Typing of the Acinetobacter baumannii strains isolated from row meat of poultry and livestock using Multi Locus Sequence Typing (MLST)
Subject Areas : Food Microbial Contamination
Marziyeh Tavakol
1
(Ph.D student of Microbiology, Department of Microbiology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran)
Hassan Momtaz
2
(Department of Microbiology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran)
Parviz Mohajeri
3
(Department of Microbiology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
Department of Microbiology, Nosocomial Infection Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran.)
Leili Shokoohizadeh
4
(Department of Microbiology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
Department of Microbiology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.)
Elahe Tajbakhsh
5
(Department of Microbiology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran)
Keywords: Antibiotic resistance, Acinetobacter baumannii, Row meat, Multi-locus Sequence Typing,
Abstract :
Acinetobacter baumannii (A. baumannii) strains with multiple drug resistance are mainly opportunistic pathogens in the development of hospital infections and as an emerging contaminant in livestock-based foods. The aim of this study was to determine the antibiotic resistance pattern and genotyping of this bacterium strains in raw meat of poultry and livestock. 22 strains isolated from raw meat were tested by multi-locus sequence typing and simple disk diffusion methods. The highest antimicrobial resistance was observed to tetracycline with 90.9% and the least antibiotic resistance was azithromycin and imipenem with 9.09%. Five strains were identified as non-typing isolates in 22 isolates of A. baumannii. Five genetic profiles (Sequence Types=ST) including ST15, ST10, ST12, ST25, ST25 were identified. Identifying the acceptable level of genetic variation among isolates using the MLST technique indicates that this method is considered as a useful method in the study and typing of Acinetobacter spp. strains and can be strains isolated from different origins in different groups. In this study, it was found that by sequencing of house-keeping genes, it is possible to typing of Acinetobacter spp. strains, and this amount of polymorphism indicates that this technique is a useful method for analyzing the genetic diversity of A. baumannii strains is a source of animal origin.
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