مطالعه اثر عصاره فلفل کوهی (Vitagnus castus) بر بلوغ و لقاح آزمایشگاهی اووسیت موش‌های سوری مبتلاشده به سندروم تخمدان پلی‌کیستیک
الموضوعات :
توحید قربانی
1
,
امیر کریمی
2
,
غلامرضا نجفی
3
,
مقصود بشارتی
4
,
محسن شرفی
5
1 - دانشجوی فیزیولوژی دام، دانشکده کشاورزی و منابع طبیعی اهر، دانشگاه تبریز، تبریز، ایران.
2 - استادیار گروه علوم دامی دانشکده کشاورزی و منابع طبیعی اهر، دانشگاه تبریز، تبریز، ایران.
3 - دانشیار گروه علوم آناتومی، دانشکده دامپزشکی، دانشگاه ارومیه، ارومیه، ایران.
4 - استادیار گروه علوم دامی دانشکده کشاورزی و منابع طبیعی اهر، دانشگاه تبریز، تبریز، ایران.
5 - استادیار گروه علوم طیور، دانشکدة کشاورزی، دانشگاه تربیت مدرس، تهران، ایران.
تاريخ الإرسال : 12 السبت , ربيع الأول, 1441
تاريخ التأكيد : 17 السبت , محرم, 1442
تاريخ الإصدار : 03 السبت , محرم, 1442
الکلمات المفتاحية:
لقاح آزمایشگاهی,
بلوغ آزمایشگاهی,
سندروم تخمدان پلی‌کیستیک,
فلفل‌کوهی,
ملخص المقالة :
عدم کاهش کلاژن دیواره فولیکولی منجر به تجمع فولیکول ها و بروز سندرم تخمدان پلی کیستیک و کاهش کیفیت اووسیت ها می‌گردد. هدف ازمطالعه حاضر بررسی اثر عصاره گیاه فلفل کوهی (Vitagnus castus) بر بلوغ و توان باروری آزمایشگاهی اووسیت‌ها در موش های سوری مبتلاشده به سندرم تخمدان پلی کیستیک (ovarian syndrome; PCOpolycystic) بود. بدین منظور تعداد 32 سر موش سوری ماده NMRI (Naval Medical Research Institute) ماده نابالغ 25 روزه با میانگین وزنی 25 گرم تهیه شده از دانشگاه تبریز، به طور تصادفیبه 4 گروه آزمایشی به شرح زیر تقسیم شدند: گروه شاهد بدون دریافت عصاره، گروه کنترل PCO بدون دریافت عصاره، گروه PCO دریافت کننده365 میلی گرم عصاره فلفل کوهی و گروه PCO دریافت کننده730میلی گرم عصاره فلفل‌کوهی. برای تهیه اووسیت، موش های آزمایشی توسط تزریق درون صفاتی هورمون PMSG (pregnant mare serum gonadotropin) تیمار شدند و بعد از آسان کشی،از فولیکول های تخمدانی تخمک گیری به عمل آمده و برای ارزیابی بلوغ و لقاح آزمایشگاهی مورد استفاده قرار گرفتند.نتایج نشان داد که مقادیر هورمون های تستوسترون و استروژن در گروه های PCO تیمارشده با عصاره فلفل کوهی نسبت به گروه PCOدریافت کننده استرادیول والرات به طور معنی داری پایین تر بود (05/0>p < /em>).همچنین تعداد اووسیت های نابالغی که به مرحله متافازII رسیدند در گروهPCOدریافت کننده730 میلی گرم عصاره فلفل کوهی نسبت به گروه PCO دریافت کننده استرادیول والرات به طور معنی داری بالاتربود (05/0>p < /em>). اما درصد اووسیت های لقاح یافته و نیز بلاستوسیست های تولیدی بین گروه های آزمایشی تفاوت معنی داری با هم نداشتند (05/0<p < /em>). از طرف دیگر درصد رویان های هچ شده در گروه PCO دریافت کننده365 میلی گرم عصاره فلفل کوهی به طور معنی داری بالاتر از گروه PCOدریافت کننده استرادیول والرات بود (05/0>p < /em>). یافته های پژوهش حاضر نشان داد که عصاره گیاه فلفل کوهی باعث تکامل بیشتر اووسیت ها جهت لقاح و نیز افزایش تکامل رویان های حاصل از لقاح آزمایشگاهی در حیوانات درگیربا سندرم تخمدان پلی کیستیک می شود.
المصادر:
Altonen, J., Laitinen, M.P., Voujolainen, K., Jaatinen, R., Horelli-Kuitunen, N. and Seppä, L. (1999). Human growth differentiation factor 9 (GDF-9) and its novel homolog GDF-9B are expressed in oocytes during early folliculogenesis. The Journal of Clinical Endocrinology and Metabolism, 84(8): 2744-2750.
Borrione, P., Di Luigi, L., Maffulli, N. and Pigozzi, F. (2008). Herbal supplements: cause for concern? Journal of Sport Science and Medicine, 7(4): 562-564.
Brock, B., Smidt, K., Ovesen, P., Schmitz, O. and Rungby, J. (2005). Is metformin therapy for Polycystic Ovary Syndrome safe during pregnancy? Basic & Clinical Pharmacology and Toxicology, 96(6): 410-412.
Daghigh Kia, H., Sadeghi Sadegh Abad, F., Ebrahimi, M. and Samadian, F. (2017). Comparative effect of different concentrations of hydro-ethanolic extract of chamomile on freeze-thawn semen quality of rams. Veterinary Clinical Pathology, 11(41): 13-23. [In Persian]
Daniele, C., Thopson, C.J., Pittler, M.H. and Ernest, E. (2005). Vitex agnus-castus a systemathic review of adverse event. Drug Safety, 28(4): 319-322.
Dumesic, D.A., Meldrum, D.R., Katz-Jaffe, M.G., Krisher, R.L. and Schoolcraft, W.B. (2015). Oocyte environment: follicular fluid and cumulus cells are critical for oocyte health. Fertility and Sterility, 103(2): 303-316.
Dumesic, D.A, Padmanabhan, V. and Abbott, D.H. (2008). Polycystic ovary syndrome and oocyte developmental competence. Obstetrics and Gynecology, 63(1): 39-48.
Franks, S. (2009). Do Animal Models of Polycystic Ovary Syndrome Help to Understand Its Pathogenesis and Management? Yes, but Their Limitations should be Recognized. Endocrinology, 150(9): 3983-3985.
Gonzalez, F., Thusu, K., Abdel-Rahman, E., Prabhala, A., Tomani, M. and Dandona, P. (1999). Elevated serum levels of tumor necrosis factor alpha in normal-weight women with polycystic ovary syndrome. Metabolism, 48(4): 437-441.
Henmi, H., Endo, T., Nagasawa, K., Hayashi, T., Chida, M. and Akutagawa, N. (2001). Lysyl oxidase and MMP-2 expression in dehydroepiandrosterone induced polycystic ovary in rats. Biology of Reproduction, 64(1): 157-162.
Hossain Rashidi, B. and Nemati, M. (2017). Effects of Vitex agnus-castus extract on the secretory function of pituitary-gonadal axis and pregnancy rate in patients with premature ovarian aging (POA). Journal of Herbal Medicine, 10: 24-30.
Handa, R.J., Pak, T.R., Kudwa, A.E., Lund, T.D. and Hinds, L. (2008) An alternate pathway for androgen regulation of brain function: activation of estrogen receptor beta by the metabolite of dihydrotestosterone, 5alpha-androstane-3beta,17 beta-diol. Hormones and Behavior, 53(5): 741-752.
Jelodar, G.A. and Askari, K. (2012). Effect of Vitex agnus castus fruits hydroalcoholic extract on sex hormones in rat with induced polycystic ovary syndrome (PCOS). Journal of Physiology and Pharmacology, 16(1): 62-69. [In Persian]
Jelodar, G.A. and Askari, K. (2017). Effect of hydroalcoholic extract of Vitex agnus-castus fruit on fertility and estrous cycle in letrozole-induced polycystic ovary syndrome in rat. Razi Journal of Medical Sciences, 24(156): 42-48. [In Persian]
Marx, T.L. and Mehta, A.E. (2003). Polycystic ovary syndrome: pathogenesis and treatment over the short and long term. Cleveland Clinic Journal of Medicine, 70(1): 31-45.
Mohammadzadeh, H., Delashoub, M. and Khakpour, M. (2018). Effect of vitamin E in prevention of lipopolysaccharide induced fetal injuries in the rat. Veterinary Clinical Pathology, 11(44): 367-377. [In Persian]
Mostahsan, Z. and Mortazavi, P. (2019). The effects of Tilia (Tilia plathyphyllos) extract on gene expression of caspase3 and caspase9 in canine mammary gland cancer cell line (CF41.Mg). Veterinary Clinical Pathology, 13(49): 2-14. [In Persian]
Nabiuni, M., Mohammadi, S., Kayedpoor, P. and Karimzadeh, L., (2015). The effect of curcumin on the estradiol valerate-induced polycystic ovary in rats. Kashan University of Medical Sciences Journal (FEYZ), 18(6): 515-523. [In Persian]
Nasri, S., Oryan, S.h., HaeriRohani, A., Amin, G.H. and Taghizadeh, M. (2005). The effects of Vitexagnuscastus L. extract and interaction with bromocriptine on luteinizing hormone and testosterone in male mice. Medical Journal of Hormozgan University, 9(2): 113-118. [In Persian]
Noorafshan, A., Ahmadi, M., Mesbah, S. and Karbalay-Doust, S. (2013). Stereological study of the effect of letrozole and estradiol valerate treatment on the ovary of rats. Clinical and Experimental Reproductive Medicine, 40(3): 115-121.
Palep-Singh, M., Picton, H.M., Yates, Z.R., Barth, J.H. and Balen, A.H. (2008). Plasma homocysteine concentrations and the single nucleotide polymorphisms in the methionine synthase gene (MTR 2756A>G): Associations with the polycystic ovary syndrome an observational study. The European Journal of Obstetrics & Gynecology and Reproductive Biology, 138(2): 180-186.
Rani, A. and Sharma, A. (2013). The genus Vitex: A review. Pharmacognosy Reviews, 7(14): 188–198.
Robinson, J.E., Forsdike, R.A. and Taylor, J.A. (1999). In utero exposure of female lambs to testosterone reduces the sensitivity of the GnRH neuronal network to inhibition by progesterone. Endocrinology, 140(12): 5797-5805.
Shafiee, M.N., Malik, D.A., Yunos, R.I.M., Atiomo, W., Omar, M.H., Ghani, N.A.A. et al. (2015). The effect of Metformin on endometrial tumor-regulatory genes and systemic metabolic parameters in polycystic ovarian syndrome–a proof-of-concept study. Gynecological Endocrinology, 31(4): 286-290.
Shamsi, M., Nejati, V. and Najafi, G. (2015). Therapeutic Effects of Licorice Extract on In vitro Maturation and In vitro Fertilization in Mice Model of Polycystic Ovary Syndrome. Journal of Mazandaran University of Medical Science, 25(132): 113-121. [In Persian]
Stadtmauer, L.A., Toma, S.K., Riehl, R.M. and Talbert, L.M. (2001). Metformin treatment of patients with polycystic ovary syndrome undergoing in vitro fertilization improves outcomes and is associated with modulation of the insulin-like growth factor. Fertility and Sterility, 75(3): 505-509.
Tsilchorozidou, T., Overton, C. and Conway, G.S. (2004). The pathophysiology of polycystic ovary syndrome. Clinical Endocrinology, 60(1): 1-17.
van Houten, E.L. and Visser, J.A. (2014). Mouse models to study polycystic ovary syndrome: a possible link between metabolism and ovarian function? Reproductive Biology, 14(1): 32-43.
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Altonen, J., Laitinen, M.P., Voujolainen, K., Jaatinen, R., Horelli-Kuitunen, N. and Seppä, L. (1999). Human growth differentiation factor 9 (GDF-9) and its novel homolog GDF-9B are expressed in oocytes during early folliculogenesis. The Journal of Clinical Endocrinology and Metabolism, 84(8): 2744-2750.
Borrione, P., Di Luigi, L., Maffulli, N. and Pigozzi, F. (2008). Herbal supplements: cause for concern? Journal of Sport Science and Medicine, 7(4): 562-564.
Brock, B., Smidt, K., Ovesen, P., Schmitz, O. and Rungby, J. (2005). Is metformin therapy for Polycystic Ovary Syndrome safe during pregnancy? Basic & Clinical Pharmacology and Toxicology, 96(6): 410-412.
Daghigh Kia, H., Sadeghi Sadegh Abad, F., Ebrahimi, M. and Samadian, F. (2017). Comparative effect of different concentrations of hydro-ethanolic extract of chamomile on freeze-thawn semen quality of rams. Veterinary Clinical Pathology, 11(41): 13-23. [In Persian]
Daniele, C., Thopson, C.J., Pittler, M.H. and Ernest, E. (2005). Vitex agnus-castus a systemathic review of adverse event. Drug Safety, 28(4): 319-322.
Dumesic, D.A., Meldrum, D.R., Katz-Jaffe, M.G., Krisher, R.L. and Schoolcraft, W.B. (2015). Oocyte environment: follicular fluid and cumulus cells are critical for oocyte health. Fertility and Sterility, 103(2): 303-316.
Dumesic, D.A, Padmanabhan, V. and Abbott, D.H. (2008). Polycystic ovary syndrome and oocyte developmental competence. Obstetrics and Gynecology, 63(1): 39-48.
Franks, S. (2009). Do Animal Models of Polycystic Ovary Syndrome Help to Understand Its Pathogenesis and Management? Yes, but Their Limitations should be Recognized. Endocrinology, 150(9): 3983-3985.
Gonzalez, F., Thusu, K., Abdel-Rahman, E., Prabhala, A., Tomani, M. and Dandona, P. (1999). Elevated serum levels of tumor necrosis factor alpha in normal-weight women with polycystic ovary syndrome. Metabolism, 48(4): 437-441.
Henmi, H., Endo, T., Nagasawa, K., Hayashi, T., Chida, M. and Akutagawa, N. (2001). Lysyl oxidase and MMP-2 expression in dehydroepiandrosterone induced polycystic ovary in rats. Biology of Reproduction, 64(1): 157-162.
Hossain Rashidi, B. and Nemati, M. (2017). Effects of Vitex agnus-castus extract on the secretory function of pituitary-gonadal axis and pregnancy rate in patients with premature ovarian aging (POA). Journal of Herbal Medicine, 10: 24-30.
Handa, R.J., Pak, T.R., Kudwa, A.E., Lund, T.D. and Hinds, L. (2008) An alternate pathway for androgen regulation of brain function: activation of estrogen receptor beta by the metabolite of dihydrotestosterone, 5alpha-androstane-3beta,17 beta-diol. Hormones and Behavior, 53(5): 741-752.
Jelodar, G.A. and Askari, K. (2012). Effect of Vitex agnus castus fruits hydroalcoholic extract on sex hormones in rat with induced polycystic ovary syndrome (PCOS). Journal of Physiology and Pharmacology, 16(1): 62-69. [In Persian]
Jelodar, G.A. and Askari, K. (2017). Effect of hydroalcoholic extract of Vitex agnus-castus fruit on fertility and estrous cycle in letrozole-induced polycystic ovary syndrome in rat. Razi Journal of Medical Sciences, 24(156): 42-48. [In Persian]
Marx, T.L. and Mehta, A.E. (2003). Polycystic ovary syndrome: pathogenesis and treatment over the short and long term. Cleveland Clinic Journal of Medicine, 70(1): 31-45.
Mohammadzadeh, H., Delashoub, M. and Khakpour, M. (2018). Effect of vitamin E in prevention of lipopolysaccharide induced fetal injuries in the rat. Veterinary Clinical Pathology, 11(44): 367-377. [In Persian]
Mostahsan, Z. and Mortazavi, P. (2019). The effects of Tilia (Tilia plathyphyllos) extract on gene expression of caspase3 and caspase9 in canine mammary gland cancer cell line (CF41.Mg). Veterinary Clinical Pathology, 13(49): 2-14. [In Persian]
Nabiuni, M., Mohammadi, S., Kayedpoor, P. and Karimzadeh, L., (2015). The effect of curcumin on the estradiol valerate-induced polycystic ovary in rats. Kashan University of Medical Sciences Journal (FEYZ), 18(6): 515-523. [In Persian]
Nasri, S., Oryan, S.h., HaeriRohani, A., Amin, G.H. and Taghizadeh, M. (2005). The effects of Vitexagnuscastus L. extract and interaction with bromocriptine on luteinizing hormone and testosterone in male mice. Medical Journal of Hormozgan University, 9(2): 113-118. [In Persian]
Noorafshan, A., Ahmadi, M., Mesbah, S. and Karbalay-Doust, S. (2013). Stereological study of the effect of letrozole and estradiol valerate treatment on the ovary of rats. Clinical and Experimental Reproductive Medicine, 40(3): 115-121.
Palep-Singh, M., Picton, H.M., Yates, Z.R., Barth, J.H. and Balen, A.H. (2008). Plasma homocysteine concentrations and the single nucleotide polymorphisms in the methionine synthase gene (MTR 2756A>G): Associations with the polycystic ovary syndrome an observational study. The European Journal of Obstetrics & Gynecology and Reproductive Biology, 138(2): 180-186.
Rani, A. and Sharma, A. (2013). The genus Vitex: A review. Pharmacognosy Reviews, 7(14): 188–198.
Robinson, J.E., Forsdike, R.A. and Taylor, J.A. (1999). In utero exposure of female lambs to testosterone reduces the sensitivity of the GnRH neuronal network to inhibition by progesterone. Endocrinology, 140(12): 5797-5805.
Shafiee, M.N., Malik, D.A., Yunos, R.I.M., Atiomo, W., Omar, M.H., Ghani, N.A.A. et al. (2015). The effect of Metformin on endometrial tumor-regulatory genes and systemic metabolic parameters in polycystic ovarian syndrome–a proof-of-concept study. Gynecological Endocrinology, 31(4): 286-290.
Shamsi, M., Nejati, V. and Najafi, G. (2015). Therapeutic Effects of Licorice Extract on In vitro Maturation and In vitro Fertilization in Mice Model of Polycystic Ovary Syndrome. Journal of Mazandaran University of Medical Science, 25(132): 113-121. [In Persian]
Stadtmauer, L.A., Toma, S.K., Riehl, R.M. and Talbert, L.M. (2001). Metformin treatment of patients with polycystic ovary syndrome undergoing in vitro fertilization improves outcomes and is associated with modulation of the insulin-like growth factor. Fertility and Sterility, 75(3): 505-509.
Tsilchorozidou, T., Overton, C. and Conway, G.S. (2004). The pathophysiology of polycystic ovary syndrome. Clinical Endocrinology, 60(1): 1-17.
van Houten, E.L. and Visser, J.A. (2014). Mouse models to study polycystic ovary syndrome: a possible link between metabolism and ovarian function? Reproductive Biology, 14(1): 32-43.
