Blood coagulation effect of combined extract of Thymus vulgaris and Medicago sativa
الموضوعات :
Zahra Sadat Mashkani
1
(Department of Biology, Hakim Sabzevari University, Sabzevar, Iran)
Jafar Vatandoost
2
(Department of Biology, Hakim Sabzevari University, Sabzevar, Iran)
Toktam Hajjar
3
(Department of Biology, Hakim Sabzevari University, Sabzevar, Iran)
Behnam Mahdavi
4
(Department of Chemistry, Hakim Sabzevari University, Iran)
الکلمات المفتاحية: Thymus vulgaris, Medicago sativa, Herbal medicine, Hemophilia,
ملخص المقالة :
Thymus vulgaris and Mediacgo sativa, are used as a traditional remedy in the treatment of bleeding disorders. Considering their probabilistic coagulation compounds, in an animal study, forty male mice were randomly divided into 5 groups (n = 8) as well as negative and positive control. Coagulation indices include bleeding time (BT), clotting time (CT), and the number of platelets (PLT) were examined on the 13th day of treatment. A significant reduction in the BT and CT tests, as well as a significant increase in PLT in the treated groups was observed. It is concluded that although the T. vulgaris and M. sativa extracts have a coagulation effect through primary homeostasis and a common pathway of secondary hemostasis, combined extracts are more effective than individual extracts. Moreover, phenolic and flavonoid compounds are the most affecting compounds that affect platelet number and aggregation.
Abasalizadeh, M., Nehzati, P., Allahbakhshian, M., Hamidpour, M., Khadem Mabudi, A.A., Tabatabaie, M.R., 2017. The detection of Scavenger Receptor-B1 expression and its role on the function of platelets in patients with atherosclerotic disease. Sci J Iran Blood Transfus Organ. 14(2), 92-100.
Abdallah, L., Surakji, I., Qawasme, T., Ayyash, D., Shhadeh, R., Omar, G., Barakat, A., 2022. In vitro activity of some medicinal plants on blood coagulation. Turk J Pharm Sci. 19(3), 330-335.
Aghili, Z.S., Zarkesh-Esfahani, S.H., 2016. Study of effect fetal bovine serum on morphology, viability and recombinant human growth hormone production in compared to serum free media in CHO cells. New cell. Mol. Biotech. 6(24), 65-72.
Akbarzadeh, S., Ostovar, A., Angali, N., Abbasifard, A., Chashmpoosh, M., 2019. Effects of hydroalcoholic extract of Thymus vulgaris on serum levels lipoprotein lipase and angiopoietin-like protein 4 in hyperlipidemic rats. Maz.UMS. 28(170), 22-32.
Barber, A., Green, D., Galluzzo, T., Ts'ao, C.H., 1985. The bleeding time as a preoperative screening test. Am. J. Med. 78(5), 761-764.
Bassampoor, S.S., 2002. Nursing Cares in tissue plastiminogen activator therapy. Hayat 8(1), 53-59.
Beaulieu, L.M., Lin, E., Mick, E., Koupenova, M., Weinberg, E.O., Kramer, C.D., Genco, C.A., Tanriverdi, K., Larson, M.G., Benjamin, E.J., 2014. Interleukin 1 receptor 1 and interleukin 1β regulate megakaryocyte maturation, platelet activation, and transcript profile during inflammation in mice and humans. Arterioscl. Throm. Vas. 34(3), 552-564.
Bécherel, P.-A., Le Goff, L., Ktorza, S., Chosidow, O., Francès, C., Issaly, F., Mencia-Huerta, J.-M., Debré, P., Mossalayi, M.D., Arock, M., 1996. CD23-mediated nitric oxide synthase pathway induction in human keratinocytes is inhibited by retinoic acid derivatives. J Invest Dermatol. 106(6), 1182-1186.
Bilen, S., Altunoglu, Y.C., Ulu, F., Biswas, G., 2016. Innate immune and growth promoting responses to caper (Capparis spinosa) extract in rainbow trout (Oncorhynchus mykiss). Fish Shellfish Immunol. 57, 206-212.
Brahimi, M., Osmani, S., Arabi, A., Enta-Soltan, B., Taghezout, Z., Elkahili, B.S., Bekadja, M.A., 2009. The estimation of platelet count from a blood smear on the basis of the red cell: platelet ratio. Turk J Haematol 26(1):21-24.
Chen, C.-Y., Milbury, P.E., Lapsley, K., Blumberg, J.B., 2005. Flavonoids from almond skins are bioavailable and act synergistically with vitamins C and E to enhance hamster and human LDL resistance to oxidation. J. Nutr. 135(6), 1366-1373.
Crescente, M., Jessen, G., Momi, S., Höltje, H.-D., Gresele, P., Cerletti, C., De Gaetano, G., 2009. Interactions of gallic acid, resveratrol, quercetin and aspirin at the platelet cyclooxygenase-1 level. Functional and modelling studies. J Thromb Haemost. 102(08), 336-346.
De Caterina, R., Lanza, M., Manca, G., Strata, G.B., Maffei, S., Salvatore, L., 1994. Bleeding time and bleeding: an analysis of the relationship of the bleeding time test with parameters of surgical bleeding. 3363-3370.
Deng, L., Qi, Y., Liu, Z., Xi, Y., Xue, W., 2019. Effect of tannic acid on blood components and functions. Colloids Surf B Biointerfaces. 184, 110505.
El-Newary, S.A., Shaffie, N.M., Omer, E., 2017. The protection of Thymus vulgaris leaves alcoholic extract against hepatotoxicity of alcohol in rats. Asian Pac. J. Trop. Med. 10(4), 361-371.
Elyasi, H., Rahimi, H., Kiani, A.A., 2017. A review of medicinal herbs capable of preventing blood coagulation and platelet aggregation. Herb. Med. J. 2(4),158-165.
Emeka, P.M., 2021. In-vitro studies of Bryophyllum pinnatum crude extract on blood coagulation indices: an investigation on its traditional medicine use. Pharmacogn. Mag. 13(4), 268-273.
Enayati, N., Ghafarzadegan, R., Hajiaghaee, R., Vazirian, M., 2017. comparison of differnt extraction methods for the extraction of bioactive components of Senna alexandrina. J Med Plants. 16(64), 160-169.
Fauzi, A., Lamma, S., Ruslin, M., 2018. Total tannin levels analysis of brown algae (Sargassum sp. and Padina sp.) to prevent blood loss in surgery. J. dentomaxillofacial sci. 3(1), 37-40.
Fazeli-nasab, B., Moshtaghi, N., Forouzandeh, M., 2019. Effect of solvent extraction on phenol, flavonoids and antioxidant activity of some iranian native herbs. J. Ilam Uni. Med. Sci. 27(3), 14-26.
Franchini, M., 2013. The modern treatment of haemophilia: a narrative review. Blood transfus 11(2), 178–182.
Hamidpour, M., 2014. The effect of low molecular weight lipoproteins on the formation of vascular thrombus. Blood Res. 11(3), 221-229.
Hashemi Tayer, A., Amirizadeh, N., Almasi-Hashiani, A., Kamravan, M., Mohammadi, M., 2013. A new fibrin sealant: hemostatics evaluation in the animal model. Sci J Iran Blood Transfus Organ. 10(3), 289-296.
Hosseini, F., Mortazavi, S.A., Bazaz, B., Koocheki, A., Bolorian, S., 2010. Study on the antimicrobial effect of Thymus vulgaris extract on the log (CFU/g) Salmonella enteritidise PT4 in mayonnaise. Int. J. Eng. Sci. Technol. 6(2), 84-90.
Hosseinzadeh, S., Jafarikukhdan, A., Hosseini, A., Armand, R., 2015. The application of medicinal plants in traditional and modern medicine: a review of Thymus vulgaris. Int J Clin Exp Med. 6(9), 635-642.
Huang, L., Lin, C., Li, A., Wei, B., Teng, J., Li, L., 2010. Pro-coagulant activity of phenolic acids isolated from Blumea riparia. Nat. Prod. Commun. 5(8), 1263-6
Javed, H., Erum, S., Tabassum, S., Ameen, F., 2013. An overview on medicinal importance of Thymus vulgaris. J. Asian Sci. Res. 3(10), 974-982.
Kaeidi, A., Rahmani, M., Hassanshahi, J., 2020. The protective effect of carvacrol and thymol as main polyphenolic compounds of thyme on some biologic systems in disease condition: A narrative review. J. Rafsanjan Univ. Med. Sci. Health Serv. 19(1), 81-96.
Karimi, E., Oskoueian, E., Oskoueian, A., Omidvar, V., Hendra, R., Nazeran, H., 2013. Insight into the functional and medicinal properties of Medicago sativa (Alfalfa) leaves extract. J. Med. Plant Res. 7(7), 290-297.
Klotoé, J., Dougnon, T., Sacramento, T., Dandjesso, C., Edorh, A., Koudokpon, H., Fanou, V., Fah, L., Ategbo, J., Loko, F., 2012. Hemostatic potential of the sap of Musa sapientum L.(Musaceae). J. Appl. Pharm. Sci. 2(7), 65-69.
Klotoe , J.R., Ategbo , J.M., Dougnon, V., 2017. Hemostatic effect of Jatropha multifida L.(Euphorbiaceae) in rats having coagulation disorders. J Appl Biol Biotechnol. 5(5), 26-29.
Kuete, V., 2017. Thymus vulgaris. Medicinal spices and vegetables from Africa, 1st Edition Therapeutic potential against metabolic, inflammatory, infectious and systemic diseases. 599-609.
Lee, Y.Y., Lee, S., Jin, J.L., Yun-Choi, H.S., 2003. Platelet anti-aggregatory effects of coumarins from the roots of Angelica genuflexa and A. gigas. Arch. Pharmacal Res. 26, 723-726.
Lethagen, S., 2003. Desmopressin in mild hemophilia A: indications, limitations, efficacy, and safety. Semin thromb hemost 29(1), 101-106.
Lynch, T.A., Miya, T., Carr, C.J., 1958. An investigation of the blood coagulating principles from Ceanothus Americanus. J. Am. Pharm. Assoc. 47(11), 816-819.
Mancini, E., Senatore, F., Del Monte, D., De Martino, L., Grulova, D., Scognamiglio, M., Snoussi, M., De Feo, V., 2015. Studies on chemical composition, antimicrobial and antioxidant activities of five Thymus vulgaris L. essential oils. Molecules 20(7), 12016-12028.
Mirza, M., Baher, Z., 2003. Chemical composition of essential oil from Thymus vulgaris hybrid. Essent. Oil Res. 15(6), 404-405.
Norris, L.A., 2003. Blood coagulation. Best Pract Res Clin Obstet Gynaecol. 17(3), 369-383.
Ogston, D., 1982. Inhibition of the plasminogen activator urokinase by alpha-tocopherol. Acta Haematol. 67(2), 114-118.
Palta, S., Saroa, R., Palta, A., 2014. Overview of the coagulation system. Indian J. Anaesth. 58(5), 515-523.
Pawlaczyk, I., Czerchawski, L., Kańska, J., Bijak, J., Capek, P., Pliszczak-Król, A., Gancarz, R., 2010. An acidic glycoconjugate from Lythrum salicaria L. with controversial effects on haemostasis. J Ethnopharmacol. 131(1), 63-69.
Peana, A.T., Marzocco, S., Popolo, A., Pinto, A., 2006. (−)-Linalool inhibits in vitro NO formation: Probable involvement in the antinociceptive activity of this monoterpene compound. Life Sci. 78(7), 719-723.
Pietrzak, K., Grela, E.R., 2015. Influence of alfalfa protein concentrate dietary supplementation on blood parameters of growing-finishing pigs. J Vet Res. 59(3), 393-399.
Poston, H.A., 1964. Effect of dietary vitamin K and sulfaguanidine on blood coagulation time, microhematocrit, and growth of immature brook trout. Prog Fish C. 26(2), 59-64.
Raaof, A., Al-Naqqash, Z.A.-E., Jawad, A.-L.M., Muhsan, S.M., 2013a. Evaluation of the activity of crude alkaloids extracts of Zingiber officinale Roscoe, Thymus vulgaris L. and Acacia arabica L. as coagulant agent in lab mice. Biomed biotechnol. 1(2), 11-16.
Raaof, A., Al-Naqqash, Z.A.-E., Jawad, A.-L.M., Muhsan, S.M., 2013b. Evaluation of the activity of crude alkaloids extracts of Zingiber officinale Roscoe, Thymus vulgaris L. and Acacia arabica L. as coagulant agent in lab mice. J Biomed Biotechnol .1, 11-16.
Radtke, O.A., Foo, L.Y., Lu, Y., Kiderlen, A.F., Kolodziej, H., 2003. Evaluation of sage phenolics for their antileishmanial activity and modulatory effects on interleukin-6, interferon and tumour necrosis factor-α-release in RAW 264.7 Cells. Z. Naturforsch. 58(5-6), 395-400.
Rehman, A.A., Riaz, A., Asghar, M.A., Raza, M.L., Ahmed, S., Khan, K., 2019. In vivo assessment of anticoagulant and antiplatelet effects of Syzygium cumini leaves extract in rabbits. BMC Complement Altern Med. 19(1), 1-8.
Singh, J.M., Singh, M.D., 1975. Alkaloids of tobacco and blood coagulation: effect of nicotine on thrombin and fibrinogen. Clin. Toxicol. 8(1), 43-52.
Stuhlmeier, K., Theyer, G., Baumgartner, G., Zlabinger, G., 1991. Synergistic effect of coumarin (1, 2 benzopyrone) and endotoxin in the induction of human interleukin-1. Clin. Exp. Immunol. 84(2), 317-323.
Tajika, K., Ikebuchi, K., Inokuchi, K., Hasegawa, S., Dan, K., Sekiguchi, S., Nakahata, T., Asano, S., 1998. IL‐6 and SCF exert different effects on megakaryocyte maturation. Br. J. Haematol. 100(1), 105-111.
Triratana, T., Suwannuraks, R., Naengchomnong, W., 1991. Effect of Eupatorium odoratum on blood coagulation. J Med Assoc Thai. 74(5), 283-287.
Uwai, K., Osanai, Y., Imaizumi, T., Kanno, S.-i., Takeshita, M., Ishikawa, M., 2008. Inhibitory effect of the alkyl side chain of caffeic acid analogues on lipopolysaccharide-induced nitric oxide production in RAW264. 7 macrophages. Bioorg. Med. Chem. 16(16), 7795-7803.
Vatandoost, J., Mashkani, Z.S., Hajjar, T., Mahdavi, B., 2022. The coagulant effect of the Medicago sativa L. hydroalcoholic extract: an In vivo study on mice. Herb Med. J. 7(2), 1-8.
Windyga, J., Solano Trujillo, M.H., Hafeman, A.E., 2014. BAX326 (RIXUBIS): a novel recombinant factor IX for the control and prevention of bleeding episodes in adults and children with hemophilia B. Ther Adv Hematol. 5(5), 168-180.
Xuan, T.D., Tsuzuki, E., Terao, H., Matsuo, M., Khanh, T.D., 2003. Correlation between growth inhibitory exhibition and suspected allelochemicals (phenolic compounds) in the extract of alfalfa (Medicago sativa L.). Plant Prod. Sci. 6(3), 165-171.
Yang, M., Qian, X.-H., Zhao, D.-H., Fu, S.-Z., 2010. Effects of Astragalus polysaccharide on the erythroid lineage and microarray analysis in K562 cells. J. Ethnopharmacol. 127(2), 242-250.
Zagórska-Dziok, M., Ziemlewska, A., Nizioł-Łukaszewska, Z., Bujak, T., 2020. Antioxidant activity and cytotoxicity of Medicago sativa L. seeds and herb extract on skin cells. BioResearch 9(1), 229-242.
