نقش شاخصه‌های ژنتیکی در تعیین نژاد اسب‌های عرب
الموضوعات :
سروین جباری
1
(دانش‌آموخته کارشناسی ارشد ژنتیک، دانشکده علوم پایه، واحد تبریز، دانشگاه آزاد اسلامی، تبریز، ایران.)
محمدرضا مشایخی
2
(استادیار گروه ژنتیک، دانشکده علوم پایه، واحد تبریز، دانشگاه آزاد اسلامی، تبریز، ایران.)
علی حسن پور
3
(دانشیار گروه علوم درمانگاهی، دانشکده دامپزشکی، واحد تبریز، دانشگاه آزاد اسلامی، تبریز، ایران.)
تاريخ الإرسال : 05 الثلاثاء , ربيع الأول, 1440
تاريخ التأكيد : 09 الأربعاء , شوال, 1440
تاريخ الإصدار : 20 الثلاثاء , ذو القعدة, 1440
الکلمات المفتاحية:
تنوع ژنتیکی,
اسب عرب,
ریز‌ماهواره,
توالی تکراری کوتاه,
ملخص المقالة :
با توجه به این که صنعت پرورش اسب به عنوان چهارمین صنعت از لحاظ درآمدزایی در جهان مطرح است و نیز با عنایت به تنوع گونه‌های اسب‌های اصیلدر جهان و ضرورت تعیین خلوص و نسب نزد صاحبان و پرورش‌دهندگان اسب، اهمیت تحقیقات آزمایشگاهی در این مورد مشخص می گردد. با توجه به تحقیقات گذشته در داخل و خارج کشور که به صورت موردی انجام شده، خلأ موجود در بررسی اسب های ایران از لحاظ تنوع مارکر‌های ژنتیکی مثل توالی تکراری کوتاه (short tandem repeats; STR) ضرورت پیدا می‌کند. دراینمطالعه، تنوعژنتیکی50رأس اسبنژادعربمورد بررسیقرار گرفت.برایاینمنظورازنشانگرهایریز‌ماهوارهپیشنهادی ISAG(International Society for Animal Genetics) استفادهشد.ایننشانگرهاشاملریز‌ماهواره‌هایVHL20، HTG4، HMS7 و AHT4می‌باشند.برای این منظور DNA ژنومی از نمونه خونی اسب ها توسط روش مایلر استخراج گردید و به وسیله واکنش زنجیره‌ای پلیمراز چندگانه توسط پرایمر فلورسنت تکثیر شد. داده‌ها نشان دادکهتنوعژنتیکیبالاییدرجمعیتاسب هایعربوجوددارد.تعدادآلل‌هایمشاهده شدهبرایهرجایگاهاز6تا9آلل متغیربودومارکرهای AHT4 و VHL20با9آللدارایبیشترینتعدادآللو همچنین HTG4بیشترین مقدار هتروزیگوسیتی را دارا بود. همچنینجایگاه HMS7دارای7آللبودکهکمترینتعدادآللدرمیانجایگاه‌هایبررسی شدهراداشتوجایگاه VHL20دارایپایین‌ترینمقدار هتروزیگوسیتی بود.نتایجحاصلهازمطالعه حاضرنشان دهندهفراوانیبالایتنوعژنتیکیجمعیتاسب‌های عرببود.
المصادر:
Ala-Amjadi, M., Yeganeh, H. and Sadeghi, M. (2017). Study of genetic variation in Iranian Kurdish horse using microsatellite marker. Iranian Journal of Animal Science, 48(3): 335-342. [In Persian]
Banaabadi, H., Mashayekhi, M.R., Hasanpour, A. and Ayubi, M.R. (2017). The study of genetic variability of Arabian and Caspian horses using microsatellite. Journal of Animal Science Researches, 27(3): 175-183. [In Persian]
Binns, M. and Holmes, N. (1995). The identification of polymorphic microsatellite loci in the horse and their use in thoroughbred parentage testing. British Veterinary Journal, 151(1): 9-15.
Botstein, D. and White, R.L. (1980). Construction of a genetic linkage map in man using restriction fragment length polymorphisms. American Journal of Human Genetics, 32(3): 314-331.
Bowling, A. (2001). Historical development and application of molecular genetic tests for horse identification and parentage control. Livestock Production Science, 72(1-2): 111-116.
Chowdhary B.P. and Raudsepp, T. (2000). Cytogenetics and physical gene maps. In: The Genetics of The Horse. Bowling A.T. and Ruvinsky A. editors., New York: CABI Publishing, pp:171-242.
Ellegren, H., Johansson, M., Sandberg, K. and Andersson, L. (1992). Cloning of highly polymorphic microsatellites in the horse. Animal Genetics, 23(2): 133-142.
Evans, A.R. and Janis, C.M. (2014). The evolution of high dental complexity in the horse lineage. Annales Zoologici Fennici, BioOne, 51(1): 73-79.
Fornal, A., Radko, A. and Piestrzyńska-Kajtoch, A. (2013). Genetic polymorphism of Hucul horse population based on 17 microsatellite loci. Acta Biochimica Polonica, 60(4): 761-765.
Ghaheri, M., Kahrizi, D., Yari, K., Babaie, A., Suthar, R.S. and Kazemi, E. (2016). A comparative evaluation of four DNA extraction protocols from whole blood sample. Cellular and Molecular Biology, 62(3): 120-124.
Grubb, P. (2005). Order Perissodactyla, Mammals Species of the World, A Taxonomic and Geographic Reference. 3rd ed., The Johns Hopkins University Press, Baltimore, pp: 629-636.
Guekin, G., Bertaud, M. and Amigues, Y. (1994). Characterization of seven new horse microsatellites: HMS1, HMS2, HMS3, HMS5, HMS6, HMS7 and HMS8. Animal Genetics, 25(1): 62-62.
Kavar, T., Habe, F., Brem, G. and Dovč, P. (1999). Mitochondrial D‐loop sequence variation among the 16 maternal lines of the Lipizzan horse breed. Animal Genetics, 30(6): 423-430.
Kimpton, C., Fisher, D., Watson, S., Adams, M. Urquhart, A., Lygo, J., et al. (1994). Evaluation of an automated DNA profiling system employing multiplex amplification of four tetrameric STR loci. International Journal of Legal Medicine, 106(6): 302-311.
Lee, S.Y. and Cho, G.J. (2006). Parentage testing of Thoroughbred horse in Korea using microsatellite DNA typing. Journal of Veterinary Science, 7(1): 63-67.
Luís, C., Bastos-Silveira, C., Gus Cothran, E. and Do Mar Oom, M. (2006). Iberian origins of New World horse breeds. Journal of Heredity, 97(2): 107-113.
Melnyk, O.V., Spyrydonov, V.G., Dzitsiuk, V.V. and Osadchyj, S.A. (2013). Molecular-Genetic CHaracterization of Shetland pony using microsatellite loci of DNA. The Animal Biology, 15(4): 73-79.
Micka, K.A., Sprecher, C., Lins, A., Comey, C., Koons, B., Crouse, C., et al. (1996). Validation of multiplex polymorphic STR amplification sets developed for personal identification applications, Journal of Forensic Science, 41(4): 582-590.
Miller, S., Dykes, D. and Polesky, H. (1988). A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Research, 16(3): 1215-1216.
Seyedabadi, H., Amirinia, C., Banabazi, M.H. and Emrani, H. (2006). Parentage verification of Iranian Caspian horse using microsatellites markers. Iranian Journal of Biotechnology, 4(4): 260-264.
Shelyov, A., Melnyk, O., Suprun, I., Spyrydonov, V., Melnychvk, S., Dzitsiuk, V., et al. (2014). The comparative analysis of the allele pool of Thoroughbred horses in different countries. Iranian Journal of Applied Animal Science, 4(3): 637-641.
Solis, A., Jugo, B., Mériaux, J., Iriondo, M., Mazón, L., Aguirre, A., et al. (2005). Genetic diversity within and among four South European native horse breeds based on microsatellite DNA analysis: implications for conservation. Journal of Heredity, 96(6): 670-678.
Vahdani, M.A., Mashayekhi, M.R., Hasan pour, A. and Ayubi, M.R. (2016). Evaluation of the genetic diversity of Iranian Kurdish horses. Animal Science Research (Agricultural Knowledge), 27(1): 95-102. [In Persian]
Van Haeringen, H., Bowling, A., Stott, M., Lenstra, J. and Zwaagstra, K. (1994). A highly polymorphic horse microsatellite locus: VHL20. Animal Genetics, 25(3): 207-207.
Vilà, C., Leonard, J.A., Götherström, A., Marklund, S., Sandberg, K., Lidén, K., et al. (2001). Widespread origins of domestic horse lineages. Science, 291(5503): 474-477.
Weinstock, J., Willerslev, E., Sher, A., Tong, W., Y.W Ho, S., Rubenstein, D., et al. (2005). Evolution, systematics, and phylogeography of Pleistocene horses in the New World: a molecular perspective. PLoS Biology, 3(8): 241-248.
Wimmers, K., Ponsuksili, S., Schmoll, F., Hardge, T., Hatzipanagiotou, A., Weber, J., et al. (1998). Efficiency of microsatellite markers of the international standard panel for parentage control in German horse populations. Zuechtungskunde (Germany), 70(4): 233-241.
_||_
Ala-Amjadi, M., Yeganeh, H. and Sadeghi, M. (2017). Study of genetic variation in Iranian Kurdish horse using microsatellite marker. Iranian Journal of Animal Science, 48(3): 335-342. [In Persian]
Banaabadi, H., Mashayekhi, M.R., Hasanpour, A. and Ayubi, M.R. (2017). The study of genetic variability of Arabian and Caspian horses using microsatellite. Journal of Animal Science Researches, 27(3): 175-183. [In Persian]
Binns, M. and Holmes, N. (1995). The identification of polymorphic microsatellite loci in the horse and their use in thoroughbred parentage testing. British Veterinary Journal, 151(1): 9-15.
Botstein, D. and White, R.L. (1980). Construction of a genetic linkage map in man using restriction fragment length polymorphisms. American Journal of Human Genetics, 32(3): 314-331.
Bowling, A. (2001). Historical development and application of molecular genetic tests for horse identification and parentage control. Livestock Production Science, 72(1-2): 111-116.
Chowdhary B.P. and Raudsepp, T. (2000). Cytogenetics and physical gene maps. In: The Genetics of The Horse. Bowling A.T. and Ruvinsky A. editors., New York: CABI Publishing, pp:171-242.
Ellegren, H., Johansson, M., Sandberg, K. and Andersson, L. (1992). Cloning of highly polymorphic microsatellites in the horse. Animal Genetics, 23(2): 133-142.
Evans, A.R. and Janis, C.M. (2014). The evolution of high dental complexity in the horse lineage. Annales Zoologici Fennici, BioOne, 51(1): 73-79.
Fornal, A., Radko, A. and Piestrzyńska-Kajtoch, A. (2013). Genetic polymorphism of Hucul horse population based on 17 microsatellite loci. Acta Biochimica Polonica, 60(4): 761-765.
Ghaheri, M., Kahrizi, D., Yari, K., Babaie, A., Suthar, R.S. and Kazemi, E. (2016). A comparative evaluation of four DNA extraction protocols from whole blood sample. Cellular and Molecular Biology, 62(3): 120-124.
Grubb, P. (2005). Order Perissodactyla, Mammals Species of the World, A Taxonomic and Geographic Reference. 3rd ed., The Johns Hopkins University Press, Baltimore, pp: 629-636.
Guekin, G., Bertaud, M. and Amigues, Y. (1994). Characterization of seven new horse microsatellites: HMS1, HMS2, HMS3, HMS5, HMS6, HMS7 and HMS8. Animal Genetics, 25(1): 62-62.
Kavar, T., Habe, F., Brem, G. and Dovč, P. (1999). Mitochondrial D‐loop sequence variation among the 16 maternal lines of the Lipizzan horse breed. Animal Genetics, 30(6): 423-430.
Kimpton, C., Fisher, D., Watson, S., Adams, M. Urquhart, A., Lygo, J., et al. (1994). Evaluation of an automated DNA profiling system employing multiplex amplification of four tetrameric STR loci. International Journal of Legal Medicine, 106(6): 302-311.
Lee, S.Y. and Cho, G.J. (2006). Parentage testing of Thoroughbred horse in Korea using microsatellite DNA typing. Journal of Veterinary Science, 7(1): 63-67.
Luís, C., Bastos-Silveira, C., Gus Cothran, E. and Do Mar Oom, M. (2006). Iberian origins of New World horse breeds. Journal of Heredity, 97(2): 107-113.
Melnyk, O.V., Spyrydonov, V.G., Dzitsiuk, V.V. and Osadchyj, S.A. (2013). Molecular-Genetic CHaracterization of Shetland pony using microsatellite loci of DNA. The Animal Biology, 15(4): 73-79.
Micka, K.A., Sprecher, C., Lins, A., Comey, C., Koons, B., Crouse, C., et al. (1996). Validation of multiplex polymorphic STR amplification sets developed for personal identification applications, Journal of Forensic Science, 41(4): 582-590.
Miller, S., Dykes, D. and Polesky, H. (1988). A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Research, 16(3): 1215-1216.
Seyedabadi, H., Amirinia, C., Banabazi, M.H. and Emrani, H. (2006). Parentage verification of Iranian Caspian horse using microsatellites markers. Iranian Journal of Biotechnology, 4(4): 260-264.
Shelyov, A., Melnyk, O., Suprun, I., Spyrydonov, V., Melnychvk, S., Dzitsiuk, V., et al. (2014). The comparative analysis of the allele pool of Thoroughbred horses in different countries. Iranian Journal of Applied Animal Science, 4(3): 637-641.
Solis, A., Jugo, B., Mériaux, J., Iriondo, M., Mazón, L., Aguirre, A., et al. (2005). Genetic diversity within and among four South European native horse breeds based on microsatellite DNA analysis: implications for conservation. Journal of Heredity, 96(6): 670-678.
Vahdani, M.A., Mashayekhi, M.R., Hasan pour, A. and Ayubi, M.R. (2016). Evaluation of the genetic diversity of Iranian Kurdish horses. Animal Science Research (Agricultural Knowledge), 27(1): 95-102. [In Persian]
Van Haeringen, H., Bowling, A., Stott, M., Lenstra, J. and Zwaagstra, K. (1994). A highly polymorphic horse microsatellite locus: VHL20. Animal Genetics, 25(3): 207-207.
Vilà, C., Leonard, J.A., Götherström, A., Marklund, S., Sandberg, K., Lidén, K., et al. (2001). Widespread origins of domestic horse lineages. Science, 291(5503): 474-477.
Weinstock, J., Willerslev, E., Sher, A., Tong, W., Y.W Ho, S., Rubenstein, D., et al. (2005). Evolution, systematics, and phylogeography of Pleistocene horses in the New World: a molecular perspective. PLoS Biology, 3(8): 241-248.
Wimmers, K., Ponsuksili, S., Schmoll, F., Hardge, T., Hatzipanagiotou, A., Weber, J., et al. (1998). Efficiency of microsatellite markers of the international standard panel for parentage control in German horse populations. Zuechtungskunde (Germany), 70(4): 233-241.
