القاء چاقی، عصاره سیر و استویا به‌همراه فعالیت هوازی، بر بیان ژن سمافورین4D و گیرنده‌ی پلکسین-B2 در موش‌های نر صحرائی
الموضوعات :
ملیحه اردکانی زاده
1
(دانشگاه دامغان، گروه علوم ورزشی)
سید جواد ضیاءالحق
2
(گروه فیزیولوژی ورزشی واحد شاهرود، دانشگاه آزاد اسلامی ، شاهرود، ایران.)
الکلمات المفتاحية: چاقی, عصاره سیر, عصاره استویا, سمافورینD4, پلکسینB2.,
ملخص المقالة :
زمینه و هدف: ازدیرباز، میوه سیر و برگ استویا در کنار فعالیت بدنی هوازی، به‌عنوان یک ضد التهاب بیان شده‌اند، ازطرفی به‌تازگی سمافورین‌ها به‌عنوان پروتئین‌های اثرگذار بر التهاب سیستم عصبی مرکزی شناسائی شده‌اند. مواد و روش‌ها: تعداد 35 سر رت نژاد ویستار نر چاق شده (شاخص لی) بصورت تصادفی به 7 گروه (پنج تایی)؛ کنترل سالم، چاق، سیر، استویا، هوازی، سیر+هوازی، و استویا+هوازی تقسیم شدند. تمرینات هوازی شامل30 دقیقه/روز، پنج روز/هفته بود، و عصاره سیر و استویا با غلظت 250 میلی‌گرم/کیلوگرم به آب مصرفی روزانه اضافه شد. جهت مطالعات بیان ژن سمافورین 4Dو گیرنده آن بنام پلکسینB2 به روش real-time PCR بافت مغز به آزمایشگاه ارسال شد. از آزمون آنالیز واریانس یک‌طرفه و آزمون تعقیبی توکی جهت تعیین اختلاف بین گروه‌ها استفاده گردید (05/0p≤). نتایج: مقادیر بیان ژن سمافورینD4 گروه چاق نسبت به گروه کنترل سالم افزایش معنی‌دار داشت، و همه گروه‌ها غیر از گروه استویا+هوازی، کاهش معنی‌داری در میزان این ژن نشان دادند. مقادیر پلکسینB2 گروه استویا+هوازی از مقادیر گروه کنترل چاق نیز فراتر بود، و گروه سیر با/بدون فعالیت هوازی مقادیر کمتری نسبت به گروه کنترل سالم و چاق تجربه کردند (05/0>p). نتیجه گیری: به‌نظر می‌رسد که القاء چاقی موجب افزایش بیان ژن سمافورین4D و گیرنده پلکسینB2 می‌گردد، و عصاره سیر و ترکیب آن با فعالیت هوازی اثرات مفیدی بر کاهش التهاب سیستم عصبی مرکزی ایفا می‌کنند.
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