اثرات مکملسازی جیره با سطوح مختلف آرژینین بر جمعیت میکروبی رودۀ کور و خصوصیات آنتیاکسیدانی سرم جوجههای گوشتی سالم و چالشیافته با مخلوط آیمریاهای مختلف
الموضوعات :
فاطمه ایزدی یزدان آبادی
1
,
غلامعلی مقدم
2
,
احمد نعمت اللهی
3
,
منیره خرداد مهر
4
,
مهدی عباس‏ آبادی
5
,
هادی قنبرزاده
6
1 - دانشجوی دکترای تخصصی علوم دامی، دانشکده کشاورزی، دانشگاه تبریز، تبریز، ایران.
2 - استاد گروه علوم دامی، دانشکده کشاورزی، دانشگاه تبریز، تبریز، ایران
3 - استاد گروه پاتوبیولوژی، دانشکده دامپزشکی، دانشگاه تبریز، تبرز، ایران
4 - دانشیار گروه پاتوبیولوژی ، دانشکده دامپزشکی، دانشگاه تبریز، تبریز، ایران.
5 - دانشجوی کارشناسی ارشد علوم دامی، دانشکده کشاورزی، دانشگاه بیرجند، بیرجند، ایران.
6 - دانشجوی دکترای تخصصی علوم دامی، دانشکده کشاورزی، دانشگاه بیرجند، بیرجند، ایران.
تاريخ الإرسال : 09 السبت , جمادى الأولى, 1441
تاريخ التأكيد : 19 الأحد , ذو الحجة, 1441
تاريخ الإصدار : 03 السبت , محرم, 1442
الکلمات المفتاحية:
آرژینین,
جمعیت میکروبی,
آیمریا,
جوجهگوشتی,
رودهکور,
وضعیت آنتیاکسیدانی,
ملخص المقالة :
کوکسیدیوز سالانه باعث زیان های اقتصادی در واحدهای پرورش طیور می شود و استراتژی های تغذیه ای می تواند آن را تخفیف دهد. این مطالعه به منظور بررسی اثرات سطوح مختلف آرژینین بر جمعیت میکروبی روده ء کور و خصوصیات آنتی اکسیدانی سرم جوجه های گوشتی چالش یافته با آیمریا انجام شد. تیمارهای آزمایشی شامل جوجه های سالم و جوجه های چالش یافته تغذیه شده با جیره های مکمل سازی شده با مقادیر 85، 100، 125 و 150 درصد آرژینین قابل هضم توصیه شده، بودند. برای کشت باکتریایی،درزمانکشتار (21 و 42 روزگی) ازمحتویاترودهکور تحتشرایطاستریلنمونه برداری انجام شد.همچنین سطح فاکتورهای اکسیدانی و آنتی اکسیدانی و نیتریک اکسید در سرم جوجه ها اندازه گیری شد. روده کور جوجه هایی که جیره های حاوی سطوح 125 و 150 درصد آرژینین را دریافت کرده بودند، جمعیت اشریشیای کولای پایین تر و جمعیت لاکتوباسیل ها، بیفیدوباکتری ها و توتال باکتری های بالاتر و همچنین pH بالاتری را نشان دادند (05/0p < /em><)، ولی جمعیت انتروکوکوس ها تحت تأثیر سطوح آرژینین و چالش کوکسیدیوز قرار نگرفت (05/0p < /em>>). همچنین چالش با آیمریا، سطح سرمی گلوتاتیون پراکسیداز، سوپر اکسید دیسموتاز و ظرفیت تام آنتی اکسیدانی را کاهش و سطح سرمی نیتریک اکسید و مالون دی آلدئید را افزایش داد (05/0p < /em><). البته، افزودن آرژینین تنها در سطوح 125 و 150 درصد توانست سطح گلوتاتیون پراکسیداز را افزایش دهد (05/0p < /em><) و تأثیر معنی داری بر دیگر پارامترها نداشت (05/0p < /em>>).مطالعه حاضر مشخص کرد که مصرف آرژینین در سطوح 125 و 150 درصد، تحت شرایط چالش با آیمریا، جمعیت باکتری های پاتوژن را کاهش و جمعیت باکتری های سودمند و نیز سطح گلوتاتیون پراکسیداز را افزایش داد.
المصادر:
Allen, P. (1997). Nitric oxide production during Eimeria tenella infections in chickens. Poultry Science, 76(6): 810-813.
Allen, P.C. and Fetterer, R.H. (2000). Effect of Eimeria acervulina infections on plasma L-arginine. Poultry Science, 79(10): 1414–1417.
Arak, H., Karimi Torshizi, M.A. and Rahimi, Sh. (2013). Study on the effect of Savory (Satureja khuzestanica) essential oil and Polysorb toxin-binder against experimental aflatoxicosis in Japanese quail. Veterinary Clinical Pathology, 7(27): 249-260. [In Persian]
Atakisi, O., Atakisi, E. and Kart, A. (2009). Effects of dietary zinc and L-arginine supplementation on total antioxidants capacity, lipid peroxidation, nitric oxide, egg weight and blood biochemical values in Japanese quails. Biological Trace Element Research, 132(1-3): 136-143.
Azad, M.A.K., Kikusato, T., Maekawa, H., Shirakawa, M. and Toyomizu, M. (2010). Metabolic characteristics and oxidative damage to skeletal muscle in broiler chickens exposed to chronic heat stress. Comparative Biochemistry and Physiology, 155(3): 401-406.
Boulton, K., Nolan, M. J., Harman, K., Psifidi, A., Wu, Z. and Bishop, S., (2017). Resistance and Tolerance are separabletraits in the innate immune response of chickens to Eimeria tenella induced coccidiosis. Veterinary Clinical Pathology, 44 (2): 28-35.
Bun, S.D., Guo, Y.M., Guo, F.C., Ji, F.J. and Cao, H. (2011). Influence of organic zinc supplementation on the antioxidant status and immune responses of broilers challenged with Eimeria tenella. Poultry Science, 90(6): 1220-1226.
Chandra, J., Samali, A. and Orrenius, S. (2000). Triggering and modulation of apoptosis by oxidative stress. Free Radical Biology and Medicine, 29(3-4): 323-333.
Craven, S.E. and Williams, D.D. (1998). In vitro attachment of Salmonella typhimurium to chicken cecal mucus: effect of cations andpretreatment with Lactobacillusspp. isolated from the intestinaltracts of chickens. Journal of Food Protection, 61(3): 265-271.
Dröge, W. (2002). Free radicals in the physiological control of cell function. Physiology Review, 82(1): 47-95.
Duan, X., Li, F., Mou, S., Feng, J., Liu, P. and Xu, L. (2015). Effects of dietary L-arginine on laying performance and anti-oxidant capacity of broiler breeder hens, eggs, and offspring during the late laying period. Poultry Science, 94(12): 2938-2943.
Giannenas, I., Papadopoulos, E., Tsalie, E., Triantafillou, E., Henikl, S., Teichmann, K. and Tontis, D. (2012). Assessment of dietary supplementation with probiotics on performance, intestinal morphology and microflora of chickens infected with Eimeria tenella. Veterinary Parasitology, 188(1-2): 31-40.
Graat, E.A., Ploeger, H.W., Henken, A.M., De Vries Reilingh, G., Noordhuzien, J.P. and Van Beek, P.N. (1996). Effect of initial litter contamination level with Eimeria acervulina on population dynamics and production characteristics in broilers. Veterinary Parasitolgy, 65(3-4): 223-232.
He, J., Hwang, G., Liu, Y., Gao, L., Kilpatrick-Liverman, L., Santarpia, P., et al. (2016). L-Arginine modifies the exopolysaccharide matrix and thwarts Streptococcus mutans outgrowth within mixed-species oral biofilms. Journal of Bacteriology, 198(19): 2651-2661.
Langrová, I., Chodová, D., Tůmová, T., Horáková, B., Krejčířová, R., Šašková, M., et al. (2019). Assessment of low doses of Eimeria tenella sporulated oocysts on the biochemical parameters and intestinal microflora of chickens. Turkish Journal of Veterinary and Animal Sciences, 43(1): 76-81.
Liopis, M., Antolin, M., Guarner, F., Salas, A. and Malagelada, J.R. (2005). Mucosal colonisation with Lactobacillus casei mitigates barrier injury induced by exposure to trinitronbenzene sulphonic acid. Gut, 54(7): 955-959.
Ma, X.Y.Y.C., Lin, Z.Y., Jiang, C.T., Zheng, G.L., Zhou, D.Q., Yu, T., et al. (2010). Dietary arginine supplementation enhances anti-oxidative capacity and improves meat quality of finishing pigs. Amino Acids, 38(1): 95-102.
Mahdavi, S. and Nobakht, A. (2018). Evaluation of the effect of Thyme (Thymus vulgaris L.) and Ziziphora (Ziziphora tenuior L.) essential oils on intestinal microflora of broilers. Veterinary Clinical Pathology, 11(44): 305-312. [In Persian]
Milinkovik-Thur, S., Stogective, Z., Prisljin, J., Zdelar-Tuk, M., Poljicak-Milas, N., Ljubic, B.B., et al. (2007). Effect of refeeding on the antioxidant system in cockerels and pullets. Acta Veterinaria Hungarica, 55(2): 181-188.
Montagne, L., Pluske, J.R. and Hampson, D.J. (2003). A review of interactions between dietary fiber and the intestinal mucosa, and their consequences on digestive health in young non-ruminant animals. Animal Feed Science and Technology, 108(1-4): 95-117.
Mookiah, S., Sieo, C.C., Ramasamy, K., Abdullah, N. and Ho, Y.W. (2014). Effects of dietary prebiotics, probiotic and synbiotics on performance, caecal bacterial populations and caecal fermentation concentrations of broiler chickens. Journal of Science Food Agriculture,94(2): 341-348.
Olfati, A., Mojtahedin, A., Sadeghi, T. Akbari, M. and Martínez-Pastor, F. (2018). Comparison of growth performance and immune responses of broiler chicks reared under heat stress, cold stress and thermoneutral conditions. Spanish Journal of Agriculture Research, 16(2): 1-7.
Perez, P.F., Minnaard, J., Rouvet, M., Knabenhans, C., Brassart, D., De Antoni, G.L., et al. (2001). Inhibition of Giardia intestinalis by extracellular factors from lactobacilli: an in vitro study. Applied Environment Microbiology, 67(11): 5037-5042.
Qin, Z.R., Fukata, T., Baba, E. and Arakawa, A. (1995). Effect of Eimeria tenella infection on Salmonella enteritidis infection in chickens. Poultry Science, 74(1): 1-7.
Rasoulifard, M.H. and Zargari, F. (2015). The effects of aqueous extract of white tea on serum antioxidant enzymes in rats exposed to arsenic. Veterinary Clinical Pathology, 9(34): 153-178. [In Persian]
Ren, W., Chen, S., Yin, J., Duan, J., Li, T., Liu, G., et al. (2014). Dietary arginine supplementation of mice alters the microbial population and activates intestinal innate immunity. The Journal of Nutrition, 144(6): 988-995.
Ren, W., Zou, L., Li, N., Wang, Y., Liu, G., Peng, Y., et al. (2013). Dietary arginine supplementation enhances immune responses to inactivated Pasteurella multocida vaccination in mice. British Journal of Nutrition, 109(5): 867-72.
Sergeant, M.J., Constantinidou, C., Cogan, T.A., Bedford, M.R., Penn, C.W. and Pallen, M.J. (2014). Extensive Microbial and Functional Diversity within the Chicken Cecal Microbiome, Plos One, 9(3): 18-21.
Tierney, J., Gowing, H., Van Sinderen, D., Flynn, S., Stanley, L., McHardy, N., et al. (2004). In vitro inhibition of Eimeria tenella invasion by indigenous chicken Lactobacillus species. Veterinary Parasitology, 122(3): 171-182.
Williams, R.B. (1999). A compartmentalised model for the estimation of the cost of coccidiosis to the world’s chicken production industry. International Journal of Parasitology, 29(8): 1209-1229.
Wu, G. and Meininger, C.J. (2000). Arginine nutrition and cardiovascular function. Journal of Nutrition, 130(11): 2626-2629.
Wu, Z.G., Hu, T.J., Rothwell, L., Vervelde, L., Kaiser, P., Boulton, K., et al. (2016). Analysis of the function of IL-10 in chickens using specific neutralising antibodies and a sensitive capture ELISA. Developmental and Comparative Immunology, 63(1): 206-212.
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Allen, P. (1997). Nitric oxide production during Eimeria tenella infections in chickens. Poultry Science, 76(6): 810-813.
Allen, P.C. and Fetterer, R.H. (2000). Effect of Eimeria acervulina infections on plasma L-arginine. Poultry Science, 79(10): 1414–1417.
Arak, H., Karimi Torshizi, M.A. and Rahimi, Sh. (2013). Study on the effect of Savory (Satureja khuzestanica) essential oil and Polysorb toxin-binder against experimental aflatoxicosis in Japanese quail. Veterinary Clinical Pathology, 7(27): 249-260. [In Persian]
Atakisi, O., Atakisi, E. and Kart, A. (2009). Effects of dietary zinc and L-arginine supplementation on total antioxidants capacity, lipid peroxidation, nitric oxide, egg weight and blood biochemical values in Japanese quails. Biological Trace Element Research, 132(1-3): 136-143.
Azad, M.A.K., Kikusato, T., Maekawa, H., Shirakawa, M. and Toyomizu, M. (2010). Metabolic characteristics and oxidative damage to skeletal muscle in broiler chickens exposed to chronic heat stress. Comparative Biochemistry and Physiology, 155(3): 401-406.
Boulton, K., Nolan, M. J., Harman, K., Psifidi, A., Wu, Z. and Bishop, S., (2017). Resistance and Tolerance are separabletraits in the innate immune response of chickens to Eimeria tenella induced coccidiosis. Veterinary Clinical Pathology, 44 (2): 28-35.
Bun, S.D., Guo, Y.M., Guo, F.C., Ji, F.J. and Cao, H. (2011). Influence of organic zinc supplementation on the antioxidant status and immune responses of broilers challenged with Eimeria tenella. Poultry Science, 90(6): 1220-1226.
Chandra, J., Samali, A. and Orrenius, S. (2000). Triggering and modulation of apoptosis by oxidative stress. Free Radical Biology and Medicine, 29(3-4): 323-333.
Craven, S.E. and Williams, D.D. (1998). In vitro attachment of Salmonella typhimurium to chicken cecal mucus: effect of cations andpretreatment with Lactobacillusspp. isolated from the intestinaltracts of chickens. Journal of Food Protection, 61(3): 265-271.
Dröge, W. (2002). Free radicals in the physiological control of cell function. Physiology Review, 82(1): 47-95.
Duan, X., Li, F., Mou, S., Feng, J., Liu, P. and Xu, L. (2015). Effects of dietary L-arginine on laying performance and anti-oxidant capacity of broiler breeder hens, eggs, and offspring during the late laying period. Poultry Science, 94(12): 2938-2943.
Giannenas, I., Papadopoulos, E., Tsalie, E., Triantafillou, E., Henikl, S., Teichmann, K. and Tontis, D. (2012). Assessment of dietary supplementation with probiotics on performance, intestinal morphology and microflora of chickens infected with Eimeria tenella. Veterinary Parasitology, 188(1-2): 31-40.
Graat, E.A., Ploeger, H.W., Henken, A.M., De Vries Reilingh, G., Noordhuzien, J.P. and Van Beek, P.N. (1996). Effect of initial litter contamination level with Eimeria acervulina on population dynamics and production characteristics in broilers. Veterinary Parasitolgy, 65(3-4): 223-232.
He, J., Hwang, G., Liu, Y., Gao, L., Kilpatrick-Liverman, L., Santarpia, P., et al. (2016). L-Arginine modifies the exopolysaccharide matrix and thwarts Streptococcus mutans outgrowth within mixed-species oral biofilms. Journal of Bacteriology, 198(19): 2651-2661.
Langrová, I., Chodová, D., Tůmová, T., Horáková, B., Krejčířová, R., Šašková, M., et al. (2019). Assessment of low doses of Eimeria tenella sporulated oocysts on the biochemical parameters and intestinal microflora of chickens. Turkish Journal of Veterinary and Animal Sciences, 43(1): 76-81.
Liopis, M., Antolin, M., Guarner, F., Salas, A. and Malagelada, J.R. (2005). Mucosal colonisation with Lactobacillus casei mitigates barrier injury induced by exposure to trinitronbenzene sulphonic acid. Gut, 54(7): 955-959.
Ma, X.Y.Y.C., Lin, Z.Y., Jiang, C.T., Zheng, G.L., Zhou, D.Q., Yu, T., et al. (2010). Dietary arginine supplementation enhances anti-oxidative capacity and improves meat quality of finishing pigs. Amino Acids, 38(1): 95-102.
Mahdavi, S. and Nobakht, A. (2018). Evaluation of the effect of Thyme (Thymus vulgaris L.) and Ziziphora (Ziziphora tenuior L.) essential oils on intestinal microflora of broilers. Veterinary Clinical Pathology, 11(44): 305-312. [In Persian]
Milinkovik-Thur, S., Stogective, Z., Prisljin, J., Zdelar-Tuk, M., Poljicak-Milas, N., Ljubic, B.B., et al. (2007). Effect of refeeding on the antioxidant system in cockerels and pullets. Acta Veterinaria Hungarica, 55(2): 181-188.
Montagne, L., Pluske, J.R. and Hampson, D.J. (2003). A review of interactions between dietary fiber and the intestinal mucosa, and their consequences on digestive health in young non-ruminant animals. Animal Feed Science and Technology, 108(1-4): 95-117.
Mookiah, S., Sieo, C.C., Ramasamy, K., Abdullah, N. and Ho, Y.W. (2014). Effects of dietary prebiotics, probiotic and synbiotics on performance, caecal bacterial populations and caecal fermentation concentrations of broiler chickens. Journal of Science Food Agriculture,94(2): 341-348.
Olfati, A., Mojtahedin, A., Sadeghi, T. Akbari, M. and Martínez-Pastor, F. (2018). Comparison of growth performance and immune responses of broiler chicks reared under heat stress, cold stress and thermoneutral conditions. Spanish Journal of Agriculture Research, 16(2): 1-7.
Perez, P.F., Minnaard, J., Rouvet, M., Knabenhans, C., Brassart, D., De Antoni, G.L., et al. (2001). Inhibition of Giardia intestinalis by extracellular factors from lactobacilli: an in vitro study. Applied Environment Microbiology, 67(11): 5037-5042.
Qin, Z.R., Fukata, T., Baba, E. and Arakawa, A. (1995). Effect of Eimeria tenella infection on Salmonella enteritidis infection in chickens. Poultry Science, 74(1): 1-7.
Rasoulifard, M.H. and Zargari, F. (2015). The effects of aqueous extract of white tea on serum antioxidant enzymes in rats exposed to arsenic. Veterinary Clinical Pathology, 9(34): 153-178. [In Persian]
Ren, W., Chen, S., Yin, J., Duan, J., Li, T., Liu, G., et al. (2014). Dietary arginine supplementation of mice alters the microbial population and activates intestinal innate immunity. The Journal of Nutrition, 144(6): 988-995.
Ren, W., Zou, L., Li, N., Wang, Y., Liu, G., Peng, Y., et al. (2013). Dietary arginine supplementation enhances immune responses to inactivated Pasteurella multocida vaccination in mice. British Journal of Nutrition, 109(5): 867-72.
Sergeant, M.J., Constantinidou, C., Cogan, T.A., Bedford, M.R., Penn, C.W. and Pallen, M.J. (2014). Extensive Microbial and Functional Diversity within the Chicken Cecal Microbiome, Plos One, 9(3): 18-21.
Tierney, J., Gowing, H., Van Sinderen, D., Flynn, S., Stanley, L., McHardy, N., et al. (2004). In vitro inhibition of Eimeria tenella invasion by indigenous chicken Lactobacillus species. Veterinary Parasitology, 122(3): 171-182.
Williams, R.B. (1999). A compartmentalised model for the estimation of the cost of coccidiosis to the world’s chicken production industry. International Journal of Parasitology, 29(8): 1209-1229.
Wu, G. and Meininger, C.J. (2000). Arginine nutrition and cardiovascular function. Journal of Nutrition, 130(11): 2626-2629.
Wu, Z.G., Hu, T.J., Rothwell, L., Vervelde, L., Kaiser, P., Boulton, K., et al. (2016). Analysis of the function of IL-10 in chickens using specific neutralising antibodies and a sensitive capture ELISA. Developmental and Comparative Immunology, 63(1): 206-212.