Phytochemical study and anti-inflammatory activity of the roots of Mangifera indica L. in lipopolysaccharide (LPS)-stimulated peritoneal macrophages
الموضوعات :
Yolande Nangue
1
(Department of Chemistry, Faculty of Sciences, P.O. Box 24157, University of Douala, Cameroon)
Eulogio José Llorent-Martínez
2
(Department of Physical and Analytical Chemistry, University of Jaén, Campus Las Lagunillas S/N, E-23071 Jaén, Spain)
Maria Luisa Fernández-de Córdova
3
(Department of Physical and Analytical Chemistry, University of Jaén, Campus Las Lagunillas S/N, E-23071 Jaén, Spain)
Douglas Alphonse M. Ngangoum
4
(Department of Animal Biology, Faculty of Sciences, P.O. Box 67, University of Dschang, Cameroon)
Télesphore Benoit Nguelefack
5
(Department of Animal Biology, Faculty of Sciences, P.O. Box 67, University of Dschang, Cameroon)
Anatole Guy Blaise Azebaze
6
(Department of Chemistry, Faculty of Sciences, P.O. Box 24157, University of Douala, Cameroon)
Alain Bertrand Dongmo
7
(Department of Animal Biology and Physiology, Faculty of Sciences, P.O. Box 24157, University of Douala, Cameroon)
الکلمات المفتاحية: Cytotoxicity, nitric oxide, Anti-inflammatory, phenolic compounds, <i>Mangifera indica</i>,
ملخص المقالة :
This study deals with the identification of secondary metabolites of methanol extract of the roots of Mangifera indica L and the assessment of plant in vitro anti-inflammatory activity. High-performance liquid chromatography with electrospray ionization mass spectrometric detection (HPLC-ESI-MSn) using the negative ion mode was performed to establish the chromatographic fingerprint and identify various chemical components of the plant extract. The anti-inflammatory effect of the MeOH extract (3, 30 and 300 μg/mL) was assessed through cell viability and nitric oxide (NO) production on non-stimulated and LPS-stimulated peritoneal macrophages. Phytochemical analysis indicated the presence of a number of phenolic compounds where galloyl derivatives, mangiferin and its derivatives were the major constituents. The methanol extract exhibited significant concentration-dependent inhibitory effect on NO production, both on stimulated and non-stimulated macrophages. The concentration 300 μg/mL showed significant cell toxicity. The methanol extract of Mangifera indica is rich in phenolic compounds and possesses potent in vitro anti-inflammatory activity, but its higher concentrations are cytotoxic.
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