• الصفحة الرئيسية
  • بررسی فیتوشیمیایی، ارزیابی عملکرد آنتی‌اکسیدانی و آنتی-باکتریایی عصاره‌های پنج گونه دارویی Pulicaria gnaphalodes (Vent.) Boiss، Rhazya stricta L.، Salvadora persica L.، Teucrium polium L.، Hibiscus sabdariffa L. در استان سیستان و بلوچستان

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عنوان URL للمقالة


رقم المقالة : EJMP-1702-1192 (R1) زيارة : 156 الصفحة: 49 - 68

20.1001.1.23223235.1396.5.2.5.6

نوع المخطوط: ابحاث

بررسی فیتوشیمیایی، ارزیابی عملکرد آنتی‌اکسیدانی و آنتی-باکتریایی عصاره‌های پنج گونه دارویی Pulicaria gnaphalodes (Vent.) Boiss، Rhazya stricta L.، Salvadora persica L.، Teucrium polium L.، Hibiscus sabdariffa L. در استان سیستان و بلوچستان

الموضوعات :

امید عزیزیان شرمه 1 (دانش‌آموخته کارشناسی ارشد فیتوشیمی، باشگاه پژوهشگران جوان و نخبگان، واحد زاهدان، دانشگاه آزاد اسلامی، زاهدان، ایران)
محرم ولی زاده 2 (استادیار، گروه تولیدات گیاهی، دانشکده کشاورزی، مجتمع آموزش عالی سراوان، سراوان، ایران)
علی قاسمی 3 (مربی، گروه زیست شناسی، دانشکده علوم پایه، دانشگاه سیستان و بلوچستان، زاهدان، ایران)
احمد مهربان 4 (استادیار، گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه آزاد اسلامی واحد زاهدان، زاهدان، ایران)
افسانه کمالی دلجو 5 (دانش آموخته کارشناسی ارشد زراعت و اصلاح نباتات، باشگاه پژوهشگران جوان و نخبگان، واحد زاهدان، دانشگاه آزاد اسلامی، زاهدان، ایران)

تاريخ الإرسال : 06 الجمعة , جمادى الأولى, 1438 تاريخ التأكيد : 11 السبت , رجب, 1438 تاريخ الإصدار : 01 الأربعاء , ذو الحجة, 1438

الکلمات المفتاحية: سیستان و بلوچستان, آنتی‌اکسیدانی, فلاونوئید, فنول, آنتی‌باکتریایی, گیاهان دارویی بومی,

ملخص المقالة :

مطالعه حاضر به بررسی ترکیبات فیتوشیمیایی فنول و فلاونوئید کل، ارزیابی عملکرد آنتی اکسیدانی و آنتی باکتریایی سه عصاره متانولی، اتانولی و آبی پنج گونه مختلف گیاهان دارویی Pulicaria gnaphalodes (Vent.) Boiss.، Rhazya stricta L.، Salvadora persica L.، Teucrium polium L. و Hibiscus sabdariffa L. در منطقه سیستان و بلوچستان می پردازد. پس از جمع آوری گیاهان و تهیه عصاره ها به روش ماسراسیون، محتوای فنولی و فلاونوئیدی به‌ترتیب با روش های معرف فولین-سیوکالتیو و رنگ سنجی کلرید آلومینیوم، فعالیت آنتی اکسیدانی با دو روش DPPH و FRAP و فعالیت آنتی باکتریایی علیه باکتری های استافیلوکوکوس اورئوس، انتروکوکوس فکالیس، اشرشیاکلی و سالمونلا تیفیموریوم به روش دیسک دیفیوژن (انتشار دیسک) سنجیده شده است. نتایج نشان دادند که در تمامی گونه ها، عصاره متانولی دارای بیشترین ترکیبات ثانویه و فعالیت های آنتی‌اکسیدانی و آنتی باکتریایی بوده است. عصاره متانولی گونه Hibiscus sabdariffa L. دارای بیشترین میزان ترکیبات فنولی (mgGAE/g44/6±54/32) و فلاونوئیدی (mgGAE/g00/1±11/27) و فعالیت آنتی اکسیدانی (µg/ml21/1±34/17IC50= و mMFe2+/mg27/2±19/55) و فعالیت آنتی باکتریایی علیه باکتری استافیلوکوکوس اورئوس با قطر‌هاله عدم رشد 00/1±27 میلی متر بوده است. در مقابل عصاره آبی گونه Teucrium poliumL.دارای کمترین میزان ترکیبات فنولی (mgGAE/g11/1±33/4) و فلاونوئیدی (mgGAE/g08/0±03/2) و فعالیت آنتی‌اکسیدانی (µg/ml02/2±71/220 IC50= و mM Fe2+/mg sample99/0±76/8) و فعالیت آنتی باکتریایی علیه باکتری انترکوکوس فکالیس با قطر‌هاله عدم رشد 50/1±4 میلی متر و اشرشیاکلی با قطر‌ هاله عدم رشد 57/0±4 میلی متر بوده است. به‌طورکلی بر اساس نتایج بدست آمده، گیاهان مورد مطالعه می توانند کاندیدای خوبی جهت درمان بیماری های ناشی از استرس‌های اکسیداتیوی و بیماری های ناشی از میکروب های بیماری زا قرار گیرند.

المصادر:


  1. Abier, H.S., Claesson, R.L.K., Lingstrom, P K.and Gustafsson, A K. 2008. Strong antibacterial effect of Miswak against oral microorganisms associated with periodontitis and caries. Journal of Periodontology, 79(8): 1474-1479.
    2.
  2. Ajaib, M., Mati-ur-Rehman, A., Mohammed Khan, Kh., Perveen, Sh. and Shah, Sh. 2015.  Pulicaria undulata: A potential phytochemical, antimicrobial and antioxidant source. Journal of the Chemmical Society of Pakistan, 37(3); 559-566.
    3.
  3. Akhtar, AK., Haq, T.U. and Mirza, B. 2015. Phytochemical analysis and comprehensive evaluation of antimicrobial and antioxidant properties of 61 medicinal plant species. Arabian Journal of Chemistry, 1(2): 1-13.
    4.
  4. Alali, F., Hudaib, M., Aburjai, T., Khairallah, K. and Al-Hadidi, N. 2005. GC-MS analysis and antimicrobial activity of the essential oil from the stem of the jordanian tooth brush tree Salvadora persica. Pharmaceutical Biology, 42(8): 577-80.
    5.
  5. Al-Bagieh, N.H., Idow, A. and Salako, N.O. 1994. Effect of aqueous extract of Miswak on the in vitro growth of Candida albicans. Microbiolology Letters, 80: 107–113.
    6.
  6. Al-Hajj, N.Q.M., Wang, H., Gasmalla, M.A.A., Chaoyang, M., Thabit, R., Rahman, M.D.R.T. and Tang, Y. 2014. Chemical composition and antioxidant activity of the essential oil of Pulicaria Inuloides. Journal of Food and Nutrition Research, 2(5): 221-227.
    7.
  7. Al-Hajj, N.Q.M., Wang, H.X., ChaoyangM M., Lou, Z., Bashari, M. and Thabit, E. 2014. Antimicrobial and antioxidant activities of the essentialoils of some aromatic medicinal plants (Pulicaria inuloides-Asteraceae and Ocimum forskolei-Lamiaceae). Tropical Journal of Pharmaceutical Research August, 13 (8): 1287-1293.
    8.
  8. Al-Hashimi, A.G. 2012. Antioxidant and antibacterial activities of Hibiscus sabdariffa L. extracts. African Journal of Food Science, 6(21): 506-511.
    9.
  9. Ali, B.H., Al-Qarawi, A.A., Bashir, A.K. and Tanira, M.O. 2000. Antioxidant action of extract of the traditional medicinal plant Rhazya stricta Decne in rats. Phototherapy Research. 14: 469–471.
    10.
  10. Al-sieni, AI. 2013. The antibacterial activity of traditionally used Salvadora persica L. (miswak) and Commiphora gileadensis (palsam) in Saudi Arabia. African Journal of Traditional, Complementary and Alternative medicines, 11(1): 23-27.
    11.
  11. Asghari, JH., Gorganli Doji, T. and Ghaemi, A. 2014. Phytochemical and antimicrobial investigation of essencial oil of Ferula gummosa Boiss. in meighan region  Semnan province. Eco-phytochemical Journal of Medicinal Plants, 1(5): 28-35. (In Persian)
    12.
  12. Azizian Shermeh, O., Valizadeh, J., Noroozifar, M., and Qasemi, A. 2016. Investigation of antibacterial activities of silver nanoparticles by aqueous extract of Sambacus ebulus L. Journal of Ilam University of Medical Sciences, 25(4):92-108. (In Persian)
    13.
  13. Babayi, H., Kolo, I., Okogun, I. and Ijah, J. 2004. The antimicrobial activities of methanolic extracts of Eucalyptus camaldulensis and Terminalia catappa against some pathogenic microorganisms. Biokemistri, 6: 106-111.
    14.
  14. Balouiri, M., Sadiki, M. and Koraichi Ibnsouda, S. 2016. Methods for in vitro evaluating antimicrobial activity: A review. Journal of Pharmaceutical Analysis, 6: 71–79.
    15.
  15. Bauer, A.W., Kirby, W.M., Sherris, J.C. andTurck, M. 1966. Antibiotic susceptibility testing by a standardized single disk method. American Journal of Clinical Pathology,45, 493–496.
    16.
  16. Bergmeier, D., Berres, P.H.D., Filippi, D., Bilibio, D., Bettiol, V.R. and Priamo, W.L. 2014. Extraction of total polyphenols from Hibiscus (Hibiscus sabdariffa L.) and wax weed/‘sete-sangrias’(Cuphea carthagenensis) and evaluation of their antioxidant potential. Acta Scientiarum Technology, 36: 545-51.
    17.
  17. Borneo, R., Leon, E.A., Aguirre, A., Ribotta, P. and Cantero, J.J. 2008. Antioxidant capacity of          medicinal plants from the Province of Cordoba (Argentina) and their in vitro testing in model food system. Food Chemistry, 112: 664-670.
    18.
  18. Chang, C., Yang, M., Wen, H. and Chern, J. 2002. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. Journal of Food and Drug Analysis, 10: 178-182.
    19.
  19. Da-Costa-Rocha, I., Bonnlaender, B., Sievers, H., Pischel, I. and Heinrich, M. 2014. Hibiscus sabdariffa L. – A phytochemical and pharmacological review. Food Chemistry. 165: 424–443.
    20.
  20. Dridi, A., Hadef, Y. and Bouloudani, L.  2016. Determination of stotal Phenol, Flavonoid, antioxidant and antimicrobial activity of methanolic extract of Teucrium polium L. in Algerian East. International Journal of Pharmacognosy and Phytochemical Research, 8(10); 1566-1570.
    21.
  21. Ebrahimzadeh, MA, Pourmorad, F., and Hafezi, S. 2008. Antioxidant activities of Iranian corn silk. Turkish Journal of Biology, 32: 43-49.
    22.
  22. EL-Hefny, M., Hayssam, M.A.,  Ashmawy, N.A. and Salem, M.Z.M. 2017. Chemical composition and bioactivity of Salvadora persica extracts against some potato bacterial pathogens. BioResources, 12(1): 1835-1849.
    23.
  23. Garcia-Alonso, J., Ros, G., Vidal-Guevara, L. and Periago, J. 2006. Acute intake of phenolic-rich juice improves antioxidant status in healthy subjects. Nutrition Research, 26: 330-339.
    24.
  24. Gherib, M., Chahrazed, B., Abdelhamid, E.H.I., Chaouche, T.M. and Atik-Bekkara, F. 2016.  Antioxidant and antibacterial of aerial part essential oil and some organic extracts from the Algerian medicinal plant Pulicaria mauritanica coss. International Journal of Pharmaceutical Sciences and Research, 7(1): 76-84.
    25.
  25. Ghiselli, A., Serafini, M., Natella, F. and Scaccini, C. 2000. Total antioxidant capacity as a tool to assess redox status: critical review and experimental data. Free Radical Biology and Medicine, 29: 1106-1114.
    26.
  26. Guiche, R.E.L., Tahrouch, S., Amri, O., Mehrach, K.E.L. and Hatimie, A. 2015. Antioxidant activity and total phenolic and flavonoid contents of 30 medicinal and aromatic, plants located in the South of Morocco. International Journal of New Technology and Research, 1(3): 7-11.
    27.
  27. Gulcin, I., uguz, M., Oktay, M., Beydemir, S.O. and Kufreviolgu, I. 2003. Antioxidant and antimicrobial activities of Teucrium polium L. Journal of Food Technology, 1(1): 9-16.
    28.
  28. Guo, C., Yang, J., Wei, J., Li, Y., Xu, J. and Jiang, Y. 2003. Antioxidant activities of peel, pulp and seed fractions of common fruits as determined by FRAP assay. Nutrition Research, 23: 1719–1726.
    29.
  29. Gupta, A., Verma, S., Kushwaha, P., Srivastava, S., and A K S Rawat, A.K.S. 2015. Phytochemical and Antioxidant Studies of Salvadora persica L. Stem and Twig. Indian Journal of Pharmaceutical Education and Research, 49(1): 71-75.
    30.
  30. Halawany, HS. 2012. A review on miswak (Salvadora persica) and its effect on various aspects of oral health. The Saudi Dental Journal, 24: 63–69.
    31.
  31. Halliwell, B. and Gutteridge, J.M.C. 2007. Free Radicals in Biology and Medicine, 4th edition Clarendon, Oxford. 725 p.
    32.
  32. Heatley, N.G. 1944. Method for the assay of penicillin. Biochemcal Journal. 38: 61-65.
    33.
  33. Huang, D., Ou, B. and Prior, R. L. 2005. The chemistry behind antioxidant capacity assays. Journal of Agricultural and Food Chemistry, 53, 1841–1856.
    34.
  34. Jamshidi, M., Ahmadi, H.R., Rezazadeh, S., Fathi, F. and Mazanderani, M. 2010. Study on phenolic and antioxidant activity of some selected plant of Mazandaran province. Medicinal Plant; 9(34): 177-183.
    35.
  35. Javadian, F., Sepehri, Z., Amraee, M., Kiani, Z., Shahraki Mojahed, M., Shahi, Z. and Pourghasemi Fetideh, S. 2015. An evaluation of antibacterial activity of ethanolic extract of sour tea (Hibiscus Sabdariffa) against Klebsiella Pneumoniae Resistant to antibiotics, Journal of Sabzevar University of Medical Science, 22(4), 565-570.
    36.
  36. Kamkar, A., Asadi, F., Jenneli-javan, A. and Jamshidi, R. 2009. Antioxidant capacity of essential oil and extract of Iranian Mentha spicata, Journal of Veterinary Medicin and Labarotory, 1(1), 69-77.
    37.
  37. Khanizadeh, Sh., Tsao, R., Rekika, D., Yang, R., Charles, M.T. and Rupasinghe, H.P.V. 2008. Polyphenol composition and total antioxidant capacity of selected apple genotypes for processing. Journal of Food Composition and analysis, 21: 396-401.
    38.
  38. Khorasani Esmaeili, A., Taha, R. M., Mohajer, S. and Banisalam, B. 2015. Antioxidant activity and total phenolic and Flavonoid content of various solvent extracts from In vivo and In vitro grown trifolium pratense L. (Red Clover). BioMed Research International, 2015: 1-11.
    39.
  39. Kordi tamandani, E., Valizadeh, J.and Valizadeh, M. 2014. In vitro production of secondary metabolites in Cicer spiroceras using elicitors. Global Journal of Research on Medicinal Plants and Indigenous Medicine, 3(2):  48-56.
    40.
  40. Mathew, S. and Abraham, T.E. 2006. In vitro antioxidant activity and scavenging effects of Cinnamomum verum leaf extract assayed by different methodologies. Food and Chemical Toxicology, 44: 198-206.
    41.
  41. Mir, A.R. and Mirshekari, M. 2013. The study of Sistan and Baluchestan Province. Publishing company's textbooks, Tehran, 140 p.
    42.
  42. Mohamed, H., Ons, M., Yosra, E., Rayda, S., Neji, J. and Moncef, N. 2013. Chemical composition and antioxidant and radical-scavenging activities of Periploca laevigata root bark extracts. Journal of the Science of Food and Agriculture, 89(5): 897–905.
    43.
  43. Mohamed, S.A. and Khan, J.A. 2014. Antioxidant capacity of chewing stick miswak (Salvadorapersica), BMC Complementary and Alternative Medicine, 13(40): 1-6.
    44.
  44. Mohajerani, M. 2012. Antioxidant Activity and Total Phenolic Content of Nerium oleander L. Grown in North of Iran. Iranian Journal of Pharmaceutical Research, 11(4): 1121-1126.
    45.
  45. Moure, A., Cruz, J. M., Franco, D., Dominguez, J.M., Sineiro, J., Dominguez, H., Nunez, M.J. and Parajo, J.C. 2001. Natural antioxidants from residual sources. Food Chemistry. 72(2):145-171.
    46.
  46. Muller, L., Frohlich, K. and Bohm, V. 2011. Comparative antioxidant activities of carotenoids measured by ferric reducing antioxidant power (FRAP), ABTS bleaching assay (aTEAC), DPPH assay and peroxyl radical scavenging assay. Food Chemistry, 129: 139–148.
    47.
  47. Najat, A.B., Al-Otaibi, R.A. and Ibhrahim, M.M. 2015. Phytochemical and taxonomic evaluation of Rhazyastricta in Saudi Arabia, Saudi Journal of Biological Sciences, 1(1):1-9.
    48.
  48. Nanasombat, S. and Lohasupthawee, P. 2005. Antibacterial activity of crude ethanolic extracts and essential oils of spices against Salmonellae and other enterobacteria. KMITL Science and Technology Journal,  5:  527-538.
    49.
  49. Norhaizan, M.E., Tong, S.H., Amin, I. and Chew, L.Y. 2010. Antioxidant activity in different parts of the Roselle (Hibiscus sabdariffa L) extracts and potential exploitation of the seeds. Food Chemistry, 122: 1055-1060.
    50.
  50. Noumi, E., Snoussi, M., Hajlaoui, H., Valentin, E.and Bakhrouf, A. 2010. Antifungal properties of Salvadora persica and Juglans regia L. extracts against oral Candida strains. European Journal of Clinical Microbiology and Infectious Diseases, 29(1): 81-8.
    51.
  51. Nouri, S., Kiasat, A.R., Kolahi. M., Mirzajani, R. and Seyednejad, S.M. 2016. Phytochemical studies, antioxidants and various optimization methods in order to determine the best method of extracting curcumin extract ethanol from the plant Curcuma longa L.Eco-phytochemical Journal of Medicinal Plants, 11(3): 1-11. (In Persian)
    52.
  52. Oloumi, H., Shakeri, S. and Behzadi, M. 2016. Antioxidant activities, polyphenolic composition and their correlation analysis on Hibiscus sabdarifa L. (sabdariffa) calices,  Journal of Herbal Drugs, 7(2): 89-96.
    53.
  53. Obouayeba, A., Djyh, N., Diabate, S., Djaman, A., N’guessan, J., Kone, M. and Kouakou, T.H. 2014. Phytochemical and antioxidant activity of Roselle (Hibiscus sabdariffa L.) petal extracts. Research Journal OfPharmceutical, Biology and Chemical Sciences, 5(9): 1453-65.
    54.
  54. Podsedek, A., Wilska-Jeszka, J. and Anders, B. 2000. Compositional characterization of some apple varieties. European Food Research and Technology. 210: 268-272.
    55.
  55. Prevc, T., Segatin, N., Poklarulrih, N. and Cigic, B. 2013. DPPH assay of vegetable oils and model antioxidants in protic and aprotic solvents. Talanta, 109, 13–19.
    56.
  56. Qingming, Y., Xianhui, P., Weibao, K., Hong, Y., Yidan, S., Zhang, L., Yanan, Z., Yuling, Y., Lan, D. and Guoan, L. 2010. Antioxidant activitiesof malt extract from barley (Hordeum vulgare L.) toward variousoxidative stress invitroand invivo. Food Chemistry, 118(1): 84-89.
    57.
  57. Ramadan, K.S., Salha, A. and Alshamrani, A. 2016. Phytochemical analysis and antioxidant activity of Salvadora  persica extracts. Journal of Basic Applied Science Research,  2(3): 390-395.
    58.
  58. Sadeghi, Z., Valizadeh, J. and Azizian Shermeh, O. 2015 (a). Antioxidant activity and total phenolic content of some date varieties from Saravan Region, Baluchistan, Iran. Journal of a Medicinal Plants Research, 9(4), 78-83.
    59.
  59. Sadeghi, Z., Valizadeh, J. and Aziziam Shermeh, O. 2015 (b). Study of total phenolic and Flavonoid contents and antioxidant activity of baneh (Pistacia atlantica) Gum, from Saravan region, Sistan and Baluchestan province. Eco-phytochemical Journal of Medicinal plants, 3(2): 18-27. (In Persian)
    60.
  60. Sadeghi, Z., Valizadeh, J., Azizian Shermeh, O., Akaberi, M., 2015 (c). Antioxidant activity and total phenolic content of Boerhavia elegans (choisy) grown in Baluchistan, Iran. Avicenna Journal of Phytomededicine, 5 (1): 1-9.
    61.
  61. Sanchez, S.C., Gonzalez, G.A.M., Garcia-Parrilla, M.C., Granados, Q.J.J., Serrana, H.L.G.and Martinez, L.M.C. 2007. Different radical scavenging tests in virgin olive oil and their relation to the total phenol content. Analytica Chimica Acta, 593: 103–107.
    62.
  62. Sen, C., Ertan, F. and Isbilir, S.S. 2016. Investigation of antioxidant capacity, antimicrobial activity, total phenolic and monomeric anthocyanin contents in different extracts of Hibiscus sabdariffa L. International Journal of Biological  and  Pharmaceutical Research, 7(1): 35-41.
    63.
  63. Sharififar, F., Dehghn-Nudeh, G. and Mirtajaldini, M. 2009. Major flavonoids with antioxidant activity from Teucrium polium L. Food Chemistry. 112(4): 885–888.
    64.
  64. Sirag, N., Elhadi, MM., Algaili, M., Algaili, M., Hozeifa, M.H. and Ohaj, M. 2014. Determination of total phenolic content and antioxidant activity of Roselle (Hibiscus sabdariffa L.) Calyx ethanolic extract. Standard Research Journal of Pharmacy and Pharmacology, 1(2): 34-39.
    65.
  65. Stankovic, M.S., Stefanovic, O., Ljiljana, C.L., Topuzovic, M., Radojevic, I. and Solujic, S. 2012. Antimicrobial activity, total phenolic content and flavonoid concentrations of Teucrium species. Central European Journal of Biology, 7(4): 664-671.
    66.
  66. Tabart, J., Kevers, C., Pincemail, J., Ddefraigne, J.O. and Dommes, J. 2008. Comparative antioxidant capacities of phenolic compounds measured by various tests. Journal of Food Chemistry, 113: 1226-1233.
    67.
  67. Trusheva, B., Trunkova, D. and Bankova, V. 2007. Different extraction methods of biologically active components from propolis: a preliminary study. Chemistry Central Journal, 1(13): 1-4.
    68.
  68. Ultee, A., Bennik, M.H.J. and Moezelaar, R. 2002. The phenolic hydroxyl group of carvacrol is essential for action against the food-borne pathogen Bacillus cereus. Applied and Environmental Microbiology, 68: 1561-1568.
    69.
  69. Zhang, M., Hettiarachchy, N.S., Horax, R., Kannan, A., Praisoody, M.D.A, Muhundan, A. and Mallangi, C.R. 2011. Phytochemicals, antioxidant and antimicrobial activity of Hibiscus sabdariffa, Centella asiatica, Moringao leifera and Murraya koenigii leaves. Journal of Medicinal Plants Research, 5(30): 6672-6680.
    70.

 

 

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