Evaluation of virulence and enterotoxin genes in Salmonella enteritidis strains isolated from ‎Meat and Egg samples by Multiplex-PCR

Subject Areas : Food Microbial Contamination

Toktam Khodadadipour ¹ , Kumarss Amini ² , Razagh Mahmoudi ³

1 - MSc. in Microbiology, Sirjan Branch, Islamic Azad University, Sirjan, IR Iran.
2 - Department of Microbiology, Saveh Branch, Islamic Azad University, Saveh, Iran
3 - Medical Microbiology Research Center, Qazvin University of Medical Sciences, Qazvin, Iran.

Received: 2015-12-05 Accepted : 2016-06-07 Published : 2016-10-01

Keywords: Food samples, Enterotoxin gene, Multiplex Polymerase Chain Reaction, Salmonella ‎enteritidis, Virulence gene.‎,

Abstract :

Today,food borne diseases are one of the most serious problems and occur as a result of consumption to contaminated food and water. The aim of the study was evaluation of virulence and enterotoxin genes in Salmonella enteritidis strains isolated from food samples by multiplex-PCR. This descriptive, cross-sectional study was performed at 2014 on the 1250 no duplicative and non-repetitive food samples. M-PCR assay was done in order to detection of Stn،sopB،slyA،spvc and Phop/Q genes. Sixty Salmonella enteritidis strains were obtained from poultry meat (35 strains, 58.3%) and eggs (25 strains, 41.6%), respectively. molecular analysis distribution showed all isolates (100%) were absence for spvC gene. The highest and lowest prevalence of the genes were related to Phop/Q and SopB,33.3% and 1.6%, respectively. Evaluation of virulence genes and enterotoxin in the Salmonella enteritidis isolated from the food samples are useful because of presence of the genes and efficacy of M-PCR method in epidemiological investigation and assessment of intraspecies genes transfer in food samples.