In vitro antioxidant properties and GC-MS analysis of solvent extracts of Sida acuta leaf

Subject Areas : Journal of Medicinal Herbs, "J. Med Herb" (Formerly known as Journal of Herbal Drugs or J. Herb Drug)

Akeem Yusuff ¹ , Temidayo Ogunmoyole ² , Mary Ogundare ³

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Received: 2022-08-21 Accepted : 2022-12-27 Published : 2023-02-20

Keywords: Methanol, Antioxidant, ethanol, Aqueous, Sida acuta,

Abstract :

Background & Aim: Active search for plants with diverse antioxidant and pharmacological properties capable of counteracting the deleterious effects of free radical-mediated pathologies, is still evolving. The current study investigates the phytochemical screening, antioxidant mechanisms and gas-chromatography-mass spectrometer (GC-MS) analysis of leaf extract of Sida acuta.Experimental: Powdered leaves of the plant was extracted in ethanol, methanol and water. Total phenolic and flavonoids contents of the extracts were measured using established protocols. Also, free radical scavenging activity against 2, 2-diphenyl-1-picryl hydrazyl (DPPH) and nitric oxide radicals (NO•.) as well as the ferric reducing antioxidant power (FRAP) assay were also evaluated. Furthermore, the structure and relative abundance of bioactive compounds in the methanolic leaf extract of Sida acuta was determined on GC-MS.Results: Results indicated that the total phenol and flavonoids content is significantly (P<0.05) higher in methanolic extract when compare with extracts from other solvents. Additionally, DPPH radical scavenging potency and FRAP of the extract is in the order; methanolic> aqueous> ethanolic, while the aqueous extract elicits significant NO• radical scavenging properties when compared with other solvents. The GC-MS chromatogram of the methanolic leaf extract of the plant reveals the presence of 23 bioactive compounds. Taking together, the relative abundance of phytochemical contents and antioxidant capacity of Sida acuta leaves is partly contingent on the choice of extraction solvent.Recommended applications/industries: The presence of various bioactive compounds justifies the use of the plant for diverse remedies in traditional medicine and the need for further studies.

References:

Alam, M.N., Bristi, N.J. and Rafiquzzaman, M. 2013. Review on in vivo and in vitro methods evaluation of antioxidant activity. Saudi Pharmaceutical Journal, 21:143-52.

Marston, A. 2007. Role of advances in chromatographic techniques in phytochemistry. Phytochemistry, 68: 2785-2797.

Awah, F.M., Uzoegwu, P.N., Oyugi, J.O., Rutherford, J., Ifeonu, P., Yao, X., Fowke, K.R. and Eze, M.O. 2010. Free radical scavenging activity and immunomodulatory effect of Stachytarpheta angustifolia leaf extract. Food Chemistry, 119: 1409–1416.

Aziz, M. and Karboune, S. 2018. Natural antimicrobial/antioxidant agents in meat and poultry products as well as fruits and vegetables: A review. Critical Review in Food Science and Nutrition, 58: 486–511.

Bhaskar, H.V. and Balakrishnan, N. 2009. In vitro antioxidant property of laticiferous plant species from Western Ghats Tamilnadu, India. International Journal of Health                Research, 2(2): 163–170.

Chaman, L. and Verma, L.R. 2006. Use of certain bio-products for insect-pest control. Indian Journal of Traditional Knowledge, 5(1): 79- 82.

 Chatha, S.A.S., Anwar, F., Manzoor, M. and Bajwa, J.R. 2006. Evaluation of the antioxidant activity of rice bran extracts using different antioxidant assays. Grasas Aceites Sevilla,            57: 328-335.

Choudhary, S.P. and Tran, L.S. 2011. Phytosterols: Perspectives in human nutrition and clinical therapy. Current Medicinal Chemistry, 18 (29): 4557–67.

Deepak, M. K., Surendra S. K., Mahabaleshwar, V. H. and Hanhong, B. 2015. Significance of antioxidant potential of plants and its relevance to therapeutic applications. International Journal of Biological Sciences, 2: 11-18

De-Fatima, A., Modolo, L.V., Conegero, L.S., Pilli, R.A., Ferreira, C.V., Kohn, L.K. and de-Carvalho, J.E.             2006. Lactones and their derivatives: biological activities, mechanisms of     action and potential leads for drug design. Current Medicinal Chemistry, 13: 3371-3384.

Ekpo, M.A. and Etim, P.C. 2009. Antimicrobial activity of ethanolic and aqueous extracts of Sida acuta on microorganisms from skin infections. Journal of Medicinal Plants Research, 3(9): 621–624.

Falleh, H., Ksouri, R., Lucchessi, M.E., Abdelly, C., Magné, C. 2012. Ultrasound-assisted extraction: Effect of extraction time and solvent power on the levels of polyphenols and antioxidant activity of Mesembryanthemum edule L. Aizoaceae shoots. Tropical Journal of Pharmaceutical Research, 11(2): 243-249.

 Gopalakrishnan, K. and Udayakumar, R. 2014. GC-MS analysis of phytocompounds of leaf and stem of marsilea quadrifolia (L). International Journal of Biochemistry Research and Review, 4(6): 517–526.

Halliwell, B. 2007. Biochemistry of oxidative stress. Biochemical Society Transactions, 35:1147-1150.

Hameed. I.H., Hussein, H.J., Kareem, M.A. and Hamad, N.S. 2015. Identification of five newly described bioactive chemical compounds in methanolic extract of Mentha viridis by using gas chromatography-mass spectrometry (GC-MS). Journal of Pharmacognosy and Phytotherapy, 7:107–125.

Heggen, E., Granlund, L., Pedersen, J.I., Holme, I., Ceglarek, U., Thiery, J., Kirkhus, B. and Tonstad, S. 2010. Plant sterols from rapeseed and tall oils: Effects on lipids, fat-soluble vitamins and plant sterol concentrations. Nutrition, Metabolism and Cardiovascular Diseases, 20 (4): 258–65.

Ignacimuthu, S., Ayyanar, M. and Sankara-Sivaramann, K. 2006. Ethnobotanical investigations among tribes in Madurai District of Tamil Nadu (India). Journal of Ethnobiology and Ethnomedicine, 2: 25-31.

Karadag, A., Ozcelik, B. and Saner, S. 2009. Review of methods to determine antioxidant capacities. Food Analytical Methods, 2: 41–60.

Karou, S.D., Nadembega, W.M.C., Ilboudo, D.P., Ouermi, D., Gbeassor, M., Souza, C.D. and Simpore, J. 2007. Sida acuta Burm. a medicinal plant with numerous potencies. African Journal of Biotechnology, 6(25): 2953 – 2959.

Krishnaiah, D., Sarbatly, R. and Nithyanandam, R. 2011. A review of the antioxidant potential of medicinal plant species. Food and Bioproducts Processing, 89: 217–33.

Kumari, M., Sidhartha, T., Ankur, S. and Jayabaskaran, C. 2018. Antiproliferative and antioxidative bioactive compounds in extracts of marine-derived endophytic fungus talaromyces purpureogenus. FMICB, 9:17-27.

Ksouri, R., Falleh, H., Megdiche, W., Trabelsi, N., Hamdi, B., Chaieb, K., Bakhrouf, A., Magné, C. and Abdelly, C. 2009. Antioxidant and antimicrobial activities of the edible medicinal halophyte Tamarix gallica L and related polyphenolic constituents. Food Chemical Toxicology. 47: 2083–2091.

Lakhanpal, P. and Rai, H.V. 2009. Quercetin: a versatile flavonoid. Internet Journal of Medical Update, (2)2: 22–37.

 Lucian, H., Veronica, B., Harquin, S.F., Alin, C., Ionela, L.S., Daniel, T. and Emil, A. 2014. Antioxidative             effects of the methanolic extract of Hibiscus asper leaves in mice. Romanian Biotechnological Letters, 19(3): 9376–9383.

Mann, A., Gbate, M. and Umar A.N. 2003. Sida acuta subspecies. Medicinal and economic plant of Nupeland, Jube Evanns Books and Publication.

Marcocci, L., Maguire, J.J., Droy-Lefaix, M.T. and Packer, L. 1994. The nitric oxide scavenging properties of Ginkgo biloba extract EGb761. Biochemical and Biophysical Research Communications, 201: 748-755.

Meda, A., Lamien, C. E., Romito, M., Millogo, J. and Nacoulma, O. G. 2005. Determination of the total phenolic, flavonoid and praline contents in Burkina Fasan honey, as well as their radical scavenging activity. Food Chemistry, 91: 571-577.

Milne, A, 1993. Inhalational and local anesthetics reduce tactile and thermal responses in Mimosa pudica linn. Masui, 1190-1193.

Nakkliang, K., Chaichareonku, W., Kuesap, J., Rungsihirunrat, K. 2020. Evaluation of in vitroantimalarial activity of Sida acuta Burm.f. Crude extract. Creative Science, 12(1):130-136.

Ogunmoyole, T., Rocha, J.B.T., Okoronkwo, A.E. and Kade, I.J. 2009. Altered pH homeostasis modulates the      glutathione peroxidase mimics and other antioxidant properties of diphenyl diselenide. Chemico-Biological Interactions, 182: 106–111.

Ogunmoyole, T., Alfonso, O.G., Johnson, O.D. and Yusuff, A.A. 2022. In vitro antioxidant properties and             GC-MS analysis of solvent extracts of Persea Americana. Leaf Science Letters, 3: 7-14.

Okafor, J. C. 2013. Tropical plants in health care delivery in Nigeria. Ibadan: Book builders.

 Omololu, P.A., Rocha, J.B.T. and Kade, I.J. 2009. Attachment of rhamnosyl glucoside on quercetin confers potent iron-chelating ability on its antioxidant properties. Experimental and Toxicologic Pathology, 63: 249–255.

Palaksha, M.B. and Ravishankar, K. 2012. Phytochemical screening and evaluation of in vitro antibacterial and antihelminthic activities of Sida acuta leaf extracts. Journal of     Chemical and Pharmaceutical Research, 4(11): 4757– 4761.

Paranthaman, R., Praveen, K.P. and Kumaravel, S. 2012. GC-MS analysis of phytochemicals and simultaneous determination of flavonoids in Amaranthus caudatus (Sirukeerai) by RP-HPLC. Journal of Analytical and Bioanalytical Technique, 3(1):147-157.

Pulido, R., Bravo, L. and Saura-Calixto, F. 2000. Antioxidant activity of dietary polyphenols as determined by a modified ferric reducing/antioxidant power assay. Journal of Agricultural and Food Chemistry, 48: 3396-3402.

Prior, R., L., Wu X. and Schaich, K. 2005. Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements. Journal of Agricultural and Food Chemistry, 53: 4290-4292.

Rubab, M., Ramachandran, C., Kandasamy, S., Kaliyan, B., Shuai, W., Jong-Rae, K., Daesang, Y., Myeong-Hyeon, W. and Deog-Hwan, O. 2020. Bioactive potential of 2-methoxy-4-vinylphenol and benzofuran from Brassica oleracea L. var. capitate f, rubra (Red         Cabbage) on oxidative and microbiological stability of beef meat. Foods, 9:568.

Sermakkani, M. and Thangapandian, V. 2012. GC-MS analysis of Cassia italica leaf methanol extract. Asian Journal of Pharmaceutical and Clinical Reserch, 5: 90-94.

Shareef, H. K., Muhammed, H.J., Hussein, H.M. and Hameed, I.H. 2016. Antibacterial effect of ginger (Zingiber officinale) roscoe and bioactive chemical analysis using gas chromatography mass spectrum. Oriental Journal of Chemistry, 32(2): 20-40.

Singleton, V.L., Orthofer, R. and Lamuela-Raventos, R.M. 1999. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin- Ciocalteau’s. 299: 152-170.

Tcheghebe, T. O., Ngouafong, T.F., Seukep, A.J., Kamga, J. and Nenwa, J. 2016. Ethnobotanic survey of medicinal plants used for malaria therapy in Western Cameroon. Journal of Medicinal Plants Studies, 4(3): 248-258.

Wang, L. and Weller, C.L. 2006. Recent advances in extraction of nutraceuticals from plants. Trends in Food Science and Technology, 17: 300-312.