanka
3 - Institute of Biochemistry, Molecular Biology, and Biotechnology, University of Colombo, Sri Lanka
4 - Department of Botany, Faculty of Applied Sciences, University of Sri Jayewardenepura, Sri Lanka
5 - Department of Chemistry, Faculty of Natural Sciences, The Open University of Sri Lanka

تاريخ الإرسال : 30 الأربعاء , ذو القعدة, 1443 تاريخ التأكيد : 22 الأحد , صفر, 1444 تاريخ الإصدار : 05 الخميس , صفر, 1444

الکلمات المفتاحية: <i>Munronia pinnata</i>, Wistar Rats, Anti-inflammatory, Membrane stabilization, Folk medicine, Senecrassidiol,

ملخص المقالة :

This study was designed to evaluate in vivo and in vitro anti-inflammatory potential of the different extracts of Munronia pinnata and compare it with the isolated compound, Senecrassidiol by using the carrageenan-induced paw edema animal model in Wistar rats. Munronia pinnata (Wall) Theob (Meliaceae) has been traditionally practiced for hundreds of years by traditional physicians as a home remedy for the treatment of inflammatory conditions in folk medicine in Sri Lanka. The tested ethyl acetate fractions of methanol extract and its isolated compound senecrassidiol exhibited significant anti-inflammatory activity in the tested models. It demonstrated the scientific rationale for the use of an anti-inflammatory agent in folk medicine. The MPaq showed a significant (p < 0.5) inhibition of infiltration of rat peritoneal cells, inhibition of NO production by rat peritoneal cells, and inhibition of membrane stabilization as well as the anti-histamine activity which are the probable anti-inflammatory mechanisms.

المصادر:

Adnan, M.S.A., Al-Amin, M.M., Uddin, M.M.N., Shohel, M, Bhattacharjee, R, Hannan J.M.A. Das., B.K, 2014. Analgesic, anti-inflammatory, and antipyretic effects of Ixora coccinea. J. Basic Clin. Physiol. Pharmacol. 25(4), 423-428.
Arawwawala, M., Thabrew, I., Arambewela, L., Handunnetti, S., 2010. Anti-inflammatory activity of Trichosanthes cucumerina Linn. in rats. J. Ethnopharmacol.131, 538-543. 
Arciniegas, A., Maldonado, J.I., Gonzalez, K, Perez-Castorena, L.A., Villasenor, J.L., Vivar, A.R.D., 2013. Sesquiterpenes and other natural products from Roldana reticulata. J. Mex. Chem. Soc. 57 (1),16-18.
Arulmozhi, S., Mazumder, P.M., Sathiyanarayan, L., Thakurdesai, P.A., 2012. Anti-inflammatory and anti-ulcerogenic activities of fractions from Alistonia scholaris. Pharmacologia 3(5),132-137. 
Chakraborty, M., Bhattacharya, S., Bose, M., Sasmal, S.K., Gupta, M., Mishra, R., 2017. Anti-inflammatory activity of cyclo-oxygenase2 inhibitory anionic protein fraction from Lamellidens marginalis (Lamarck). Indian J. Exp. Biol. 55, 88-97. 
Chao, W., Kuo, Y.H., Lin, B.F., 2010. Anti-inflammatory activity of new compounds from Andrographis paniculata by NF-κB transactivation inhibition. J. Agric. Food Chem. 58, 2505-2512. 
Chioma, A.A., Onyechi, O., Lawrance, Ezeanyika, U.S., 2012. Membrane stabilization as a mechanism of the anti-inflammatory activity of methanol extract of garden egg (Solanum aethiopicum). J. Pharma. Sci. 20(1), 1-7. 
Dassanayake, M.D., Fosberg, F.R., Clayton, W.D., 1995. A Revised Handbook to the Flora of Ceylon. Smithsonian Institute and the National Science foundation. Washington DC. Amerind Co. Ltd. New Delhi, ix, pp. 236-239.
Dharmadasa, R.M., Samarasinghe, K., Adhihetty, P., Hettiarachchi, P.L., 2013. Comparative pharmacognostic evaluation of Munronia Pinnata (Wall.) Theob. (Meliaceae) and its substitute Andrographis paniculata (Burm.f.) Wall. Ex Nees (Acanthaceae). World J. Agric. Res.1 (5), 77-81. 
Hapuarachchi, S.D., Suresh, T.S., Senarath, W.T.P.S.K., 2011a. A preliminary study of the oral hypoglycaemic activity of ethanolic and water extracts of Munronia pinnata in healthy Wistar Rats. SLJIM 1(1),1-4.
Hapuarachchi, S.D., Suresh, T.S., Senarath, W.T.P.S.K., Dhanayake, J.M., 2011b. Hypoglycaemic effect of a decoction (aqueous extract) of Munronia pinnata in the healthy Wistar Rats. Planta Med. 77, 561.
Hapuarachchi, S.D., Suresh, T.S., Senarath, W.T.P.S.K., Hadunnetthi, S., 2012. Anti-inflammatory activity of the decoction of M. pinnata. Proceedings of the National Ayurvedic Research Conference. Sri Lanka, p. 34. 
Hapuarachchi, S., Suresh, T., Senarath, S., 2020. Safety assessment of the water extract of Munronia pinnata in Wistar rats. Int. J. Herb. Med. 8(6), 15-18.
Handunnetti, S.M., Kumara, R.R., Deraniyagala, S.A., Ratnasooriya, W.D., 2009. Anti-inflammatory activity of Ixora coccinea methanol extract. Pharmacol. Res.1, 80-90. 
Huang, Q., Zhao, M., Zhao, K., 2014. Alteration of vascular permeability in burn injury. Med. Expr.1(2), 62-76.
Jayasekara, K.G., Soysa, S.S.S.B.D.P., Suresh, T.S., Goonasekara, C.L., Gunasekera, K.M., 2022. A preliminary study on the anti-dengue viral activity of Munronia pinnata. Curr. Sci. 25(1), 65-74.
Jayasinghe, D.M., Buddhadasa, H.K., Rajapaksa, D.H., Jayathilaka, K.G.P., 1976. Ayurveda Pharmacopeia. Department of Ayurveda, Sri Lanka, 1(1),132-172.
Jayaweera, D.M.A., Senarathne, L.K., 1982. Medicinal Plants Used in Ceylon, The National Science Foundation Council, Sri Lanka.2(4), 341.
Katz, M., 2006. Study Design and Statistical analysis. A Practical Guide for Clinicians, Cambridge University Press, London.
Kumar, V., Fausto, N., Abbas, A., 2009. Acute and chronic inflammation. Robbins and Cortran Pathologic Basis of Disease. (Ed 8). Saunders Elsevier, pp. 47-87.
Kumari, K.D.K.P., Weerakoon, T.C.S., Handunnetti, S.M., Samarasinghe, K., Suresh, T.S., 2014. Anti-inflammatory activity of dried flower extracts of Aegle marmelos in Wistar rats. J. Ethnopharmacol. 151(3), 1202-1208.
Li, X.-L., He, Q.-X., Zhang, F.-L., Zhao, Y.-L., Liu, K.-C., Jiang, S-P., 2012. Chemical constituents from Munronia sinica and their bioactivities. Nat. Prod. Biopropect. 2, 76-80. 
Lin, B-D., Chen, H.-D., Liu, J., Zhang, S., Wu, Y., Dong, L., Yue, J.-M., 2010. Mulavanins A-E: Limonoids from Munronia delavayi. Phytochemistry 71,1596-1601.
Maccioni, M., Cabezeas, L. E., Rivero, V.E., 2003. Effect of prostatein, the major protein produced by the rat ventral prostate, on phagocytic cell functions. Am. J. Reprod. Immunol. 50(3), 473-480.
Mehta, M., Dureja, H., Garg, M., 2015. Development and optimization of boswellic acid-loaded proniosomalgel. Drug Deliv. 23(8), 3072-3081. 
Mohan, V.R., Balamurugan, K., Sakthidevi, G., 2012. Anti-inflammatory activity of whole plant of Polycarpaea corymbosa (L) LAM (Caryophyllaceae). Int. J. Pharm. Sci. 3(4), 3336-3341. 
Mounnissamy, V.M., Kavimani, S., Balu, V., Drlin, Q.S., 2007. Evaluation of anti-inflammatory and membrane stabilizing properties of ethanol extract of Cansjera reheedii Gmelin (Opiliaceae). Iranian J. Pharmacol. Therapeut. 6(2), 235-23. 
Munasinghe, M.L.A.M.S., 2002. Phytochemical screening of Munronia pumila (wall.) Theob. Ayurveda Sameekshava 1(11), 164-171.
Napagoda, M., Gerstmeier, J., Koeberle, A., Wesely, S., Popella, S., Lorenz, S., Scheubert, K., Bocker, S., Svatose, A., Werz, O., 2014. Munronia pinnata (Wall.) Theob. unveiling phytochemistry and dual inhibition of 5- lipoxygenase and microsomal prostaglandin E2 synthase (mPGES)-1. J. Ethnopharmacol. 151(2), 882-890.
Qi, S.-H., Wu, D.-G., Chen, L., Ma, Y.-B., Luo, X.-D., 2003. Insect antifeedants from Munronia henryi: Structure of Munronoamide. J. Agric. Food Chem. 51, 6949-6952.
Reddy, D.B., Reddy, T.C., Jyotsna, G., Sharan, S., Priya, N., Lakshmipathi, V., Reddanna P., 2009. Chebulagic acid, a COX-LOX dual inhibitor isolated from the fruits of Terminalia chebula Retz. induces apoptosis in COLO-205 cell line. J. Ethnopharmacol. 30, 506-512. 
Reddy, V.P., Urooj, A., 2013. Antioxidant properties and stability of Aegle marmelos leave extract. J. Food Sci. Technol. 5,135-140. 
Sekhar, S., Karmakar, R., Siddapura, R.N., Prakash, S.H., 2011. Potential anti-inflammatory bioactives from medicinal plants of Western Ghats, India. Pharmacog. Commun. 2, 2-12. 
Sugita, R., Kuwabara, H., Kubota, K., Sugimoto, K., Kiho, T., Tengeiji, A., Kawakami, K., Shimada, K., 2016. Simultaneous inhibition of PGE2 and PGI2 signals is necessary to suppress hyperalgesia in rat inflammatory pain models. Mediators Inflamm. 39(2), 907-915. 
Trease, E., Evans, W.C., 2008. Pharmacognosy.15 Ed. London, Saunders Publisher.
Wallace, J.L., 2005. Nitric oxide as a regulator of inflammatory processes. Mem. do Inst. Oswaldo Cruz.100 (Suppl.1), 5-9. 
Winter, C.A., Risley, E.A., Nuss, G.W., 1962. Carrageenan-induced oedema in hind paw of rat, an assay for anti-inflammatory drugs. Proc. Soc. Exp. Biol. Med. 111,544-547. 
Zhao, J., Maitituersun, A., Li, C., Li, Q., Xu, F., Liu, T., 2018. Evaluation on analgesic and anti-inflammatory activities of total flavonoids from Juniperus sabina. Evid. Based Complement. Altern. Med. 2018, 7965306. doi: 10.1155/2018/7965306.