Antimicrobial activity and toxicity of Eucalyptus globulus Labill. essential oil against vaginal microorganisms
الموضوعات :
Mirjana Bogavac
1
(University of Novi Sad, Faculty of Medicine, Clinical Centre of Vojvodina, Department of Obstetrics and Gynecology, Hajduk Veljkova 3, 21000 Novi Sad, Serbia)
Kristina Tešanović
2
(University of Belgrade, Faculty of Biology, Studentskitrg 16, 11000 Belgrade, Serbia)
Jovana Marić
3
(University of Novi Sad, Faculty of Sciences, Department of Biology and Ecology, Trg D. Obradovića 2, 21000 Novi Sad, Serbia)
Mirjana Jovanović
4
(Clinical Center of Serbia, Clinic for Gynecology and Obstetrics, Koste Todorovića 26, 11000 Belgrade, Serbia)
Maja Karaman
5
(University of Novi Sad, Faculty of Sciences, Department of Biology and Ecology, Trg D. Obradovića 2, 21000 Novi Sad, Serbia)
الکلمات المفتاحية: Toxicity, Essential oils, Antimicrobial Activity, <i>Eucalyptus</i>,
ملخص المقالة :
The antimicrobial potential of commercial essential oil (EO) of Eucalyptus globulus L. was evaluated against six bacterial vaginal isolates (E. coli 1 and E. coli 2, S. aureus 1 and S. aureus 2, P. aeruginosa and P. mirabilis) and two isolates of Candida strains (C. albicans 1 and C. albicans 2). The antimicrobial activity was assessed through double-dilution micro-plate assay to determine the minimum inhibitory (MIC) and minimum bactericidal/fungicidal (МВС/ MFC) concentration. In addition, brine shrimp toxicity assay was performed in order to determine acute toxicity of the examined EO. The isolated strains of pathogens have shown strain specificity to the tested EO. Certain pathogens resistance was noticed toward the tested antibiotic, as well. E. coli isolates showed resistance to the tested antibiotics but did not show resistance against the Eucalyptus EO (E. coli 1 MIC/MBC 12.5/12.5 μL/mL; E. coli 2 MIC/ MBC 25/25 μL/mL). Moreover, the Eucalyptus EO showed effectiveness in application against S. aureus 2 (MIC/MBC 6.25/6.25 μL/mL), and C. albicans 1 strains (MIC/MFC 6.25/6.25 μL/mL). Furthermore, brine shrimp lethality bioassay revealed the Eucalyptus oil toxicity at an LC50 value of 2.66 mg/mL. The chemical composition of the separated essential oil was analyzed by gas chromatography combined with mass spectrometry (GC-MS) showing eucalyptol (59.63%), p-cymene (15.55%) and DL-limonene (14.90%) as dominant constituents. Although a number of toxicology trials are needed, these results provide scientific support to examination of Eucalyptus EO as an antimicrobial agent in alternative treatment of multiresistant human pathogens of vaginal origin.
Bachir, R. G., Benali, M., 2012. Antibacterial activity of the essential oils from the leaves of Eucalyptus globulus against Escherichia coli and Staphylococcus aureus. Asian Pac. J. Trop. Biomed. 2(9), 739-742.
Bogavac, M., Lakic, N., Simin, N., Nikolic, A., Sudji, J., Bozin, B., 2012. Bacterial vaginosis and biomarkers of oxidative stress in amniotic fluid. J. Matern.-Fetal Neonatal Med. 25(7), 1050-1054.
Bortz, J., Levinson, R. S., Kirschner, M., Cuca, R.C., 2013. U.S. Patent Application No. 13/557,527.
Burt, S., 2004. Essential oils: their antibacterial properties and potential applications in foods-a review. Int. J. Food Microbiol. 94(3), 223-253.
Carson, C. F., Hammer, K.A., 2011. Chemistry and Bioactivity of Essential Oils. Lipids and Essential Oils as Antimicrobial Agents, pp. 203-238.
Christoph, F., Kaulfers, P.M., Stahl-Biskup, E., 2000. A comparative study of the in vitro antimicrobial activity of tea tree oils sp. with special reference to the activity of β-triketones. Planta Med. 66(06), 556-560.
Clinical Laboratory Standards Institute, 2008. M02-A11-Performance Standards for Antimicrobial Disk Susceptibility Tests; Approved Standard, 11th Ed. Wayne PA, USA: Clinical and Laboratory Standard Institute. CLSI Document.; M07-A9-Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically; Approved Standard, 9th Ed. Wayne, PA, USA: Clinical and Laboratory Standard Institute. CLSI Document.
Damjanović-Vratnica, B., Ðakov, T., Šuković, D., Damjanović, J., 2011. Antimicrobial effect of essential oil isolated from Eucalyptus globulus Labill. from Montenegro. Czech J. Food Sci. 29(3), 277-284.
Frezza, C., Venditti, A., Serafini, I., Carassiti, A., Foddai, S., Bianco, A., Serafini, M., 2017. Phytochemical characteristics of Galeopsisladanum subsp. angustifolia(Ehrh. ex Hoffm.) Gaudin collected in Abruzzo region (Central Italy) with chemotaxonomic and ethnopharmacological implications. Trends Phytochem. Res. 1(2), 61-68.
Griffin, S. G., Wyllie, S. G., Markham, J. L., Leach, D. N., 1999. The role of structure and molecular properties of terpenoids in determining their antimicrobial activity. Flav. Frag. J. 14(5), 322-332.
Hinou, J.B., Harvala, C.E., Hinou, E.B., 1989. Antimicrobial activity screening of 32 common constituents of essential oils. Pharmazie 44(4), 302.
Knobloch, K., Pauli, A., Iberl, B., Weis, N., Weigand, H., 1988. Mode of action of essential oil components on whole cells of bacteria and fungi in plate tests. In Bioflavour 87, 287-299.
Meyer, B. N., Ferrigni, N. R., Putnam, J. E., Jacobsen, L. B., Nichols, D. J., McLaughlin, J. L., 1982. Brine shrimp: a convenient general bioassay for active plant constituents. Planta Med. 45(05), 31-34.
Mohammadhosseini, M., Sarker, S.D., Akbarzadeh, A., 2017. Chemical composition of the essential oils and extracts of Achillea species and their biological activities: A review. J. Ethnopharmacol. 199, 257-315.
Mohammadhosseini, M., Venditti, A., Sarker, S.D., Nahar, L., Akbarzadeh, A., 2019. The genus Ferula: Ethnobotany, phytochemistry and bioactivities – A review. Ind. Crop Prod. 129, 350-394.
Mordi, N., Ismail, S., Mansor, S.M., 2010. Brine shrimp lethality and acute oral toxicity studies on Swietenia mahagoni (Linn.) Jacq. seed methanolic extract. Pharmacognosy Res. 2(4), 215.
Mulyaningsih, S., Sporer, F., Zimmermann, S., Reichling, J., Wink, M., 2010. Synergistic properties of the terpenoids aromadendrene and 1,8-cineole from the essential oil of Eucalyptus globulus against antibiotic-susceptible and antibiotic-resistant pathogens. Phytomedicine 17(13), 1061-1066.
Naeini, A., Khosravi, A.R., Chitsaz, M., Shokri, H., Kamlnejad, M., 2009. Anti-Candida albicans activity of some Iranian plants used in traditional medicine. J. Mycol. Méd. 19(3), 168-172.
Parra, A.L., Yhebra, R.S., Sardiñas, I.G., Buela, L.I., 2001. Comparative study of the assay of Artemia salina L. and the estimate of the medium lethal dose (LD50 value) in mice, to determine oral acute toxicity of plant extracts. Phytomedicine 8(5), 395-400.
Raman, A., Weir, U., & Bloomfield, S. F., 1995. Antimicrobial effects of tea‐tree oil and its major components on Staphylococcus aureus, Staph. epidermidis and Propionibacterium acnes. Lett. Appl. Microbiol. 21(4), 242-245.
Pierce, A.M.,Hart, CA., 1992. Vulvovaginitis: causes and management. Arch. Dis. Child. 67(4), 509-512.
Sahgal, G., Ramanathan, S., Sreenivasan Sasidharan, M., Mordi, N., Ismail, S., Mansor, S.M., 2010. Brine shrimp lethalityand acute oral toxicity studies on Swietenia mahagoni (Linn.) Jacq. seed methanolic extract. Phcog. Res. 2(4), 215.
Salari, M. H., Amine, G., Shirazi, M. H., Hafezi, R., Mohammadypour, M., 2006. Antibacterial effects of Eucalyptus globulus leaf extract on pathogenic bacteria isolated from specimens of patients with respiratory tract disorders. Clin. Microbiol. Infect. 12(2), 194-196.
Sarker, S.D., Nahar, L., 2018a. Evidence-based phytotherapy: what, why and how? Trends Phytochem. Res. 2(3), 125-126.
Sarker, S.D., Nahar, L., 2018b. Phytochemicals and phyto-extracts in cosmetics. Trends Phytochem. Res. 2(4), 185-186.
Sherry, E., Boeck, H., Warnke, P.H., 2001. Topical application of a new formulation of Eucalyptus oil phytochemical clears methicillin-resistant Staphylococcus aureus infection. Am. J. Infect. Control. 29(5), 346.
Silva, J., Abebe, W., Sousa, S.M., Duarte, V.G., Machado, M.I.L., Matos, F.J.A., 2003. Analgesic and anti-inflammatory effects of essential oils of Eucalyptus. J. Ethnopharmacol. 89(2-3), 277-283.
Trivedi, N.A., Hotchandani, S.C., 2004. A study of the antimicrobial activity of oil of Eucalyptus. Indian J. Pharmacol. 36(2), 93.
Tzakou, O., Pitarokili, D., Chinou, I.B., Harvala, C., 2001. Composition and antimicrobial activity of the essential oil of Salvia ringens. Planta Med. 67(01), 81-83.
Vermani, K., Garg, S., 2002. Herbal medicines for sexually transmitted diseases and AIDS. J. Ethnopharmacol. 80(1), 49-66.
Wansi, J.D., Sewald, N., Nahar, L., Martin, C., Sarker, S.D., 2018. Bioactive essential oils from the Cameroonian rain forest: A review - Part I. Trends Phytochem. Res. 2(4), 187-234.
Wansi, J.D., Sewald, N., Nahar, L., Martin, C., Sarker, S.D., 2019. Bioactive essential oils from the Cameroonian rain forest: A review - Part II. Trends Phytochem. Res. 3(1), 3-52.
Warnke, P.H., Becker, S.T., Podschun, R., Sivananthan, S., Springer, I.N., Russo, P.A., Sherry, E., 2009. The battle against multi-resistant strains: renaissance of antimicrobial essential oils as a promising force to fight hospital-acquired infections.
J. Craniomaxillofac. Surg. 37(7), 392-397.
Noumi, E., Snoussi, M., Hajlaoui, H., Trabelsi, N., Ksouri, R., Valentin, E., Bakhrouf, A., 2011. Chemical composition, antioxidant and antifungal potential of Melaleuca alternifolia (tea tree) and Eucalyptus globulus essential oils against oral Candida species. J. Med. Plants Res. 5(17), 4147-4156.
Jarić, S., Popović, Z., Mačukanović-Jocić, M., Djurdjević, L., Mijatović, M., Karadžić, B., Pavlović, P. 2007. An ethnobotanical study on the usage of wild medicinal herbs from Kopaonik Mountain (Central Serbia). J. Ethnopharmacol. 111(1), 160-175.
Zlatković, B. K., Bogosavljević, S. S., Radivojević, A. R., Pavlović, M. A. 2014. Traditional use of the native medicinal plant resource of Mt. Rtanj (Eastern Serbia): Ethnobotanical evaluation and comparison. J. Ethnopharmacol. 151(1), 704-713.
Sartorelli, P., Marquioreto, A.D., Amaral‐Baroli, A., Lima, M.E.L., Moreno, P.R.H., 2007. Chemical composition and antimicrobial activity of the essential oils from two species of Eucalyptus. Phytother. Res. 21(3), 231-233.
