• الصفحة الرئيسية
  • (مقاله کوتاه علمی) استخراج، جداسازی و تعیین ساختار شیمیایی گلیکوزید قلبی دیگوکسین از عصارۀ گیاهLeonurus cardiaca L.

شارک

عنوان URL للمقالة


رقم المقالة : EJMP-1712-1308 (R7) زيارة : 280 الصفحة: 89 - 100

20.1001.1.23223235.1398.7.3.8.5

نوع المخطوط: ابحاث

(مقاله کوتاه علمی) استخراج، جداسازی و تعیین ساختار شیمیایی گلیکوزید قلبی دیگوکسین از عصارۀ گیاهLeonurus cardiaca L.

الموضوعات :

محبوبه طاهرخانی 1 , شبنم ارجمندی 2 , مجید قربانی نهوجی 3

1 - استادیار، دانشگاه آزاد اسلامی واحد تاکستان، دانشکده علوم پایه، گروه شیمی، تاکستان، ایران
2 - دانشجوی کارشناسی ارشد، گروه آموزشی فیتوشیمی و شیمی فناوری اسانس، دانشکده شیمی دارویی، واحد علوم دارویی دانشگاه آزاد اسلامی، تهران، ایران
3 - هیات علمی مرکز تحقیقات گیاهان دارویی، پژوهشکده گیاهان دارویی جهاد دانشگاهی

تاريخ الإرسال : 13 الأحد , ربيع الثاني, 1439 تاريخ التأكيد : 28 الأحد , جمادى الأولى, 1440 تاريخ الإصدار : 25 الجمعة , ربيع الأول, 1441

الکلمات المفتاحية: ایران, کروماتوگرافی ستونی, گلیکوزید قلبی, گیاه دم شیر, منبع جدید دیگوکسین,

ملخص المقالة :

گیاه دارویی دم شیر با نام علمی Leonurus cardiaca L. گیاهی علفی و چند ساله متعلق به تیرۀ نعنائیان (Lamiaceae) است که بطور گسترده ای در طب سنتی ایران و دیگر کشورها به عنوان گیاهی آرام بخش، خواب آور، مدر، کاهنده فشار خون و بویژه به عنوان درمان بیماری‌های قلبی شناخته می‌شود. از این گیاه ترکیبات فیتوشیمیایی مهمی نظیر آلکالوییدها، ایریدوییدها، ساپونین‌ها، فلاونوییدها، کاردنولیدها و دی ترپنوییدها استخراج و شناسایی شده است. با توجه به اهمیت و پراکندگی این گیاه در ایران، هدف از این پژوهش بررسی فیتوشیمیایی عصارۀ گیاهL. cardiaca در جهت ارزیابی و شناسایی ترکیبات طبیعی موجود در آن می‌باشد. در راستای این تحقیق، ابتدا اندام‌های هوایی گیاه مذکور در مرحلۀ گلدهی از دامنه‌های کوه دماوند در مردادماه 1395 جمع آوری و به روش خیساندن در حلال عصاره گیری گردید. سپس به منظور جداسازی چربی‌ها و ترکیبات اشباع با زنجیره‌های بلند، عمل چربی‌گیری توسط حلال هگزان و در قیف جداکننده انجام شد. در مرحله بعد به منظور جداسازی فراکسیون‌های مختلف، بر روی ستون کروماتوگرافی برده و نهایتاً از میان 32 فراکسیون بدست آمده، از فراکسیون 8+9+10 بلور مربوط به ترکیبی طبیعی ظاهر گردید. برای اولین بار در دنیا از گیاه دم شیر ترکیب طبیعی یک گلیکوزید قلبی با نام دیگوکسین استخراج، جداسازی و خالص سازی شده و با استفاده از روش‌های طیف سنجی 13C-NMR، 1H-NMR، DEPT و IR ساختمان شیمیایی آن شناسایی و معرفی گردید.

المصادر:


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_||_

References

  1. Agnihotri, V.K., Elsohly, H.N., Smillie, T.J., Khan, I.A. and Walker, L.A. 2008. New labdane diterpenes from Leonurus cardiaca. Planta Medica, 74(10):1288-1290.
    2.
  2. Ali, M.S., Ibrahim, S.A., Jalil, S. and Choudhary, M.I. 2007. Ursolic acid: a potent inhibitor of superoxides produced in the cellular system. Phytotherapy Research, 21(6): 558-561.
    3.
  3. Al-Snafi, A.E. 2017. Phytochemical constituents and medicinal properties of digitalis lanata and Digitalis purpurea- A review. Indo Americal Journal of Pharmaceutical science (IAJPS), 4(2): 225-234.
    4.
  4. Bernatoniene, J., Kopustinskiene, D.M., Jakstas, V., Majiene, D., Baniene, R., Kuršvietiene, L., Masteikova, R., Savickas, A., Toleikis, A. and Trumbeckaite, S. 2014. The Effect of Leonurus cardiaca herb extract and some of its flavonoids on mitochondrial oxidative phosphorylation in the Heart. Planta Medica, 80(7): 525-532.
    5.
  5. Bernatoniene, J., Kucinskaite, A., Masteikova, R., Kalveniene, Z., Kasparaviciene, G. and Savickas, A. 2009. The comparison of anti-oxidative kinetics in vitro of the fluid extract from maidenhair tree, motherwort and hawthorn. Acta Poloniae Pharmaceutica, 66(4): 415-421.
    6.
  6. Blumenthal, M., Goldberg, A. and Brinckmann, J. 2000. Herbal medicine expanded commission e monographs. Integrative Medicine Communication. American Botanical Council: Austin, Texas, 267 p.
    7.
  7. Bol’shakova, I.V., Losovskaia, E.L., Sapezhinskii, I.I. 1997. Photosensitizing
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  8. Bradley, P.R. 1992. British Herbal Compendium (Vol. 1). British Herbal Medicine Association: Bournemouth, 161p.
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  9. Brieskorn, C.H. and Hofmann, R. 1979. Bitter substances from labiate plants: a clerodane derivative from Leonurus cardiac. Tetrahedron Letters, 20(27): 2511-2512.
    10.
  10. Calderon-Montano, J.M., Burgos-Moron, E., Luis Orta, M., Maldonado-Navas, D., Garcia-Dominguez, I. and Lopez-Lazaro, M. 2014. Evaluating the cancer therapeutic potential of cardiac glycosides. BioMed Research International, Article ID 794930. 9 p.
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  11. Chan, W.C., Wong, Y.C., Kong, Y.C., Chun, Y.T., Chang, H.T. and Chan, W.F. 1983. Clinical observation on the uterotonic effect of I-mu Ts’ao (Leonurus artemisia). American Journal of Clinical Medicine, 11: 77-83.
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  12. Chen, Z.S., Chen, C.X. and Kwan, C.Y. 2000. Leonurine, an alkaloid from Leonurus artemesia, induces contraction in mouse uterine smooth muscle but relaxation in vascular smooth muscle of rat portal vein. Biomedical Research, 11(2): 209-212.
    13.
  13. Ewy, G.A. 2015. Digoxin: The Art and Science. The American Journal of Medicine, 128(12): 1272-1274.
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  14. Feng, Y., Lu, Y., Liu, D., Zhang, W., Liu, J., Tang, H. and Zhu, Y. 2017. Apigenin-7-O-β-d-(-6″-p-coumaroyl)-glucopyranoside pretreatment attenuates myocardial ischemia/reperfusion injury via activating AMPK signaling. Life Sciences, 15(203): 246-254.
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  15. Fleming, T. 2000. PDR for herbal medicines. Medical Economics Company, Montrale, Canada, 858 p.
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  16. Flemmig, J., Noetzel, I., Arnhold, J. and Rauwald, H.W. 2015. Leonurus cardiaca L. herb extracts and their constituents promote lactoperoxidase activity. Journal of Functional Foods, 17: 328-339.
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  17. Gulubov, A. and Chervenkova, V. 1970. Structure of alkaloids from Leonurus cardiaca. Materialia Fizika Khimicheskaya Biologica, 8(1): 129-132.
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  19. Hosseini, M., Taherkhani, M. and Ghorbani Nohooji, M. 2019. Introduction of Adonis aestivalis as a new source of effective cytotoxic cardiac glycoside. Natural Product Research, 33(6): 915-920.
    20.
  20. Jafari, S., Moradi, A., Salaritabar, A., Hadjiakhoondi, A. and Khanavi, M. 2010. Determination of total phenolic and flavonoid contents of Leonurus cardiaca L. in compare with antioxidant activity. Research Journal of Biological Sciences, 5(7): 484-487.
    21.
  21. Janicsák, G., Veres, K., Kakasy, A.Z. and Máthé, I. 2006. Study of the oleanolic and ursolic acid contents of some species of the Lamiaceae. Biochemical Systematics and Ecology, 34(5): 392-396.
    22.
  22. Ji, S., Fattahi, A., Raffel, N., Hoffmann, I., Beckmann, M.W., Dittrich, R. and Schrauder, M. 2017. Antioxidant effect of aqueous extract of four plants with therapeutic potential on gynecological diseases; Semen persicae, Leonurus cardiaca, Hedyotis diffusa, and Curcuma zedoaria. European Journal of Medical Research, 22(1): 1-8.
    23.
  23. Kartnig, T., Gruber, A. and Menzinger, S. 1985. Flavonoid-O-Glycosides from the Herbs of Leonurus cardiaca. Journal of Natural Products, 48(3): 494-507.
    24.
  24. King, R.E. and Gisvold, O. 1950. Some extraction studies on Digitalis. Journal of the American Pharmaceutical Association, 39(2): 109-112.
    25.
  25. Mil kowska-Leyck, K., Filipek, B. and Strzelecka, H. 2002. Pharmacological effects of lavandulifolioside from Leonurus cardiaca. Journal of Ethnopharmacology, 80(1): 85-90.
    26.
  26. Mockute, D., Bernotiene, G. and Judzentiene, A. 2006. Germacrene D chemotype of essential oils of Leonurus cardiaca L. growing wild in Vilnius district (Lithuania). Journal of Essential Oil Research, 18(5): 566-568.
    27.
  27. Moore, W.N. and Taylor, L.T. 1996. Extraction and quantitation of digoxin and acetyldigoxin from the digitalis lanata leaf. via near-supercritical methanol-modified carbon dioxide. Journal of Natural Products, 59(7): 690-693.
    28.
  28. Morteza-Semnani, K., Saeedi, M. and Akbarzadeh, M. 2008. The essential oil composition of Leonurus cardiaca L. from Iran. Journal of Essential Oil Research, 20(2): 107-109.
    29.
  29. Mozaffarian, V. 2012. Identification of medicinal and Aromatic Plants of Iran. Farhange Moase publication, Tehran, Iran, 1444 p.
    30.
  30. Nagasawa, H., Onoyama, T., Suzuki, M., Hibino, A., Segawa, T. and Inatomi, H. 1990. Effects of motherwort (Leonurus sibiricus L.) on preneoplastic and neoplastic mammary gland growth in multiparous GR/A mice. Anticancer Research, 10:1019–1023.
    31.
  31. Novkovic, V.M., Stanojevic, L.P., Cakic, M.D., Palic, R.M., Veljkovic, V.B. and Stankovic, M.Z. 2014. Extraction of digoxin from fermented woolly foxglove foliage by percolation. Separation Science and Technology, 49(6): 829-837.
    32.
  32. Orosz, F., Nuridsány, M. and Ovádi, J. 1989. Isolation and quantitative determination of some cardioactive glycosides from Digitalis lanata by high-performance liquid chromatography. Analytical Biochemistry, 156(1): 171-175.
    33.
  33. Pang, S., Tsuchiya, S., Horie, S., Uchida, M., Murayama, T. and Watanabe, K. 2001. Enhancement of phenylephrine-induced contraction in the isolated rat aorta with endothelium by H2O-extract from an Oriental medicinal plant Leonuri Herba. Japanese Journal of Pharmacology, 86(2): 215-222.
    34.
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