نقش سترورلیکس در حفاظت از تغییرات مورفولوژیکی و فراساختاری اپی‌تلیوم ژرمینال بافت بیضه به دنبال آسیب‌های ایجادشده توسط سیکلوفسفامید در موش سوری
الموضوعات :
داریوش محمدنژاد
1
(دانشیار مرکز تحقیقات کاربردی دارویی، دانشگاه علوم پزشکی تبریز، تبریز، ایران.)
جمال عیوضی ضیایی
2
(دانشیار مرکز تحقیقات هماتولوژی و انکولوژی، دانشگاه علوم پزشکی تبریز، تبریز، ایران.)
ایدا اعظمی
3
(کارشناس ارشد مرکز تحقیقات کاربردی دارویی، دانشگاه علوم پزشکی تبریز، تبریز، ایران.)
محمدرضا ولیلو
4
(استادیار گروه پاتوبیولوژی، دانشکده دامپزشکی، واحد تبریز، دانشگاه آزاد اسلامی، تبریز، ایران.)
حسین راستا
5
(دانشیارگروه فیزیک پزشکی، دانشکده پزشکی، دانشگاه علوم پزشکی تبریز، تبریز، ایران.)
علی عابدالهی
6
(دانشیار مرکز تحقیقات هماتولوژی و انکولوژی، دانشگاه علوم پزشکی تبریز، تبریز، ایران.)
تاريخ الإرسال : 25 الأحد , محرم, 1442
تاريخ التأكيد : 11 السبت , جمادى الأولى, 1442
تاريخ الإصدار : 05 الخميس , ربيع الأول, 1442
الکلمات المفتاحية:
بیضه,
سترورلیکس,
موش سوری,
سیکلوفسفامید,
ملخص المقالة :
داروهای مورد استفاده در درمان سرطان‌ها، اثرات مضر در ارگان‌های با تقسیمات سریع سلولی از جمله بافت بیضه دارند. آنتاگونیست های گنادوتروپین با مهار تقسیم در سلول‌های اسپرماتوژنیک باعث حفاظت سلول‌های مذکور از عوارض داروهای شیمی درمانی از جمله سیکلوفسفامید می‌گردند. هدف از انجام این مطالعه، بررسی توانایی سترورلیکس (آنتاگونیست گنادوتروپین)، در مهار اثرات سوء سیکلوفسفامید در بافت بیضه موش سوری بود. بدین منظور 30 سر موش سوری بالغ نر 8-6 هفته ای به 3 گروه کنترل (هیچ دارویی را دریافت نکردند)، آزمایش 1 (دریافت کنندهmg/kg ۵۰وزن بدن،سیکلوفسفامید به‌صورت داخل صفاقی) و آزمایش 2 (دریافت کننده mg/kg ۲۵/۰ وزن بدن،سترورلیکس به صورت زیرجلدی به همراه سیکلوفسفامید به‌میزانmg/kg ۵۰ وزن بدن) تقسیم شدند. 35 روز بعد از آخرین تزریق سترورلیکس، همه موش‌ها تحت بیهوشی کشته شده و بیضه آن ها برای مطالعات هیستومورفومتریک و فراساختاری برداشته شد. مطالعه لوله‌های سمینی‌فر با میکروسکوپ نوری نشان دهنده کاهش شدید سلول‌های اسپرماتوژنیک و ضخامت اپی‌تلیوم ژرمینال و کاهش معنی‌دار میانگین شاخص اسپرمیوژنز (spermiogenesis index; SI) و تعداد سلول‌های سرتولی در گروه آزمایش 1 بود (05/0p < /em><). مطالعه میکروسکوپ الکترونی نیز نشان دهنده آسیب به سلول‌های سرتولی و پیدایش فضاهای وسیع و متعددی در بین این سلول‌ها و سلول‌های اسپرماتوژنیک بود. از طرف دیگر در سلول‌های سرتولی، میتوکندری های در حالت تخریب قابل تشخیص بودند. اما در گروه آزمایش 2، شرایط تا اندازه ای شبیه گروه کنترل بود. نتایج مطالعه حاضر نشان داد که سترورلیکس می‌تواند اپی‌تلیوم ژرمینال را تا حدودی از اثرات سوء سیکلوفسفامید حفاظت کند.
المصادر:
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Bustos-Obregon, E., Carvallo, M., Hartley-Belmar, R., Sarabia, L. and Ponce, C. (2007). Histopathological and histometrical assessment of boron exposure effects on mouse spermatogenesis. International Journal of Morphology, 25(4): 919-925.
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Hild, S.A., Meistrich, M.L., Blye, R.P. and Reel, J.R. (2001). Lupron depot prevention of antispernatogenic/antifertility activity of the indenopyridine, CDB-4022 in the rat. Biology of Reproduction, 65(1): 165-172.
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Krüger-Genge, A., Steinbrecht, S., Küpper, J.H., Lendlein, A. and Jung, F. (2018). Evidence for cytostatic effect of cyclophosphamide on human vein endothelial cells in cancer therapy: Preliminary in vitro results. Clinical Hemorheology and Microcirculation, 69(1-2): 267-276.
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Aich, S. and Manna, C.K. (2001). Histo physiological changes of the testicular tissue due to busulphan administration in the wild Indian. Acta Biologica Hungarica, 52(1): 105-116.
Allan, C.M., Garcia, A., Spaliviero, J., Zhang, F.D. and Jimenz, M. (2004). Complete sertoli cell proliferation induced by follicle stimulation hormone (FSH) Independently of luteinizing hormone activity: Evidence from genetic models of isolated FSH action. Endocrinology, 145(4): 1587-1593.
Andriana, B.B., Tay, T.W., Maki, I., Awal, M.A., Kanai, Y., Kurohmaru, M., et al. (2004). An ultra structural study on cytotoxic effect of mono (2-ethlyhexyl) phthalate (MEHP) on testes in Shiba goat in vitro.Journal of Veterinary Science, 5(3): 235-240.
Bakhtiary, Z., Shahrooz, R., Ahmadi, A. and Soltanalinejad, F. (2020). Protective effect of ethyl pyruvate on testicular histology and fertilization potential in cyclophosphamide treated mice. Veterinary Research Forum, 11(1): 7-13.
Bhattacharya, I., Basu, S., Pradhan, B.S., Sarkar, H., Nagarajan, P. and Majumdar, S.S. (2019). Testosterone augments FSH signaling by upregulating the expression and activity of FSH-Receptor in Pubertal Primate Sertoli cells. Molecular and Cellular Endocrinology, 482: 70-80.
Bustos-Obregon, E., Carvallo, M., Hartley-Belmar, R., Sarabia, L. and Ponce, C. (2007). Histopathological and histometrical assessment of boron exposure effects on mouse spermatogenesis. International Journal of Morphology, 25(4): 919-925.
Cao, Y., Wang, X., Li, S., Wang, H., Yu, L. and Wang, P. (2017). The effects of l-carnitine against cyclophosphamide-induced injuries in mouse testis. Basic Clinical Pharmacology Toxicology, 120(2): 152-158.
Charak, B.S., Gupta, R., Mandrekar, P., Sheth, N.A., Banavali, S.D., Saikia, T.K., et al. (1990). Testicular dysfunction after cyclophosphamide-vincristine procarbazine-prednisolone chemotherapy for advanced Hodgkin’s disease. A long-term follow-up study. Cancer, 65(9): 1903-1906.
Cook, T. and Sheridan, W.P. (2000). Development of GnRH antagonists for prostate cancer: new approaches to treatment. Oncologist, 5(2): 162-168.
de Jong, W.K., Groen, H.J., Koolen, M. G., Biesma, B., Willems, L.N., Kwa, H.B., et al. (2007). Phase III study of cyclophosphamide, doxorubicin, and etoposide compared with carboplatin and paclitaxel in patients with extensive disease small-cell lung cancer. European Journal of Cancer, 43(16): 2345-2350.
Dere, E., Anderson, L.M., Hwang, K. and Boekelheide, K. (2013). Biomarkers of chemotherapy-induced testicular damage. Fertility and Sterility, 100(5): 1192-1202.
DeVita, V.T., Rosenberg, S.A. and Lawrence, T.L. (2018). Cancer: Principles and Practice of Oncology. 11th ed., Philadelphia: Lippincott, pp: 1533-1577.
Drumond, A.L., Weng, C.C., Wang, G., Chiarini-Garcia, H., Eras-Garcia, L. and Meistrich, M.L. (2011). Effects of multiple doses of cyclophosphamide on mouse testes: accessing the germ cells lost, and the functional damage of stem cells. Reproductive Toxicology, 32(4): 395-406.
Elangovan, N., Chiou, T.J., Tzeng, W.F., and Chu, S.T. (2006). Cyclophosphamide treatment causes impairment of sperm and its fertilizing ability in mice. Toxicology, 222(1-2): 60-70.
El-Awady, R.A., Semreen, M.H., Saber-Ayad, M.M., Cyprian, F., Menon, V. and Al-Tel, T.H. (2016). Modulation of DNA damage response and induction of apoptosis mediates synergism between doxorubicin and anew imidazopyridine derivative in breast and lung cancer cells. DNA Repair (Amst), 37: 1-11.
Francavilla, S.P., D Abrizio, P., Cordeschi, G., Pelliccione, F., Necozione, S., Ulisse, S., et al. (2002). Fas expression correlates with human germ cell degeneration in meiotic and post-meiotic arrest of spermatogenesis. Molecular Human Reproduction, 8(3): 213-220.
Glode, L.M., Robinson, J. and Gould, S.F. (1981). Protection from CP induced testicular damage with an analogue of gonadotrophin-releasing hormone. Lancet, 1(8230): 1132-1136.
Gründker, C., Schlotawa, L., Viereck, V., Eicke, N., Horst, A., Kairies, B., et al. (2004). Antiproliferative effects of the GnRH antagonist cetrorelix and of GnRH-II on human endometrial and ovarian cancer cells are not mediated through the GnRH type I receptor. European Journal of Endocrinology, 151(1): 141-149.
Harel, S., Fermé, C. and Poirot, C. (2011). Management of fertility in patients treated for Hodgkin’s lymphoma. Haematologica, 96(11): 1692-9169.
Haywood, H., Spaliviero, J., Jimenz, M., King, N.J., Handelsman, D.J. and Allan, C.M. (2003). Sertoli cell and germ cell development in hypogonadal mice (hpg) expressing transgenic follicle stimulating hormone alone or in combination with resttosterone. Endocrinology, 144(2): 509-517.
Hild, S.A., Meistrich, M.L., Blye, R.P. and Reel, J.R. (2001). Lupron depot prevention of antispernatogenic/antifertility activity of the indenopyridine, CDB-4022 in the rat. Biology of Reproduction, 65(1): 165-172.
Hou, M., Chrysis, D., Nurmio, M., Parvinen, M., Eksborg. S., Söder, O., et al. (2005). Doxorubicin induces apoptosis in germ line stem cells in the immature rat testis and amifostin can not protect against this cytotoxicity. Cancer Research, 65(21): 9999-10005.
Howell, S.J. and Shatel, S.M. (2005). Spermatogenesis after cancer treatment: Damage and Recovery.Journal of the National Cancer Institute Monographs, 34: 12-17.
Jonat, W., Kaufmann, M., Sauerbrei, W., Blamey, R., Cuzick, J., Namer, M., et al. (2002) Goserelin versus CP, methotrexate and fluorouracil as adjuvant therapy in premenopausal patients with node-positive breast cancer: The Zoladex Early Breast Cancer Research Association Study. Journal of Clinical Oncology, 20(24): 4628-4635.
Kaffashielahi, R. (2013). Protective effects of Resveratrol against chemotherapy drug cisplatin induced hepatotoxicity in the rat. Journal of Veterinary Clinical Pathology, 7(4): 286-299. [In Persian]
Kenney, L.B., Laufer, M.R., Grant, F.D., Grier, H. and Diller, L. (2001). High risk of infertility and long term gonadal damage in males treated with high dose cyclophosphamide for sarcoma during childhood. Cancer, 91(3): 613-621.
Krüger-Genge, A., Steinbrecht, S., Küpper, J.H., Lendlein, A. and Jung, F. (2018). Evidence for cytostatic effect of cyclophosphamide on human vein endothelial cells in cancer therapy: Preliminary in vitro results. Clinical Hemorheology and Microcirculation, 69(1-2): 267-276.
Li, D.J., Xu, Z.S., Zhang, Z.H. and Huang, Q.Y. (2006). Antagonistic effects of vitamin E on the testicular injury by cyclophosphamide in mice. Zhonghua Nan Ke Xue, 12(4): 318-322.
Meistrich, M.L. and Shetty, G. (2003). Inhibition of spermatogonial differentiation by testosterone. Journal of Andrology, 24(2): 135-148.
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