تعیین گروه سرمی و الگوی مقاومت آنتی‌بیوتیکی سالمونلاهای جداشده از طیور صنعتی تخمگذار منطقه تبریز
الموضوعات :
مختار سابقی
1
,
یونس انزابی
2
1 - دانش‌آموخته دکترای حرفه‌ای دامپزشکی، دانشکده دامپزشکی، واحد تبریز، دانشگاه آزاد اسلامی، تبریز، ایران.
2 - استادیار گروه پاتوبیولوژی، دانشکده دامپزشکی، واحد تبریز، دانشگاه آزاد اسلامی، تبریز، ایران.
تاريخ الإرسال : 19 الأحد , جمادى الثانية, 1440
تاريخ التأكيد : 13 السبت , رمضان, 1440
تاريخ الإصدار : 20 الثلاثاء , ذو القعدة, 1440
الکلمات المفتاحية:
تبریز,
سالمونلا,
گروه سرمی,
مقاومت آنتی‌بیوتیکی,
طیور تخمگذار,
ملخص المقالة :
سالمونلوزیسیکیازمهم ترینبیماری هایمشترک بینانسانوحیواناتمی باشد.استفادهاز انواعآنتی بیوتیک هاراهکار اصلی ومهمبرایکاهشمیزانبروزوتلفاتمرتبطباعفونت هایسالمونلاییاست،امامصرفنادرستوبی رویهآن هادرمزارعطیورصنعتیممکناستمنجربه پیدایشمقاومت های آنتی بیوتیکیودرنتیجهناکارآمدیداروهایضدمیکروبیشود.همچنین،انتقالسویه هایمقاومبهآنتی بیوتیک هابهانسانازطریقزنجیرهغذایی هممی تواندتهدیدیبرایبهداشتعمومیباشد.لذا هدفازاینمطالعهتعیینگروهسرمیوالگویمقاومتآنتی بیوتیکیدر سالمونلاهایجداشدهاز8گله طیور تخم گذاردر منطقه تبریزبود.بدین منظور پس از جداسازی سالمونلا از نمونه های مختلف طیور تخمگذار صنعتی، جدایه هابااستفادهازآنتی سرم هایاختصاصیازنظرگروهسرمیو سپسبه روشانتشاردیسک در آگار (Kirby-Bauer)ازنظرمقاومتنسبتبه 6 نوع آنتی بیوتیکرایجدرصنعتمرغداری کشور ایران(انروفلوکساسین، فلورفنیکل،فسفومایسین،لینکوسپکتین،سولتریموداکسی سایکلین)و 6 نوع آنتی بیوتیک مورد مصرفانسانی (جنتامایساین ،کوآموکسی‌کلاو،سیپروفلوکساسین، سفالکسین،سفوتاکسیموسفتری آکسون)بررسیشدند. از مجموع 96 نمونه مورد آزمایش، تعداد16 جدایهسالمونلا شناسائی گردید که 10 جدایهمتعلق به گروه سرمی D بودند ولی 6 جدایه در هیچ یک از گروه های سرمی مورد آزمایش قرار نداشتند. همچنینهمهجدایه ها نسبتبهجنتامایسین وفسفومایسینحساسبودند وبیشترینمیزانمقاومت هم دربرابرداکسی‌سایکلین با فراوانی35/83 درصد مشاهدهشد.شیوع نسبتاًبالایمقاومتداروییدرمیانسالمونلاهایجداشدهازطیور تخم گذار صنعتی نشاندادکهتجویزآنتی بیوتیک هابایدبااحتیاط و دقتبیشتریصورتگیرد.
المصادر:
Akbarian, R., Peighambari, S.M., Morshed, R. and Yazdani, A. (2012). Survey of Salmonella infection in Iranian poultry flocks. Iranian Veterinary Journal, 8(3): 5-10. [In Persian]
Akbarmehr, J., Zahraei Salehi, T. and Nikbakht, G.H. (2010). Identification of Salmonella isolated from poultry by m-PCR technique and evaluation of their hsp groEL gene diversity based on the PCR-RFLP analysis. African Journal of Microbiology Research, 4(15): 1599-1604.
Bogaard, A.E., London, N., Driessen, C. and Stobberingh, E.E. (2001). Antibiotic resistance of fecal Escherichia coli in poultry, poultry farmers and poultry slaughterers. Journal of Antimicrobial Chemotherapy, 47(6): 763-771.
Clinical and Laboratory Standards Institute (CLSI) (2015). Performance standards for antimicrobial susceptibility testing; 23th Informational Supplement, M100-S25, CLSI document. Clinical and Laboratory Standards Institute, Wayne, Pennsylvania, pp: 44-50.
Dreesen, D.W., Barnhart, H.M., Burke, J.L., Chen, T. and Johnson, D.C. (1992). Frequency of Salmonella enteritidis and other Salmonella in the ceca of spent hens at time of slaughter. Avian Diseases, 36(2): 247-250.
Ezatpanah, E., Moradi Bidhendi, S., Khaki, P., Ghaderi, R., Seyedan Jasbi, E. and Moghtadaee Far, S. (2013). Isolation, serotyping and antibiotic-resistance pattern of isolated salmonella from chicken of Arak. Iranian Veterinary Journal, 9(2): 88-96. [In Persian]
Firoozeh, F., Shahcheraghi, F.E., Zahraei Salehi, T., Karimi, V. and Aslani, M.M. (2011). Antimicrobial resistance profile and presence of class I integrongs among Salmonella enterica serovars isolated from human clinical specimens in Tehran, Iran. Iranian Journal of Microbiology, 3(3): 112-117.
Garcia, C., Soriano, J.M., Benítez, V. and Catalá-Gregori, P. (2011). Assessment of Salmonella spp. in feces, cloacal swabs, and eggs (eggshell and content separately) from a laying hen farm. Poultry Science, 90(7): 1581-1585.
Gast, R.K. (2013). Paratyphoid infections. In: Diseases of Poultry. Swayne, D.E., Glisson, J.R., McDougald, L.R., Nolan, L.K., Saurez, D.L. and Nair, V. editors. 13th ed., John Wiley and Sons, Inc, pp: 693-706.
Grimont, P.A.D. and Weill, F.X. (2007). Antigenic formulae of the Salmonella serovars. 9th ed., WHO Collaborating Center for Reference and Research on Salmonella, Institute Pasteur, France, Paris, pp: 1-166.
Guibourdenche, M., Roggentin, P., Mikoletit, M., Fields, P.I., Bockemuhl, J., Grimont, P.A.D., et al. (2010). Supplement 2003-2007(No.47) to the White-Kauffmann-Le Minor scheme. Research Microbiology, 161(1): 26-29.
Hopper, S.A. and Mawer, S. (1988). Salmonella enteritidis in a commercial layer flock. Veterinary Record, 123(13): 351.
Jamshidi, A., Zahraei Salehi, T. and Afshari Nik, S. (2007). Detection of Salmonella spp contamination of carcasses slaughtered in poultry abattoir in Mashhad, Iran. Archives of Razi Institute, 62(4): 229-233.
Khakhria, R., Duck, D. and Lior, H. (1991). Distribution of Salmonella enteritidis phage types in Canada. Epidemiology and Infection, 106 (1): 25-32.
Mezal, E.H., Sabol, A., Khan, M.A., Ali, N., Stefanova, R. and Khan, A.A. (2014). Isolation and molecular characterization of Salmonella enterica serovar enteritidis from poultry house and clinical samples during 2010. Food Microbiology, 38(1): 67-74.
Morshed, R. and Peighambari, S.M. (2010). Salmonella infections in poultry flocks in the vicinity of Tehran. International Journal of Veterinary Research, 4(4): 273-276.
Morshed, R. (2013). Bacteriological study of broiler flocks (Salmonella contamination) in Amol city. Veterinary Journal, 25(4): 23-28. [In Persian]
Nadine, B., Lieve, H. and Rijpens, N. (2004). Phenotypic and molecular typing of Salmonella strains reveals different contamination sources in two commercial pig slaughterhouses. Applied and Environmental Microbiology, 70(9): 5305-5314.
Parry, C.M., Tinh Hien, T., Dougan, G., White, N.J. and Farrar, J.J. (2002). Typhoid fever. New England Journal of Medicine, 347(22): 1770-1782.
Quinn, P.J., Markey, B.K., Carter, M.E., Donnelly, W.J. and Leonard, F.C. (2002). Veterinary Microbiology and Microbial Disease. Blakwell Publishing, pp: 109-118.
Tabatabayi, A.H. and Firouzi, R. (2002). Diseases of animals due to Bacteria. 1st ed. Tehran University Press, Tehran, pp: 232-234. [In Persian]
Waltman, W.D. and Gast, R.K. (2008). Sallmonellosis. In: A Laboratory Manual for the Isolation, Identification and Characterization of Avian Pathogens. Dufour-Zavala, L., Swayne, D.E., Glisson, J.R., Pearson, J.E., Reed, W.M., Jackwood, M.W. and Woolcock, P.R. editors. 5th ed., American Association of Avian Pathologists, Athens, GA, pp: 3-10.
Zahraei Salehi, T. (1999). Salmonella. 1st ed., Iran: University of Tehran, pp: 1-97. [In Persian]
Zahraei Salehi, T., Mahzounieh, M. and Saeedzadeh, A. (2005). The isolation of antibiotic-resistant Salmonella from intestine and liver of poultry in Shiraz province of Iran. International Journal of Poultry Science, 4(5): 320-322.
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Akbarian, R., Peighambari, S.M., Morshed, R. and Yazdani, A. (2012). Survey of Salmonella infection in Iranian poultry flocks. Iranian Veterinary Journal, 8(3): 5-10. [In Persian]
Akbarmehr, J., Zahraei Salehi, T. and Nikbakht, G.H. (2010). Identification of Salmonella isolated from poultry by m-PCR technique and evaluation of their hsp groEL gene diversity based on the PCR-RFLP analysis. African Journal of Microbiology Research, 4(15): 1599-1604.
Bogaard, A.E., London, N., Driessen, C. and Stobberingh, E.E. (2001). Antibiotic resistance of fecal Escherichia coli in poultry, poultry farmers and poultry slaughterers. Journal of Antimicrobial Chemotherapy, 47(6): 763-771.
Clinical and Laboratory Standards Institute (CLSI) (2015). Performance standards for antimicrobial susceptibility testing; 23th Informational Supplement, M100-S25, CLSI document. Clinical and Laboratory Standards Institute, Wayne, Pennsylvania, pp: 44-50.
Dreesen, D.W., Barnhart, H.M., Burke, J.L., Chen, T. and Johnson, D.C. (1992). Frequency of Salmonella enteritidis and other Salmonella in the ceca of spent hens at time of slaughter. Avian Diseases, 36(2): 247-250.
Ezatpanah, E., Moradi Bidhendi, S., Khaki, P., Ghaderi, R., Seyedan Jasbi, E. and Moghtadaee Far, S. (2013). Isolation, serotyping and antibiotic-resistance pattern of isolated salmonella from chicken of Arak. Iranian Veterinary Journal, 9(2): 88-96. [In Persian]
Firoozeh, F., Shahcheraghi, F.E., Zahraei Salehi, T., Karimi, V. and Aslani, M.M. (2011). Antimicrobial resistance profile and presence of class I integrongs among Salmonella enterica serovars isolated from human clinical specimens in Tehran, Iran. Iranian Journal of Microbiology, 3(3): 112-117.
Garcia, C., Soriano, J.M., Benítez, V. and Catalá-Gregori, P. (2011). Assessment of Salmonella spp. in feces, cloacal swabs, and eggs (eggshell and content separately) from a laying hen farm. Poultry Science, 90(7): 1581-1585.
Gast, R.K. (2013). Paratyphoid infections. In: Diseases of Poultry. Swayne, D.E., Glisson, J.R., McDougald, L.R., Nolan, L.K., Saurez, D.L. and Nair, V. editors. 13th ed., John Wiley and Sons, Inc, pp: 693-706.
Grimont, P.A.D. and Weill, F.X. (2007). Antigenic formulae of the Salmonella serovars. 9th ed., WHO Collaborating Center for Reference and Research on Salmonella, Institute Pasteur, France, Paris, pp: 1-166.
Guibourdenche, M., Roggentin, P., Mikoletit, M., Fields, P.I., Bockemuhl, J., Grimont, P.A.D., et al. (2010). Supplement 2003-2007(No.47) to the White-Kauffmann-Le Minor scheme. Research Microbiology, 161(1): 26-29.
Hopper, S.A. and Mawer, S. (1988). Salmonella enteritidis in a commercial layer flock. Veterinary Record, 123(13): 351.
Jamshidi, A., Zahraei Salehi, T. and Afshari Nik, S. (2007). Detection of Salmonella spp contamination of carcasses slaughtered in poultry abattoir in Mashhad, Iran. Archives of Razi Institute, 62(4): 229-233.
Khakhria, R., Duck, D. and Lior, H. (1991). Distribution of Salmonella enteritidis phage types in Canada. Epidemiology and Infection, 106 (1): 25-32.
Mezal, E.H., Sabol, A., Khan, M.A., Ali, N., Stefanova, R. and Khan, A.A. (2014). Isolation and molecular characterization of Salmonella enterica serovar enteritidis from poultry house and clinical samples during 2010. Food Microbiology, 38(1): 67-74.
Morshed, R. and Peighambari, S.M. (2010). Salmonella infections in poultry flocks in the vicinity of Tehran. International Journal of Veterinary Research, 4(4): 273-276.
Morshed, R. (2013). Bacteriological study of broiler flocks (Salmonella contamination) in Amol city. Veterinary Journal, 25(4): 23-28. [In Persian]
Nadine, B., Lieve, H. and Rijpens, N. (2004). Phenotypic and molecular typing of Salmonella strains reveals different contamination sources in two commercial pig slaughterhouses. Applied and Environmental Microbiology, 70(9): 5305-5314.
Parry, C.M., Tinh Hien, T., Dougan, G., White, N.J. and Farrar, J.J. (2002). Typhoid fever. New England Journal of Medicine, 347(22): 1770-1782.
Quinn, P.J., Markey, B.K., Carter, M.E., Donnelly, W.J. and Leonard, F.C. (2002). Veterinary Microbiology and Microbial Disease. Blakwell Publishing, pp: 109-118.
Tabatabayi, A.H. and Firouzi, R. (2002). Diseases of animals due to Bacteria. 1st ed. Tehran University Press, Tehran, pp: 232-234. [In Persian]
Waltman, W.D. and Gast, R.K. (2008). Sallmonellosis. In: A Laboratory Manual for the Isolation, Identification and Characterization of Avian Pathogens. Dufour-Zavala, L., Swayne, D.E., Glisson, J.R., Pearson, J.E., Reed, W.M., Jackwood, M.W. and Woolcock, P.R. editors. 5th ed., American Association of Avian Pathologists, Athens, GA, pp: 3-10.
Zahraei Salehi, T. (1999). Salmonella. 1st ed., Iran: University of Tehran, pp: 1-97. [In Persian]
Zahraei Salehi, T., Mahzounieh, M. and Saeedzadeh, A. (2005). The isolation of antibiotic-resistant Salmonella from intestine and liver of poultry in Shiraz province of Iran. International Journal of Poultry Science, 4(5): 320-322.
